Amino acid dipepetide frequency for Pseudomonas phage OBP

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.647AlaAla: 4.647 ± 0.402
0.435AlaCys: 0.435 ± 0.067
4.167AlaAsp: 4.167 ± 0.213
3.922AlaGlu: 3.922 ± 0.224
2.752AlaPhe: 2.752 ± 0.205
4.814AlaGly: 4.814 ± 0.304
1.103AlaHis: 1.103 ± 0.121
4.379AlaIle: 4.379 ± 0.23
4.167AlaLys: 4.167 ± 0.245
5.527AlaLeu: 5.527 ± 0.246
1.761AlaMet: 1.761 ± 0.176
3.577AlaAsn: 3.577 ± 0.244
2.385AlaPro: 2.385 ± 0.174
2.162AlaGln: 2.162 ± 0.172
2.663AlaArg: 2.663 ± 0.173
3.61AlaSer: 3.61 ± 0.276
4.268AlaThr: 4.268 ± 0.251
4.892AlaVal: 4.892 ± 0.262
0.791AlaTrp: 0.791 ± 0.089
1.85AlaTyr: 1.85 ± 0.14
0.0AlaXaa: 0.0 ± 0.0
Cys
0.357CysAla: 0.357 ± 0.055
0.056CysCys: 0.056 ± 0.023
0.524CysAsp: 0.524 ± 0.072
0.457CysGlu: 0.457 ± 0.085
0.412CysPhe: 0.412 ± 0.074
0.646CysGly: 0.646 ± 0.098
0.145CysHis: 0.145 ± 0.036
0.423CysIle: 0.423 ± 0.072
0.501CysLys: 0.501 ± 0.077
0.602CysLeu: 0.602 ± 0.083
0.167CysMet: 0.167 ± 0.04
0.435CysAsn: 0.435 ± 0.069
0.357CysPro: 0.357 ± 0.058
0.245CysGln: 0.245 ± 0.05
0.435CysArg: 0.435 ± 0.073
0.501CysSer: 0.501 ± 0.07
0.446CysThr: 0.446 ± 0.068
0.646CysVal: 0.646 ± 0.089
0.167CysTrp: 0.167 ± 0.045
0.368CysTyr: 0.368 ± 0.073
0.0CysXaa: 0.0 ± 0.0
Asp
3.711AspAla: 3.711 ± 0.233
0.479AspCys: 0.479 ± 0.077
4.179AspAsp: 4.179 ± 0.226
4.011AspGlu: 4.011 ± 0.241
3.343AspPhe: 3.343 ± 0.208
4.747AspGly: 4.747 ± 0.242
1.281AspHis: 1.281 ± 0.125
5.226AspIle: 5.226 ± 0.265
4.401AspLys: 4.401 ± 0.24
5.817AspLeu: 5.817 ± 0.23
1.839AspMet: 1.839 ± 0.161
3.165AspAsn: 3.165 ± 0.17
3.332AspPro: 3.332 ± 0.208
2.05AspGln: 2.05 ± 0.145
2.518AspArg: 2.518 ± 0.179
3.231AspSer: 3.231 ± 0.186
3.677AspThr: 3.677 ± 0.233
4.914AspVal: 4.914 ± 0.277
0.969AspTrp: 0.969 ± 0.097
2.942AspTyr: 2.942 ± 0.208
0.0AspXaa: 0.0 ± 0.0
Glu
4.39GluAla: 4.39 ± 0.306
0.535GluCys: 0.535 ± 0.072
3.61GluAsp: 3.61 ± 0.209
4.413GluGlu: 4.413 ± 0.287
2.797GluPhe: 2.797 ± 0.187
4.234GluGly: 4.234 ± 0.238
1.27GluHis: 1.27 ± 0.13
4.468GluIle: 4.468 ± 0.254
3.499GluLys: 3.499 ± 0.237
6.585GluLeu: 6.585 ± 0.318
1.939GluMet: 1.939 ± 0.164
2.797GluAsn: 2.797 ± 0.19
2.039GluPro: 2.039 ± 0.166
1.961GluGln: 1.961 ± 0.183
2.719GluArg: 2.719 ± 0.207
3.555GluSer: 3.555 ± 0.192
3.421GluThr: 3.421 ± 0.208
5.07GluVal: 5.07 ± 0.314
0.735GluTrp: 0.735 ± 0.086
2.607GluTyr: 2.607 ± 0.163
0.0GluXaa: 0.0 ± 0.0
Phe
2.574PheAla: 2.574 ± 0.17
0.312PheCys: 0.312 ± 0.066
3.41PheAsp: 3.41 ± 0.251
2.63PheGlu: 2.63 ± 0.196
1.883PhePhe: 1.883 ± 0.192
3.343PheGly: 3.343 ± 0.167
0.791PheHis: 0.791 ± 0.098
2.953PheIle: 2.953 ± 0.233
3.867PheLys: 3.867 ± 0.211
3.02PheLeu: 3.02 ± 0.191
1.17PheMet: 1.17 ± 0.115
3.176PheAsn: 3.176 ± 0.253
1.593PhePro: 1.593 ± 0.128
1.059PheGln: 1.059 ± 0.114
1.983PheArg: 1.983 ± 0.141
2.908PheSer: 2.908 ± 0.221
3.12PheThr: 3.12 ± 0.223
2.507PheVal: 2.507 ± 0.175
0.546PheTrp: 0.546 ± 0.077
1.716PheTyr: 1.716 ± 0.15
0.0PheXaa: 0.0 ± 0.0
Gly
3.922GlyAla: 3.922 ± 0.332
0.646GlyCys: 0.646 ± 0.096
4.379GlyAsp: 4.379 ± 0.231
4.089GlyGlu: 4.089 ± 0.237
3.454GlyPhe: 3.454 ± 0.208
4.479GlyGly: 4.479 ± 0.349
1.025GlyHis: 1.025 ± 0.116
4.491GlyIle: 4.491 ± 0.209
5.46GlyLys: 5.46 ± 0.284
5.349GlyLeu: 5.349 ± 0.267
2.195GlyMet: 2.195 ± 0.216
4.212GlyAsn: 4.212 ± 0.212
1.783GlyPro: 1.783 ± 0.153
1.928GlyGln: 1.928 ± 0.162
2.763GlyArg: 2.763 ± 0.161
4.19GlySer: 4.19 ± 0.223
4.58GlyThr: 4.58 ± 0.286
4.914GlyVal: 4.914 ± 0.264
0.936GlyTrp: 0.936 ± 0.115
3.042GlyTyr: 3.042 ± 0.178
0.0GlyXaa: 0.0 ± 0.0
His
1.259HisAla: 1.259 ± 0.106
0.223HisCys: 0.223 ± 0.058
1.003HisAsp: 1.003 ± 0.115
1.159HisGlu: 1.159 ± 0.128
1.047HisPhe: 1.047 ± 0.099
0.969HisGly: 0.969 ± 0.115
0.557HisHis: 0.557 ± 0.082
1.159HisIle: 1.159 ± 0.134
1.237HisLys: 1.237 ± 0.118
1.816HisLeu: 1.816 ± 0.164
0.379HisMet: 0.379 ± 0.068
0.78HisAsn: 0.78 ± 0.095
1.237HisPro: 1.237 ± 0.121
0.691HisGln: 0.691 ± 0.076
0.914HisArg: 0.914 ± 0.113
0.836HisSer: 0.836 ± 0.091
1.17HisThr: 1.17 ± 0.121
1.17HisVal: 1.17 ± 0.134
0.223HisTrp: 0.223 ± 0.058
0.981HisTyr: 0.981 ± 0.123
0.0HisXaa: 0.0 ± 0.0
Ile
4.401IleAla: 4.401 ± 0.228
0.501IleCys: 0.501 ± 0.079
5.337IleAsp: 5.337 ± 0.312
4.624IleGlu: 4.624 ± 0.29
2.24IlePhe: 2.24 ± 0.181
3.945IleGly: 3.945 ± 0.21
1.504IleHis: 1.504 ± 0.155
4.123IleIle: 4.123 ± 0.249
5.137IleLys: 5.137 ± 0.251
4.624IleLeu: 4.624 ± 0.249
1.538IleMet: 1.538 ± 0.15
3.989IleAsn: 3.989 ± 0.239
3.243IlePro: 3.243 ± 0.217
2.073IleGln: 2.073 ± 0.143
3.421IleArg: 3.421 ± 0.181
4.725IleSer: 4.725 ± 0.242
5.126IleThr: 5.126 ± 0.374
3.789IleVal: 3.789 ± 0.218
0.747IleTrp: 0.747 ± 0.077
2.429IleTyr: 2.429 ± 0.163
0.0IleXaa: 0.0 ± 0.0
Lys
4.535LysAla: 4.535 ± 0.233
0.379LysCys: 0.379 ± 0.063
4.903LysAsp: 4.903 ± 0.26
4.903LysGlu: 4.903 ± 0.289
2.663LysPhe: 2.663 ± 0.196
4.29LysGly: 4.29 ± 0.287
1.593LysHis: 1.593 ± 0.14
3.933LysIle: 3.933 ± 0.209
3.844LysLys: 3.844 ± 0.213
6.084LysLeu: 6.084 ± 0.253
2.206LysMet: 2.206 ± 0.146
2.808LysAsn: 2.808 ± 0.189
2.652LysPro: 2.652 ± 0.184
2.396LysGln: 2.396 ± 0.138
2.897LysArg: 2.897 ± 0.201
3.766LysSer: 3.766 ± 0.225
3.945LysThr: 3.945 ± 0.194
5.282LysVal: 5.282 ± 0.276
0.802LysTrp: 0.802 ± 0.083
2.685LysTyr: 2.685 ± 0.175
0.0LysXaa: 0.0 ± 0.0
Leu
5.415LeuAla: 5.415 ± 0.286
0.535LeuCys: 0.535 ± 0.08
5.906LeuAsp: 5.906 ± 0.263
4.569LeuGlu: 4.569 ± 0.261
3.276LeuPhe: 3.276 ± 0.227
5.037LeuGly: 5.037 ± 0.224
1.404LeuHis: 1.404 ± 0.145
5.393LeuIle: 5.393 ± 0.265
6.229LeuLys: 6.229 ± 0.279
6.262LeuLeu: 6.262 ± 0.295
2.396LeuMet: 2.396 ± 0.179
5.438LeuAsn: 5.438 ± 0.221
3.354LeuPro: 3.354 ± 0.189
2.418LeuGln: 2.418 ± 0.171
3.922LeuArg: 3.922 ± 0.228
5.46LeuSer: 5.46 ± 0.268
6.374LeuThr: 6.374 ± 0.355
5.995LeuVal: 5.995 ± 0.296
0.669LeuTrp: 0.669 ± 0.079
2.73LeuTyr: 2.73 ± 0.163
0.0LeuXaa: 0.0 ± 0.0
Met
2.039MetAla: 2.039 ± 0.152
0.234MetCys: 0.234 ± 0.047
1.961MetAsp: 1.961 ± 0.159
1.961MetGlu: 1.961 ± 0.146
1.694MetPhe: 1.694 ± 0.206
2.006MetGly: 2.006 ± 0.196
0.279MetHis: 0.279 ± 0.062
1.449MetIle: 1.449 ± 0.132
1.917MetLys: 1.917 ± 0.145
2.162MetLeu: 2.162 ± 0.178
0.903MetMet: 0.903 ± 0.133
1.571MetAsn: 1.571 ± 0.134
1.036MetPro: 1.036 ± 0.107
0.914MetGln: 0.914 ± 0.097
0.914MetArg: 0.914 ± 0.1
2.284MetSer: 2.284 ± 0.185
1.705MetThr: 1.705 ± 0.13
1.738MetVal: 1.738 ± 0.138
0.345MetTrp: 0.345 ± 0.063
1.003MetTyr: 1.003 ± 0.102
0.0MetXaa: 0.0 ± 0.0
Asn
3.477AsnAla: 3.477 ± 0.225
0.446AsnCys: 0.446 ± 0.061
2.841AsnAsp: 2.841 ± 0.198
3.287AsnGlu: 3.287 ± 0.233
2.451AsnPhe: 2.451 ± 0.161
3.922AsnGly: 3.922 ± 0.243
0.869AsnHis: 0.869 ± 0.099
3.61AsnIle: 3.61 ± 0.236
3.777AsnLys: 3.777 ± 0.22
4.323AsnLeu: 4.323 ± 0.23
1.348AsnMet: 1.348 ± 0.142
3.087AsnAsn: 3.087 ± 0.218
3.165AsnPro: 3.165 ± 0.176
1.995AsnGln: 1.995 ± 0.151
2.697AsnArg: 2.697 ± 0.168
3.276AsnSer: 3.276 ± 0.195
3.566AsnThr: 3.566 ± 0.221
3.933AsnVal: 3.933 ± 0.221
0.758AsnTrp: 0.758 ± 0.114
2.117AsnTyr: 2.117 ± 0.144
0.0AsnXaa: 0.0 ± 0.0
Pro
2.819ProAla: 2.819 ± 0.241
0.201ProCys: 0.201 ± 0.036
2.563ProAsp: 2.563 ± 0.156
3.343ProGlu: 3.343 ± 0.183
1.839ProPhe: 1.839 ± 0.15
3.087ProGly: 3.087 ± 0.201
0.702ProHis: 0.702 ± 0.078
3.187ProIle: 3.187 ± 0.213
2.529ProLys: 2.529 ± 0.163
3.131ProLeu: 3.131 ± 0.176
0.981ProMet: 0.981 ± 0.134
2.385ProAsn: 2.385 ± 0.191
1.248ProPro: 1.248 ± 0.142
1.103ProGln: 1.103 ± 0.114
1.393ProArg: 1.393 ± 0.145
2.763ProSer: 2.763 ± 0.173
2.953ProThr: 2.953 ± 0.191
3.722ProVal: 3.722 ± 0.219
0.39ProTrp: 0.39 ± 0.061
1.538ProTyr: 1.538 ± 0.153
0.0ProXaa: 0.0 ± 0.0
Gln
2.295GlnAla: 2.295 ± 0.148
0.379GlnCys: 0.379 ± 0.071
1.705GlnAsp: 1.705 ± 0.155
1.504GlnGlu: 1.504 ± 0.129
1.616GlnPhe: 1.616 ± 0.127
1.827GlnGly: 1.827 ± 0.135
0.557GlnHis: 0.557 ± 0.079
2.229GlnIle: 2.229 ± 0.14
1.56GlnLys: 1.56 ± 0.143
3.243GlnLeu: 3.243 ± 0.207
0.969GlnMet: 0.969 ± 0.102
1.46GlnAsn: 1.46 ± 0.114
1.304GlnPro: 1.304 ± 0.113
1.293GlnGln: 1.293 ± 0.145
1.761GlnArg: 1.761 ± 0.13
1.861GlnSer: 1.861 ± 0.174
1.794GlnThr: 1.794 ± 0.142
2.641GlnVal: 2.641 ± 0.185
0.423GlnTrp: 0.423 ± 0.083
1.326GlnTyr: 1.326 ± 0.132
0.0GlnXaa: 0.0 ± 0.0
Arg
2.853ArgAla: 2.853 ± 0.171
0.401ArgCys: 0.401 ± 0.064
2.797ArgAsp: 2.797 ± 0.181
2.641ArgGlu: 2.641 ± 0.199
2.128ArgPhe: 2.128 ± 0.158
2.752ArgGly: 2.752 ± 0.202
1.059ArgHis: 1.059 ± 0.127
3.231ArgIle: 3.231 ± 0.219
2.819ArgLys: 2.819 ± 0.19
4.179ArgLeu: 4.179 ± 0.263
1.493ArgMet: 1.493 ± 0.148
2.34ArgAsn: 2.34 ± 0.157
1.649ArgPro: 1.649 ± 0.112
1.426ArgGln: 1.426 ± 0.143
1.983ArgArg: 1.983 ± 0.151
2.162ArgSer: 2.162 ± 0.145
2.752ArgThr: 2.752 ± 0.21
2.919ArgVal: 2.919 ± 0.196
0.591ArgTrp: 0.591 ± 0.088
2.006ArgTyr: 2.006 ± 0.18
0.0ArgXaa: 0.0 ± 0.0
Ser
3.421SerAla: 3.421 ± 0.203
0.49SerCys: 0.49 ± 0.08
3.967SerAsp: 3.967 ± 0.218
3.488SerGlu: 3.488 ± 0.223
2.853SerPhe: 2.853 ± 0.21
4.791SerGly: 4.791 ± 0.303
1.159SerHis: 1.159 ± 0.116
4.39SerIle: 4.39 ± 0.229
3.933SerLys: 3.933 ± 0.23
5.048SerLeu: 5.048 ± 0.259
1.995SerMet: 1.995 ± 0.165
2.953SerAsn: 2.953 ± 0.211
2.407SerPro: 2.407 ± 0.154
2.006SerGln: 2.006 ± 0.157
2.886SerArg: 2.886 ± 0.201
3.933SerSer: 3.933 ± 0.303
3.8SerThr: 3.8 ± 0.236
4.557SerVal: 4.557 ± 0.238
0.724SerTrp: 0.724 ± 0.092
2.251SerTyr: 2.251 ± 0.168
0.0SerXaa: 0.0 ± 0.0
Thr
4.145ThrAla: 4.145 ± 0.285
0.334ThrCys: 0.334 ± 0.059
4.201ThrAsp: 4.201 ± 0.246
3.555ThrGlu: 3.555 ± 0.189
3.332ThrPhe: 3.332 ± 0.267
4.992ThrGly: 4.992 ± 0.354
1.315ThrHis: 1.315 ± 0.115
4.702ThrIle: 4.702 ± 0.258
3.454ThrLys: 3.454 ± 0.192
5.661ThrLeu: 5.661 ± 0.311
1.56ThrMet: 1.56 ± 0.122
3.187ThrAsn: 3.187 ± 0.206
3.644ThrPro: 3.644 ± 0.259
1.972ThrGln: 1.972 ± 0.15
2.931ThrArg: 2.931 ± 0.164
3.945ThrSer: 3.945 ± 0.274
4.0ThrThr: 4.0 ± 0.279
5.081ThrVal: 5.081 ± 0.345
0.947ThrTrp: 0.947 ± 0.122
2.797ThrTyr: 2.797 ± 0.236
0.0ThrXaa: 0.0 ± 0.0
Val
4.591ValAla: 4.591 ± 0.254
0.735ValCys: 0.735 ± 0.103
5.003ValAsp: 5.003 ± 0.228
5.148ValGlu: 5.148 ± 0.32
2.641ValPhe: 2.641 ± 0.214
4.368ValGly: 4.368 ± 0.229
1.125ValHis: 1.125 ± 0.121
4.903ValIle: 4.903 ± 0.215
5.081ValLys: 5.081 ± 0.275
5.282ValLeu: 5.282 ± 0.275
1.939ValMet: 1.939 ± 0.141
4.156ValAsn: 4.156 ± 0.224
3.12ValPro: 3.12 ± 0.204
2.284ValGln: 2.284 ± 0.164
3.009ValArg: 3.009 ± 0.175
4.803ValSer: 4.803 ± 0.225
5.727ValThr: 5.727 ± 0.443
5.616ValVal: 5.616 ± 0.393
0.802ValTrp: 0.802 ± 0.094
2.463ValTyr: 2.463 ± 0.165
0.0ValXaa: 0.0 ± 0.0
Trp
0.713TrpAla: 0.713 ± 0.083
0.156TrpCys: 0.156 ± 0.044
0.969TrpAsp: 0.969 ± 0.129
0.702TrpGlu: 0.702 ± 0.064
0.557TrpPhe: 0.557 ± 0.079
0.825TrpGly: 0.825 ± 0.113
0.245TrpHis: 0.245 ± 0.06
0.903TrpIle: 0.903 ± 0.106
0.669TrpLys: 0.669 ± 0.079
0.891TrpLeu: 0.891 ± 0.111
0.357TrpMet: 0.357 ± 0.053
0.914TrpAsn: 0.914 ± 0.14
0.379TrpPro: 0.379 ± 0.064
0.39TrpGln: 0.39 ± 0.071
0.535TrpArg: 0.535 ± 0.093
0.691TrpSer: 0.691 ± 0.082
0.702TrpThr: 0.702 ± 0.085
0.858TrpVal: 0.858 ± 0.094
0.189TrpTrp: 0.189 ± 0.043
0.546TrpTyr: 0.546 ± 0.082
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.307TyrAla: 2.307 ± 0.148
0.412TyrCys: 0.412 ± 0.069
2.407TyrAsp: 2.407 ± 0.173
2.24TyrGlu: 2.24 ± 0.163
1.582TyrPhe: 1.582 ± 0.129
2.763TyrGly: 2.763 ± 0.175
0.825TyrHis: 0.825 ± 0.091
2.373TyrIle: 2.373 ± 0.189
2.485TyrLys: 2.485 ± 0.184
3.176TyrLeu: 3.176 ± 0.223
0.969TyrMet: 0.969 ± 0.099
2.518TyrAsn: 2.518 ± 0.169
1.95TyrPro: 1.95 ± 0.123
1.382TyrGln: 1.382 ± 0.115
1.839TyrArg: 1.839 ± 0.161
2.552TyrSer: 2.552 ± 0.182
2.63TyrThr: 2.63 ± 0.221
2.552TyrVal: 2.552 ± 0.167
0.435TyrTrp: 0.435 ± 0.067
1.638TyrTyr: 1.638 ± 0.15
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 309 proteins (89744 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski