Clostridium algidicarnis DSM 15099
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2618 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S6FVT8|A0A2S6FVT8_9CLOT Lipopolysaccharide cholinephosphotransferase OS=Clostridium algidicarnis DSM 15099 OX=1121295 GN=BD821_11646 PE=4 SV=1
MM1 pKa = 7.72 EE2 pKa = 5.38 ISKK5 pKa = 10.46 EE6 pKa = 3.88 MTIGDD11 pKa = 3.69 VVMEE15 pKa = 4.18 YY16 pKa = 10.3 PEE18 pKa = 4.26 SVEE21 pKa = 3.9 VLMSFGMGCVGCPASQMEE39 pKa = 4.51 TLEE42 pKa = 4.13 EE43 pKa = 3.64 AAMVHH48 pKa = 6.13 GFDD51 pKa = 4.57 IKK53 pKa = 11.06 EE54 pKa = 4.16 LLSALNGG61 pKa = 3.67
Molecular weight: 6.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.859
IPC2_protein 4.101
IPC_protein 3.884
Toseland 3.757
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.706
Rodwell 3.732
Grimsley 3.681
Solomon 3.795
Lehninger 3.745
Nozaki 3.973
DTASelect 3.999
Thurlkill 3.783
EMBOSS 3.719
Sillero 3.999
Patrickios 1.863
IPC_peptide 3.808
IPC2_peptide 3.986
IPC2.peptide.svr19 3.901
Protein with the highest isoelectric point:
>tr|A0A2S6G1E5|A0A2S6G1E5_9CLOT Cell division protein FtsI/penicillin-binding protein 2 OS=Clostridium algidicarnis DSM 15099 OX=1121295 GN=BD821_101283 PE=4 SV=1
MM1 pKa = 7.53 SRR3 pKa = 11.84 RR4 pKa = 11.84 CEE6 pKa = 4.0 YY7 pKa = 10.33 CDD9 pKa = 3.4 KK10 pKa = 11.28 GVMSGVQYY18 pKa = 10.81 SHH20 pKa = 6.33 SHH22 pKa = 5.36 RR23 pKa = 11.84 QSKK26 pKa = 10.2 RR27 pKa = 11.84 KK28 pKa = 7.68 WAPNIKK34 pKa = 9.68 KK35 pKa = 9.98 IKK37 pKa = 10.06 AIVNGTPRR45 pKa = 11.84 TVHH48 pKa = 4.66 VCTRR52 pKa = 11.84 CLRR55 pKa = 11.84 SGKK58 pKa = 7.94 VEE60 pKa = 3.89 RR61 pKa = 11.84 AII63 pKa = 4.51
Molecular weight: 7.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.335
IPC2_protein 9.706
IPC_protein 10.204
Toseland 10.76
ProMoST 10.35
Dawson 10.833
Bjellqvist 10.511
Wikipedia 10.994
Rodwell 11.213
Grimsley 10.877
Solomon 10.935
Lehninger 10.906
Nozaki 10.76
DTASelect 10.496
Thurlkill 10.745
EMBOSS 11.155
Sillero 10.774
Patrickios 10.979
IPC_peptide 10.935
IPC2_peptide 9.692
IPC2.peptide.svr19 8.507
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2618
0
2618
808886
29
2948
309.0
34.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.383 ± 0.047
1.063 ± 0.018
5.758 ± 0.035
7.606 ± 0.058
4.261 ± 0.035
6.441 ± 0.045
1.331 ± 0.017
10.192 ± 0.055
9.419 ± 0.048
9.129 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.734 ± 0.02
6.033 ± 0.038
2.832 ± 0.025
2.15 ± 0.024
3.33 ± 0.033
6.569 ± 0.039
4.754 ± 0.032
6.351 ± 0.039
0.606 ± 0.016
4.057 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here