Devosia sp. Root685
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4002 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0Q8ICC0|A0A0Q8ICC0_9RHIZ NMT1 domain-containing protein OS=Devosia sp. Root685 OX=1736587 GN=ASD83_20100 PE=4 SV=1
MM1 pKa = 6.74 EE2 pKa = 4.39 TAATTIAGYY11 pKa = 10.72 ALYY14 pKa = 10.54 GRR16 pKa = 11.84 LEE18 pKa = 4.03 GDD20 pKa = 3.32 VFYY23 pKa = 10.38 FALSSAQPINATSTLWLNTDD43 pKa = 3.36 ANINTGYY50 pKa = 10.31 KK51 pKa = 9.36 VWGFAAGAEE60 pKa = 4.29 FNVNFGANGVPRR72 pKa = 11.84 LYY74 pKa = 10.4 TGADD78 pKa = 3.29 GQTLVGDD85 pKa = 4.17 LTYY88 pKa = 11.57 SMSADD93 pKa = 3.12 RR94 pKa = 11.84 KK95 pKa = 8.61 VLEE98 pKa = 3.97 IALPKK103 pKa = 10.31 SLLGASVSSVGIMADD118 pKa = 2.97 INNTIFLPADD128 pKa = 3.54 YY129 pKa = 8.78 TQAAYY134 pKa = 9.08 TITTPVPSVYY144 pKa = 10.5 DD145 pKa = 3.52 GLLTEE150 pKa = 4.32 WTVEE154 pKa = 3.75 QRR156 pKa = 11.84 LDD158 pKa = 3.45 NTPDD162 pKa = 3.33 KK163 pKa = 11.18 VVAGYY168 pKa = 9.93 EE169 pKa = 4.17 LYY171 pKa = 11.12 GKK173 pKa = 10.06 VADD176 pKa = 4.36 DD177 pKa = 3.93 SFVFGLKK184 pKa = 10.06 SAVAIGPNTTFWLNTDD200 pKa = 3.23 NNTATGYY207 pKa = 9.48 QIWGFAGGAEE217 pKa = 4.12 FNVNIGADD225 pKa = 3.78 GVARR229 pKa = 11.84 LYY231 pKa = 11.07 SGADD235 pKa = 3.21 GQTLVGTLEE244 pKa = 3.98 YY245 pKa = 10.42 KK246 pKa = 10.35 IAAGGLSMEE255 pKa = 4.35 FAVPKK260 pKa = 10.5 ALLGPTVTSVVVLADD275 pKa = 3.28 INNSVFLPPSYY286 pKa = 11.04 SSGGYY291 pKa = 9.96 VLTTPLVVPPGPYY304 pKa = 9.99 DD305 pKa = 3.58 GLLTEE310 pKa = 4.51 WTAAQRR316 pKa = 11.84 LDD318 pKa = 3.41 SGAGVVAGYY327 pKa = 9.72 EE328 pKa = 4.01 LYY330 pKa = 9.64 GTVSEE335 pKa = 4.81 DD336 pKa = 3.14 KK337 pKa = 10.84 FVFALKK343 pKa = 10.3 SAVSIGQNTTFWLNTDD359 pKa = 3.51 ANVATGHH366 pKa = 6.04 QIFGFAGGAEE376 pKa = 4.11 FNVNIGADD384 pKa = 3.78 GVARR388 pKa = 11.84 LYY390 pKa = 11.07 SGADD394 pKa = 3.2 GQTLVGEE401 pKa = 4.58 IDD403 pKa = 3.92 HH404 pKa = 7.05 KK405 pKa = 10.66 IAPDD409 pKa = 3.51 GMSMEE414 pKa = 4.33 FAVPRR419 pKa = 11.84 SLIGASVTAITLLADD434 pKa = 3.54 VNDD437 pKa = 4.28 TVFLPTTYY445 pKa = 11.35 ANGGYY450 pKa = 10.8 ALVDD454 pKa = 3.71 PASIPPSQFDD464 pKa = 3.57 GVLTEE469 pKa = 4.05 WTANQRR475 pKa = 11.84 LEE477 pKa = 4.21 TPMTSVDD484 pKa = 3.21 GYY486 pKa = 10.63 EE487 pKa = 4.62 FYY489 pKa = 11.16 GQYY492 pKa = 10.67 SDD494 pKa = 3.92 GQFTFGFKK502 pKa = 10.12 SAVVIGPNTTFWLNTDD518 pKa = 3.58 GNVATGTQVFGYY530 pKa = 10.77 AGGAEE535 pKa = 4.14 FNVNIGSDD543 pKa = 3.74 GVARR547 pKa = 11.84 LYY549 pKa = 11.04 SGAAGQTLVGAIDD562 pKa = 3.88 YY563 pKa = 11.08 KK564 pKa = 10.92 LGPDD568 pKa = 3.25 GKK570 pKa = 8.48 TIEE573 pKa = 4.27 FAVPKK578 pKa = 10.55 AMIGASVTSVSILADD593 pKa = 3.51 VNDD596 pKa = 4.5 SVFLPANYY604 pKa = 10.03 LSPAYY609 pKa = 8.84 TVYY612 pKa = 11.03 DD613 pKa = 4.08 PASLPAVTDD622 pKa = 3.37 TGNKK626 pKa = 7.9 IAIVFSQTTANNYY639 pKa = 8.38 FSQMAYY645 pKa = 9.89 SQLVMAAQSQAMAAGIPFDD664 pKa = 4.42 LVSEE668 pKa = 4.52 ADD670 pKa = 3.81 LADD673 pKa = 3.78 LSKK676 pKa = 10.4 MVNYY680 pKa = 10.04 DD681 pKa = 3.76 AIVFPSFRR689 pKa = 11.84 NVPANYY695 pKa = 8.58 TAIYY699 pKa = 10.37 DD700 pKa = 3.83 VLTKK704 pKa = 10.52 LVYY707 pKa = 10.39 QYY709 pKa = 11.33 DD710 pKa = 3.69 VSLIAAGDD718 pKa = 3.8 FMTNDD723 pKa = 3.86 ANNASLPNNAYY734 pKa = 10.23 EE735 pKa = 4.73 RR736 pKa = 11.84 MQTLFGLNRR745 pKa = 11.84 TGGEE749 pKa = 4.03 DD750 pKa = 3.46 GVTVNIEE757 pKa = 3.82 ATPAGHH763 pKa = 7.81 AITEE767 pKa = 4.59 GYY769 pKa = 8.87 GANGAIHH776 pKa = 7.48 TYY778 pKa = 9.48 TGAATSYY785 pKa = 10.54 FSAANPNAGSVTVIAEE801 pKa = 3.95 QVVNGTTHH809 pKa = 6.09 SAVLGTVTGGRR820 pKa = 11.84 NVHH823 pKa = 6.53 FSTDD827 pKa = 3.21 ALLGDD832 pKa = 4.88 LNLLGQAIDD841 pKa = 3.63 WVNEE845 pKa = 3.94 EE846 pKa = 4.24 TGGPTVSLNMTRR858 pKa = 11.84 NTSLFASRR866 pKa = 11.84 NDD868 pKa = 3.1 MDD870 pKa = 4.37 QSQEE874 pKa = 4.08 TYY876 pKa = 10.84 DD877 pKa = 3.77 VDD879 pKa = 3.68 GGIYY883 pKa = 10.47 DD884 pKa = 3.66 AMLPFLQQWKK894 pKa = 8.25 TDD896 pKa = 3.62 YY897 pKa = 11.18 NFVGSYY903 pKa = 8.66 YY904 pKa = 10.99 VNVGFNPPDD913 pKa = 3.39 QEE915 pKa = 4.7 TNWLISSQYY924 pKa = 8.04 YY925 pKa = 10.51 DD926 pKa = 3.58 AMRR929 pKa = 11.84 AMGNEE934 pKa = 4.21 IGSHH938 pKa = 6.11 SYY940 pKa = 7.77 THH942 pKa = 7.36 PEE944 pKa = 3.83 DD945 pKa = 4.05 TNFLLPNVLTQALLDD960 pKa = 3.75 QRR962 pKa = 11.84 IAQYY966 pKa = 9.02 ATRR969 pKa = 11.84 PGGPGIIGQSLASMSLADD987 pKa = 4.1 INAKK991 pKa = 9.44 IAQVLAAPNPSALDD1005 pKa = 3.56 ATSKK1009 pKa = 11.14 AFLEE1013 pKa = 4.18 ATYY1016 pKa = 9.83 TFQFATARR1024 pKa = 11.84 AVLEE1028 pKa = 4.35 ANLGYY1033 pKa = 10.42 SIGGAAVPGMPEE1045 pKa = 3.7 QLLPAQQIIQYY1056 pKa = 10.01 YY1057 pKa = 10.13 DD1058 pKa = 3.64 YY1059 pKa = 11.15 LSGGASMLGAGYY1071 pKa = 9.27 PGAIGYY1077 pKa = 7.53 LTPGADD1083 pKa = 3.4 GQVYY1087 pKa = 10.01 IAPNMSFDD1095 pKa = 3.77 FTLMGWLGLTVAQAEE1110 pKa = 4.55 AKK1112 pKa = 8.64 WAAEE1116 pKa = 3.88 WSQLTANSDD1125 pKa = 3.46 MPIVVWPWHH1134 pKa = 6.95 DD1135 pKa = 3.69 YY1136 pKa = 11.13 GVTSWSLDD1144 pKa = 3.09 AGQPSPYY1151 pKa = 8.03 TAAMFTNFIAAAYY1164 pKa = 8.88 AAGTEE1169 pKa = 4.34 FVTLADD1175 pKa = 3.8 LAARR1179 pKa = 11.84 IKK1181 pKa = 10.79 AFEE1184 pKa = 3.99 RR1185 pKa = 11.84 ADD1187 pKa = 3.62 FGFSVAGDD1195 pKa = 4.48 VITMHH1200 pKa = 7.05 ATPQQGQLGTFALNLDD1216 pKa = 3.95 DD1217 pKa = 6.61 LGGKK1221 pKa = 7.01 TIKK1224 pKa = 10.56 SVANWYY1230 pKa = 10.43 AYY1232 pKa = 10.39 DD1233 pKa = 4.22 ADD1235 pKa = 4.88 SVFLDD1240 pKa = 3.62 ADD1242 pKa = 3.66 GGTFTVQLGTTVDD1255 pKa = 4.05 DD1256 pKa = 3.93 VTHH1259 pKa = 5.94 ITSIGSRR1266 pKa = 11.84 AQLVSLTGDD1275 pKa = 3.4 GTNLDD1280 pKa = 3.59 FTIAGEE1286 pKa = 4.36 GKK1288 pKa = 8.55 VTIDD1292 pKa = 3.36 VKK1294 pKa = 9.83 QTAGFVYY1301 pKa = 9.9 QATGATIVSQTGDD1314 pKa = 3.56 ILTLDD1319 pKa = 4.01 LGAIGSHH1326 pKa = 4.7 TVALRR1331 pKa = 11.84 QVAINKK1337 pKa = 9.54 APTDD1341 pKa = 3.54 IVVSNQVALPEE1352 pKa = 3.93 NTAARR1357 pKa = 11.84 TKK1359 pKa = 10.4 IADD1362 pKa = 4.13 LAVIDD1367 pKa = 5.22 PDD1369 pKa = 3.8 TDD1371 pKa = 3.46 PLLRR1375 pKa = 11.84 MNVVTLSDD1383 pKa = 3.19 ARR1385 pKa = 11.84 FEE1387 pKa = 4.39 VDD1389 pKa = 3.03 ASTGALYY1396 pKa = 10.7 LKK1398 pKa = 10.45 AGQVVNFEE1406 pKa = 4.34 AGATIAMTLTATDD1419 pKa = 4.53 GPLSFSKK1426 pKa = 10.9 ALVLTVVNVNEE1437 pKa = 4.65 APTGAPTVAGAAVEE1451 pKa = 4.13 NVLLSSGTALIADD1464 pKa = 4.42 PDD1466 pKa = 3.98 GLGAFTYY1473 pKa = 9.55 QWQRR1477 pKa = 11.84 GNGTVFTNIAGATAATYY1494 pKa = 10.85 RR1495 pKa = 11.84 LVQADD1500 pKa = 3.68 ADD1502 pKa = 3.81 QLVRR1506 pKa = 11.84 VAVSYY1511 pKa = 11.08 RR1512 pKa = 11.84 DD1513 pKa = 3.48 GGATTEE1519 pKa = 4.58 TVFSAATTQVVDD1531 pKa = 3.61 VLANTTLTPLAANTSRR1547 pKa = 11.84 TITTAEE1553 pKa = 3.92 LVGTGISGTVTINSLTASIGTLVALGGGQWTYY1585 pKa = 10.83 TPPLNNDD1592 pKa = 3.39 TAVTFSYY1599 pKa = 9.6 TASNGTKK1606 pKa = 10.29 LAQGTSAMDD1615 pKa = 4.18 LLPSNSIMGTAGVDD1629 pKa = 3.23 ILAGRR1634 pKa = 11.84 ASADD1638 pKa = 3.72 TYY1640 pKa = 11.11 HH1641 pKa = 6.9 GLAGGDD1647 pKa = 4.0 TISGGAGDD1655 pKa = 4.96 DD1656 pKa = 3.84 IIYY1659 pKa = 10.65 GDD1661 pKa = 4.58 EE1662 pKa = 4.69 GNDD1665 pKa = 3.46 TANGGDD1671 pKa = 3.73 GNDD1674 pKa = 3.3 VFFATLNDD1682 pKa = 3.6 GNDD1685 pKa = 3.41 RR1686 pKa = 11.84 YY1687 pKa = 8.97 TGNAGSDD1694 pKa = 3.5 RR1695 pKa = 11.84 YY1696 pKa = 10.66 DD1697 pKa = 3.62 LSGTTAAATVNLTNGTATSSQTGSDD1722 pKa = 3.2 TLATIEE1728 pKa = 4.32 NVTGGSGADD1737 pKa = 3.61 TITGSSAANVLNGGAGNDD1755 pKa = 3.28 ILLGMGGADD1764 pKa = 3.52 TLIGGDD1770 pKa = 3.42 GADD1773 pKa = 4.08 RR1774 pKa = 11.84 LTGGAGRR1781 pKa = 11.84 DD1782 pKa = 3.81 VMTGGADD1789 pKa = 3.18 NDD1791 pKa = 4.21 TFIFTSTAEE1800 pKa = 4.07 MGTTATTRR1808 pKa = 11.84 DD1809 pKa = 3.9 VITDD1813 pKa = 3.76 FTPGQDD1819 pKa = 3.25 KK1820 pKa = 10.96 FDD1822 pKa = 4.55 FSAIDD1827 pKa = 3.71 ANTMLSGNQAFTFLATQGAAFTGVRR1852 pKa = 11.84 GQLNWSVQDD1861 pKa = 3.76 AAGTANDD1868 pKa = 3.69 KK1869 pKa = 10.29 TIVMGDD1875 pKa = 3.51 LNGDD1879 pKa = 4.39 RR1880 pKa = 11.84 IADD1883 pKa = 3.47 FHH1885 pKa = 8.43 VEE1887 pKa = 3.93 LTGLIQLGVGDD1898 pKa = 5.52 FILL1901 pKa = 5.14
Molecular weight: 197.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.753
IPC2_protein 3.91
IPC_protein 3.973
Toseland 3.732
ProMoST 4.126
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.91
Rodwell 3.783
Grimsley 3.643
Solomon 3.961
Lehninger 3.923
Nozaki 4.062
DTASelect 4.355
Thurlkill 3.783
EMBOSS 3.91
Sillero 4.088
Patrickios 1.939
IPC_peptide 3.961
IPC2_peptide 4.062
IPC2.peptide.svr19 3.973
Protein with the highest isoelectric point:
>tr|A0A0Q8IC93|A0A0Q8IC93_9RHIZ Copper homeostasis protein CutC OS=Devosia sp. Root685 OX=1736587 GN=cutC PE=3 SV=1
MM1 pKa = 7.02 VPWRR5 pKa = 11.84 KK6 pKa = 7.27 TLRR9 pKa = 11.84 RR10 pKa = 11.84 LDD12 pKa = 3.59 GAYY15 pKa = 10.0 SEE17 pKa = 4.53 HH18 pKa = 6.23 TLRR21 pKa = 11.84 SYY23 pKa = 11.14 RR24 pKa = 11.84 SDD26 pKa = 3.09 FGAFLRR32 pKa = 11.84 WCRR35 pKa = 11.84 KK36 pKa = 9.09 KK37 pKa = 10.55 RR38 pKa = 11.84 LKK40 pKa = 10.49 ALPAASTTIVAYY52 pKa = 9.78 IDD54 pKa = 3.57 AEE56 pKa = 4.23 GMRR59 pKa = 11.84 LKK61 pKa = 10.68 PSTVKK66 pKa = 10.35 RR67 pKa = 11.84 RR68 pKa = 11.84 LCALRR73 pKa = 11.84 RR74 pKa = 11.84 IHH76 pKa = 6.72 HH77 pKa = 6.71 LSDD80 pKa = 4.47 LGDD83 pKa = 3.64 PTQSEE88 pKa = 4.76 EE89 pKa = 3.83 VQLAMRR95 pKa = 11.84 RR96 pKa = 11.84 VRR98 pKa = 11.84 RR99 pKa = 11.84 AQPSRR104 pKa = 11.84 PKK106 pKa = 9.57 QAHH109 pKa = 5.93 GVTASLRR116 pKa = 11.84 DD117 pKa = 3.43 QLLAVCGDD125 pKa = 3.92 DD126 pKa = 5.68 LIGIRR131 pKa = 11.84 DD132 pKa = 3.71 KK133 pKa = 11.6 VLVSVGFDD141 pKa = 3.19 TLCRR145 pKa = 11.84 RR146 pKa = 11.84 GEE148 pKa = 3.99 LVALSIEE155 pKa = 4.51 DD156 pKa = 4.41 FSPTSDD162 pKa = 2.95 GRR164 pKa = 11.84 HH165 pKa = 4.09 TVLVRR170 pKa = 11.84 RR171 pKa = 11.84 AKK173 pKa = 10.44 NDD175 pKa = 3.41 PEE177 pKa = 4.57 GAGRR181 pKa = 11.84 TAKK184 pKa = 10.27 LSKK187 pKa = 10.47 ASSDD191 pKa = 4.69 VFRR194 pKa = 11.84 QWLKK198 pKa = 9.82 EE199 pKa = 3.63 AGIYY203 pKa = 9.81 RR204 pKa = 11.84 GPLLRR209 pKa = 11.84 PVYY212 pKa = 9.99 GNRR215 pKa = 11.84 VRR217 pKa = 11.84 ALYY220 pKa = 10.44 LEE222 pKa = 4.68 PLTVGRR228 pKa = 11.84 VLKK231 pKa = 10.26 KK232 pKa = 10.33 LCDD235 pKa = 3.6 RR236 pKa = 11.84 AGLEE240 pKa = 3.86 ALTTEE245 pKa = 4.44 RR246 pKa = 11.84 VSGHH250 pKa = 5.29 SLRR253 pKa = 11.84 VGAAQQLTINGFGLPQIMRR272 pKa = 11.84 AGGWKK277 pKa = 9.75 SVNVVARR284 pKa = 11.84 YY285 pKa = 9.44 IEE287 pKa = 4.62 NVDD290 pKa = 3.59 LDD292 pKa = 3.61 VWKK295 pKa = 10.81
Molecular weight: 33.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 9.589
IPC_protein 10.35
Toseland 10.672
ProMoST 10.379
Dawson 10.76
Bjellqvist 10.467
Wikipedia 10.965
Rodwell 10.965
Grimsley 10.804
Solomon 10.877
Lehninger 10.833
Nozaki 10.657
DTASelect 10.452
Thurlkill 10.657
EMBOSS 11.067
Sillero 10.687
Patrickios 10.657
IPC_peptide 10.877
IPC2_peptide 9.487
IPC2.peptide.svr19 8.678
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4002
0
4002
1280198
41
4685
319.9
34.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.053 ± 0.05
0.641 ± 0.01
5.698 ± 0.032
5.673 ± 0.04
3.967 ± 0.024
8.609 ± 0.045
1.979 ± 0.019
5.596 ± 0.027
3.268 ± 0.031
10.244 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.539 ± 0.019
2.915 ± 0.029
4.992 ± 0.027
3.183 ± 0.02
6.279 ± 0.044
5.515 ± 0.027
5.558 ± 0.035
7.638 ± 0.032
1.343 ± 0.017
2.308 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here