Bacillus phage vB_BceM_Bc431v3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Caeruleovirus; Bacillus virus Bc431

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 238 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4HN64|M4HN64_9CAUD Uncharacterized protein OS=Bacillus phage vB_BceM_Bc431v3 OX=1195072 GN=orf063 PE=4 SV=1
MM1 pKa = 7.65TEE3 pKa = 4.26LNCNEE8 pKa = 4.72VVSQWGNFGDD18 pKa = 4.13NNPIEE23 pKa = 4.48YY24 pKa = 8.13GTKK27 pKa = 9.59FVKK30 pKa = 10.57ADD32 pKa = 3.68TEE34 pKa = 4.4NEE36 pKa = 3.65GCYY39 pKa = 10.66YY40 pKa = 10.01FIQVINLEE48 pKa = 4.15WTIGEE53 pKa = 4.6DD54 pKa = 2.99GWFLVKK60 pKa = 10.6GYY62 pKa = 11.26VDD64 pKa = 6.23LNDD67 pKa = 5.26DD68 pKa = 3.96WIDD71 pKa = 2.95WKK73 pKa = 10.93AIKK76 pKa = 10.22EE77 pKa = 3.91FAGFDD82 pKa = 3.48NTCQDD87 pKa = 3.33TEE89 pKa = 4.18KK90 pKa = 10.61EE91 pKa = 4.43YY92 pKa = 10.42MVSALVEE99 pKa = 3.96YY100 pKa = 10.55YY101 pKa = 10.46GYY103 pKa = 10.71HH104 pKa = 5.98EE105 pKa = 5.23FGGEE109 pKa = 3.82PLSNEE114 pKa = 3.86FGVDD118 pKa = 3.54FNGVVNEE125 pKa = 4.14VKK127 pKa = 10.61KK128 pKa = 9.51EE129 pKa = 4.04AEE131 pKa = 4.39VITLLNEE138 pKa = 3.74YY139 pKa = 10.58GILLEE144 pKa = 4.58RR145 pKa = 11.84KK146 pKa = 9.49EE147 pKa = 4.41EE148 pKa = 4.05VLKK151 pKa = 8.77TTVIYY156 pKa = 10.75HH157 pKa = 5.93NEE159 pKa = 3.82SEE161 pKa = 4.47FFYY164 pKa = 10.42TLKK167 pKa = 10.93DD168 pKa = 3.62DD169 pKa = 3.94VEE171 pKa = 5.21GISEE175 pKa = 4.39TVDD178 pKa = 3.4GEE180 pKa = 4.66GVIHH184 pKa = 7.09LGQYY188 pKa = 8.32KK189 pKa = 9.67HH190 pKa = 6.51DD191 pKa = 4.88RR192 pKa = 11.84IVVSEE197 pKa = 4.33SLEE200 pKa = 3.91QLQTMLEE207 pKa = 4.48GYY209 pKa = 9.67DD210 pKa = 3.52VEE212 pKa = 5.06VMTEE216 pKa = 3.95EE217 pKa = 4.59EE218 pKa = 4.16EE219 pKa = 4.82EE220 pKa = 4.24EE221 pKa = 4.3DD222 pKa = 4.62NEE224 pKa = 4.34

Molecular weight: 25.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4HN77|M4HN77_9CAUD Uncharacterized protein OS=Bacillus phage vB_BceM_Bc431v3 OX=1195072 GN=orf129 PE=4 SV=1
MM1 pKa = 7.07TRR3 pKa = 11.84KK4 pKa = 8.35KK5 pKa = 8.89TGKK8 pKa = 8.68PNAVQRR14 pKa = 11.84SMSRR18 pKa = 11.84GLGARR23 pKa = 11.84GDD25 pKa = 3.72GSKK28 pKa = 10.93NINDD32 pKa = 3.96NGKK35 pKa = 9.56DD36 pKa = 3.0AYY38 pKa = 9.84KK39 pKa = 10.5RR40 pKa = 11.84SRR42 pKa = 11.84QSTKK46 pKa = 9.97GNYY49 pKa = 8.51RR50 pKa = 11.84VAFTEE55 pKa = 4.09LFEE58 pKa = 4.73KK59 pKa = 9.68VTEE62 pKa = 4.2RR63 pKa = 11.84DD64 pKa = 3.06LSLKK68 pKa = 8.53HH69 pKa = 6.14TYY71 pKa = 10.77AKK73 pKa = 10.7DD74 pKa = 3.33LLSNHH79 pKa = 6.46LKK81 pKa = 10.72VPVDD85 pKa = 3.96MITLKK90 pKa = 10.81VKK92 pKa = 10.65RR93 pKa = 11.84KK94 pKa = 7.91PQQTLTSVDD103 pKa = 3.07EE104 pKa = 4.17VFYY107 pKa = 11.32VKK109 pKa = 10.46VGSDD113 pKa = 2.85IYY115 pKa = 10.64GKK117 pKa = 10.74VSIRR121 pKa = 11.84VQRR124 pKa = 11.84VLRR127 pKa = 11.84GNMLIFIFQEE137 pKa = 4.16KK138 pKa = 9.74KK139 pKa = 10.93SSIKK143 pKa = 10.5PPTKK147 pKa = 10.07SYY149 pKa = 10.81SRR151 pKa = 11.84KK152 pKa = 8.16TGFKK156 pKa = 10.19KK157 pKa = 9.34KK158 pKa = 8.83TSSQISQTKK167 pKa = 8.54RR168 pKa = 11.84KK169 pKa = 9.51SYY171 pKa = 10.48HH172 pKa = 5.43SRR174 pKa = 11.84QGGTNN179 pKa = 3.08

Molecular weight: 20.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

238

0

238

47699

27

1355

200.4

22.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.128 ± 0.177

0.801 ± 0.061

6.218 ± 0.114

7.853 ± 0.286

3.885 ± 0.111

6.658 ± 0.266

1.742 ± 0.088

6.359 ± 0.126

7.906 ± 0.21

8.032 ± 0.197

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.818 ± 0.089

5.363 ± 0.181

3.109 ± 0.163

3.725 ± 0.108

4.371 ± 0.141

5.755 ± 0.15

6.401 ± 0.224

7.26 ± 0.164

1.218 ± 0.07

4.398 ± 0.134
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski