Amino acid dipepetide frequency for Stylonychia lemnae (Ciliate)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	1.965AlaAla: 1.965 ± 0.021	0.525AlaCys: 0.525 ± 0.007	1.933AlaAsp: 1.933 ± 0.014	2.182AlaGlu: 2.182 ± 0.016	2.059AlaPhe: 2.059 ± 0.015	1.793AlaGly: 1.793 ± 0.02	0.715AlaHis: 0.715 ± 0.009	3.115AlaIle: 3.115 ± 0.02	3.34AlaLys: 3.34 ± 0.018	3.811AlaLeu: 3.811 ± 0.018	0.953AlaMet: 0.953 ± 0.01	2.709AlaAsn: 2.709 ± 0.016	1.181AlaPro: 1.181 ± 0.015	3.003AlaGln: 3.003 ± 0.018	1.534AlaArg: 1.534 ± 0.012	3.092AlaSer: 3.092 ± 0.019	1.908AlaThr: 1.908 ± 0.016	1.925AlaVal: 1.925 ± 0.015	0.233AlaTrp: 0.233 ± 0.005	1.484AlaTyr: 1.484 ± 0.011	0.0AlaXaa: 0.0 ± 0.0
Cys	0.448CysAla: 0.448 ± 0.008	0.239CysCys: 0.239 ± 0.005	0.661CysAsp: 0.661 ± 0.01	0.649CysGlu: 0.649 ± 0.008	0.648CysPhe: 0.648 ± 0.008	0.629CysGly: 0.629 ± 0.01	0.225CysHis: 0.225 ± 0.005	0.861CysIle: 0.861 ± 0.009	0.896CysLys: 0.896 ± 0.011	1.21CysLeu: 1.21 ± 0.012	0.262CysMet: 0.262 ± 0.005	0.671CysAsn: 0.671 ± 0.008	0.492CysPro: 0.492 ± 0.025	0.835CysGln: 0.835 ± 0.009	0.411CysArg: 0.411 ± 0.006	0.883CysSer: 0.883 ± 0.011	0.54CysThr: 0.54 ± 0.011	0.513CysVal: 0.513 ± 0.007	0.101CysTrp: 0.101 ± 0.003	0.504CysTyr: 0.504 ± 0.007	0.0CysXaa: 0.0 ± 0.0
Asp	1.913AspAla: 1.913 ± 0.012	0.654AspCys: 0.654 ± 0.008	3.881AspAsp: 3.881 ± 0.022	4.508AspGlu: 4.508 ± 0.025	3.029AspPhe: 3.029 ± 0.017	2.3AspGly: 2.3 ± 0.022	1.092AspHis: 1.092 ± 0.011	4.312AspIle: 4.312 ± 0.022	4.676AspLys: 4.676 ± 0.022	5.342AspLeu: 5.342 ± 0.024	1.268AspMet: 1.268 ± 0.011	3.569AspAsn: 3.569 ± 0.016	1.871AspPro: 1.871 ± 0.014	4.832AspGln: 4.832 ± 0.023	2.061AspArg: 2.061 ± 0.016	4.232AspSer: 4.232 ± 0.019	2.141AspThr: 2.141 ± 0.015	2.273AspVal: 2.273 ± 0.016	0.403AspTrp: 0.403 ± 0.006	2.53AspTyr: 2.53 ± 0.018	0.0AspXaa: 0.0 ± 0.0
Glu	2.446GluAla: 2.446 ± 0.016	0.722GluCys: 0.722 ± 0.01	4.208GluAsp: 4.208 ± 0.022	6.05GluGlu: 6.05 ± 0.041	2.886GluPhe: 2.886 ± 0.018	2.33GluGly: 2.33 ± 0.017	0.964GluHis: 0.964 ± 0.009	5.342GluIle: 5.342 ± 0.024	6.178GluLys: 6.178 ± 0.032	5.511GluLeu: 5.511 ± 0.027	1.63GluMet: 1.63 ± 0.013	4.754GluAsn: 4.754 ± 0.023	1.303GluPro: 1.303 ± 0.011	4.945GluGln: 4.945 ± 0.028	2.94GluArg: 2.94 ± 0.02	4.435GluSer: 4.435 ± 0.022	2.685GluThr: 2.685 ± 0.016	2.883GluVal: 2.883 ± 0.016	0.415GluTrp: 0.415 ± 0.007	2.375GluTyr: 2.375 ± 0.015	0.0GluXaa: 0.0 ± 0.0
Phe	1.832PheAla: 1.832 ± 0.013	0.606PheCys: 0.606 ± 0.009	3.061PheAsp: 3.061 ± 0.019	3.143PheGlu: 3.143 ± 0.02	2.163PhePhe: 2.163 ± 0.019	2.343PheGly: 2.343 ± 0.018	0.805PheHis: 0.805 ± 0.009	3.501PheIle: 3.501 ± 0.023	4.094PheLys: 4.094 ± 0.019	3.92PheLeu: 3.92 ± 0.026	1.233PheMet: 1.233 ± 0.01	3.477PheAsn: 3.477 ± 0.017	1.156PhePro: 1.156 ± 0.011	3.399PheGln: 3.399 ± 0.018	1.716PheArg: 1.716 ± 0.015	3.645PheSer: 3.645 ± 0.021	2.266PheThr: 2.266 ± 0.018	2.073PheVal: 2.073 ± 0.017	0.34PheTrp: 0.34 ± 0.006	1.971PheTyr: 1.971 ± 0.016	0.0PheXaa: 0.0 ± 0.0
Gly	1.852GlyAla: 1.852 ± 0.021	0.555GlyCys: 0.555 ± 0.009	2.203GlyAsp: 2.203 ± 0.019	2.214GlyGlu: 2.214 ± 0.016	2.192GlyPhe: 2.192 ± 0.016	2.956GlyGly: 2.956 ± 0.035	0.793GlyHis: 0.793 ± 0.01	3.103GlyIle: 3.103 ± 0.018	3.214GlyLys: 3.214 ± 0.019	3.556GlyLeu: 3.556 ± 0.02	0.983GlyMet: 0.983 ± 0.012	2.782GlyAsn: 2.782 ± 0.019	1.028GlyPro: 1.028 ± 0.012	2.879GlyGln: 2.879 ± 0.021	1.574GlyArg: 1.574 ± 0.013	3.452GlySer: 3.452 ± 0.026	1.978GlyThr: 1.978 ± 0.02	2.136GlyVal: 2.136 ± 0.016	0.319GlyTrp: 0.319 ± 0.007	1.807GlyTyr: 1.807 ± 0.019	0.0GlyXaa: 0.0 ± 0.0
His	0.614HisAla: 0.614 ± 0.008	0.232HisCys: 0.232 ± 0.005	0.92HisAsp: 0.92 ± 0.01	1.053HisGlu: 1.053 ± 0.01	0.898HisPhe: 0.898 ± 0.008	0.734HisGly: 0.734 ± 0.011	0.623HisHis: 0.623 ± 0.009	1.258HisIle: 1.258 ± 0.012	1.46HisLys: 1.46 ± 0.012	1.771HisLeu: 1.771 ± 0.014	0.391HisMet: 0.391 ± 0.005	1.308HisAsn: 1.308 ± 0.011	0.692HisPro: 0.692 ± 0.007	1.86HisGln: 1.86 ± 0.015	0.808HisArg: 0.808 ± 0.009	1.562HisSer: 1.562 ± 0.012	0.753HisThr: 0.753 ± 0.008	0.653HisVal: 0.653 ± 0.008	0.121HisTrp: 0.121 ± 0.003	0.786HisTyr: 0.786 ± 0.007	0.0HisXaa: 0.0 ± 0.0
Ile	3.021IleAla: 3.021 ± 0.018	0.993IleCys: 0.993 ± 0.011	4.831IleAsp: 4.831 ± 0.02	5.185IleGlu: 5.185 ± 0.023	3.55IlePhe: 3.55 ± 0.025	3.052IleGly: 3.052 ± 0.02	1.353IleHis: 1.353 ± 0.011	5.617IleIle: 5.617 ± 0.029	6.865IleLys: 6.865 ± 0.03	6.593IleLeu: 6.593 ± 0.031	1.815IleMet: 1.815 ± 0.013	5.738IleAsn: 5.738 ± 0.026	2.423IlePro: 2.423 ± 0.016	6.15IleGln: 6.15 ± 0.029	2.915IleArg: 2.915 ± 0.016	5.861IleSer: 5.861 ± 0.028	3.396IleThr: 3.396 ± 0.022	3.459IleVal: 3.459 ± 0.02	0.492IleTrp: 0.492 ± 0.007	2.883IleTyr: 2.883 ± 0.02	0.0IleXaa: 0.0 ± 0.0
Lys	3.427LysAla: 3.427 ± 0.019	0.947LysCys: 0.947 ± 0.011	5.117LysAsp: 5.117 ± 0.026	6.079LysGlu: 6.079 ± 0.032	3.716LysPhe: 3.716 ± 0.019	2.998LysGly: 2.998 ± 0.018	1.561LysHis: 1.561 ± 0.013	6.919LysIle: 6.919 ± 0.029	8.411LysLys: 8.411 ± 0.052	7.697LysLeu: 7.697 ± 0.033	2.095LysMet: 2.095 ± 0.016	6.394LysAsn: 6.394 ± 0.031	2.422LysPro: 2.422 ± 0.017	7.073LysGln: 7.073 ± 0.036	3.918LysArg: 3.918 ± 0.027	6.601LysSer: 6.601 ± 0.032	3.975LysThr: 3.975 ± 0.021	4.016LysVal: 4.016 ± 0.018	0.508LysTrp: 0.508 ± 0.007	3.267LysTyr: 3.267 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	3.762LeuAla: 3.762 ± 0.018	1.017LeuCys: 1.017 ± 0.011	5.071LeuAsp: 5.071 ± 0.022	5.829LeuGlu: 5.829 ± 0.028	3.841LeuPhe: 3.841 ± 0.024	3.61LeuGly: 3.61 ± 0.018	1.473LeuHis: 1.473 ± 0.013	6.751LeuIle: 6.751 ± 0.032	8.482LeuLys: 8.482 ± 0.037	7.324LeuLeu: 7.324 ± 0.038	2.332LeuMet: 2.332 ± 0.016	7.015LeuAsn: 7.015 ± 0.029	2.647LeuPro: 2.647 ± 0.018	6.432LeuGln: 6.432 ± 0.028	3.989LeuArg: 3.989 ± 0.023	6.784LeuSer: 6.784 ± 0.03	4.12LeuThr: 4.12 ± 0.023	3.921LeuVal: 3.921 ± 0.019	0.52LeuTrp: 0.52 ± 0.007	2.945LeuTyr: 2.945 ± 0.019	0.0LeuXaa: 0.0 ± 0.0
Met	1.012MetAla: 1.012 ± 0.009	0.225MetCys: 0.225 ± 0.004	1.227MetAsp: 1.227 ± 0.011	1.42MetGlu: 1.42 ± 0.013	0.968MetPhe: 0.968 ± 0.01	0.994MetGly: 0.994 ± 0.011	0.457MetHis: 0.457 ± 0.007	2.222MetIle: 2.222 ± 0.015	2.346MetLys: 2.346 ± 0.017	1.99MetLeu: 1.99 ± 0.014	0.837MetMet: 0.837 ± 0.011	2.085MetAsn: 2.085 ± 0.014	0.801MetPro: 0.801 ± 0.008	1.642MetGln: 1.642 ± 0.014	1.233MetArg: 1.233 ± 0.012	1.89MetSer: 1.89 ± 0.013	1.285MetThr: 1.285 ± 0.009	1.007MetVal: 1.007 ± 0.01	0.134MetTrp: 0.134 ± 0.004	0.689MetTyr: 0.689 ± 0.007	0.0MetXaa: 0.0 ± 0.0
Asn	2.602AsnAla: 2.602 ± 0.016	0.787AsnCys: 0.787 ± 0.012	4.004AsnAsp: 4.004 ± 0.017	4.666AsnGlu: 4.666 ± 0.022	3.481AsnPhe: 3.481 ± 0.02	2.905AsnGly: 2.905 ± 0.023	1.635AsnHis: 1.635 ± 0.014	5.347AsnIle: 5.347 ± 0.029	5.946AsnLys: 5.946 ± 0.029	6.939AsnLeu: 6.939 ± 0.029	1.723AsnMet: 1.723 ± 0.013	6.344AsnAsn: 6.344 ± 0.038	2.67AsnPro: 2.67 ± 0.018	7.68AsnGln: 7.68 ± 0.034	2.668AsnArg: 2.668 ± 0.018	6.384AsnSer: 6.384 ± 0.032	3.475AsnThr: 3.475 ± 0.018	2.822AsnVal: 2.822 ± 0.017	0.387AsnTrp: 0.387 ± 0.006	3.027AsnTyr: 3.027 ± 0.016	0.0AsnXaa: 0.0 ± 0.0
Pro	1.203ProAla: 1.203 ± 0.015	0.29ProCys: 0.29 ± 0.006	1.577ProAsp: 1.577 ± 0.012	1.865ProGlu: 1.865 ± 0.015	1.462ProPhe: 1.462 ± 0.013	1.234ProGly: 1.234 ± 0.02	0.573ProHis: 0.573 ± 0.008	2.339ProIle: 2.339 ± 0.015	2.566ProLys: 2.566 ± 0.014	2.669ProLeu: 2.669 ± 0.017	0.673ProMet: 0.673 ± 0.009	2.235ProAsn: 2.235 ± 0.017	1.278ProPro: 1.278 ± 0.021	2.64ProGln: 2.64 ± 0.018	1.176ProArg: 1.176 ± 0.012	2.584ProSer: 2.584 ± 0.021	1.594ProThr: 1.594 ± 0.017	1.432ProVal: 1.432 ± 0.014	0.184ProTrp: 0.184 ± 0.004	1.167ProTyr: 1.167 ± 0.013	0.0ProXaa: 0.0 ± 0.0
Gln	3.123GlnAla: 3.123 ± 0.018	0.773GlnCys: 0.773 ± 0.012	4.243GlnAsp: 4.243 ± 0.022	5.17GlnGlu: 5.17 ± 0.028	3.251GlnPhe: 3.251 ± 0.018	2.709GlnGly: 2.709 ± 0.014	1.389GlnHis: 1.389 ± 0.013	6.57GlnIle: 6.57 ± 0.031	7.291GlnLys: 7.291 ± 0.036	6.766GlnLeu: 6.766 ± 0.033	2.257GlnMet: 2.257 ± 0.016	7.429GlnAsn: 7.429 ± 0.037	2.299GlnPro: 2.299 ± 0.017	8.943GlnGln: 8.943 ± 0.075	3.538GlnArg: 3.538 ± 0.021	7.069GlnSer: 7.069 ± 0.04	4.043GlnThr: 4.043 ± 0.02	3.543GlnVal: 3.543 ± 0.019	0.403GlnTrp: 0.403 ± 0.007	2.818GlnTyr: 2.818 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	1.648ArgAla: 1.648 ± 0.013	0.413ArgCys: 0.413 ± 0.007	2.521ArgAsp: 2.521 ± 0.017	2.821ArgGlu: 2.821 ± 0.021	1.81ArgPhe: 1.81 ± 0.012	1.735ArgGly: 1.735 ± 0.016	0.704ArgHis: 0.704 ± 0.007	2.988ArgIle: 2.988 ± 0.018	3.74ArgLys: 3.74 ± 0.024	3.524ArgLeu: 3.524 ± 0.019	1.018ArgMet: 1.018 ± 0.01	2.966ArgAsn: 2.966 ± 0.017	1.2ArgPro: 1.2 ± 0.009	3.241ArgGln: 3.241 ± 0.022	2.021ArgArg: 2.021 ± 0.015	2.917ArgSer: 2.917 ± 0.021	1.669ArgThr: 1.669 ± 0.012	1.992ArgVal: 1.992 ± 0.013	0.233ArgTrp: 0.233 ± 0.005	1.457ArgTyr: 1.457 ± 0.012	0.0ArgXaa: 0.0 ± 0.0
Ser	2.991SerAla: 2.991 ± 0.02	0.86SerCys: 0.86 ± 0.013	3.88SerAsp: 3.88 ± 0.02	4.075SerGlu: 4.075 ± 0.021	3.746SerPhe: 3.746 ± 0.021	3.248SerGly: 3.248 ± 0.022	1.549SerHis: 1.549 ± 0.013	5.884SerIle: 5.884 ± 0.026	6.789SerLys: 6.789 ± 0.033	6.797SerLeu: 6.797 ± 0.027	1.865SerMet: 1.865 ± 0.012	6.415SerAsn: 6.415 ± 0.031	2.855SerPro: 2.855 ± 0.02	7.256SerGln: 7.256 ± 0.04	3.152SerArg: 3.152 ± 0.022	7.383SerSer: 7.383 ± 0.04	3.975SerThr: 3.975 ± 0.027	3.327SerVal: 3.327 ± 0.018	0.418SerTrp: 0.418 ± 0.007	2.767SerTyr: 2.767 ± 0.019	0.0SerXaa: 0.0 ± 0.0
Thr	1.975ThrAla: 1.975 ± 0.018	0.601ThrCys: 0.601 ± 0.012	2.258ThrAsp: 2.258 ± 0.014	2.434ThrGlu: 2.434 ± 0.013	2.403ThrPhe: 2.403 ± 0.019	2.046ThrGly: 2.046 ± 0.02	0.873ThrHis: 0.873 ± 0.008	3.53ThrIle: 3.53 ± 0.02	3.586ThrLys: 3.586 ± 0.021	4.317ThrLeu: 4.317 ± 0.023	1.018ThrMet: 1.018 ± 0.011	3.45ThrAsn: 3.45 ± 0.02	1.786ThrPro: 1.786 ± 0.016	3.887ThrGln: 3.887 ± 0.023	1.587ThrArg: 1.587 ± 0.013	3.816ThrSer: 3.816 ± 0.028	2.58ThrThr: 2.58 ± 0.031	2.118ThrVal: 2.118 ± 0.017	0.28ThrTrp: 0.28 ± 0.006	1.795ThrTyr: 1.795 ± 0.016	0.0ThrXaa: 0.0 ± 0.0
Val	1.927ValAla: 1.927 ± 0.016	0.581ValCys: 0.581 ± 0.008	2.583ValAsp: 2.583 ± 0.015	2.823ValGlu: 2.823 ± 0.016	2.243ValPhe: 2.243 ± 0.017	1.944ValGly: 1.944 ± 0.016	0.791ValHis: 0.791 ± 0.008	3.498ValIle: 3.498 ± 0.019	3.901ValLys: 3.901 ± 0.019	3.803ValLeu: 3.803 ± 0.022	1.063ValMet: 1.063 ± 0.01	3.124ValAsn: 3.124 ± 0.016	1.379ValPro: 1.379 ± 0.013	3.244ValGln: 3.244 ± 0.018	1.693ValArg: 1.693 ± 0.013	3.325ValSer: 3.325 ± 0.018	2.049ValThr: 2.049 ± 0.018	2.234ValVal: 2.234 ± 0.024	0.333ValTrp: 0.333 ± 0.005	1.726ValTyr: 1.726 ± 0.013	0.0ValXaa: 0.0 ± 0.0
Trp	0.295TrpAla: 0.295 ± 0.006	0.081TrpCys: 0.081 ± 0.002	0.377TrpAsp: 0.377 ± 0.007	0.352TrpGlu: 0.352 ± 0.005	0.298TrpPhe: 0.298 ± 0.005	0.291TrpGly: 0.291 ± 0.006	0.117TrpHis: 0.117 ± 0.003	0.522TrpIle: 0.522 ± 0.007	0.515TrpLys: 0.515 ± 0.007	0.525TrpLeu: 0.525 ± 0.008	0.164TrpMet: 0.164 ± 0.004	0.451TrpAsn: 0.451 ± 0.006	0.168TrpPro: 0.168 ± 0.004	0.338TrpGln: 0.338 ± 0.006	0.301TrpArg: 0.301 ± 0.006	0.44TrpSer: 0.44 ± 0.008	0.316TrpThr: 0.316 ± 0.006	0.34TrpVal: 0.34 ± 0.005	0.06TrpTrp: 0.06 ± 0.003	0.23TrpTyr: 0.23 ± 0.004	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.407TyrAla: 1.407 ± 0.013	0.576TyrCys: 0.576 ± 0.013	2.329TyrAsp: 2.329 ± 0.017	2.483TyrGlu: 2.483 ± 0.016	2.099TyrPhe: 2.099 ± 0.016	1.725TyrGly: 1.725 ± 0.016	0.804TyrHis: 0.804 ± 0.009	2.511TyrIle: 2.511 ± 0.017	2.844TyrLys: 2.844 ± 0.017	3.802TyrLeu: 3.802 ± 0.021	0.883TyrMet: 0.883 ± 0.009	2.577TyrAsn: 2.577 ± 0.016	1.091TyrPro: 1.091 ± 0.013	3.348TyrGln: 3.348 ± 0.017	1.385TyrArg: 1.385 ± 0.013	2.879TyrSer: 2.879 ± 0.022	1.593TyrThr: 1.593 ± 0.015	1.595TyrVal: 1.595 ± 0.013	0.297TyrTrp: 0.297 ± 0.005	1.779TyrTyr: 1.779 ± 0.013	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 20720 proteins (11909904 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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