Pseudonocardia sp. CNS-004

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Pseudonocardia; unclassified Pseudonocardia

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6516 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q9S850|A0A1Q9S850_9PSEU RNA polymerase subunit sigma-24 OS=Pseudonocardia sp. CNS-004 OX=1904967 GN=BJF90_18495 PE=3 SV=1
MM1 pKa = 7.87AILNGGPNDD10 pKa = 4.21EE11 pKa = 4.35IVGPARR17 pKa = 11.84QEE19 pKa = 3.8GFLAGLTEE27 pKa = 4.57GGVTPEE33 pKa = 4.18VVASATATYY42 pKa = 8.05TQEE45 pKa = 3.93EE46 pKa = 4.72AVPATEE52 pKa = 4.73SMLAAHH58 pKa = 7.41PEE60 pKa = 3.94TDD62 pKa = 4.65LVLGLNDD69 pKa = 3.74SMALGALSVLRR80 pKa = 11.84NQGNSTVLVAAAADD94 pKa = 4.07GQKK97 pKa = 10.4EE98 pKa = 4.06ALAEE102 pKa = 4.03IANGGCTGQYY112 pKa = 10.74VSTGLNSPSLAAEE125 pKa = 3.91QVFDD129 pKa = 3.71IAVGVATGQLDD140 pKa = 3.62PATVQKK146 pKa = 10.06TSLTEE151 pKa = 3.56AAGIGCEE158 pKa = 4.19NVAEE162 pKa = 4.36YY163 pKa = 10.7YY164 pKa = 10.63DD165 pKa = 3.98PSSIFF170 pKa = 3.18

Molecular weight: 17.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q9S294|A0A1Q9S294_9PSEU RNA polymerase sigma factor SigE OS=Pseudonocardia sp. CNS-004 OX=1904967 GN=BJF90_03480 PE=3 SV=1
MM1 pKa = 7.53SKK3 pKa = 10.53GKK5 pKa = 8.66RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84THH19 pKa = 5.69GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVAARR37 pKa = 11.84RR38 pKa = 11.84GKK40 pKa = 10.12GRR42 pKa = 11.84QRR44 pKa = 11.84LSAA47 pKa = 4.04

Molecular weight: 5.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6516

0

6516

1658514

29

2552

254.5

27.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.124 ± 0.054

0.842 ± 0.01

5.954 ± 0.031

5.321 ± 0.029

2.721 ± 0.021

9.488 ± 0.033

2.236 ± 0.016

3.296 ± 0.021

1.254 ± 0.015

10.322 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.796 ± 0.014

1.537 ± 0.014

6.26 ± 0.03

2.555 ± 0.017

8.678 ± 0.038

5.074 ± 0.031

5.887 ± 0.023

9.402 ± 0.036

1.514 ± 0.013

1.739 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski