Ectocarpus siliculosus virus 1 (isolate New Zealand/Kaikoura/1988) (EsV-1)

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Algavirales; Phycodnaviridae; Phaeovirus; Ectocarpus siliculosus virus 1

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 231 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8QKV5|Q8QKV5_ESV1K EsV-1-97 OS=Ectocarpus siliculosus virus 1 (isolate New Zealand/Kaikoura/1988) OX=654926 GN=ORF 97 PE=4 SV=1
MM1 pKa = 7.74FSILYY6 pKa = 6.07NTNMLGLAKK15 pKa = 10.34SILLLLTSGLHH26 pKa = 6.44CGHH29 pKa = 7.41ASLTVTTPEE38 pKa = 3.64EE39 pKa = 4.07DD40 pKa = 3.4LVVVSGRR47 pKa = 11.84TFTVRR52 pKa = 11.84WNQDD56 pKa = 3.42DD57 pKa = 4.05GSTTYY62 pKa = 11.12SRR64 pKa = 11.84FAIDD68 pKa = 4.19LYY70 pKa = 10.84QYY72 pKa = 10.89QSYY75 pKa = 11.53AMDD78 pKa = 4.28EE79 pKa = 4.38DD80 pKa = 4.7SCGSWVTALCPYY92 pKa = 10.14GEE94 pKa = 4.49RR95 pKa = 11.84GCPDD99 pKa = 2.74STGDD103 pKa = 3.59YY104 pKa = 10.94DD105 pKa = 4.41VVLPEE110 pKa = 4.79PATDD114 pKa = 5.02LPDD117 pKa = 3.05STYY120 pKa = 10.58RR121 pKa = 11.84IGVLGVEE128 pKa = 4.97DD129 pKa = 4.81GSFGCSDD136 pKa = 3.75KK137 pKa = 10.65FTLTTEE143 pKa = 4.01QATSKK148 pKa = 11.28DD149 pKa = 3.16PDD151 pKa = 3.51KK152 pKa = 11.5YY153 pKa = 10.36FLSVTAPADD162 pKa = 3.23GDD164 pKa = 3.95VAVIGDD170 pKa = 3.85TYY172 pKa = 10.98EE173 pKa = 3.96VQFSYY178 pKa = 11.4EE179 pKa = 4.02NGVGSSADD187 pKa = 3.35VFDD190 pKa = 4.96IDD192 pKa = 6.15LFTSDD197 pKa = 3.94CGTYY201 pKa = 10.36VAGLCDD207 pKa = 3.31QCKK210 pKa = 10.51DD211 pKa = 3.44SGGSHH216 pKa = 7.62PIIIPMDD223 pKa = 3.63TDD225 pKa = 3.47ADD227 pKa = 4.54FYY229 pKa = 10.47RR230 pKa = 11.84IRR232 pKa = 11.84VARR235 pKa = 11.84VEE237 pKa = 4.39DD238 pKa = 4.27PFLFDD243 pKa = 3.33CSGVFEE249 pKa = 4.55VVDD252 pKa = 3.29ILSFSFWW259 pKa = 3.86

Molecular weight: 28.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8QNH8|Q8QNH8_ESV1K EsV-1-100 OS=Ectocarpus siliculosus virus 1 (isolate New Zealand/Kaikoura/1988) OX=654926 GN=ORF 100 PE=4 SV=1
MM1 pKa = 7.67HH2 pKa = 7.13SMKK5 pKa = 10.18ILKK8 pKa = 9.71NDD10 pKa = 2.72RR11 pKa = 11.84RR12 pKa = 11.84YY13 pKa = 10.21FLARR17 pKa = 11.84DD18 pKa = 3.35VLQRR22 pKa = 11.84ILHH25 pKa = 5.49KK26 pKa = 10.96VPLLFDD32 pKa = 3.55PRR34 pKa = 11.84GRR36 pKa = 11.84WRR38 pKa = 11.84CRR40 pKa = 11.84HH41 pKa = 5.54FEE43 pKa = 4.0ATFSCIRR50 pKa = 11.84LPTPLLPGACLRR62 pKa = 11.84LQRR65 pKa = 11.84RR66 pKa = 11.84TRR68 pKa = 11.84PLLVALDD75 pKa = 4.49NIRR78 pKa = 11.84QQLVEE83 pKa = 4.12VSTLGEE89 pKa = 3.89ILVVGVTRR97 pKa = 11.84PYY99 pKa = 10.58HH100 pKa = 5.22VLHH103 pKa = 6.76EE104 pKa = 4.66GSEE107 pKa = 4.22EE108 pKa = 4.14VLWVVAIVPVASVVRR123 pKa = 11.84QKK125 pKa = 11.54KK126 pKa = 9.96LVDD129 pKa = 3.27

Molecular weight: 14.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

231

0

231

73957

53

1233

320.2

35.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.625 ± 0.195

2.092 ± 0.094

6.145 ± 0.132

5.906 ± 0.203

4.09 ± 0.116

6.527 ± 0.174

2.488 ± 0.076

5.064 ± 0.115

5.89 ± 0.277

8.152 ± 0.152

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.779 ± 0.068

4.093 ± 0.105

4.104 ± 0.145

3.244 ± 0.125

6.147 ± 0.173

8.155 ± 0.195

6.2 ± 0.144

7.519 ± 0.126

0.972 ± 0.044

2.81 ± 0.096
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski