Amino acid dipepetide frequency for Streptomyces sp. IB201691-2A2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.773AlaAla: 19.773 ± 0.131	0.995AlaCys: 0.995 ± 0.02	8.064AlaAsp: 8.064 ± 0.054	8.508AlaGlu: 8.508 ± 0.073	3.456AlaPhe: 3.456 ± 0.034	12.447AlaGly: 12.447 ± 0.07	2.77AlaHis: 2.77 ± 0.034	3.566AlaIle: 3.566 ± 0.038	2.978AlaLys: 2.978 ± 0.041	14.032AlaLeu: 14.032 ± 0.092	2.457AlaMet: 2.457 ± 0.028	2.018AlaAsn: 2.018 ± 0.026	6.66AlaPro: 6.66 ± 0.06	3.708AlaGln: 3.708 ± 0.036	9.679AlaArg: 9.679 ± 0.072	6.276AlaSer: 6.276 ± 0.044	7.075AlaThr: 7.075 ± 0.055	11.994AlaVal: 11.994 ± 0.071	1.865AlaTrp: 1.865 ± 0.029	2.824AlaTyr: 2.824 ± 0.031	0.0AlaXaa: 0.0 ± 0.0
Cys	1.036CysAla: 1.036 ± 0.019	0.084CysCys: 0.084 ± 0.006	0.465CysAsp: 0.465 ± 0.011	0.403CysGlu: 0.403 ± 0.011	0.206CysPhe: 0.206 ± 0.007	0.907CysGly: 0.907 ± 0.019	0.179CysHis: 0.179 ± 0.008	0.168CysIle: 0.168 ± 0.008	0.109CysLys: 0.109 ± 0.006	0.734CysLeu: 0.734 ± 0.014	0.12CysMet: 0.12 ± 0.007	0.139CysAsn: 0.139 ± 0.007	0.447CysPro: 0.447 ± 0.013	0.161CysGln: 0.161 ± 0.009	0.564CysArg: 0.564 ± 0.013	0.425CysSer: 0.425 ± 0.013	0.497CysThr: 0.497 ± 0.012	0.638CysVal: 0.638 ± 0.013	0.122CysTrp: 0.122 ± 0.007	0.146CysTyr: 0.146 ± 0.007	0.0CysXaa: 0.0 ± 0.0
Asp	7.345AspAla: 7.345 ± 0.051	0.412AspCys: 0.412 ± 0.013	3.676AspAsp: 3.676 ± 0.038	3.991AspGlu: 3.991 ± 0.035	1.696AspPhe: 1.696 ± 0.026	6.44AspGly: 6.44 ± 0.058	1.436AspHis: 1.436 ± 0.022	1.996AspIle: 1.996 ± 0.029	1.321AspLys: 1.321 ± 0.026	6.143AspLeu: 6.143 ± 0.047	0.85AspMet: 0.85 ± 0.016	1.055AspAsn: 1.055 ± 0.022	4.378AspPro: 4.378 ± 0.04	1.623AspGln: 1.623 ± 0.024	4.781AspArg: 4.781 ± 0.045	2.878AspSer: 2.878 ± 0.032	3.424AspThr: 3.424 ± 0.034	4.783AspVal: 4.783 ± 0.044	1.079AspTrp: 1.079 ± 0.02	1.135AspTyr: 1.135 ± 0.022	0.0AspXaa: 0.0 ± 0.0
Glu	7.261GluAla: 7.261 ± 0.061	0.355GluCys: 0.355 ± 0.011	2.85GluAsp: 2.85 ± 0.033	3.517GluGlu: 3.517 ± 0.039	1.532GluPhe: 1.532 ± 0.022	4.491GluGly: 4.491 ± 0.041	1.496GluHis: 1.496 ± 0.024	2.238GluIle: 2.238 ± 0.033	1.547GluLys: 1.547 ± 0.028	6.83GluLeu: 6.83 ± 0.058	0.898GluMet: 0.898 ± 0.019	1.088GluAsn: 1.088 ± 0.02	3.4GluPro: 3.4 ± 0.037	2.266GluGln: 2.266 ± 0.029	5.305GluArg: 5.305 ± 0.045	2.786GluSer: 2.786 ± 0.032	2.962GluThr: 2.962 ± 0.03	4.395GluVal: 4.395 ± 0.04	0.804GluTrp: 0.804 ± 0.015	1.168GluTyr: 1.168 ± 0.019	0.0GluXaa: 0.0 ± 0.0
Phe	3.629PheAla: 3.629 ± 0.034	0.245PheCys: 0.245 ± 0.008	1.964PheAsp: 1.964 ± 0.028	1.433PheGlu: 1.433 ± 0.025	0.882PhePhe: 0.882 ± 0.017	3.079PheGly: 3.079 ± 0.035	0.609PheHis: 0.609 ± 0.015	0.757PheIle: 0.757 ± 0.018	0.565PheLys: 0.565 ± 0.014	2.615PheLeu: 2.615 ± 0.031	0.426PheMet: 0.426 ± 0.013	0.606PheAsn: 0.606 ± 0.015	1.402PhePro: 1.402 ± 0.024	0.699PheGln: 0.699 ± 0.016	1.837PheArg: 1.837 ± 0.023	1.523PheSer: 1.523 ± 0.027	2.057PheThr: 2.057 ± 0.026	2.277PheVal: 2.277 ± 0.025	0.447PheTrp: 0.447 ± 0.012	0.59PheTyr: 0.59 ± 0.016	0.0PheXaa: 0.0 ± 0.0
Gly	10.718GlyAla: 10.718 ± 0.069	0.812GlyCys: 0.812 ± 0.018	5.255GlyAsp: 5.255 ± 0.05	5.109GlyGlu: 5.109 ± 0.04	2.95GlyPhe: 2.95 ± 0.032	8.805GlyGly: 8.805 ± 0.083	2.261GlyHis: 2.261 ± 0.026	3.59GlyIle: 3.59 ± 0.032	2.555GlyLys: 2.555 ± 0.038	9.456GlyLeu: 9.456 ± 0.063	1.994GlyMet: 1.994 ± 0.026	1.916GlyAsn: 1.916 ± 0.03	5.159GlyPro: 5.159 ± 0.058	2.771GlyGln: 2.771 ± 0.033	7.485GlyArg: 7.485 ± 0.055	5.722GlySer: 5.722 ± 0.047	6.488GlyThr: 6.488 ± 0.056	7.455GlyVal: 7.455 ± 0.056	1.705GlyTrp: 1.705 ± 0.024	2.254GlyTyr: 2.254 ± 0.025	0.0GlyXaa: 0.0 ± 0.0
His	2.586HisAla: 2.586 ± 0.028	0.199HisCys: 0.199 ± 0.008	1.396HisAsp: 1.396 ± 0.024	1.255HisGlu: 1.255 ± 0.018	0.656HisPhe: 0.656 ± 0.016	2.354HisGly: 2.354 ± 0.034	0.682HisHis: 0.682 ± 0.018	0.715HisIle: 0.715 ± 0.014	0.376HisLys: 0.376 ± 0.01	2.337HisLeu: 2.337 ± 0.03	0.328HisMet: 0.328 ± 0.008	0.404HisAsn: 0.404 ± 0.012	1.784HisPro: 1.784 ± 0.027	0.649HisGln: 0.649 ± 0.016	2.051HisArg: 2.051 ± 0.028	1.057HisSer: 1.057 ± 0.019	1.358HisThr: 1.358 ± 0.022	1.655HisVal: 1.655 ± 0.022	0.382HisTrp: 0.382 ± 0.011	0.492HisTyr: 0.492 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	4.836IleAla: 4.836 ± 0.046	0.287IleCys: 0.287 ± 0.009	2.303IleAsp: 2.303 ± 0.029	2.009IleGlu: 2.009 ± 0.029	0.752IlePhe: 0.752 ± 0.019	3.617IleGly: 3.617 ± 0.04	0.65IleHis: 0.65 ± 0.015	0.887IleIle: 0.887 ± 0.019	0.761IleLys: 0.761 ± 0.017	2.492IleLeu: 2.492 ± 0.03	0.455IleMet: 0.455 ± 0.013	0.726IleAsn: 0.726 ± 0.017	1.873IlePro: 1.873 ± 0.024	0.749IleGln: 0.749 ± 0.017	2.327IleArg: 2.327 ± 0.029	1.746IleSer: 1.746 ± 0.022	2.317IleThr: 2.317 ± 0.026	2.807IleVal: 2.807 ± 0.034	0.399IleTrp: 0.399 ± 0.011	0.534IleTyr: 0.534 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	3.067LysAla: 3.067 ± 0.043	0.123LysCys: 0.123 ± 0.007	1.497LysAsp: 1.497 ± 0.027	1.284LysGlu: 1.284 ± 0.025	0.481LysPhe: 0.481 ± 0.013	2.011LysGly: 2.011 ± 0.035	0.458LysHis: 0.458 ± 0.011	0.892LysIle: 0.892 ± 0.02	0.96LysLys: 0.96 ± 0.029	2.112LysLeu: 2.112 ± 0.03	0.398LysMet: 0.398 ± 0.013	0.553LysAsn: 0.553 ± 0.015	1.43LysPro: 1.43 ± 0.024	0.759LysGln: 0.759 ± 0.018	1.493LysArg: 1.493 ± 0.021	1.304LysSer: 1.304 ± 0.022	1.376LysThr: 1.376 ± 0.027	2.001LysVal: 2.001 ± 0.03	0.32LysTrp: 0.32 ± 0.011	0.515LysTyr: 0.515 ± 0.014	0.0LysXaa: 0.0 ± 0.0
Leu	14.685LeuAla: 14.685 ± 0.092	0.81LeuCys: 0.81 ± 0.016	6.671LeuAsp: 6.671 ± 0.055	4.559LeuGlu: 4.559 ± 0.043	2.648LeuPhe: 2.648 ± 0.035	9.226LeuGly: 9.226 ± 0.062	2.274LeuHis: 2.274 ± 0.027	3.384LeuIle: 3.384 ± 0.034	2.219LeuLys: 2.219 ± 0.031	11.165LeuLeu: 11.165 ± 0.086	1.704LeuMet: 1.704 ± 0.023	1.779LeuAsn: 1.779 ± 0.022	6.342LeuPro: 6.342 ± 0.053	2.239LeuGln: 2.239 ± 0.029	8.43LeuArg: 8.43 ± 0.061	5.498LeuSer: 5.498 ± 0.042	6.894LeuThr: 6.894 ± 0.049	8.794LeuVal: 8.794 ± 0.065	1.305LeuTrp: 1.305 ± 0.021	1.892LeuTyr: 1.892 ± 0.027	0.0LeuXaa: 0.0 ± 0.0
Met	2.247MetAla: 2.247 ± 0.03	0.127MetCys: 0.127 ± 0.007	0.89MetAsp: 0.89 ± 0.017	0.78MetGlu: 0.78 ± 0.017	0.456MetPhe: 0.456 ± 0.011	1.351MetGly: 1.351 ± 0.019	0.377MetHis: 0.377 ± 0.011	0.674MetIle: 0.674 ± 0.017	0.454MetLys: 0.454 ± 0.012	1.704MetLeu: 1.704 ± 0.025	0.316MetMet: 0.316 ± 0.009	0.452MetAsn: 0.452 ± 0.012	1.157MetPro: 1.157 ± 0.022	0.455MetGln: 0.455 ± 0.013	1.447MetArg: 1.447 ± 0.021	1.341MetSer: 1.341 ± 0.023	1.55MetThr: 1.55 ± 0.021	1.291MetVal: 1.291 ± 0.022	0.215MetTrp: 0.215 ± 0.009	0.334MetTyr: 0.334 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	2.264AsnAla: 2.264 ± 0.028	0.17AsnCys: 0.17 ± 0.007	1.068AsnAsp: 1.068 ± 0.02	0.888AsnGlu: 0.888 ± 0.018	0.512AsnPhe: 0.512 ± 0.013	2.058AsnGly: 2.058 ± 0.036	0.422AsnHis: 0.422 ± 0.013	0.68AsnIle: 0.68 ± 0.014	0.451AsnLys: 0.451 ± 0.015	1.687AsnLeu: 1.687 ± 0.026	0.315AsnMet: 0.315 ± 0.009	0.467AsnAsn: 0.467 ± 0.013	1.437AsnPro: 1.437 ± 0.024	0.557AsnGln: 0.557 ± 0.015	1.316AsnArg: 1.316 ± 0.02	1.132AsnSer: 1.132 ± 0.022	1.217AsnThr: 1.217 ± 0.022	1.437AsnVal: 1.437 ± 0.023	0.333AsnTrp: 0.333 ± 0.011	0.459AsnTyr: 0.459 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	7.962ProAla: 7.962 ± 0.059	0.317ProCys: 0.317 ± 0.009	4.49ProAsp: 4.49 ± 0.043	4.356ProGlu: 4.356 ± 0.035	1.567ProPhe: 1.567 ± 0.021	6.515ProGly: 6.515 ± 0.057	1.371ProHis: 1.371 ± 0.019	1.317ProIle: 1.317 ± 0.021	1.285ProLys: 1.285 ± 0.022	5.266ProLeu: 5.266 ± 0.05	1.029ProMet: 1.029 ± 0.018	0.98ProAsn: 0.98 ± 0.017	3.44ProPro: 3.44 ± 0.048	1.751ProGln: 1.751 ± 0.034	3.859ProArg: 3.859 ± 0.039	3.381ProSer: 3.381 ± 0.038	3.32ProThr: 3.32 ± 0.041	5.404ProVal: 5.404 ± 0.046	0.917ProTrp: 0.917 ± 0.02	1.403ProTyr: 1.403 ± 0.024	0.0ProXaa: 0.0 ± 0.0
Gln	3.617GlnAla: 3.617 ± 0.035	0.172GlnCys: 0.172 ± 0.008	1.477GlnAsp: 1.477 ± 0.024	1.51GlnGlu: 1.51 ± 0.021	0.706GlnPhe: 0.706 ± 0.015	2.33GlnGly: 2.33 ± 0.03	0.659GlnHis: 0.659 ± 0.017	1.084GlnIle: 1.084 ± 0.021	0.64GlnLys: 0.64 ± 0.015	3.221GlnLeu: 3.221 ± 0.028	0.512GlnMet: 0.512 ± 0.012	0.551GlnAsn: 0.551 ± 0.016	1.726GlnPro: 1.726 ± 0.033	1.309GlnGln: 1.309 ± 0.026	2.377GlnArg: 2.377 ± 0.027	1.361GlnSer: 1.361 ± 0.022	1.401GlnThr: 1.401 ± 0.024	2.35GlnVal: 2.35 ± 0.028	0.489GlnTrp: 0.489 ± 0.014	0.631GlnTyr: 0.631 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	9.361ArgAla: 9.361 ± 0.069	0.568ArgCys: 0.568 ± 0.014	4.212ArgAsp: 4.212 ± 0.042	4.653ArgGlu: 4.653 ± 0.042	2.309ArgPhe: 2.309 ± 0.027	5.695ArgGly: 5.695 ± 0.045	2.079ArgHis: 2.079 ± 0.026	3.274ArgIle: 3.274 ± 0.035	1.662ArgLys: 1.662 ± 0.026	8.511ArgLeu: 8.511 ± 0.064	1.69ArgMet: 1.69 ± 0.024	1.409ArgAsn: 1.409 ± 0.025	4.773ArgPro: 4.773 ± 0.041	2.29ArgGln: 2.29 ± 0.028	7.478ArgArg: 7.478 ± 0.062	4.138ArgSer: 4.138 ± 0.044	5.442ArgThr: 5.442 ± 0.046	5.787ArgVal: 5.787 ± 0.044	1.367ArgTrp: 1.367 ± 0.021	1.777ArgTyr: 1.777 ± 0.025	0.0ArgXaa: 0.0 ± 0.0
Ser	7.016SerAla: 7.016 ± 0.053	0.397SerCys: 0.397 ± 0.012	2.953SerAsp: 2.953 ± 0.036	2.672SerGlu: 2.672 ± 0.03	1.566SerPhe: 1.566 ± 0.024	6.321SerGly: 6.321 ± 0.06	1.084SerHis: 1.084 ± 0.018	1.528SerIle: 1.528 ± 0.022	1.182SerLys: 1.182 ± 0.023	5.014SerLeu: 5.014 ± 0.042	1.131SerMet: 1.131 ± 0.019	0.982SerAsn: 0.982 ± 0.02	3.363SerPro: 3.363 ± 0.038	1.337SerGln: 1.337 ± 0.022	3.789SerArg: 3.789 ± 0.038	3.193SerSer: 3.193 ± 0.042	3.321SerThr: 3.321 ± 0.033	4.482SerVal: 4.482 ± 0.04	0.964SerTrp: 0.964 ± 0.02	1.298SerTyr: 1.298 ± 0.019	0.0SerXaa: 0.0 ± 0.0
Thr	8.863ThrAla: 8.863 ± 0.06	0.417ThrCys: 0.417 ± 0.011	3.787ThrAsp: 3.787 ± 0.04	3.369ThrGlu: 3.369 ± 0.035	1.665ThrPhe: 1.665 ± 0.022	6.754ThrGly: 6.754 ± 0.06	1.247ThrHis: 1.247 ± 0.019	1.774ThrIle: 1.774 ± 0.024	1.325ThrLys: 1.325 ± 0.025	5.831ThrLeu: 5.831 ± 0.048	0.961ThrMet: 0.961 ± 0.019	1.11ThrAsn: 1.11 ± 0.019	4.168ThrPro: 4.168 ± 0.039	1.412ThrGln: 1.412 ± 0.024	3.986ThrArg: 3.986 ± 0.035	3.411ThrSer: 3.411 ± 0.038	4.019ThrThr: 4.019 ± 0.038	6.215ThrVal: 6.215 ± 0.047	0.958ThrTrp: 0.958 ± 0.019	1.457ThrTyr: 1.457 ± 0.023	0.0ThrXaa: 0.0 ± 0.0
Val	10.438ValAla: 10.438 ± 0.059	0.728ValCys: 0.728 ± 0.017	5.021ValAsp: 5.021 ± 0.043	4.74ValGlu: 4.74 ± 0.042	2.44ValPhe: 2.44 ± 0.032	6.632ValGly: 6.632 ± 0.05	1.924ValHis: 1.924 ± 0.026	2.882ValIle: 2.882 ± 0.033	1.809ValLys: 1.809 ± 0.03	9.476ValLeu: 9.476 ± 0.069	1.459ValMet: 1.459 ± 0.022	1.721ValAsn: 1.721 ± 0.027	5.124ValPro: 5.124 ± 0.046	2.128ValGln: 2.128 ± 0.024	7.112ValArg: 7.112 ± 0.055	4.418ValSer: 4.418 ± 0.038	5.668ValThr: 5.668 ± 0.044	7.98ValVal: 7.98 ± 0.064	1.209ValTrp: 1.209 ± 0.021	1.565ValTyr: 1.565 ± 0.022	0.0ValXaa: 0.0 ± 0.0
Trp	1.659TrpAla: 1.659 ± 0.025	0.153TrpCys: 0.153 ± 0.007	0.885TrpAsp: 0.885 ± 0.017	0.767TrpGlu: 0.767 ± 0.016	0.5TrpPhe: 0.5 ± 0.012	1.101TrpGly: 1.101 ± 0.021	0.374TrpHis: 0.374 ± 0.012	0.596TrpIle: 0.596 ± 0.013	0.394TrpLys: 0.394 ± 0.011	1.809TrpLeu: 1.809 ± 0.028	0.303TrpMet: 0.303 ± 0.01	0.448TrpAsn: 0.448 ± 0.014	0.8TrpPro: 0.8 ± 0.015	0.64TrpGln: 0.64 ± 0.015	1.335TrpArg: 1.335 ± 0.022	0.972TrpSer: 0.972 ± 0.019	1.126TrpThr: 1.126 ± 0.02	1.001TrpVal: 1.001 ± 0.017	0.363TrpTrp: 0.363 ± 0.012	0.386TrpTyr: 0.386 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.78TyrAla: 2.78 ± 0.032	0.177TyrCys: 0.177 ± 0.008	1.518TyrAsp: 1.518 ± 0.026	1.384TyrGlu: 1.384 ± 0.021	0.665TyrPhe: 0.665 ± 0.017	2.323TyrGly: 2.323 ± 0.033	0.367TyrHis: 0.367 ± 0.011	0.526TyrIle: 0.526 ± 0.012	0.441TyrLys: 0.441 ± 0.013	2.081TyrLeu: 2.081 ± 0.03	0.273TyrMet: 0.273 ± 0.01	0.463TyrAsn: 0.463 ± 0.013	1.067TyrPro: 1.067 ± 0.021	0.619TyrGln: 0.619 ± 0.014	1.827TyrArg: 1.827 ± 0.028	1.033TyrSer: 1.033 ± 0.019	1.216TyrThr: 1.216 ± 0.021	1.729TyrVal: 1.729 ± 0.022	0.373TyrTrp: 0.373 ± 0.013	0.498TyrTyr: 0.498 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 9596 proteins (3162214 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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