Amino acid dipepetide frequency for Bacillus phage vB_BceM_Bc431v3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.977AlaAla: 2.977 ± 0.397
0.398AlaCys: 0.398 ± 0.092
3.417AlaAsp: 3.417 ± 0.266
4.508AlaGlu: 4.508 ± 0.308
2.6AlaPhe: 2.6 ± 0.171
4.675AlaGly: 4.675 ± 0.464
0.943AlaHis: 0.943 ± 0.114
3.732AlaIle: 3.732 ± 0.279
5.22AlaLys: 5.22 ± 0.369
5.178AlaLeu: 5.178 ± 0.329
1.489AlaMet: 1.489 ± 0.188
3.292AlaAsn: 3.292 ± 0.31
2.285AlaPro: 2.285 ± 0.362
2.767AlaGln: 2.767 ± 0.291
2.411AlaArg: 2.411 ± 0.242
3.229AlaSer: 3.229 ± 0.388
4.717AlaThr: 4.717 ± 0.38
3.92AlaVal: 3.92 ± 0.321
0.776AlaTrp: 0.776 ± 0.098
2.746AlaTyr: 2.746 ± 0.255
0.0AlaXaa: 0.0 ± 0.0
Cys
0.294CysAla: 0.294 ± 0.077
0.084CysCys: 0.084 ± 0.038
0.629CysAsp: 0.629 ± 0.137
0.545CysGlu: 0.545 ± 0.101
0.252CysPhe: 0.252 ± 0.08
0.692CysGly: 0.692 ± 0.159
0.231CysHis: 0.231 ± 0.068
0.566CysIle: 0.566 ± 0.12
0.524CysLys: 0.524 ± 0.128
0.503CysLeu: 0.503 ± 0.103
0.168CysMet: 0.168 ± 0.066
0.356CysAsn: 0.356 ± 0.083
0.294CysPro: 0.294 ± 0.067
0.189CysGln: 0.189 ± 0.065
0.356CysArg: 0.356 ± 0.08
0.566CysSer: 0.566 ± 0.113
0.566CysThr: 0.566 ± 0.114
0.587CysVal: 0.587 ± 0.104
0.126CysTrp: 0.126 ± 0.052
0.482CysTyr: 0.482 ± 0.096
0.0CysXaa: 0.0 ± 0.0
Asp
3.69AspAla: 3.69 ± 0.285
0.503AspCys: 0.503 ± 0.11
3.522AspAsp: 3.522 ± 0.268
4.57AspGlu: 4.57 ± 0.289
2.579AspPhe: 2.579 ± 0.24
4.319AspGly: 4.319 ± 0.355
0.608AspHis: 0.608 ± 0.1
5.157AspIle: 5.157 ± 0.352
5.64AspLys: 5.64 ± 0.414
4.864AspLeu: 4.864 ± 0.302
1.593AspMet: 1.593 ± 0.175
3.711AspAsn: 3.711 ± 0.291
2.076AspPro: 2.076 ± 0.234
1.342AspGln: 1.342 ± 0.137
2.558AspArg: 2.558 ± 0.261
3.396AspSer: 3.396 ± 0.271
3.354AspThr: 3.354 ± 0.314
4.298AspVal: 4.298 ± 0.332
0.943AspTrp: 0.943 ± 0.142
3.459AspTyr: 3.459 ± 0.227
0.0AspXaa: 0.0 ± 0.0
Glu
3.983GluAla: 3.983 ± 0.322
0.692GluCys: 0.692 ± 0.138
5.346GluAsp: 5.346 ± 0.351
8.743GluGlu: 8.743 ± 0.717
2.788GluPhe: 2.788 ± 0.25
5.116GluGly: 5.116 ± 0.372
1.363GluHis: 1.363 ± 0.168
4.906GluIle: 4.906 ± 0.332
6.248GluLys: 6.248 ± 0.407
7.38GluLeu: 7.38 ± 0.407
2.684GluMet: 2.684 ± 0.235
3.627GluAsn: 3.627 ± 0.312
2.034GluPro: 2.034 ± 0.284
3.25GluGln: 3.25 ± 0.255
3.438GluArg: 3.438 ± 0.273
3.459GluSer: 3.459 ± 0.303
3.292GluThr: 3.292 ± 0.241
5.87GluVal: 5.87 ± 0.461
0.922GluTrp: 0.922 ± 0.136
3.396GluTyr: 3.396 ± 0.319
0.0GluXaa: 0.0 ± 0.0
Phe
2.138PheAla: 2.138 ± 0.189
0.44PheCys: 0.44 ± 0.095
2.83PheAsp: 2.83 ± 0.228
2.705PheGlu: 2.705 ± 0.199
1.384PhePhe: 1.384 ± 0.204
2.285PheGly: 2.285 ± 0.211
0.86PheHis: 0.86 ± 0.154
2.516PheIle: 2.516 ± 0.251
3.04PheLys: 3.04 ± 0.271
2.725PheLeu: 2.725 ± 0.249
1.069PheMet: 1.069 ± 0.146
2.264PheAsn: 2.264 ± 0.26
1.132PhePro: 1.132 ± 0.142
1.279PheGln: 1.279 ± 0.169
1.572PheArg: 1.572 ± 0.175
2.914PheSer: 2.914 ± 0.291
2.809PheThr: 2.809 ± 0.25
2.746PheVal: 2.746 ± 0.255
0.419PheTrp: 0.419 ± 0.091
1.719PheTyr: 1.719 ± 0.181
0.0PheXaa: 0.0 ± 0.0
Gly
4.298GlyAla: 4.298 ± 0.492
0.608GlyCys: 0.608 ± 0.114
3.501GlyAsp: 3.501 ± 0.279
4.151GlyGlu: 4.151 ± 0.259
2.809GlyPhe: 2.809 ± 0.259
5.388GlyGly: 5.388 ± 0.699
1.216GlyHis: 1.216 ± 0.156
4.109GlyIle: 4.109 ± 0.343
5.346GlyLys: 5.346 ± 0.356
4.696GlyLeu: 4.696 ± 0.343
2.243GlyMet: 2.243 ± 0.283
4.088GlyAsn: 4.088 ± 0.354
0.105GlyPro: 0.105 ± 0.045
2.558GlyGln: 2.558 ± 0.282
2.705GlyArg: 2.705 ± 0.234
4.969GlySer: 4.969 ± 0.29
5.053GlyThr: 5.053 ± 0.369
4.57GlyVal: 4.57 ± 0.316
1.069GlyTrp: 1.069 ± 0.139
3.103GlyTyr: 3.103 ± 0.29
0.0GlyXaa: 0.0 ± 0.0
His
0.881HisAla: 0.881 ± 0.138
0.105HisCys: 0.105 ± 0.046
0.86HisAsp: 0.86 ± 0.132
1.342HisGlu: 1.342 ± 0.17
0.65HisPhe: 0.65 ± 0.129
0.943HisGly: 0.943 ± 0.143
0.314HisHis: 0.314 ± 0.093
1.279HisIle: 1.279 ± 0.185
1.216HisLys: 1.216 ± 0.168
1.551HisLeu: 1.551 ± 0.203
0.461HisMet: 0.461 ± 0.105
1.069HisAsn: 1.069 ± 0.139
0.734HisPro: 0.734 ± 0.131
0.44HisGln: 0.44 ± 0.098
1.09HisArg: 1.09 ± 0.142
0.734HisSer: 0.734 ± 0.125
1.111HisThr: 1.111 ± 0.167
1.468HisVal: 1.468 ± 0.199
0.273HisTrp: 0.273 ± 0.077
0.902HisTyr: 0.902 ± 0.143
0.0HisXaa: 0.0 ± 0.0
Ile
4.424IleAla: 4.424 ± 0.358
0.398IleCys: 0.398 ± 0.085
4.172IleAsp: 4.172 ± 0.307
5.199IleGlu: 5.199 ± 0.324
1.992IlePhe: 1.992 ± 0.206
3.816IleGly: 3.816 ± 0.283
1.216IleHis: 1.216 ± 0.154
3.564IleIle: 3.564 ± 0.316
5.053IleLys: 5.053 ± 0.317
4.214IleLeu: 4.214 ± 0.349
1.887IleMet: 1.887 ± 0.171
3.858IleAsn: 3.858 ± 0.352
2.6IlePro: 2.6 ± 0.239
2.222IleGln: 2.222 ± 0.17
2.998IleArg: 2.998 ± 0.261
3.962IleSer: 3.962 ± 0.327
4.508IleThr: 4.508 ± 0.327
4.822IleVal: 4.822 ± 0.324
0.524IleTrp: 0.524 ± 0.111
2.159IleTyr: 2.159 ± 0.209
0.0IleXaa: 0.0 ± 0.0
Lys
5.011LysAla: 5.011 ± 0.326
0.335LysCys: 0.335 ± 0.085
5.64LysAsp: 5.64 ± 0.378
8.281LysGlu: 8.281 ± 0.603
2.579LysPhe: 2.579 ± 0.27
4.78LysGly: 4.78 ± 0.402
1.363LysHis: 1.363 ± 0.189
4.214LysIle: 4.214 ± 0.286
6.415LysLys: 6.415 ± 0.445
6.185LysLeu: 6.185 ± 0.401
2.684LysMet: 2.684 ± 0.213
3.459LysAsn: 3.459 ± 0.296
2.516LysPro: 2.516 ± 0.248
3.627LysGln: 3.627 ± 0.263
3.816LysArg: 3.816 ± 0.331
3.9LysSer: 3.9 ± 0.398
4.235LysThr: 4.235 ± 0.309
5.807LysVal: 5.807 ± 0.367
0.964LysTrp: 0.964 ± 0.14
3.25LysTyr: 3.25 ± 0.296
0.0LysXaa: 0.0 ± 0.0
Leu
5.577LeuAla: 5.577 ± 0.371
0.65LeuCys: 0.65 ± 0.125
5.074LeuAsp: 5.074 ± 0.325
6.835LeuGlu: 6.835 ± 0.368
3.292LeuPhe: 3.292 ± 0.246
4.969LeuGly: 4.969 ± 0.307
1.635LeuHis: 1.635 ± 0.186
4.612LeuIle: 4.612 ± 0.458
5.954LeuLys: 5.954 ± 0.341
6.709LeuLeu: 6.709 ± 0.512
2.138LeuMet: 2.138 ± 0.245
3.669LeuAsn: 3.669 ± 0.276
2.83LeuPro: 2.83 ± 0.241
3.69LeuGln: 3.69 ± 0.267
3.501LeuArg: 3.501 ± 0.293
4.424LeuSer: 4.424 ± 0.284
5.304LeuThr: 5.304 ± 0.328
5.493LeuVal: 5.493 ± 0.372
0.818LeuTrp: 0.818 ± 0.14
3.124LeuTyr: 3.124 ± 0.253
0.0LeuXaa: 0.0 ± 0.0
Met
1.866MetAla: 1.866 ± 0.175
0.168MetCys: 0.168 ± 0.066
1.782MetAsp: 1.782 ± 0.203
1.971MetGlu: 1.971 ± 0.188
1.3MetPhe: 1.3 ± 0.165
1.572MetGly: 1.572 ± 0.189
0.44MetHis: 0.44 ± 0.093
2.034MetIle: 2.034 ± 0.228
2.516MetLys: 2.516 ± 0.217
2.306MetLeu: 2.306 ± 0.202
0.671MetMet: 0.671 ± 0.121
1.929MetAsn: 1.929 ± 0.212
0.713MetPro: 0.713 ± 0.166
0.943MetGln: 0.943 ± 0.145
1.153MetArg: 1.153 ± 0.148
1.803MetSer: 1.803 ± 0.19
2.076MetThr: 2.076 ± 0.233
1.614MetVal: 1.614 ± 0.153
0.294MetTrp: 0.294 ± 0.078
1.027MetTyr: 1.027 ± 0.144
0.0MetXaa: 0.0 ± 0.0
Asn
3.271AsnAla: 3.271 ± 0.311
0.545AsnCys: 0.545 ± 0.162
2.956AsnAsp: 2.956 ± 0.289
3.166AsnGlu: 3.166 ± 0.248
2.097AsnPhe: 2.097 ± 0.228
4.654AsnGly: 4.654 ± 0.401
0.881AsnHis: 0.881 ± 0.123
3.543AsnIle: 3.543 ± 0.285
3.92AsnLys: 3.92 ± 0.293
4.277AsnLeu: 4.277 ± 0.275
1.677AsnMet: 1.677 ± 0.212
2.956AsnAsn: 2.956 ± 0.308
2.285AsnPro: 2.285 ± 0.24
1.614AsnGln: 1.614 ± 0.189
2.6AsnArg: 2.6 ± 0.262
2.977AsnSer: 2.977 ± 0.265
3.522AsnThr: 3.522 ± 0.261
3.837AsnVal: 3.837 ± 0.327
0.566AsnTrp: 0.566 ± 0.111
2.285AsnTyr: 2.285 ± 0.211
0.0AsnXaa: 0.0 ± 0.0
Pro
1.593ProAla: 1.593 ± 0.235
0.294ProCys: 0.294 ± 0.077
1.95ProAsp: 1.95 ± 0.214
2.18ProGlu: 2.18 ± 0.214
1.321ProPhe: 1.321 ± 0.184
1.3ProGly: 1.3 ± 0.163
0.524ProHis: 0.524 ± 0.103
1.992ProIle: 1.992 ± 0.19
2.684ProLys: 2.684 ± 0.216
2.369ProLeu: 2.369 ± 0.241
0.964ProMet: 0.964 ± 0.179
1.782ProAsn: 1.782 ± 0.189
0.713ProPro: 0.713 ± 0.117
1.384ProGln: 1.384 ± 0.208
1.195ProArg: 1.195 ± 0.139
1.908ProSer: 1.908 ± 0.232
2.621ProThr: 2.621 ± 0.333
2.621ProVal: 2.621 ± 0.315
0.252ProTrp: 0.252 ± 0.073
1.447ProTyr: 1.447 ± 0.178
0.0ProXaa: 0.0 ± 0.0
Gln
2.893GlnAla: 2.893 ± 0.263
0.252GlnCys: 0.252 ± 0.063
2.097GlnAsp: 2.097 ± 0.228
3.103GlnGlu: 3.103 ± 0.285
1.405GlnPhe: 1.405 ± 0.169
2.243GlnGly: 2.243 ± 0.206
0.881GlnHis: 0.881 ± 0.169
2.076GlnIle: 2.076 ± 0.19
2.201GlnLys: 2.201 ± 0.212
3.354GlnLeu: 3.354 ± 0.322
1.09GlnMet: 1.09 ± 0.135
1.782GlnAsn: 1.782 ± 0.183
1.468GlnPro: 1.468 ± 0.323
1.866GlnGln: 1.866 ± 0.233
1.698GlnArg: 1.698 ± 0.166
2.264GlnSer: 2.264 ± 0.233
1.929GlnThr: 1.929 ± 0.204
2.788GlnVal: 2.788 ± 0.228
0.503GlnTrp: 0.503 ± 0.09
1.363GlnTyr: 1.363 ± 0.164
0.0GlnXaa: 0.0 ± 0.0
Arg
2.474ArgAla: 2.474 ± 0.214
0.335ArgCys: 0.335 ± 0.095
2.222ArgAsp: 2.222 ± 0.215
3.962ArgGlu: 3.962 ± 0.338
1.908ArgPhe: 1.908 ± 0.191
2.956ArgGly: 2.956 ± 0.227
0.524ArgHis: 0.524 ± 0.102
2.663ArgIle: 2.663 ± 0.246
3.585ArgLys: 3.585 ± 0.307
3.292ArgLeu: 3.292 ± 0.266
1.53ArgMet: 1.53 ± 0.182
2.117ArgAsn: 2.117 ± 0.208
1.258ArgPro: 1.258 ± 0.175
1.698ArgGln: 1.698 ± 0.196
2.055ArgArg: 2.055 ± 0.199
2.201ArgSer: 2.201 ± 0.19
2.642ArgThr: 2.642 ± 0.234
3.501ArgVal: 3.501 ± 0.302
0.608ArgTrp: 0.608 ± 0.114
2.18ArgTyr: 2.18 ± 0.227
0.0ArgXaa: 0.0 ± 0.0
Ser
3.543SerAla: 3.543 ± 0.256
0.44SerCys: 0.44 ± 0.096
3.396SerAsp: 3.396 ± 0.265
3.543SerGlu: 3.543 ± 0.292
2.83SerPhe: 2.83 ± 0.226
4.172SerGly: 4.172 ± 0.351
0.839SerHis: 0.839 ± 0.123
3.669SerIle: 3.669 ± 0.326
4.067SerLys: 4.067 ± 0.241
5.157SerLeu: 5.157 ± 0.328
1.384SerMet: 1.384 ± 0.167
2.579SerAsn: 2.579 ± 0.29
1.866SerPro: 1.866 ± 0.182
1.719SerGln: 1.719 ± 0.179
2.705SerArg: 2.705 ± 0.24
4.424SerSer: 4.424 ± 0.642
3.606SerThr: 3.606 ± 0.31
4.067SerVal: 4.067 ± 0.286
0.902SerTrp: 0.902 ± 0.165
2.642SerTyr: 2.642 ± 0.26
0.0SerXaa: 0.0 ± 0.0
Thr
3.858ThrAla: 3.858 ± 0.36
0.524ThrCys: 0.524 ± 0.114
3.795ThrAsp: 3.795 ± 0.287
4.403ThrGlu: 4.403 ± 0.33
2.285ThrPhe: 2.285 ± 0.22
4.885ThrGly: 4.885 ± 0.342
1.069ThrHis: 1.069 ± 0.168
4.948ThrIle: 4.948 ± 0.315
4.487ThrLys: 4.487 ± 0.283
5.199ThrLeu: 5.199 ± 0.318
1.489ThrMet: 1.489 ± 0.149
3.837ThrAsn: 3.837 ± 0.364
2.663ThrPro: 2.663 ± 0.277
2.013ThrGln: 2.013 ± 0.213
2.725ThrArg: 2.725 ± 0.275
3.166ThrSer: 3.166 ± 0.306
3.711ThrThr: 3.711 ± 0.312
5.241ThrVal: 5.241 ± 0.318
0.671ThrTrp: 0.671 ± 0.11
3.04ThrTyr: 3.04 ± 0.327
0.0ThrXaa: 0.0 ± 0.0
Val
5.011ValAla: 5.011 ± 0.33
0.545ValCys: 0.545 ± 0.104
4.759ValAsp: 4.759 ± 0.312
5.556ValGlu: 5.556 ± 0.411
2.621ValPhe: 2.621 ± 0.22
4.361ValGly: 4.361 ± 0.348
1.342ValHis: 1.342 ± 0.155
4.382ValIle: 4.382 ± 0.294
5.954ValLys: 5.954 ± 0.363
5.724ValLeu: 5.724 ± 0.354
1.489ValMet: 1.489 ± 0.197
4.004ValAsn: 4.004 ± 0.335
2.474ValPro: 2.474 ± 0.263
2.705ValGln: 2.705 ± 0.224
2.914ValArg: 2.914 ± 0.253
3.983ValSer: 3.983 ± 0.345
5.367ValThr: 5.367 ± 0.416
5.346ValVal: 5.346 ± 0.407
0.902ValTrp: 0.902 ± 0.145
3.166ValTyr: 3.166 ± 0.267
0.0ValXaa: 0.0 ± 0.0
Trp
0.776TrpAla: 0.776 ± 0.123
0.189TrpCys: 0.189 ± 0.063
1.069TrpAsp: 1.069 ± 0.157
1.006TrpGlu: 1.006 ± 0.134
0.524TrpPhe: 0.524 ± 0.113
0.797TrpGly: 0.797 ± 0.137
0.231TrpHis: 0.231 ± 0.074
0.65TrpIle: 0.65 ± 0.12
1.069TrpLys: 1.069 ± 0.122
0.797TrpLeu: 0.797 ± 0.137
0.273TrpMet: 0.273 ± 0.073
0.671TrpAsn: 0.671 ± 0.136
0.0TrpPro: 0.0 ± 0.0
0.524TrpGln: 0.524 ± 0.105
0.545TrpArg: 0.545 ± 0.094
0.587TrpSer: 0.587 ± 0.116
0.713TrpThr: 0.713 ± 0.127
0.922TrpVal: 0.922 ± 0.162
0.252TrpTrp: 0.252 ± 0.068
0.587TrpTyr: 0.587 ± 0.107
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.725TyrAla: 2.725 ± 0.207
0.503TyrCys: 0.503 ± 0.104
3.166TyrAsp: 3.166 ± 0.247
2.767TyrGlu: 2.767 ± 0.222
1.635TyrPhe: 1.635 ± 0.204
2.663TyrGly: 2.663 ± 0.237
0.943TyrHis: 0.943 ± 0.137
2.977TyrIle: 2.977 ± 0.206
3.92TyrLys: 3.92 ± 0.347
3.837TyrLeu: 3.837 ± 0.279
1.174TyrMet: 1.174 ± 0.129
2.579TyrAsn: 2.579 ± 0.217
1.048TyrPro: 1.048 ± 0.144
1.426TyrGln: 1.426 ± 0.17
1.677TyrArg: 1.677 ± 0.217
2.684TyrSer: 2.684 ± 0.217
2.872TyrThr: 2.872 ± 0.226
3.082TyrVal: 3.082 ± 0.269
0.398TyrTrp: 0.398 ± 0.092
1.908TyrTyr: 1.908 ± 0.211
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 238 proteins (47699 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski