Fox fecal rhabdovirus 
Average proteome isoelectric point is 6.64 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|A0A060D558|A0A060D558_9RHAB Alpha 1 protein OS=Fox fecal rhabdovirus OX=1504569 PE=4 SV=1MM1 pKa = 7.47  SPQHH5 pKa = 6.15  GASALISGVDD15 pKa = 3.49  PDD17 pKa = 4.47  HH18 pKa = 6.95  FSLIIEE24 pKa = 4.65  SYY26 pKa = 9.84  PILVGNYY33 pKa = 8.63  AATLILTVTLVLSFSLATIIFFFSSVNNLTDD64 pKa = 2.92  IRR66 pKa = 11.84  GVLIYY71 pKa = 10.6  HH72 pKa = 7.24  LGNEE76 pKa = 4.04  VSEE79 pKa = 4.67  FASHH83 pKa = 7.11  ALAAVCSAQSTSARR97 pKa = 11.84  LTRR100 pKa = 11.84  EE101 pKa = 3.85  LEE103 pKa = 3.93  QFRR106 pKa = 11.84  LSGQVTEE113 pKa = 4.48  VTPSSGATGEE123 pKa = 3.99  LSIPQRR129 pKa = 11.84  YY130 pKa = 9.06  RR131 pKa = 11.84  MLLVEE136 pKa = 5.49  KK137 pKa = 10.85  DD138 pKa = 3.32  MLEE141 pKa = 4.05  NEE143 pKa = 4.36  MFIAEE148 pKa = 4.23  HH149 pKa = 6.23  SLVV152 pKa = 3.44  
 16.55 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  4.817 
IPC2_protein 4.927 
IPC_protein 4.8 
Toseland    4.749 
ProMoST     4.927 
Dawson      4.774 
Bjellqvist  4.902 
Wikipedia   4.609 
Rodwell     4.698 
Grimsley    4.673 
Solomon     4.762 
Lehninger   4.724 
Nozaki      4.889 
DTASelect   4.978 
Thurlkill   4.736 
EMBOSS      4.66 
Sillero     4.965 
Patrickios  3.567 
IPC_peptide 4.774 
IPC2_peptide  4.978 
IPC2.peptide.svr19  4.849 
 Protein with the highest isoelectric point: 
>tr|A0A060DA07|A0A060DA07_9RHAB Matrix protein OS=Fox fecal rhabdovirus OX=1504569 PE=4 SV=1MM1 pKa = 7.67  SCLTQDD7 pKa = 2.98  LRR9 pKa = 11.84  KK10 pKa = 9.76  EE11 pKa = 3.46  IRR13 pKa = 11.84  EE14 pKa = 3.97  RR15 pKa = 11.84  VSNSISQNPSVTTLIIIEE33 pKa = 4.3  VILWVGTLAYY43 pKa = 9.68  ISIALGCHH51 pKa = 6.55  RR52 pKa = 11.84  YY53 pKa = 8.8  LQARR57 pKa = 11.84  IKK59 pKa = 10.85  NSVEE63 pKa = 3.08  RR64 pKa = 11.84  RR65 pKa = 11.84  QRR67 pKa = 11.84  FRR69 pKa = 11.84  GDD71 pKa = 3.22  GVLLPDD77 pKa = 4.42  TGPAA81 pKa = 3.32  
 9.14 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.237 
IPC2_protein 8.96 
IPC_protein 9.326 
Toseland    9.516 
ProMoST     9.428 
Dawson      9.809 
Bjellqvist  9.677 
Wikipedia   10.028 
Rodwell     9.897 
Grimsley    9.882 
Solomon     9.97 
Lehninger   9.94 
Nozaki      9.794 
DTASelect   9.604 
Thurlkill   9.677 
EMBOSS      9.955 
Sillero     9.823 
Patrickios  7.614 
IPC_peptide 9.955 
IPC2_peptide  8.99 
IPC2.peptide.svr19  7.736 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        8 
0
8 
4815
76
2196
601.9
67.03
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        7.207 ± 0.664
1.786 ± 0.267
4.777 ± 0.382
6.459 ± 0.419
3.718 ± 0.436
6.604 ± 0.266
2.388 ± 0.214
6.044 ± 0.64
5.483 ± 0.625
9.844 ± 0.429
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        2.575 ± 0.217
3.427 ± 0.268
5.732 ± 0.554
2.866 ± 0.133
5.379 ± 0.275
9.076 ± 0.536
5.421 ± 0.23
6.293 ± 0.628
1.848 ± 0.187
3.074 ± 0.328
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here