Paraglaciecola psychrophila 170

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; Paraglaciecola; Paraglaciecola psychrophila

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5601 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K7AW16|K7AW16_9ALTE Shikimate kinase OS=Paraglaciecola psychrophila 170 OX=1129794 GN=aroK PE=3 SV=1
MM1 pKa = 7.76NSFLKK6 pKa = 10.18TGITSALFFICGFAVAAKK24 pKa = 10.21SPLIPYY30 pKa = 10.16SKK32 pKa = 9.47TNSQIAEE39 pKa = 3.92TRR41 pKa = 11.84IVGGEE46 pKa = 3.77EE47 pKa = 4.0AIQEE51 pKa = 3.93NWPWMTAYY59 pKa = 10.65VGVFTNFLTSLSVNNVIYY77 pKa = 7.37EE78 pKa = 4.3TRR80 pKa = 11.84SFTSGVGGQVSGEE93 pKa = 4.05IIACGIGDD101 pKa = 4.77AVCVDD106 pKa = 3.36ATGNVCLIEE115 pKa = 4.49RR116 pKa = 11.84GIVDD120 pKa = 4.44FSEE123 pKa = 5.48KK124 pKa = 10.45SDD126 pKa = 3.32NCEE129 pKa = 3.66AGGGIGAIIYY139 pKa = 10.6NNEE142 pKa = 3.77EE143 pKa = 3.55VDD145 pKa = 4.0NITGGTLGEE154 pKa = 5.32DD155 pKa = 3.64YY156 pKa = 10.51TGTIPIVAVNQKK168 pKa = 10.8DD169 pKa = 3.66GLALLEE175 pKa = 4.13QIGNIASLAVSEE187 pKa = 4.44TTEE190 pKa = 4.05LQQDD194 pKa = 4.26ASCGASFLGDD204 pKa = 3.1KK205 pKa = 9.65WVLTAAHH212 pKa = 6.55CVDD215 pKa = 4.84SEE217 pKa = 4.22NANQFKK223 pKa = 10.53MNVGEE228 pKa = 4.12YY229 pKa = 10.17DD230 pKa = 3.52LSNGAEE236 pKa = 4.02NAIDD240 pKa = 3.3IANIYY245 pKa = 9.67IHH247 pKa = 6.39PQYY250 pKa = 11.06DD251 pKa = 3.19ADD253 pKa = 5.33AISNDD258 pKa = 2.86IALIEE263 pKa = 4.32LASSSDD269 pKa = 3.41AVGVKK274 pKa = 9.85IADD277 pKa = 3.84PDD279 pKa = 3.72VTDD282 pKa = 3.7QYY284 pKa = 11.78AIEE287 pKa = 4.41NSLATVAGWGGRR299 pKa = 11.84AGYY302 pKa = 10.07APNEE306 pKa = 4.42GPTSDD311 pKa = 5.08FPDD314 pKa = 3.72ILHH317 pKa = 6.64KK318 pKa = 10.69VDD320 pKa = 5.98LRR322 pKa = 11.84LTTNAQCRR330 pKa = 11.84EE331 pKa = 4.01EE332 pKa = 4.39LGEE335 pKa = 4.19SFGISAANVGVTDD348 pKa = 4.09VMICAAIPEE357 pKa = 4.62GGRR360 pKa = 11.84GSCQGDD366 pKa = 3.06SGGPLVVNTGSGVQQVGIVSWGFGCAASGYY396 pKa = 8.09PGVYY400 pKa = 9.17TRR402 pKa = 11.84VSEE405 pKa = 4.35FKK407 pKa = 10.51DD408 pKa = 3.4WISAITDD415 pKa = 4.82GIAITQRR422 pKa = 11.84HH423 pKa = 5.09NFGLGLEE430 pKa = 4.84GEE432 pKa = 4.59VQTTGLAVTNNSEE445 pKa = 4.54TNVGLSFAFSDD456 pKa = 4.2SNTFTLDD463 pKa = 3.11ASNCPTLDD471 pKa = 3.7AGNSCQVSVSYY482 pKa = 11.01LPTIANNVSSKK493 pKa = 11.08LIITTDD499 pKa = 3.52DD500 pKa = 3.76PQVQSSSATVTAGTTLGYY518 pKa = 10.43AVEE521 pKa = 4.45LAATAGSEE529 pKa = 4.12SDD531 pKa = 3.46AVTLFSGGTNGASAWVASSTEE552 pKa = 3.92VGIEE556 pKa = 3.95STTTGDD562 pKa = 3.52LQDD565 pKa = 4.53SIFVASIEE573 pKa = 4.33GEE575 pKa = 4.36GVLTFDD581 pKa = 3.53WAVSSEE587 pKa = 3.86ANTLVEE593 pKa = 4.15TDD595 pKa = 3.42PDD597 pKa = 3.91FEE599 pKa = 5.36RR600 pKa = 11.84FDD602 pKa = 4.86ALFLYY607 pKa = 10.98LNGDD611 pKa = 3.71LVDD614 pKa = 4.66VINGNDD620 pKa = 3.59DD621 pKa = 4.02EE622 pKa = 5.38ISFTEE627 pKa = 3.95YY628 pKa = 11.11SLNLSEE634 pKa = 6.52GINLVNWTYY643 pKa = 11.61SKK645 pKa = 11.3DD646 pKa = 3.64PDD648 pKa = 3.86VSEE651 pKa = 5.27GDD653 pKa = 3.34DD654 pKa = 3.2KK655 pKa = 11.92GFIRR659 pKa = 11.84NLVFTATVVAPPPVAVVPIPAPPNSSGGAGSLGWILLCLVGLIFRR704 pKa = 11.84LRR706 pKa = 11.84TKK708 pKa = 10.45HH709 pKa = 5.98

Molecular weight: 73.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K7ALB5|K7ALB5_9ALTE Uncharacterized protein OS=Paraglaciecola psychrophila 170 OX=1129794 GN=C427_2761 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.14RR12 pKa = 11.84KK13 pKa = 9.13RR14 pKa = 11.84SHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.38NGRR28 pKa = 11.84KK29 pKa = 9.48VIANRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.35GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5601

0

5601

1496535

37

3094

267.2

29.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.108 ± 0.036

1.026 ± 0.012

5.608 ± 0.035

5.711 ± 0.037

4.4 ± 0.028

6.632 ± 0.042

2.207 ± 0.019

6.878 ± 0.026

5.629 ± 0.035

10.344 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.568 ± 0.021

4.759 ± 0.03

3.75 ± 0.023

4.761 ± 0.042

4.11 ± 0.028

6.957 ± 0.032

5.474 ± 0.037

6.701 ± 0.032

1.236 ± 0.012

3.139 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski