Amino acid dipepetide frequency for Pseudonocardia sp. CNS-004

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	23.785AlaAla: 23.785 ± 0.196	1.197AlaCys: 1.197 ± 0.03	8.442AlaAsp: 8.442 ± 0.076	8.333AlaGlu: 8.333 ± 0.081	3.676AlaPhe: 3.676 ± 0.05	14.389AlaGly: 14.389 ± 0.106	2.816AlaHis: 2.816 ± 0.045	4.273AlaIle: 4.273 ± 0.058	1.703AlaLys: 1.703 ± 0.032	14.574AlaLeu: 14.574 ± 0.122	2.762AlaMet: 2.762 ± 0.039	1.774AlaAsn: 1.774 ± 0.033	7.24AlaPro: 7.24 ± 0.075	3.578AlaGln: 3.578 ± 0.052	11.101AlaArg: 11.101 ± 0.102	6.028AlaSer: 6.028 ± 0.08	7.744AlaThr: 7.744 ± 0.077	13.865AlaVal: 13.865 ± 0.107	1.867AlaTrp: 1.867 ± 0.038	2.098AlaTyr: 2.098 ± 0.045	0.0AlaXaa: 0.0 ± 0.0
Cys	1.121CysAla: 1.121 ± 0.028	0.173CysCys: 0.173 ± 0.011	0.485CysAsp: 0.485 ± 0.02	0.374CysGlu: 0.374 ± 0.017	0.186CysPhe: 0.186 ± 0.009	1.042CysGly: 1.042 ± 0.024	0.186CysHis: 0.186 ± 0.01	0.207CysIle: 0.207 ± 0.011	0.053CysLys: 0.053 ± 0.006	0.651CysLeu: 0.651 ± 0.02	0.138CysMet: 0.138 ± 0.01	0.111CysAsn: 0.111 ± 0.008	0.534CysPro: 0.534 ± 0.02	0.142CysGln: 0.142 ± 0.01	0.787CysArg: 0.787 ± 0.025	0.67CysSer: 0.67 ± 0.023	0.604CysThr: 0.604 ± 0.019	0.64CysVal: 0.64 ± 0.02	0.18CysTrp: 0.18 ± 0.011	0.14CysTyr: 0.14 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	8.077AspAla: 8.077 ± 0.082	0.341AspCys: 0.341 ± 0.017	3.959AspAsp: 3.959 ± 0.078	3.955AspGlu: 3.955 ± 0.056	1.313AspPhe: 1.313 ± 0.032	6.316AspGly: 6.316 ± 0.067	1.432AspHis: 1.432 ± 0.028	1.653AspIle: 1.653 ± 0.033	0.658AspLys: 0.658 ± 0.024	6.256AspLeu: 6.256 ± 0.062	0.754AspMet: 0.754 ± 0.022	0.805AspAsn: 0.805 ± 0.024	4.683AspPro: 4.683 ± 0.05	1.425AspGln: 1.425 ± 0.033	5.425AspArg: 5.425 ± 0.066	2.079AspSer: 2.079 ± 0.043	2.795AspThr: 2.795 ± 0.045	5.858AspVal: 5.858 ± 0.06	0.788AspTrp: 0.788 ± 0.023	0.968AspTyr: 0.968 ± 0.023	0.0AspXaa: 0.0 ± 0.0
Glu	6.406GluAla: 6.406 ± 0.069	0.33GluCys: 0.33 ± 0.017	2.338GluAsp: 2.338 ± 0.045	2.771GluGlu: 2.771 ± 0.046	1.578GluPhe: 1.578 ± 0.035	3.424GluGly: 3.424 ± 0.05	1.648GluHis: 1.648 ± 0.033	2.294GluIle: 2.294 ± 0.041	0.919GluLys: 0.919 ± 0.031	7.097GluLeu: 7.097 ± 0.063	0.858GluMet: 0.858 ± 0.023	0.918GluAsn: 0.918 ± 0.028	3.556GluPro: 3.556 ± 0.054	2.257GluGln: 2.257 ± 0.041	5.672GluArg: 5.672 ± 0.064	2.385GluSer: 2.385 ± 0.045	2.34GluThr: 2.34 ± 0.039	4.678GluVal: 4.678 ± 0.063	0.818GluTrp: 0.818 ± 0.021	0.925GluTyr: 0.925 ± 0.023	0.0GluXaa: 0.0 ± 0.0
Phe	3.919PheAla: 3.919 ± 0.058	0.254PheCys: 0.254 ± 0.011	1.966PheAsp: 1.966 ± 0.036	1.351PheGlu: 1.351 ± 0.03	0.86PhePhe: 0.86 ± 0.027	3.144PheGly: 3.144 ± 0.052	0.585PheHis: 0.585 ± 0.019	0.721PheIle: 0.721 ± 0.025	0.253PheLys: 0.253 ± 0.013	2.668PheLeu: 2.668 ± 0.044	0.362PheMet: 0.362 ± 0.015	0.45PheAsn: 0.45 ± 0.017	1.399PhePro: 1.399 ± 0.031	0.581PheGln: 0.581 ± 0.019	1.864PheArg: 1.864 ± 0.035	1.408PheSer: 1.408 ± 0.029	1.812PheThr: 1.812 ± 0.032	2.623PheVal: 2.623 ± 0.048	0.453PheTrp: 0.453 ± 0.02	0.537PheTyr: 0.537 ± 0.025	0.0PheXaa: 0.0 ± 0.0
Gly	11.408GlyAla: 11.408 ± 0.102	0.98GlyCys: 0.98 ± 0.026	4.945GlyAsp: 4.945 ± 0.062	5.078GlyGlu: 5.078 ± 0.065	2.92GlyPhe: 2.92 ± 0.048	8.904GlyGly: 8.904 ± 0.098	2.198GlyHis: 2.198 ± 0.041	3.708GlyIle: 3.708 ± 0.051	1.492GlyLys: 1.492 ± 0.031	9.744GlyLeu: 9.744 ± 0.1	2.218GlyMet: 2.218 ± 0.041	1.501GlyAsn: 1.501 ± 0.034	5.574GlyPro: 5.574 ± 0.068	2.332GlyGln: 2.332 ± 0.044	8.476GlyArg: 8.476 ± 0.088	5.493GlySer: 5.493 ± 0.067	5.648GlyThr: 5.648 ± 0.062	8.305GlyVal: 8.305 ± 0.073	1.831GlyTrp: 1.831 ± 0.037	2.125GlyTyr: 2.125 ± 0.036	0.0GlyXaa: 0.0 ± 0.0
His	2.703HisAla: 2.703 ± 0.043	0.21HisCys: 0.21 ± 0.012	1.486HisAsp: 1.486 ± 0.032	1.17HisGlu: 1.17 ± 0.026	0.55HisPhe: 0.55 ± 0.02	2.381HisGly: 2.381 ± 0.042	0.69HisHis: 0.69 ± 0.021	0.555HisIle: 0.555 ± 0.02	0.207HisLys: 0.207 ± 0.01	2.394HisLeu: 2.394 ± 0.043	0.306HisMet: 0.306 ± 0.015	0.335HisAsn: 0.335 ± 0.014	1.764HisPro: 1.764 ± 0.034	0.564HisGln: 0.564 ± 0.019	2.306HisArg: 2.306 ± 0.043	0.924HisSer: 0.924 ± 0.024	1.127HisThr: 1.127 ± 0.03	1.958HisVal: 1.958 ± 0.038	0.303HisTrp: 0.303 ± 0.013	0.43HisTyr: 0.43 ± 0.017	0.0HisXaa: 0.0 ± 0.0
Ile	5.472IleAla: 5.472 ± 0.065	0.291IleCys: 0.291 ± 0.015	2.405IleAsp: 2.405 ± 0.043	1.966IleGlu: 1.966 ± 0.034	0.76IlePhe: 0.76 ± 0.022	3.926IleGly: 3.926 ± 0.05	0.571IleHis: 0.571 ± 0.02	0.878IleIle: 0.878 ± 0.028	0.411IleLys: 0.411 ± 0.018	2.216IleLeu: 2.216 ± 0.039	0.458IleMet: 0.458 ± 0.017	0.641IleAsn: 0.641 ± 0.02	1.797IlePro: 1.797 ± 0.034	0.587IleGln: 0.587 ± 0.017	2.356IleArg: 2.356 ± 0.042	1.696IleSer: 1.696 ± 0.035	2.268IleThr: 2.268 ± 0.041	3.326IleVal: 3.326 ± 0.05	0.406IleTrp: 0.406 ± 0.016	0.528IleTyr: 0.528 ± 0.02	0.0IleXaa: 0.0 ± 0.0
Lys	1.686LysAla: 1.686 ± 0.039	0.058LysCys: 0.058 ± 0.007	0.662LysAsp: 0.662 ± 0.026	0.635LysGlu: 0.635 ± 0.023	0.3LysPhe: 0.3 ± 0.014	0.995LysGly: 0.995 ± 0.028	0.297LysHis: 0.297 ± 0.015	0.608LysIle: 0.608 ± 0.022	0.321LysLys: 0.321 ± 0.029	1.243LysLeu: 1.243 ± 0.032	0.242LysMet: 0.242 ± 0.014	0.262LysAsn: 0.262 ± 0.014	0.794LysPro: 0.794 ± 0.024	0.436LysGln: 0.436 ± 0.018	1.043LysArg: 1.043 ± 0.028	0.685LysSer: 0.685 ± 0.029	0.678LysThr: 0.678 ± 0.02	1.184LysVal: 1.184 ± 0.026	0.171LysTrp: 0.171 ± 0.012	0.236LysTyr: 0.236 ± 0.012	0.0LysXaa: 0.0 ± 0.0
Leu	16.028LeuAla: 16.028 ± 0.121	0.682LeuCys: 0.682 ± 0.021	6.946LeuAsp: 6.946 ± 0.066	4.574LeuGlu: 4.574 ± 0.067	2.635LeuPhe: 2.635 ± 0.049	9.622LeuGly: 9.622 ± 0.093	2.295LeuHis: 2.295 ± 0.04	3.073LeuIle: 3.073 ± 0.045	0.994LeuLys: 0.994 ± 0.024	11.425LeuLeu: 11.425 ± 0.124	1.476LeuMet: 1.476 ± 0.032	1.358LeuAsn: 1.358 ± 0.029	6.262LeuPro: 6.262 ± 0.069	2.41LeuGln: 2.41 ± 0.044	8.868LeuArg: 8.868 ± 0.086	4.918LeuSer: 4.918 ± 0.056	6.231LeuThr: 6.231 ± 0.064	10.698LeuVal: 10.698 ± 0.115	1.21LeuTrp: 1.21 ± 0.03	1.514LeuTyr: 1.514 ± 0.032	0.0LeuXaa: 0.0 ± 0.0
Met	2.306MetAla: 2.306 ± 0.038	0.128MetCys: 0.128 ± 0.008	0.771MetAsp: 0.771 ± 0.022	0.657MetGlu: 0.657 ± 0.019	0.485MetPhe: 0.485 ± 0.019	1.323MetGly: 1.323 ± 0.029	0.385MetHis: 0.385 ± 0.014	0.819MetIle: 0.819 ± 0.021	0.296MetLys: 0.296 ± 0.013	1.932MetLeu: 1.932 ± 0.034	0.285MetMet: 0.285 ± 0.016	0.397MetAsn: 0.397 ± 0.018	1.216MetPro: 1.216 ± 0.026	0.478MetGln: 0.478 ± 0.018	1.617MetArg: 1.617 ± 0.032	1.32MetSer: 1.32 ± 0.029	1.616MetThr: 1.616 ± 0.031	1.44MetVal: 1.44 ± 0.031	0.232MetTrp: 0.232 ± 0.012	0.258MetTyr: 0.258 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	1.99AsnAla: 1.99 ± 0.038	0.116AsnCys: 0.116 ± 0.008	0.769AsnAsp: 0.769 ± 0.024	0.733AsnGlu: 0.733 ± 0.021	0.447AsnPhe: 0.447 ± 0.018	1.562AsnGly: 1.562 ± 0.038	0.327AsnHis: 0.327 ± 0.014	0.549AsnIle: 0.549 ± 0.02	0.198AsnLys: 0.198 ± 0.013	1.535AsnLeu: 1.535 ± 0.029	0.232AsnMet: 0.232 ± 0.012	0.34AsnAsn: 0.34 ± 0.016	1.247AsnPro: 1.247 ± 0.028	0.433AsnGln: 0.433 ± 0.018	1.271AsnArg: 1.271 ± 0.024	0.78AsnSer: 0.78 ± 0.025	0.916AsnThr: 0.916 ± 0.025	1.356AsnVal: 1.356 ± 0.032	0.242AsnTrp: 0.242 ± 0.013	0.324AsnTyr: 0.324 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	9.138ProAla: 9.138 ± 0.091	0.441ProCys: 0.441 ± 0.016	4.815ProAsp: 4.815 ± 0.058	3.742ProGlu: 3.742 ± 0.049	1.609ProPhe: 1.609 ± 0.033	6.733ProGly: 6.733 ± 0.077	1.351ProHis: 1.351 ± 0.03	1.685ProIle: 1.685 ± 0.036	0.678ProLys: 0.678 ± 0.019	5.203ProLeu: 5.203 ± 0.059	1.141ProMet: 1.141 ± 0.026	0.848ProAsn: 0.848 ± 0.025	4.23ProPro: 4.23 ± 0.08	1.582ProGln: 1.582 ± 0.036	4.625ProArg: 4.625 ± 0.063	3.255ProSer: 3.255 ± 0.051	3.759ProThr: 3.759 ± 0.05	5.824ProVal: 5.824 ± 0.061	0.895ProTrp: 0.895 ± 0.026	1.041ProTyr: 1.041 ± 0.026	0.0ProXaa: 0.0 ± 0.0
Gln	3.404GlnAla: 3.404 ± 0.05	0.165GlnCys: 0.165 ± 0.01	1.224GlnAsp: 1.224 ± 0.029	1.278GlnGlu: 1.278 ± 0.027	0.705GlnPhe: 0.705 ± 0.021	1.785GlnGly: 1.785 ± 0.037	0.626GlnHis: 0.626 ± 0.021	1.015GlnIle: 1.015 ± 0.027	0.388GlnLys: 0.388 ± 0.017	3.181GlnLeu: 3.181 ± 0.048	0.48GlnMet: 0.48 ± 0.017	0.476GlnAsn: 0.476 ± 0.017	1.612GlnPro: 1.612 ± 0.036	1.17GlnGln: 1.17 ± 0.035	2.517GlnArg: 2.517 ± 0.038	1.029GlnSer: 1.029 ± 0.025	1.126GlnThr: 1.126 ± 0.027	2.516GlnVal: 2.516 ± 0.04	0.365GlnTrp: 0.365 ± 0.016	0.483GlnTyr: 0.483 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	11.024ArgAla: 11.024 ± 0.101	0.921ArgCys: 0.921 ± 0.028	4.561ArgAsp: 4.561 ± 0.061	4.63ArgGlu: 4.63 ± 0.065	2.554ArgPhe: 2.554 ± 0.043	6.572ArgGly: 6.572 ± 0.076	2.156ArgHis: 2.156 ± 0.039	3.32ArgIle: 3.32 ± 0.048	1.168ArgLys: 1.168 ± 0.032	8.626ArgLeu: 8.626 ± 0.085	2.081ArgMet: 2.081 ± 0.034	1.356ArgAsn: 1.356 ± 0.03	5.736ArgPro: 5.736 ± 0.072	2.091ArgGln: 2.091 ± 0.033	9.457ArgArg: 9.457 ± 0.125	5.232ArgSer: 5.232 ± 0.072	5.311ArgThr: 5.311 ± 0.063	6.59ArgVal: 6.59 ± 0.062	1.653ArgTrp: 1.653 ± 0.032	1.746ArgTyr: 1.746 ± 0.033	0.0ArgXaa: 0.0 ± 0.0
Ser	6.877SerAla: 6.877 ± 0.077	0.554SerCys: 0.554 ± 0.02	2.691SerAsp: 2.691 ± 0.054	2.192SerGlu: 2.192 ± 0.051	1.408SerPhe: 1.408 ± 0.03	5.723SerGly: 5.723 ± 0.071	0.878SerHis: 0.878 ± 0.022	1.664SerIle: 1.664 ± 0.032	0.636SerLys: 0.636 ± 0.02	4.19SerLeu: 4.19 ± 0.053	1.246SerMet: 1.246 ± 0.028	0.803SerAsn: 0.803 ± 0.024	3.434SerPro: 3.434 ± 0.053	1.068SerGln: 1.068 ± 0.027	4.318SerArg: 4.318 ± 0.054	3.388SerSer: 3.388 ± 0.07	3.474SerThr: 3.474 ± 0.05	4.281SerVal: 4.281 ± 0.053	0.979SerTrp: 0.979 ± 0.024	0.938SerTyr: 0.938 ± 0.022	0.0SerXaa: 0.0 ± 0.0
Thr	8.553ThrAla: 8.553 ± 0.079	0.518ThrCys: 0.518 ± 0.019	3.329ThrAsp: 3.329 ± 0.044	2.832ThrGlu: 2.832 ± 0.038	1.638ThrPhe: 1.638 ± 0.033	6.288ThrGly: 6.288 ± 0.073	1.094ThrHis: 1.094 ± 0.027	2.049ThrIle: 2.049 ± 0.036	0.73ThrLys: 0.73 ± 0.022	5.325ThrLeu: 5.325 ± 0.054	1.037ThrMet: 1.037 ± 0.028	0.882ThrAsn: 0.882 ± 0.022	3.929ThrPro: 3.929 ± 0.05	1.164ThrGln: 1.164 ± 0.029	4.253ThrArg: 4.253 ± 0.056	3.327ThrSer: 3.327 ± 0.05	3.904ThrThr: 3.904 ± 0.051	6.018ThrVal: 6.018 ± 0.067	0.953ThrTrp: 0.953 ± 0.027	1.043ThrTyr: 1.043 ± 0.022	0.0ThrXaa: 0.0 ± 0.0
Val	13.394ValAla: 13.394 ± 0.109	0.707ValCys: 0.707 ± 0.021	5.887ValAsp: 5.887 ± 0.072	5.319ValGlu: 5.319 ± 0.053	2.483ValPhe: 2.483 ± 0.045	7.893ValGly: 7.893 ± 0.085	2.138ValHis: 2.138 ± 0.04	2.935ValIle: 2.935 ± 0.052	1.017ValLys: 1.017 ± 0.031	11.217ValLeu: 11.217 ± 0.11	1.332ValMet: 1.332 ± 0.029	1.472ValAsn: 1.472 ± 0.035	5.732ValPro: 5.732 ± 0.066	2.251ValGln: 2.251 ± 0.034	7.674ValArg: 7.674 ± 0.064	4.299ValSer: 4.299 ± 0.057	5.582ValThr: 5.582 ± 0.058	10.191ValVal: 10.191 ± 0.104	1.129ValTrp: 1.129 ± 0.026	1.375ValTyr: 1.375 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	1.734TrpAla: 1.734 ± 0.029	0.193TrpCys: 0.193 ± 0.011	0.759TrpAsp: 0.759 ± 0.025	0.674TrpGlu: 0.674 ± 0.023	0.525TrpPhe: 0.525 ± 0.017	1.067TrpGly: 1.067 ± 0.027	0.353TrpHis: 0.353 ± 0.015	0.535TrpIle: 0.535 ± 0.017	0.229TrpLys: 0.229 ± 0.011	1.768TrpLeu: 1.768 ± 0.038	0.344TrpMet: 0.344 ± 0.016	0.33TrpAsn: 0.33 ± 0.016	0.913TrpPro: 0.913 ± 0.027	0.504TrpGln: 0.504 ± 0.019	1.456TrpArg: 1.456 ± 0.032	0.957TrpSer: 0.957 ± 0.024	0.967TrpThr: 0.967 ± 0.025	1.164TrpVal: 1.164 ± 0.028	0.376TrpTrp: 0.376 ± 0.016	0.292TrpTyr: 0.292 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.221TyrAla: 2.221 ± 0.039	0.162TyrCys: 0.162 ± 0.01	1.099TyrAsp: 1.099 ± 0.026	0.948TyrGlu: 0.948 ± 0.026	0.577TyrPhe: 0.577 ± 0.017	1.788TyrGly: 1.788 ± 0.038	0.338TyrHis: 0.338 ± 0.015	0.42TyrIle: 0.42 ± 0.017	0.186TyrLys: 0.186 ± 0.011	1.973TyrLeu: 1.973 ± 0.038	0.21TyrMet: 0.21 ± 0.012	0.307TyrAsn: 0.307 ± 0.015	0.944TyrPro: 0.944 ± 0.025	0.496TyrGln: 0.496 ± 0.015	1.701TyrArg: 1.701 ± 0.032	0.866TyrSer: 0.866 ± 0.023	0.966TyrThr: 0.966 ± 0.028	1.51TyrVal: 1.51 ± 0.03	0.29TyrTrp: 0.29 ± 0.014	0.393TyrTyr: 0.393 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6516 proteins (1658514 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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