Amino acid dipepetide frequency for Mycobacterium phage OKaNui

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.051AlaAla: 11.051 ± 1.067
0.812AlaCys: 0.812 ± 0.251
4.745AlaAsp: 4.745 ± 0.606
6.431AlaGlu: 6.431 ± 0.615
3.122AlaPhe: 3.122 ± 0.34
7.867AlaGly: 7.867 ± 0.723
1.686AlaHis: 1.686 ± 0.309
5.245AlaIle: 5.245 ± 0.576
4.308AlaLys: 4.308 ± 0.651
9.178AlaLeu: 9.178 ± 0.871
3.184AlaMet: 3.184 ± 0.512
2.935AlaAsn: 2.935 ± 0.538
5.245AlaPro: 5.245 ± 0.714
4.183AlaGln: 4.183 ± 0.566
5.994AlaArg: 5.994 ± 0.671
4.246AlaSer: 4.246 ± 0.536
5.182AlaThr: 5.182 ± 0.502
7.617AlaVal: 7.617 ± 0.614
2.06AlaTrp: 2.06 ± 0.364
2.248AlaTyr: 2.248 ± 0.523
0.0AlaXaa: 0.0 ± 0.0
Cys
0.749CysAla: 0.749 ± 0.201
0.187CysCys: 0.187 ± 0.141
0.5CysAsp: 0.5 ± 0.156
0.562CysGlu: 0.562 ± 0.161
0.25CysPhe: 0.25 ± 0.113
0.812CysGly: 0.812 ± 0.199
0.312CysHis: 0.312 ± 0.128
0.187CysIle: 0.187 ± 0.099
0.187CysLys: 0.187 ± 0.108
0.687CysLeu: 0.687 ± 0.261
0.312CysMet: 0.312 ± 0.135
0.5CysAsn: 0.5 ± 0.178
0.437CysPro: 0.437 ± 0.192
0.312CysGln: 0.312 ± 0.15
0.624CysArg: 0.624 ± 0.237
0.312CysSer: 0.312 ± 0.129
0.375CysThr: 0.375 ± 0.162
0.5CysVal: 0.5 ± 0.179
0.25CysTrp: 0.25 ± 0.151
0.25CysTyr: 0.25 ± 0.108
0.0CysXaa: 0.0 ± 0.0
Asp
6.181AspAla: 6.181 ± 0.619
0.437AspCys: 0.437 ± 0.174
3.372AspAsp: 3.372 ± 0.489
4.496AspGlu: 4.496 ± 0.509
1.873AspPhe: 1.873 ± 0.352
5.807AspGly: 5.807 ± 0.652
1.561AspHis: 1.561 ± 0.368
2.56AspIle: 2.56 ± 0.36
2.248AspLys: 2.248 ± 0.374
5.495AspLeu: 5.495 ± 0.612
1.374AspMet: 1.374 ± 0.265
1.811AspAsn: 1.811 ± 0.342
4.558AspPro: 4.558 ± 0.689
1.873AspGln: 1.873 ± 0.294
3.996AspArg: 3.996 ± 0.56
3.184AspSer: 3.184 ± 0.415
3.372AspThr: 3.372 ± 0.497
4.246AspVal: 4.246 ± 0.426
1.623AspTrp: 1.623 ± 0.289
2.498AspTyr: 2.498 ± 0.361
0.0AspXaa: 0.0 ± 0.0
Glu
6.618GluAla: 6.618 ± 0.758
0.687GluCys: 0.687 ± 0.183
4.683GluAsp: 4.683 ± 0.643
4.808GluGlu: 4.808 ± 0.637
2.435GluPhe: 2.435 ± 0.361
5.619GluGly: 5.619 ± 0.584
1.061GluHis: 1.061 ± 0.287
2.06GluIle: 2.06 ± 0.424
2.435GluLys: 2.435 ± 0.44
7.305GluLeu: 7.305 ± 0.649
1.124GluMet: 1.124 ± 0.317
2.31GluAsn: 2.31 ± 0.462
3.059GluPro: 3.059 ± 0.498
2.56GluGln: 2.56 ± 0.411
4.745GluArg: 4.745 ± 0.53
3.621GluSer: 3.621 ± 0.467
2.872GluThr: 2.872 ± 0.386
4.62GluVal: 4.62 ± 0.56
1.436GluTrp: 1.436 ± 0.317
1.686GluTyr: 1.686 ± 0.345
0.0GluXaa: 0.0 ± 0.0
Phe
3.871PheAla: 3.871 ± 0.47
0.187PheCys: 0.187 ± 0.086
2.498PheAsp: 2.498 ± 0.421
2.498PheGlu: 2.498 ± 0.454
0.687PhePhe: 0.687 ± 0.203
3.434PheGly: 3.434 ± 0.47
0.624PheHis: 0.624 ± 0.198
1.811PheIle: 1.811 ± 0.29
1.186PheLys: 1.186 ± 0.222
2.622PheLeu: 2.622 ± 0.537
0.687PheMet: 0.687 ± 0.196
1.186PheAsn: 1.186 ± 0.307
2.06PhePro: 2.06 ± 0.424
0.874PheGln: 0.874 ± 0.22
1.623PheArg: 1.623 ± 0.246
1.873PheSer: 1.873 ± 0.323
1.686PheThr: 1.686 ± 0.312
2.81PheVal: 2.81 ± 0.451
0.312PheTrp: 0.312 ± 0.158
0.562PheTyr: 0.562 ± 0.187
0.0PheXaa: 0.0 ± 0.0
Gly
5.869GlyAla: 5.869 ± 0.612
0.749GlyCys: 0.749 ± 0.203
5.619GlyAsp: 5.619 ± 0.71
4.683GlyGlu: 4.683 ± 0.528
3.934GlyPhe: 3.934 ± 0.677
8.367GlyGly: 8.367 ± 1.472
2.248GlyHis: 2.248 ± 0.408
4.808GlyIle: 4.808 ± 0.647
5.182GlyLys: 5.182 ± 0.635
7.43GlyLeu: 7.43 ± 0.722
2.435GlyMet: 2.435 ± 0.356
3.809GlyAsn: 3.809 ± 0.818
5.12GlyPro: 5.12 ± 1.241
2.747GlyGln: 2.747 ± 0.453
4.808GlyArg: 4.808 ± 0.529
5.245GlySer: 5.245 ± 0.692
5.057GlyThr: 5.057 ± 0.754
5.557GlyVal: 5.557 ± 0.667
2.06GlyTrp: 2.06 ± 0.352
2.685GlyTyr: 2.685 ± 0.373
0.0GlyXaa: 0.0 ± 0.0
His
1.748HisAla: 1.748 ± 0.334
0.187HisCys: 0.187 ± 0.105
0.999HisAsp: 0.999 ± 0.239
1.374HisGlu: 1.374 ± 0.249
0.812HisPhe: 0.812 ± 0.236
1.936HisGly: 1.936 ± 0.447
0.437HisHis: 0.437 ± 0.157
0.937HisIle: 0.937 ± 0.24
0.749HisLys: 0.749 ± 0.208
1.748HisLeu: 1.748 ± 0.334
0.375HisMet: 0.375 ± 0.165
0.187HisAsn: 0.187 ± 0.091
1.436HisPro: 1.436 ± 0.295
0.687HisGln: 0.687 ± 0.19
1.936HisArg: 1.936 ± 0.356
0.624HisSer: 0.624 ± 0.257
1.436HisThr: 1.436 ± 0.347
0.937HisVal: 0.937 ± 0.236
0.5HisTrp: 0.5 ± 0.184
0.687HisTyr: 0.687 ± 0.204
0.0HisXaa: 0.0 ± 0.0
Ile
5.182IleAla: 5.182 ± 0.6
0.375IleCys: 0.375 ± 0.139
3.684IleAsp: 3.684 ± 0.478
3.309IleGlu: 3.309 ± 0.481
0.749IlePhe: 0.749 ± 0.222
4.246IleGly: 4.246 ± 0.495
0.812IleHis: 0.812 ± 0.193
1.561IleIle: 1.561 ± 0.344
1.811IleLys: 1.811 ± 0.294
3.434IleLeu: 3.434 ± 0.425
0.874IleMet: 0.874 ± 0.209
1.436IleAsn: 1.436 ± 0.323
3.559IlePro: 3.559 ± 0.429
1.436IleGln: 1.436 ± 0.451
3.559IleArg: 3.559 ± 0.455
1.811IleSer: 1.811 ± 0.361
2.872IleThr: 2.872 ± 0.415
4.058IleVal: 4.058 ± 0.506
0.437IleTrp: 0.437 ± 0.172
1.311IleTyr: 1.311 ± 0.285
0.0IleXaa: 0.0 ± 0.0
Lys
4.745LysAla: 4.745 ± 0.583
0.25LysCys: 0.25 ± 0.123
2.373LysAsp: 2.373 ± 0.453
2.373LysGlu: 2.373 ± 0.416
1.311LysPhe: 1.311 ± 0.299
4.183LysGly: 4.183 ± 0.594
1.249LysHis: 1.249 ± 0.258
2.248LysIle: 2.248 ± 0.62
2.185LysLys: 2.185 ± 0.372
3.871LysLeu: 3.871 ± 0.426
0.562LysMet: 0.562 ± 0.216
1.623LysAsn: 1.623 ± 0.263
2.622LysPro: 2.622 ± 0.428
1.623LysGln: 1.623 ± 0.301
2.997LysArg: 2.997 ± 0.528
2.373LysSer: 2.373 ± 0.421
2.622LysThr: 2.622 ± 0.375
3.122LysVal: 3.122 ± 0.54
1.124LysTrp: 1.124 ± 0.308
1.186LysTyr: 1.186 ± 0.227
0.0LysXaa: 0.0 ± 0.0
Leu
9.241LeuAla: 9.241 ± 0.881
0.687LeuCys: 0.687 ± 0.183
5.619LeuAsp: 5.619 ± 0.604
6.244LeuGlu: 6.244 ± 0.607
2.56LeuPhe: 2.56 ± 0.444
6.868LeuGly: 6.868 ± 0.671
1.186LeuHis: 1.186 ± 0.348
4.371LeuIle: 4.371 ± 0.527
3.309LeuLys: 3.309 ± 0.621
5.869LeuLeu: 5.869 ± 0.598
2.935LeuMet: 2.935 ± 0.45
2.935LeuAsn: 2.935 ± 0.416
4.371LeuPro: 4.371 ± 0.465
2.498LeuGln: 2.498 ± 0.433
6.868LeuArg: 6.868 ± 0.658
5.37LeuSer: 5.37 ± 0.642
4.496LeuThr: 4.496 ± 0.633
5.182LeuVal: 5.182 ± 0.509
1.311LeuTrp: 1.311 ± 0.297
2.373LeuTyr: 2.373 ± 0.418
0.0LeuXaa: 0.0 ± 0.0
Met
2.56MetAla: 2.56 ± 0.349
0.125MetCys: 0.125 ± 0.091
1.811MetAsp: 1.811 ± 0.352
1.124MetGlu: 1.124 ± 0.249
0.937MetPhe: 0.937 ± 0.27
1.998MetGly: 1.998 ± 0.351
0.437MetHis: 0.437 ± 0.165
0.874MetIle: 0.874 ± 0.237
1.436MetLys: 1.436 ± 0.288
1.499MetLeu: 1.499 ± 0.326
0.312MetMet: 0.312 ± 0.111
0.624MetAsn: 0.624 ± 0.189
1.873MetPro: 1.873 ± 0.293
0.874MetGln: 0.874 ± 0.206
1.873MetArg: 1.873 ± 0.331
1.936MetSer: 1.936 ± 0.347
1.998MetThr: 1.998 ± 0.307
1.623MetVal: 1.623 ± 0.312
0.437MetTrp: 0.437 ± 0.18
0.749MetTyr: 0.749 ± 0.192
0.0MetXaa: 0.0 ± 0.0
Asn
3.434AsnAla: 3.434 ± 0.45
0.437AsnCys: 0.437 ± 0.141
1.748AsnAsp: 1.748 ± 0.302
1.561AsnGlu: 1.561 ± 0.303
0.624AsnPhe: 0.624 ± 0.194
3.934AsnGly: 3.934 ± 0.582
0.937AsnHis: 0.937 ± 0.279
1.311AsnIle: 1.311 ± 0.298
1.249AsnLys: 1.249 ± 0.279
2.498AsnLeu: 2.498 ± 0.346
0.562AsnMet: 0.562 ± 0.177
0.437AsnAsn: 0.437 ± 0.159
2.622AsnPro: 2.622 ± 0.462
1.186AsnGln: 1.186 ± 0.271
2.373AsnArg: 2.373 ± 0.444
1.998AsnSer: 1.998 ± 0.335
1.561AsnThr: 1.561 ± 0.319
2.435AsnVal: 2.435 ± 0.376
0.874AsnTrp: 0.874 ± 0.229
1.186AsnTyr: 1.186 ± 0.26
0.0AsnXaa: 0.0 ± 0.0
Pro
4.995ProAla: 4.995 ± 0.633
0.5ProCys: 0.5 ± 0.253
3.309ProAsp: 3.309 ± 0.377
4.121ProGlu: 4.121 ± 0.541
1.811ProPhe: 1.811 ± 0.299
5.307ProGly: 5.307 ± 0.702
1.124ProHis: 1.124 ± 0.248
2.185ProIle: 2.185 ± 0.291
2.373ProLys: 2.373 ± 0.477
4.058ProLeu: 4.058 ± 0.511
1.873ProMet: 1.873 ± 0.294
2.31ProAsn: 2.31 ± 0.476
2.498ProPro: 2.498 ± 0.494
2.685ProGln: 2.685 ± 0.64
3.746ProArg: 3.746 ± 0.654
2.685ProSer: 2.685 ± 0.427
4.183ProThr: 4.183 ± 0.64
4.121ProVal: 4.121 ± 0.527
1.311ProTrp: 1.311 ± 0.492
2.06ProTyr: 2.06 ± 0.386
0.0ProXaa: 0.0 ± 0.0
Gln
4.246GlnAla: 4.246 ± 0.48
0.187GlnCys: 0.187 ± 0.123
2.06GlnAsp: 2.06 ± 0.399
1.873GlnGlu: 1.873 ± 0.432
1.436GlnPhe: 1.436 ± 0.305
3.871GlnGly: 3.871 ± 1.029
0.437GlnHis: 0.437 ± 0.15
2.185GlnIle: 2.185 ± 0.415
1.374GlnLys: 1.374 ± 0.471
3.059GlnLeu: 3.059 ± 0.475
0.874GlnMet: 0.874 ± 0.171
1.061GlnAsn: 1.061 ± 0.262
1.561GlnPro: 1.561 ± 0.325
1.686GlnGln: 1.686 ± 0.404
2.373GlnArg: 2.373 ± 0.399
1.811GlnSer: 1.811 ± 0.325
1.623GlnThr: 1.623 ± 0.248
2.622GlnVal: 2.622 ± 0.466
0.687GlnTrp: 0.687 ± 0.226
0.937GlnTyr: 0.937 ± 0.255
0.0GlnXaa: 0.0 ± 0.0
Arg
6.431ArgAla: 6.431 ± 0.748
0.812ArgCys: 0.812 ± 0.279
3.309ArgAsp: 3.309 ± 0.512
5.057ArgGlu: 5.057 ± 0.631
2.56ArgPhe: 2.56 ± 0.463
4.683ArgGly: 4.683 ± 0.605
1.374ArgHis: 1.374 ± 0.279
3.059ArgIle: 3.059 ± 0.39
4.058ArgLys: 4.058 ± 0.575
5.557ArgLeu: 5.557 ± 0.631
2.185ArgMet: 2.185 ± 0.346
2.373ArgAsn: 2.373 ± 0.325
3.247ArgPro: 3.247 ± 0.562
2.123ArgGln: 2.123 ± 0.385
5.245ArgArg: 5.245 ± 0.689
3.621ArgSer: 3.621 ± 0.512
3.184ArgThr: 3.184 ± 0.33
4.995ArgVal: 4.995 ± 0.605
1.561ArgTrp: 1.561 ± 0.34
1.998ArgTyr: 1.998 ± 0.349
0.0ArgXaa: 0.0 ± 0.0
Ser
5.182SerAla: 5.182 ± 0.487
0.062SerCys: 0.062 ± 0.064
3.621SerAsp: 3.621 ± 0.48
3.497SerGlu: 3.497 ± 0.525
2.185SerPhe: 2.185 ± 0.331
5.807SerGly: 5.807 ± 0.866
1.249SerHis: 1.249 ± 0.275
2.498SerIle: 2.498 ± 0.331
2.997SerLys: 2.997 ± 0.499
3.934SerLeu: 3.934 ± 0.537
1.623SerMet: 1.623 ± 0.308
1.686SerAsn: 1.686 ± 0.307
2.747SerPro: 2.747 ± 0.384
1.936SerGln: 1.936 ± 0.242
3.559SerArg: 3.559 ± 0.446
3.184SerSer: 3.184 ± 0.458
2.685SerThr: 2.685 ± 0.36
3.684SerVal: 3.684 ± 0.49
1.061SerTrp: 1.061 ± 0.284
1.186SerTyr: 1.186 ± 0.25
0.0SerXaa: 0.0 ± 0.0
Thr
5.12ThrAla: 5.12 ± 0.577
0.375ThrCys: 0.375 ± 0.142
3.559ThrAsp: 3.559 ± 0.5
3.934ThrGlu: 3.934 ± 0.461
2.373ThrPhe: 2.373 ± 0.4
5.057ThrGly: 5.057 ± 0.49
0.749ThrHis: 0.749 ± 0.225
2.373ThrIle: 2.373 ± 0.398
2.498ThrLys: 2.498 ± 0.444
4.933ThrLeu: 4.933 ± 0.464
1.186ThrMet: 1.186 ± 0.25
0.812ThrAsn: 0.812 ± 0.213
3.871ThrPro: 3.871 ± 0.654
2.248ThrGln: 2.248 ± 0.434
2.81ThrArg: 2.81 ± 0.441
2.997ThrSer: 2.997 ± 0.51
3.559ThrThr: 3.559 ± 0.479
4.246ThrVal: 4.246 ± 0.527
1.061ThrTrp: 1.061 ± 0.283
1.623ThrTyr: 1.623 ± 0.297
0.0ThrXaa: 0.0 ± 0.0
Val
5.557ValAla: 5.557 ± 0.697
0.624ValCys: 0.624 ± 0.244
5.495ValAsp: 5.495 ± 0.64
4.745ValGlu: 4.745 ± 0.584
2.31ValPhe: 2.31 ± 0.382
4.87ValGly: 4.87 ± 0.657
1.124ValHis: 1.124 ± 0.247
3.809ValIle: 3.809 ± 0.48
3.372ValLys: 3.372 ± 0.46
6.306ValLeu: 6.306 ± 0.655
1.186ValMet: 1.186 ± 0.241
3.059ValAsn: 3.059 ± 0.406
4.246ValPro: 4.246 ± 0.522
2.56ValGln: 2.56 ± 0.393
4.496ValArg: 4.496 ± 0.597
4.87ValSer: 4.87 ± 0.522
3.684ValThr: 3.684 ± 0.381
5.182ValVal: 5.182 ± 0.676
1.374ValTrp: 1.374 ± 0.248
2.31ValTyr: 2.31 ± 0.486
0.0ValXaa: 0.0 ± 0.0
Trp
2.123TrpAla: 2.123 ± 0.346
0.375TrpCys: 0.375 ± 0.151
1.374TrpAsp: 1.374 ± 0.326
1.499TrpGlu: 1.499 ± 0.297
0.375TrpPhe: 0.375 ± 0.176
1.436TrpGly: 1.436 ± 0.276
0.5TrpHis: 0.5 ± 0.208
1.374TrpIle: 1.374 ± 0.31
0.624TrpLys: 0.624 ± 0.164
1.748TrpLeu: 1.748 ± 0.362
0.624TrpMet: 0.624 ± 0.201
0.749TrpAsn: 0.749 ± 0.176
0.812TrpPro: 0.812 ± 0.19
0.812TrpGln: 0.812 ± 0.255
0.999TrpArg: 0.999 ± 0.247
1.186TrpSer: 1.186 ± 0.244
1.374TrpThr: 1.374 ± 0.289
1.686TrpVal: 1.686 ± 0.295
0.437TrpTrp: 0.437 ± 0.177
0.5TrpTyr: 0.5 ± 0.16
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.435TyrAla: 2.435 ± 0.388
0.25TyrCys: 0.25 ± 0.12
2.185TyrAsp: 2.185 ± 0.308
1.561TyrGlu: 1.561 ± 0.284
0.749TyrPhe: 0.749 ± 0.173
2.31TyrGly: 2.31 ± 0.371
0.624TyrHis: 0.624 ± 0.245
1.061TyrIle: 1.061 ± 0.264
1.061TyrLys: 1.061 ± 0.27
3.059TyrLeu: 3.059 ± 0.415
0.749TyrMet: 0.749 ± 0.222
1.124TyrAsn: 1.124 ± 0.277
1.311TyrPro: 1.311 ± 0.315
1.061TyrGln: 1.061 ± 0.28
2.872TyrArg: 2.872 ± 0.424
1.436TyrSer: 1.436 ± 0.343
1.561TyrThr: 1.561 ± 0.339
1.936TyrVal: 1.936 ± 0.407
0.687TyrTrp: 0.687 ± 0.24
1.061TyrTyr: 1.061 ± 0.245
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 87 proteins (16017 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski