Hortaea werneckii

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Dothideomycetidae; Mycosphaerellales;

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16466 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3M6YG17|A0A3M6YG17_HORWE Uncharacterized protein (Fragment) OS=Hortaea werneckii OX=91943 GN=D0866_15707 PE=4 SV=1
MM1 pKa = 7.76APITNFLASSLALLSSTALAANPIKK26 pKa = 10.73VQEE29 pKa = 4.13QEE31 pKa = 4.6FIDD34 pKa = 3.92TKK36 pKa = 9.1TDD38 pKa = 2.94EE39 pKa = 4.34RR40 pKa = 11.84FVIVGVDD47 pKa = 3.48YY48 pKa = 11.34QPGGSAAVGTGNGDD62 pKa = 3.77PLSNADD68 pKa = 3.26EE69 pKa = 4.41CRR71 pKa = 11.84RR72 pKa = 11.84DD73 pKa = 3.39AALMQNMGVNTIRR86 pKa = 11.84SYY88 pKa = 11.79NLNPDD93 pKa = 3.97LNHH96 pKa = 7.18DD97 pKa = 3.77EE98 pKa = 4.55CASIFNSVGIYY109 pKa = 9.31MIIDD113 pKa = 3.43VNSPLEE119 pKa = 3.99GGYY122 pKa = 10.05INRR125 pKa = 11.84DD126 pKa = 3.81DD127 pKa = 4.67PSSTYY132 pKa = 10.32NSEE135 pKa = 3.67YY136 pKa = 10.17LKK138 pKa = 10.76RR139 pKa = 11.84VFTVIEE145 pKa = 4.36GFKK148 pKa = 10.53SYY150 pKa = 11.03PNTLGFFAGNEE161 pKa = 4.25IINDD165 pKa = 3.91LATASEE171 pKa = 4.2NPQYY175 pKa = 10.74IRR177 pKa = 11.84AVQRR181 pKa = 11.84DD182 pKa = 3.95MKK184 pKa = 10.97QYY186 pKa = 10.02IAQNADD192 pKa = 3.23RR193 pKa = 11.84EE194 pKa = 4.51IPVGYY199 pKa = 9.75SAADD203 pKa = 3.37VRR205 pKa = 11.84DD206 pKa = 3.71VLEE209 pKa = 4.5DD210 pKa = 3.0TWAYY214 pKa = 8.7LQCDD218 pKa = 3.43NSNNGDD224 pKa = 3.42SDD226 pKa = 3.75SRR228 pKa = 11.84SDD230 pKa = 4.05FFGLNSYY237 pKa = 8.04SWCGGDD243 pKa = 4.1ATFQSSGYY251 pKa = 9.85DD252 pKa = 3.27DD253 pKa = 4.27LVSMFSNTTIPVFFSEE269 pKa = 4.57YY270 pKa = 10.4GCIEE274 pKa = 3.68VTPRR278 pKa = 11.84VFDD281 pKa = 3.77EE282 pKa = 4.2VQALYY287 pKa = 10.02GPKK290 pKa = 8.14MTVLSGGLVYY300 pKa = 10.65EE301 pKa = 4.81WTQEE305 pKa = 3.83DD306 pKa = 3.54NGYY309 pKa = 10.25GLVNTTSDD317 pKa = 3.44GDD319 pKa = 3.83AQLTTDD325 pKa = 4.85FEE327 pKa = 4.72NLSSQYY333 pKa = 11.64AKK335 pKa = 11.12LNTTLLQSQNDD346 pKa = 3.85TATSLEE352 pKa = 4.2APQCDD357 pKa = 3.21ASLISSDD364 pKa = 3.8SFSTTFDD371 pKa = 3.33IPSQPDD377 pKa = 3.44GVADD381 pKa = 5.22LISNGVDD388 pKa = 3.16NAPSGSIVSVTQTSVDD404 pKa = 3.18ATVYY408 pKa = 9.46ATGGGKK414 pKa = 9.4ISGLQINPVEE424 pKa = 4.52GANAPGSGGNGGGSGLSTGAAPSGTSTSSNSGNGGSSSSQTSSGSEE470 pKa = 3.74ASNTSGGSNASGGSGGSSTSTGSGSSDD497 pKa = 3.0TTATSSGSAASSTEE511 pKa = 3.88SEE513 pKa = 4.27GTGFRR518 pKa = 11.84VDD520 pKa = 3.37GKK522 pKa = 8.88MASSAGLAAAIGAAAFALL540 pKa = 4.16

Molecular weight: 55.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3M6WIJ7|A0A3M6WIJ7_HORWE Metallophos domain-containing protein OS=Hortaea werneckii OX=91943 GN=D0863_12995 PE=4 SV=1
MM1 pKa = 7.88PSHH4 pKa = 6.89KK5 pKa = 10.44SFRR8 pKa = 11.84TKK10 pKa = 10.65VKK12 pKa = 9.82LAKK15 pKa = 9.79AQKK18 pKa = 8.73QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.15WRR45 pKa = 11.84KK46 pKa = 7.38TRR48 pKa = 11.84IGII51 pKa = 4.0

Molecular weight: 6.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16466

0

16466

8038627

50

4367

488.2

53.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.126 ± 0.014

1.135 ± 0.007

5.729 ± 0.014

6.432 ± 0.019

3.52 ± 0.013

7.48 ± 0.017

2.384 ± 0.007

4.275 ± 0.014

4.809 ± 0.019

8.42 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.271 ± 0.007

3.63 ± 0.009

6.16 ± 0.02

4.464 ± 0.015

6.065 ± 0.02

8.159 ± 0.027

5.931 ± 0.015

5.845 ± 0.014

1.428 ± 0.008

2.737 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski