Mycobacterium phage OKaNui

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 87 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D4XKK5|A0A7D4XKK5_9CAUD Uncharacterized protein OS=Mycobacterium phage OKaNui OX=2743844 GN=59 PE=4 SV=1
MM1 pKa = 7.4KK2 pKa = 10.52LVTALLLLAVVLGLTACEE20 pKa = 4.21GDD22 pKa = 3.92SGGSDD27 pKa = 3.46YY28 pKa = 11.41DD29 pKa = 3.77GPNGVIFMPVQGNPVGIPIFFF50 pKa = 4.82

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D4XKJ8|A0A7D4XKJ8_9CAUD Head-to-tail adaptor OS=Mycobacterium phage OKaNui OX=2743844 GN=18 PE=4 SV=1
MM1 pKa = 7.29TAMLDD6 pKa = 3.55RR7 pKa = 11.84PTTMTPPQARR17 pKa = 11.84FQARR21 pKa = 11.84SLMDD25 pKa = 3.15EE26 pKa = 4.96HH27 pKa = 7.31GLQDD31 pKa = 2.86WHH33 pKa = 8.01LRR35 pKa = 11.84FDD37 pKa = 3.66NAKK40 pKa = 10.01RR41 pKa = 11.84RR42 pKa = 11.84AGQCNYY48 pKa = 9.85RR49 pKa = 11.84DD50 pKa = 3.66RR51 pKa = 11.84TISLSLHH58 pKa = 5.93LLRR61 pKa = 11.84LRR63 pKa = 11.84SAEE66 pKa = 4.0DD67 pKa = 3.25TMQTITHH74 pKa = 7.23EE75 pKa = 4.13IAHH78 pKa = 6.42ALVGPGHH85 pKa = 5.68GHH87 pKa = 6.92DD88 pKa = 3.91YY89 pKa = 10.36VWARR93 pKa = 11.84KK94 pKa = 8.98HH95 pKa = 6.74RR96 pKa = 11.84EE97 pKa = 3.7LGGNGQRR104 pKa = 11.84CFEE107 pKa = 4.68MEE109 pKa = 5.75DD110 pKa = 3.51IDD112 pKa = 5.35PTAPWIGTCSHH123 pKa = 6.43GKK125 pKa = 9.0QFARR129 pKa = 11.84YY130 pKa = 7.6RR131 pKa = 11.84QPKK134 pKa = 9.23RR135 pKa = 11.84LEE137 pKa = 3.72GWRR140 pKa = 11.84CRR142 pKa = 11.84CRR144 pKa = 11.84PSSSPVVWKK153 pKa = 10.78KK154 pKa = 10.75RR155 pKa = 11.84GG156 pKa = 3.26

Molecular weight: 18.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

87

0

87

16017

28

866

184.1

20.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.733 ± 0.412

0.83 ± 0.101

6.256 ± 0.206

6.574 ± 0.293

3.309 ± 0.192

8.841 ± 0.468

1.973 ± 0.2

4.651 ± 0.174

4.464 ± 0.289

8.429 ± 0.252

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.51 ± 0.114

3.296 ± 0.152

5.725 ± 0.312

3.596 ± 0.219

6.749 ± 0.345

5.438 ± 0.2

5.538 ± 0.21

7.117 ± 0.211

2.06 ± 0.142

2.909 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski