Bat polyomavirus 5b

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; Alphapolyomavirus; Acerodon celebensis polyomavirus 1

Average proteome isoelectric point is 5.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D5ZZ19|A0A0D5ZZ19_9POLY Capsid protein VP1 OS=Bat polyomavirus 5b OX=1623684 GN=VP1 PE=3 SV=1
MM1 pKa = 7.54GGFISLLLNLAEE13 pKa = 4.86ALPEE17 pKa = 4.02LSAATGFSVEE27 pKa = 4.58AIISGEE33 pKa = 3.5AAAAVEE39 pKa = 5.61AEE41 pKa = 4.45ASWLMSSTGLALTAAEE57 pKa = 4.22ALEE60 pKa = 4.11ATGISAEE67 pKa = 4.29SISMLFAVPSALRR80 pKa = 11.84DD81 pKa = 3.66LVGLGITFQTVTGVSALVAAGIRR104 pKa = 11.84IEE106 pKa = 3.9QHH108 pKa = 4.27QVSVVNQNSMALSIWRR124 pKa = 11.84PQDD127 pKa = 3.2YY128 pKa = 11.08YY129 pKa = 11.42DD130 pKa = 3.73ILFPGVNSFVQAINVVEE147 pKa = 4.08NWGSSLFNYY156 pKa = 8.65VGRR159 pKa = 11.84YY160 pKa = 7.97IWDD163 pKa = 3.5SLVRR167 pKa = 11.84EE168 pKa = 4.24ARR170 pKa = 11.84LQIGQATNEE179 pKa = 4.04LAVRR183 pKa = 11.84AHH185 pKa = 6.52HH186 pKa = 6.33SFHH189 pKa = 7.63DD190 pKa = 3.59ALARR194 pKa = 11.84VAEE197 pKa = 4.24NTRR200 pKa = 3.3

Molecular weight: 21.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D5ZYL4|A0A0D5ZYL4_9POLY Large T antigen OS=Bat polyomavirus 5b OX=1623684 PE=4 SV=1
MM1 pKa = 8.04DD2 pKa = 4.58KK3 pKa = 11.02FMDD6 pKa = 3.96RR7 pKa = 11.84DD8 pKa = 3.62EE9 pKa = 5.31LKK11 pKa = 10.5EE12 pKa = 4.04LCEE15 pKa = 3.99LLQIPAHH22 pKa = 6.63CYY24 pKa = 10.71GNLPMMKK31 pKa = 9.63INYY34 pKa = 9.7KK35 pKa = 10.27KK36 pKa = 9.78MNKK39 pKa = 9.08IYY41 pKa = 10.64HH42 pKa = 6.63PDD44 pKa = 3.32KK45 pKa = 11.45GGDD48 pKa = 3.47AEE50 pKa = 4.41KK51 pKa = 9.82MKK53 pKa = 10.79RR54 pKa = 11.84MNEE57 pKa = 3.67LWQKK61 pKa = 10.48LQDD64 pKa = 4.0GVCNARR70 pKa = 11.84DD71 pKa = 3.78EE72 pKa = 5.02GPVSRR77 pKa = 11.84WFWEE81 pKa = 4.29YY82 pKa = 10.95EE83 pKa = 3.74AMTLSEE89 pKa = 4.51FLGPDD94 pKa = 3.55FQTRR98 pKa = 11.84FCKK101 pKa = 10.42VYY103 pKa = 9.12PCCAFTPKK111 pKa = 9.49EE112 pKa = 3.76FCMCVCCLLNKK123 pKa = 7.21QHH125 pKa = 7.5RR126 pKa = 11.84IYY128 pKa = 10.57KK129 pKa = 8.84VKK131 pKa = 10.54KK132 pKa = 9.09EE133 pKa = 4.11KK134 pKa = 10.44KK135 pKa = 8.05CLVWGDD141 pKa = 3.73CFCYY145 pKa = 9.76RR146 pKa = 11.84CYY148 pKa = 10.88LLWFGFPHH156 pKa = 7.08NEE158 pKa = 4.5EE159 pKa = 4.38SFHH162 pKa = 4.69WWAMILAEE170 pKa = 4.73TEE172 pKa = 4.23LKK174 pKa = 10.61LLNLFNPGAWGKK186 pKa = 9.8YY187 pKa = 6.99VYY189 pKa = 10.63GVGNCYY195 pKa = 10.12IAMHH199 pKa = 6.7

Molecular weight: 23.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1411

199

634

352.8

39.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.733 ± 1.921

2.764 ± 0.869

5.315 ± 0.83

6.237 ± 0.393

4.465 ± 0.627

6.662 ± 1.236

1.984 ± 0.314

4.607 ± 0.482

7.371 ± 1.718

9.497 ± 0.436

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.26 ± 0.424

5.174 ± 0.242

5.67 ± 1.052

4.111 ± 0.443

3.26 ± 0.309

5.599 ± 1.094

5.315 ± 0.935

6.804 ± 0.708

1.347 ± 0.592

3.827 ± 0.447
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski