Fox fecal rhabdovirus
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A060D558|A0A060D558_9RHAB Alpha 1 protein OS=Fox fecal rhabdovirus OX=1504569 PE=4 SV=1
MM1 pKa = 7.47 SPQHH5 pKa = 6.15 GASALISGVDD15 pKa = 3.49 PDD17 pKa = 4.47 HH18 pKa = 6.95 FSLIIEE24 pKa = 4.65 SYY26 pKa = 9.84 PILVGNYY33 pKa = 8.63 AATLILTVTLVLSFSLATIIFFFSSVNNLTDD64 pKa = 2.92 IRR66 pKa = 11.84 GVLIYY71 pKa = 10.6 HH72 pKa = 7.24 LGNEE76 pKa = 4.04 VSEE79 pKa = 4.67 FASHH83 pKa = 7.11 ALAAVCSAQSTSARR97 pKa = 11.84 LTRR100 pKa = 11.84 EE101 pKa = 3.85 LEE103 pKa = 3.93 QFRR106 pKa = 11.84 LSGQVTEE113 pKa = 4.48 VTPSSGATGEE123 pKa = 3.99 LSIPQRR129 pKa = 11.84 YY130 pKa = 9.06 RR131 pKa = 11.84 MLLVEE136 pKa = 5.49 KK137 pKa = 10.85 DD138 pKa = 3.32 MLEE141 pKa = 4.05 NEE143 pKa = 4.36 MFIAEE148 pKa = 4.23 HH149 pKa = 6.23 SLVV152 pKa = 3.44
Molecular weight: 16.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.817
IPC2_protein 4.927
IPC_protein 4.8
Toseland 4.749
ProMoST 4.927
Dawson 4.774
Bjellqvist 4.902
Wikipedia 4.609
Rodwell 4.698
Grimsley 4.673
Solomon 4.762
Lehninger 4.724
Nozaki 4.889
DTASelect 4.978
Thurlkill 4.736
EMBOSS 4.66
Sillero 4.965
Patrickios 3.567
IPC_peptide 4.774
IPC2_peptide 4.978
IPC2.peptide.svr19 4.849
Protein with the highest isoelectric point:
>tr|A0A060DA07|A0A060DA07_9RHAB Matrix protein OS=Fox fecal rhabdovirus OX=1504569 PE=4 SV=1
MM1 pKa = 7.67 SCLTQDD7 pKa = 2.98 LRR9 pKa = 11.84 KK10 pKa = 9.76 EE11 pKa = 3.46 IRR13 pKa = 11.84 EE14 pKa = 3.97 RR15 pKa = 11.84 VSNSISQNPSVTTLIIIEE33 pKa = 4.3 VILWVGTLAYY43 pKa = 9.68 ISIALGCHH51 pKa = 6.55 RR52 pKa = 11.84 YY53 pKa = 8.8 LQARR57 pKa = 11.84 IKK59 pKa = 10.85 NSVEE63 pKa = 3.08 RR64 pKa = 11.84 RR65 pKa = 11.84 QRR67 pKa = 11.84 FRR69 pKa = 11.84 GDD71 pKa = 3.22 GVLLPDD77 pKa = 4.42 TGPAA81 pKa = 3.32
Molecular weight: 9.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.237
IPC2_protein 8.96
IPC_protein 9.326
Toseland 9.516
ProMoST 9.428
Dawson 9.809
Bjellqvist 9.677
Wikipedia 10.028
Rodwell 9.897
Grimsley 9.882
Solomon 9.97
Lehninger 9.94
Nozaki 9.794
DTASelect 9.604
Thurlkill 9.677
EMBOSS 9.955
Sillero 9.823
Patrickios 7.614
IPC_peptide 9.955
IPC2_peptide 8.99
IPC2.peptide.svr19 7.736
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
4815
76
2196
601.9
67.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.207 ± 0.664
1.786 ± 0.267
4.777 ± 0.382
6.459 ± 0.419
3.718 ± 0.436
6.604 ± 0.266
2.388 ± 0.214
6.044 ± 0.64
5.483 ± 0.625
9.844 ± 0.429
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.575 ± 0.217
3.427 ± 0.268
5.732 ± 0.554
2.866 ± 0.133
5.379 ± 0.275
9.076 ± 0.536
5.421 ± 0.23
6.293 ± 0.628
1.848 ± 0.187
3.074 ± 0.328
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here