Pseudomonas phage OBP

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Petsuvirus; unclassified Petsuvirus

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 309 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G9I9Y0|G9I9Y0_9CAUD HNH nuclease OS=Pseudomonas phage OBP OX=1124849 GN=OBP_088 PE=4 SV=1
MM1 pKa = 7.88PLNNTAGTLPATEE14 pKa = 4.11QAMVTYY20 pKa = 10.41KK21 pKa = 10.21FKK23 pKa = 11.3CPDD26 pKa = 3.17LAEE29 pKa = 4.4FFEE32 pKa = 4.67TLNPEE37 pKa = 3.96QSHH40 pKa = 6.21IALEE44 pKa = 4.34GALNIIFAKK53 pKa = 10.41PPMSLEE59 pKa = 4.24DD60 pKa = 3.34VMAPMGDD67 pKa = 3.33GTEE70 pKa = 4.73APDD73 pKa = 3.72EE74 pKa = 4.61DD75 pKa = 5.18EE76 pKa = 4.99IEE78 pKa = 4.42PPQSEE83 pKa = 4.94EE84 pKa = 3.96EE85 pKa = 4.15CQMDD89 pKa = 3.5HH90 pKa = 6.74EE91 pKa = 5.19GSVTQATVLALFDD104 pKa = 3.79HH105 pKa = 5.86QQGILAEE112 pKa = 4.26AAGITDD118 pKa = 4.44MVPVDD123 pKa = 4.4DD124 pKa = 5.33PEE126 pKa = 4.26NSPLYY131 pKa = 10.26QQMEE135 pKa = 4.74EE136 pKa = 3.76IFGNIVTKK144 pKa = 10.73LSSDD148 pKa = 2.83ITEE151 pKa = 4.33DD152 pKa = 3.72RR153 pKa = 11.84FNQDD157 pKa = 2.35VITLYY162 pKa = 11.5GEE164 pKa = 4.02MEE166 pKa = 4.25FFSRR170 pKa = 11.84IEE172 pKa = 3.92MFTYY176 pKa = 10.3QYY178 pKa = 8.73NTKK181 pKa = 8.9TDD183 pKa = 3.54QLIVTISACNAA194 pKa = 3.46

Molecular weight: 21.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G9I9T2|G9I9T2_9CAUD Uncharacterized protein OS=Pseudomonas phage OBP OX=1124849 GN=OBP_040 PE=4 SV=1
MM1 pKa = 6.97QRR3 pKa = 11.84TRR5 pKa = 11.84AYY7 pKa = 10.11RR8 pKa = 11.84RR9 pKa = 11.84YY10 pKa = 8.69KK11 pKa = 10.66LQVKK15 pKa = 8.91KK16 pKa = 11.16VKK18 pKa = 10.29VSRR21 pKa = 11.84YY22 pKa = 5.97WNAGVWRR29 pKa = 11.84IGGCQRR35 pKa = 11.84QTDD38 pKa = 3.72KK39 pKa = 11.68AVIGRR44 pKa = 11.84VANTPKK50 pKa = 10.86ACGCWMCANARR61 pKa = 11.84QVFGEE66 pKa = 4.03PFADD70 pKa = 3.48VRR72 pKa = 11.84RR73 pKa = 11.84KK74 pKa = 9.16QLYY77 pKa = 9.48VDD79 pKa = 3.79QEE81 pKa = 4.12

Molecular weight: 9.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

309

0

309

89744

39

4079

290.4

32.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.387 ± 0.157

0.789 ± 0.046

6.583 ± 0.087

6.264 ± 0.125

4.456 ± 0.104

6.784 ± 0.165

1.881 ± 0.095

6.753 ± 0.118

6.657 ± 0.116

8.278 ± 0.151

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.811 ± 0.108

5.495 ± 0.103

4.187 ± 0.123

3.175 ± 0.077

4.418 ± 0.135

6.105 ± 0.096

6.575 ± 0.258

7.28 ± 0.194

1.219 ± 0.058

3.903 ± 0.09
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski