Common bottlenose dolphin gammaherpesvirus 1 strain Sarasota
Average proteome isoelectric point is 7.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Z1NE30|A0A1Z1NE30_9GAMA DNA replication helicase OS=Common bottlenose dolphin gammaherpesvirus 1 strain Sarasota OX=2022783 GN=ORF44 PE=3 SV=1
MM1 pKa = 8.0 AMFLLRR7 pKa = 11.84 EE8 pKa = 4.3 AKK10 pKa = 9.36 VARR13 pKa = 11.84 GRR15 pKa = 11.84 RR16 pKa = 11.84 RR17 pKa = 11.84 PDD19 pKa = 3.2 DD20 pKa = 4.05 RR21 pKa = 11.84 MLPIYY26 pKa = 8.97 GAPRR30 pKa = 11.84 RR31 pKa = 11.84 KK32 pKa = 5.9 TTEE35 pKa = 3.65 YY36 pKa = 9.32 FTFPTHH42 pKa = 7.32 LSSLDD47 pKa = 3.8 LSDD50 pKa = 4.71 SSSLSPSSPLTEE62 pKa = 4.54 DD63 pKa = 4.68 RR64 pKa = 11.84 EE65 pKa = 4.55 LCSTAVTSSTLNASVGEE82 pKa = 4.09 NGPCRR87 pKa = 11.84 LRR89 pKa = 11.84 LRR91 pKa = 11.84 NLKK94 pKa = 9.51 TSEE97 pKa = 3.86 AALKK101 pKa = 10.53 GGRR104 pKa = 11.84 CSMGCGKK111 pKa = 10.11 LAYY114 pKa = 9.97 QRR116 pKa = 11.84 IRR118 pKa = 11.84 PLNSKK123 pKa = 9.63 IDD125 pKa = 3.7 EE126 pKa = 4.58 DD127 pKa = 4.47 SEE129 pKa = 4.69 CEE131 pKa = 3.93 EE132 pKa = 5.25 GEE134 pKa = 4.49 GCGGNAAASALRR146 pKa = 11.84 DD147 pKa = 3.74 DD148 pKa = 4.67 VKK150 pKa = 11.3 DD151 pKa = 3.76 EE152 pKa = 4.15 EE153 pKa = 5.89 GEE155 pKa = 4.87 DD156 pKa = 4.08 GDD158 pKa = 4.88 CVEE161 pKa = 5.79 GEE163 pKa = 5.0 DD164 pKa = 5.07 GDD166 pKa = 4.98 EE167 pKa = 4.43 SDD169 pKa = 4.25 SDD171 pKa = 4.39 DD172 pKa = 4.08 YY173 pKa = 12.07 EE174 pKa = 4.27 EE175 pKa = 6.01 DD176 pKa = 3.78 EE177 pKa = 4.45 EE178 pKa = 5.59 DD179 pKa = 4.53 EE180 pKa = 4.13 EE181 pKa = 5.63 DD182 pKa = 4.67 EE183 pKa = 4.24 EE184 pKa = 5.63 DD185 pKa = 4.67 EE186 pKa = 4.6 EE187 pKa = 5.63 DD188 pKa = 5.23 EE189 pKa = 4.3 EE190 pKa = 6.34 DD191 pKa = 3.56 EE192 pKa = 5.2 DD193 pKa = 4.63 EE194 pKa = 4.43 EE195 pKa = 5.33 GEE197 pKa = 4.12 EE198 pKa = 4.52 GEE200 pKa = 4.42 EE201 pKa = 4.74 DD202 pKa = 4.81 EE203 pKa = 5.37 EE204 pKa = 6.36 DD205 pKa = 3.72 EE206 pKa = 5.76 DD207 pKa = 5.63 DD208 pKa = 5.28 EE209 pKa = 4.89 EE210 pKa = 6.49 DD211 pKa = 3.97 EE212 pKa = 4.59 EE213 pKa = 6.1 SDD215 pKa = 5.54 DD216 pKa = 5.38 GSEE219 pKa = 4.17 LCPGDD224 pKa = 3.7 SRR226 pKa = 11.84 YY227 pKa = 10.64 EE228 pKa = 4.01 DD229 pKa = 3.61 VSDD232 pKa = 4.55 EE233 pKa = 4.55 DD234 pKa = 4.69 EE235 pKa = 4.38 SDD237 pKa = 3.94 CFIDD241 pKa = 4.71 EE242 pKa = 4.1 ADD244 pKa = 3.57 KK245 pKa = 11.48 VNEE248 pKa = 3.83 IRR250 pKa = 11.84 VADD253 pKa = 4.11 EE254 pKa = 5.64 DD255 pKa = 3.75 IGSSDD260 pKa = 3.86 TEE262 pKa = 4.56 SEE264 pKa = 4.39 CSSDD268 pKa = 4.72 DD269 pKa = 3.69 NSPCSSVSRR278 pKa = 11.84 NGDD281 pKa = 3.31 EE282 pKa = 4.34 GDD284 pKa = 3.93 VIVISSDD291 pKa = 4.04 DD292 pKa = 3.65 EE293 pKa = 4.84 GEE295 pKa = 4.18 SCLRR299 pKa = 11.84 KK300 pKa = 9.62 AAGDD304 pKa = 3.7 GLEE307 pKa = 5.12 GSDD310 pKa = 5.19 SDD312 pKa = 4.47 DD313 pKa = 3.87 DD314 pKa = 4.23 CVLVSSTYY322 pKa = 10.37 KK323 pKa = 10.26 RR324 pKa = 11.84 RR325 pKa = 11.84 LSSSSEE331 pKa = 3.79 PQTPDD336 pKa = 2.66 ILKK339 pKa = 9.97 KK340 pKa = 7.67 SARR343 pKa = 11.84 MIVYY347 pKa = 10.07 SSSSSDD353 pKa = 3.59 EE354 pKa = 4.13 EE355 pKa = 5.13 DD356 pKa = 3.74 DD357 pKa = 4.73 SGTGSQSSVDD367 pKa = 3.58 GSRR370 pKa = 11.84 EE371 pKa = 3.78 TGTSYY376 pKa = 11.34 RR377 pKa = 11.84 MPTSYY382 pKa = 11.03 LPTRR386 pKa = 11.84 PRR388 pKa = 11.84 SCKK391 pKa = 9.86 KK392 pKa = 9.92 SSNRR396 pKa = 11.84 YY397 pKa = 7.31 SRR399 pKa = 11.84 RR400 pKa = 11.84 VMHH403 pKa = 6.27 KK404 pKa = 7.91 TPPLKK409 pKa = 10.89 GHH411 pKa = 7.33 DD412 pKa = 3.98 CYY414 pKa = 11.5 NWPWLDD420 pKa = 3.21
Molecular weight: 46.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.203
IPC2_protein 3.973
IPC_protein 3.973
Toseland 3.77
ProMoST 4.088
Dawson 3.935
Bjellqvist 4.101
Wikipedia 3.834
Rodwell 3.795
Grimsley 3.681
Solomon 3.935
Lehninger 3.897
Nozaki 4.05
DTASelect 4.24
Thurlkill 3.795
EMBOSS 3.846
Sillero 4.088
Patrickios 3.528
IPC_peptide 3.935
IPC2_peptide 4.075
IPC2.peptide.svr19 4.014
Protein with the highest isoelectric point:
>tr|A0A1Z1NEG6|A0A1Z1NEG6_9GAMA Shutoff alkaline exonuclease OS=Common bottlenose dolphin gammaherpesvirus 1 strain Sarasota OX=2022783 GN=ORF37 PE=3 SV=1
MM1 pKa = 7.48 SGGVIPGLGDD11 pKa = 3.39 ANAVLGTLPARR22 pKa = 11.84 CKK24 pKa = 10.36 AAGKK28 pKa = 9.42 KK29 pKa = 9.82 AHH31 pKa = 6.39 LKK33 pKa = 10.3 VYY35 pKa = 10.5 RR36 pKa = 11.84 KK37 pKa = 10.09 LLGFKK42 pKa = 7.87 TLRR45 pKa = 11.84 GLSLFLDD52 pKa = 4.17 LGQPVEE58 pKa = 4.63 SSAKK62 pKa = 9.26 HH63 pKa = 5.65 RR64 pKa = 11.84 IFFEE68 pKa = 3.88 VRR70 pKa = 11.84 LGRR73 pKa = 11.84 RR74 pKa = 11.84 IADD77 pKa = 3.38 CVLIVKK83 pKa = 9.12 TEE85 pKa = 3.87 DD86 pKa = 3.03 RR87 pKa = 11.84 GVCYY91 pKa = 10.07 IIEE94 pKa = 5.1 LKK96 pKa = 9.68 TCMTQNVNIFSPIRR110 pKa = 11.84 QAQRR114 pKa = 11.84 LQGLAQLSDD123 pKa = 3.49 SVKK126 pKa = 10.21 FLRR129 pKa = 11.84 DD130 pKa = 3.55 ALPSGPEE137 pKa = 3.47 SWFIIPVLLFKK148 pKa = 11.02 SQGSFKK154 pKa = 9.62 TLHH157 pKa = 6.12 SEE159 pKa = 5.06 TYY161 pKa = 9.52 QSSSQGARR169 pKa = 11.84 TSWEE173 pKa = 3.72 RR174 pKa = 11.84 LTNFLFVRR182 pKa = 11.84 EE183 pKa = 4.21 DD184 pKa = 3.71 DD185 pKa = 4.1 AVTGALRR192 pKa = 11.84 QNRR195 pKa = 11.84 RR196 pKa = 11.84 PRR198 pKa = 11.84 VVPDD202 pKa = 3.58 RR203 pKa = 11.84 NLLGPQVVKK212 pKa = 10.18 RR213 pKa = 11.84 HH214 pKa = 4.09 VRR216 pKa = 11.84 KK217 pKa = 9.71 RR218 pKa = 11.84 RR219 pKa = 11.84 PPQRR223 pKa = 11.84 GDD225 pKa = 3.2 APGAPTARR233 pKa = 11.84 TQRR236 pKa = 11.84 GPRR239 pKa = 11.84 GTGGDD244 pKa = 4.19 CEE246 pKa = 5.5 LSVQGAAPAGHH257 pKa = 7.19 DD258 pKa = 3.7 EE259 pKa = 4.23 ARR261 pKa = 11.84 GVQQ264 pKa = 3.31
Molecular weight: 29.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.339
IPC2_protein 9.545
IPC_protein 10.16
Toseland 10.789
ProMoST 10.438
Dawson 10.847
Bjellqvist 10.54
Wikipedia 11.038
Rodwell 11.067
Grimsley 10.877
Solomon 10.994
Lehninger 10.965
Nozaki 10.774
DTASelect 10.526
Thurlkill 10.774
EMBOSS 11.184
Sillero 10.789
Patrickios 10.789
IPC_peptide 10.994
IPC2_peptide 9.604
IPC2.peptide.svr19 8.687
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
72
0
72
35681
83
2739
495.6
55.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.144 ± 0.249
2.416 ± 0.186
4.997 ± 0.168
5.073 ± 0.211
4.568 ± 0.188
5.762 ± 0.201
3.105 ± 0.113
4.975 ± 0.185
4.515 ± 0.184
9.938 ± 0.224
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.071 ± 0.104
4.235 ± 0.142
7.135 ± 0.606
3.43 ± 0.128
5.518 ± 0.163
7.533 ± 0.189
5.908 ± 0.198
6.474 ± 0.236
1.076 ± 0.064
3.125 ± 0.147
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here