Salinadaptatus halalkaliphilus
Average proteome isoelectric point is 4.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4165 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4S3TK88|A0A4S3TK88_9EURY Site-specific integrase OS=Salinadaptatus halalkaliphilus OX=2419781 GN=D8Y22_12840 PE=4 SV=1
MM1 pKa = 7.81 TDD3 pKa = 3.3 TPRR6 pKa = 11.84 LRR8 pKa = 11.84 SRR10 pKa = 11.84 RR11 pKa = 11.84 TLLASTGSLAALGIAGCLGDD31 pKa = 4.45 EE32 pKa = 4.39 NGGDD36 pKa = 4.53 SEE38 pKa = 6.32 DD39 pKa = 4.62 GDD41 pKa = 5.26 HH42 pKa = 7.98 DD43 pKa = 5.17 DD44 pKa = 5.93 HH45 pKa = 9.39 DD46 pKa = 6.79 DD47 pKa = 5.46 DD48 pKa = 7.48 DD49 pKa = 6.37 DD50 pKa = 5.47 DD51 pKa = 6.86 HH52 pKa = 8.99 DD53 pKa = 5.77 DD54 pKa = 3.81 DD55 pKa = 6.41 HH56 pKa = 9.11 DD57 pKa = 6.26 GDD59 pKa = 3.84 VHH61 pKa = 8.4 DD62 pKa = 5.84 EE63 pKa = 3.93 IGISEE68 pKa = 4.12 FEE70 pKa = 4.01 ILDD73 pKa = 3.8 RR74 pKa = 11.84 DD75 pKa = 3.55 LDD77 pKa = 4.02 EE78 pKa = 5.58 AVIAYY83 pKa = 7.86 VHH85 pKa = 6.69 GDD87 pKa = 3.2 HH88 pKa = 7.29 WDD90 pKa = 3.6 HH91 pKa = 6.95 GPLEE95 pKa = 4.57 VPHH98 pKa = 6.93 GDD100 pKa = 3.68 YY101 pKa = 11.19 VSVGAYY107 pKa = 10.22 VEE109 pKa = 5.65 DD110 pKa = 5.19 DD111 pKa = 4.68 DD112 pKa = 6.07 GDD114 pKa = 4.08 EE115 pKa = 4.24 VALGDD120 pKa = 4.93 DD121 pKa = 3.76 IEE123 pKa = 4.62 LAIEE127 pKa = 4.25 PADD130 pKa = 3.83 DD131 pKa = 3.75 AVSVDD136 pKa = 3.13 GHH138 pKa = 6.51 GDD140 pKa = 3.24 HH141 pKa = 5.93 VHH143 pKa = 6.31 VYY145 pKa = 10.46 GEE147 pKa = 4.04 EE148 pKa = 3.88 AGFTEE153 pKa = 5.16 VVVQLVDD160 pKa = 3.39 SGDD163 pKa = 3.52 VVYY166 pKa = 10.3 EE167 pKa = 4.13 SPEE170 pKa = 4.12 LEE172 pKa = 4.45 VAVDD176 pKa = 5.3 DD177 pKa = 5.11 YY178 pKa = 12.21 DD179 pKa = 6.13 HH180 pKa = 7.85 DD181 pKa = 4.14 DD182 pKa = 3.89 HH183 pKa = 8.01 GHH185 pKa = 6.06 GEE187 pKa = 4.02 VDD189 pKa = 3.27 EE190 pKa = 4.95 LKK192 pKa = 10.61 IIDD195 pKa = 3.98 RR196 pKa = 11.84 SEE198 pKa = 4.29 DD199 pKa = 3.2 PHH201 pKa = 8.4 EE202 pKa = 4.55 EE203 pKa = 3.67 VAEE206 pKa = 4.02 YY207 pKa = 10.7 HH208 pKa = 6.76 DD209 pKa = 4.1 GHH211 pKa = 6.94 WDD213 pKa = 3.39 GEE215 pKa = 4.41 LPHH218 pKa = 6.88 VHH220 pKa = 6.32 VDD222 pKa = 3.72 DD223 pKa = 5.52 NISLGAEE230 pKa = 4.01 FLDD233 pKa = 4.12 DD234 pKa = 5.06 HH235 pKa = 7.88 GDD237 pKa = 3.83 EE238 pKa = 4.55 IPIGGNEE245 pKa = 4.07 EE246 pKa = 4.07 YY247 pKa = 10.71 EE248 pKa = 4.31 LAVTLADD255 pKa = 4.01 DD256 pKa = 4.33 AEE258 pKa = 5.39 DD259 pKa = 4.97 GIVAIDD265 pKa = 4.72 PDD267 pKa = 4.28 EE268 pKa = 4.63 HH269 pKa = 5.39 WHH271 pKa = 6.48 GDD273 pKa = 3.16 HH274 pKa = 5.43 VHH276 pKa = 6.98 IYY278 pKa = 10.95 GEE280 pKa = 4.6 DD281 pKa = 3.57 EE282 pKa = 4.45 SEE284 pKa = 4.28 TEE286 pKa = 4.32 VVFKK290 pKa = 10.7 LWHH293 pKa = 7.23 DD294 pKa = 4.15 DD295 pKa = 3.56 HH296 pKa = 8.44 ADD298 pKa = 3.47 WEE300 pKa = 4.59 SAPIAVEE307 pKa = 4.29 VEE309 pKa = 4.15 DD310 pKa = 4.27 HH311 pKa = 6.61
Molecular weight: 34.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.686
IPC2_protein 3.745
IPC_protein 3.783
Toseland 3.567
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.706
Rodwell 3.617
Grimsley 3.465
Solomon 3.783
Lehninger 3.732
Nozaki 3.884
DTASelect 4.139
Thurlkill 3.617
EMBOSS 3.719
Sillero 3.91
Patrickios 1.481
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.799
Protein with the highest isoelectric point:
>tr|A0A4S3TH69|A0A4S3TH69_9EURY Uncharacterized protein OS=Salinadaptatus halalkaliphilus OX=2419781 GN=D8Y22_18565 PE=4 SV=1
MM1 pKa = 7.35 LPKK4 pKa = 9.82 RR5 pKa = 11.84 TVRR8 pKa = 11.84 CAVTRR13 pKa = 11.84 LKK15 pKa = 10.83 EE16 pKa = 3.94 SGLIEE21 pKa = 4.06 GRR23 pKa = 11.84 VSFRR27 pKa = 11.84 DD28 pKa = 3.45 ARR30 pKa = 11.84 QSVYY34 pKa = 11.32 SLTEE38 pKa = 3.87 DD39 pKa = 3.59 GKK41 pKa = 10.53 RR42 pKa = 11.84 VRR44 pKa = 11.84 TLPVEE49 pKa = 4.18 KK50 pKa = 10.66
Molecular weight: 5.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.331
IPC2_protein 9.531
IPC_protein 10.189
Toseland 10.745
ProMoST 10.511
Dawson 10.804
Bjellqvist 10.496
Wikipedia 10.994
Rodwell 11.038
Grimsley 10.847
Solomon 10.95
Lehninger 10.921
Nozaki 10.73
DTASelect 10.482
Thurlkill 10.73
EMBOSS 11.14
Sillero 10.745
Patrickios 10.847
IPC_peptide 10.95
IPC2_peptide 9.443
IPC2.peptide.svr19 8.719
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4165
0
4165
1243612
42
2865
298.6
32.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.439 ± 0.046
0.759 ± 0.011
8.733 ± 0.057
8.844 ± 0.058
3.2 ± 0.028
8.146 ± 0.038
2.04 ± 0.017
4.653 ± 0.031
1.7 ± 0.021
8.952 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.701 ± 0.019
2.292 ± 0.021
4.62 ± 0.026
2.673 ± 0.024
6.293 ± 0.038
5.603 ± 0.029
6.795 ± 0.033
8.72 ± 0.04
1.112 ± 0.013
2.724 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here