Stylonychia lemnae (Ciliate)

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata; Spirotrichea; Stichotrichia; Sporadotrichida; Oxytrichidae; Stylonychinae; Stylonychia

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20720 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A078AFN0|A0A078AFN0_STYLE Uncharacterized protein OS=Stylonychia lemnae OX=5949 GN=Contig16152.g17209 PE=4 SV=1
MM1 pKa = 6.94VKK3 pKa = 9.94IVGMEE8 pKa = 3.92DD9 pKa = 3.09MKK11 pKa = 11.29NLLMINSDD19 pKa = 2.9IFEE22 pKa = 4.27QPIEE26 pKa = 4.12DD27 pKa = 3.72QQIDD31 pKa = 3.71EE32 pKa = 5.12SYY34 pKa = 11.59DD35 pKa = 3.61LDD37 pKa = 3.57EE38 pKa = 4.36TSIFRR43 pKa = 11.84IQVDD47 pKa = 3.3CDD49 pKa = 3.14EE50 pKa = 4.92PQFQFEE56 pKa = 4.41IDD58 pKa = 3.35NDD60 pKa = 3.18IDD62 pKa = 4.15LNAFLVTINSSNQVINDD79 pKa = 4.03TYY81 pKa = 11.29NSLGGFSYY89 pKa = 11.43NDD91 pKa = 3.51LQLEE95 pKa = 4.32PNPDD99 pKa = 4.15RR100 pKa = 11.84IFMPIIYY107 pKa = 9.78GKK109 pKa = 10.12DD110 pKa = 3.24GQICEE115 pKa = 4.23DD116 pKa = 3.39QYY118 pKa = 11.69LIDD121 pKa = 5.83GLWEE125 pKa = 4.14NNKK128 pKa = 10.35SNGHH132 pKa = 5.3GQVVWNNGNYY142 pKa = 10.44YY143 pKa = 9.76IGEE146 pKa = 4.13WKK148 pKa = 10.19DD149 pKa = 3.75DD150 pKa = 3.6QSHH153 pKa = 7.5GIGQFYY159 pKa = 10.98NEE161 pKa = 4.86EE162 pKa = 3.85EE163 pKa = 3.99NKK165 pKa = 10.09FYY167 pKa = 10.76KK168 pKa = 10.73GEE170 pKa = 4.08QEE172 pKa = 3.54FDD174 pKa = 3.19QYY176 pKa = 12.03NGLGKK181 pKa = 9.79MIYY184 pKa = 9.98NDD186 pKa = 3.21GSIYY190 pKa = 9.81YY191 pKa = 8.1GQWEE195 pKa = 4.51SNQRR199 pKa = 11.84SRR201 pKa = 11.84FGVFIFPEE209 pKa = 4.67GDD211 pKa = 3.4SEE213 pKa = 4.34EE214 pKa = 4.26RR215 pKa = 3.49

Molecular weight: 25.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A078A6P9|A0A078A6P9_STYLE Bifunctional polynucleotide phosphatase kinase-like OS=Stylonychia lemnae OX=5949 GN=Contig18063.g19195 PE=4 SV=1
MM1 pKa = 7.34ARR3 pKa = 11.84SRR5 pKa = 11.84PAGAAKK11 pKa = 10.1KK12 pKa = 9.24VVKK15 pKa = 10.23RR16 pKa = 11.84VSKK19 pKa = 10.51KK20 pKa = 9.66AAASKK25 pKa = 10.68KK26 pKa = 8.57IGKK29 pKa = 9.16RR30 pKa = 11.84VAKK33 pKa = 10.16KK34 pKa = 9.3PAAKK38 pKa = 9.7RR39 pKa = 11.84VAKK42 pKa = 10.17KK43 pKa = 9.83AAPAAAPAQGASAQATPQKK62 pKa = 10.31SAAKK66 pKa = 9.94RR67 pKa = 11.84SVKK70 pKa = 10.21KK71 pKa = 10.68SAGKK75 pKa = 10.33RR76 pKa = 11.84SAKK79 pKa = 10.0KK80 pKa = 9.97SAGGKK85 pKa = 9.26KK86 pKa = 9.59RR87 pKa = 11.84ATKK90 pKa = 10.43

Molecular weight: 9.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20720

0

20720

11909904

49

21534

574.8

66.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.946 ± 0.012

1.168 ± 0.009

5.688 ± 0.009

6.5 ± 0.019

4.674 ± 0.014

4.158 ± 0.016

1.85 ± 0.007

7.619 ± 0.013

8.714 ± 0.022

8.786 ± 0.014

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.446 ± 0.007

7.549 ± 0.016

3.074 ± 0.012

8.363 ± 0.024

4.062 ± 0.011

7.923 ± 0.017

4.634 ± 0.017

4.426 ± 0.01

0.618 ± 0.005

3.801 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski