Falsochrobactrum shanghaiense
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3608 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A316JD95|A0A316JD95_9RHIZ Peptidase M14 OS=Falsochrobactrum shanghaiense OX=2201899 GN=DKP76_01280 PE=4 SV=1
MM1 pKa = 7.47 KK2 pKa = 10.27 KK3 pKa = 10.03 IILIATTAFFTLPCAAFAQDD23 pKa = 3.22 RR24 pKa = 11.84 VIIEE28 pKa = 4.13 VPRR31 pKa = 11.84 TVQDD35 pKa = 3.43 YY36 pKa = 10.91 VIAHH40 pKa = 6.98 PADD43 pKa = 3.62 PVIIDD48 pKa = 3.8 DD49 pKa = 5.7 DD50 pKa = 3.77 IGEE53 pKa = 4.54 GYY55 pKa = 10.13 ILPEE59 pKa = 4.38 AVTVIPIPDD68 pKa = 4.46 DD69 pKa = 3.8 PDD71 pKa = 3.29 FGYY74 pKa = 10.15 IYY76 pKa = 10.96 VDD78 pKa = 3.63 GKK80 pKa = 9.9 PVIVSMQNRR89 pKa = 11.84 RR90 pKa = 11.84 VVYY93 pKa = 10.26 YY94 pKa = 10.69 SEE96 pKa = 5.55 GPDD99 pKa = 3.71 AGPLIPDD106 pKa = 3.83 DD107 pKa = 3.6 VVTYY111 pKa = 10.61 IEE113 pKa = 4.82 TNPSPPVIYY122 pKa = 10.16 EE123 pKa = 4.05 DD124 pKa = 4.03 EE125 pKa = 4.22 LVEE128 pKa = 4.86 GAILPDD134 pKa = 5.37 DD135 pKa = 4.29 IPLEE139 pKa = 4.41 IIPDD143 pKa = 3.92 EE144 pKa = 4.0 PTYY147 pKa = 11.12 SYY149 pKa = 10.68 IYY151 pKa = 10.53 VDD153 pKa = 3.53 GRR155 pKa = 11.84 PALVEE160 pKa = 3.99 SGTRR164 pKa = 11.84 RR165 pKa = 11.84 ILWVRR170 pKa = 3.55
Molecular weight: 18.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.756
IPC2_protein 3.923
IPC_protein 3.91
Toseland 3.694
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.834
Rodwell 3.732
Grimsley 3.605
Solomon 3.897
Lehninger 3.846
Nozaki 4.012
DTASelect 4.253
Thurlkill 3.745
EMBOSS 3.846
Sillero 4.037
Patrickios 1.914
IPC_peptide 3.884
IPC2_peptide 4.012
IPC2.peptide.svr19 3.914
Protein with the highest isoelectric point:
>tr|A0A316J5V9|A0A316J5V9_9RHIZ Alcohol dehydrogenase OS=Falsochrobactrum shanghaiense OX=2201899 GN=DKP76_14635 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.09 QPSKK9 pKa = 8.8 IVRR12 pKa = 11.84 KK13 pKa = 9.24 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.55 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATTGGRR28 pKa = 11.84 KK29 pKa = 9.01 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.03 RR41 pKa = 11.84 LSAA44 pKa = 4.03
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3608
0
3608
1107300
25
3827
306.9
33.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.594 ± 0.05
0.796 ± 0.013
5.645 ± 0.034
6.058 ± 0.041
3.886 ± 0.025
8.222 ± 0.049
2.05 ± 0.02
5.985 ± 0.03
3.854 ± 0.036
9.89 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.633 ± 0.02
3.065 ± 0.024
4.771 ± 0.027
3.321 ± 0.023
6.694 ± 0.039
5.756 ± 0.026
5.116 ± 0.033
7.046 ± 0.035
1.244 ± 0.016
2.373 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here