Mycoplasma cloacale
Average proteome isoelectric point is 7.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 539 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z4LMB3|A0A2Z4LMB3_9MOLU Uncharacterized protein OS=Mycoplasma cloacale OX=92401 GN=DK849_02485 PE=4 SV=1
MM1 pKa = 7.34 TNKK4 pKa = 10.19 KK5 pKa = 10.0 YY6 pKa = 10.62 FPLTKK11 pKa = 10.37 EE12 pKa = 3.95 EE13 pKa = 4.25 LKK15 pKa = 11.08 EE16 pKa = 3.82 LVNNLDD22 pKa = 3.84 IYY24 pKa = 11.17 LGDD27 pKa = 3.69 IDD29 pKa = 4.12 TSRR32 pKa = 11.84 MFDD35 pKa = 3.23 MSSLFQNSLRR45 pKa = 11.84 KK46 pKa = 9.98 KK47 pKa = 10.15 FDD49 pKa = 4.72 GIEE52 pKa = 3.82 TWNTSNVLNMEE63 pKa = 4.2 RR64 pKa = 11.84 MFEE67 pKa = 3.99 SSFINKK73 pKa = 8.73 PLNFDD78 pKa = 3.53 TSNVKK83 pKa = 10.3 NMEE86 pKa = 4.01 YY87 pKa = 10.03 MFADD91 pKa = 4.62 CYY93 pKa = 11.22 LFNQPINFNTSKK105 pKa = 10.34 VKK107 pKa = 10.56 NMKK110 pKa = 10.5 NMFSNCYY117 pKa = 9.87 FFNQNLNWDD126 pKa = 3.83 TSNVKK131 pKa = 10.19 NMSYY135 pKa = 9.54 MFEE138 pKa = 4.1 NCHH141 pKa = 6.75 SFNQPLNWDD150 pKa = 3.67 TSNVEE155 pKa = 3.63 NMSYY159 pKa = 9.64 MFRR162 pKa = 11.84 GCHH165 pKa = 6.06 SFNQPLDD172 pKa = 3.39 WDD174 pKa = 4.25 TTNLTDD180 pKa = 3.69 TYY182 pKa = 11.5 SMFSGCRR189 pKa = 11.84 SFNQPLNWDD198 pKa = 3.79 TTNVEE203 pKa = 3.98 DD204 pKa = 3.57 MSYY207 pKa = 9.58 MFEE210 pKa = 4.21 YY211 pKa = 10.36 CHH213 pKa = 7.11 SFNQPLNWDD222 pKa = 3.67 TSNVEE227 pKa = 3.88 NMSLMFYY234 pKa = 9.93 YY235 pKa = 10.28 CHH237 pKa = 7.29 SFNQPLNWDD246 pKa = 3.72 TSNVEE251 pKa = 4.44 DD252 pKa = 3.71 MSKK255 pKa = 9.4 MFEE258 pKa = 3.94 YY259 pKa = 10.26 CYY261 pKa = 10.96 SFNQPLDD268 pKa = 3.35 WDD270 pKa = 3.99 TSNVEE275 pKa = 4.28 DD276 pKa = 4.1 MSDD279 pKa = 3.06 MFEE282 pKa = 4.19 NCHH285 pKa = 6.73 SFNQPLNWDD294 pKa = 3.67 TSNVEE299 pKa = 3.77 NMAGMFKK306 pKa = 10.37 NCHH309 pKa = 6.03 SFNQPLDD316 pKa = 3.46 WDD318 pKa = 3.93 TSNVKK323 pKa = 10.42 DD324 pKa = 3.81 MNFMFCEE331 pKa = 4.56 CSAFNQPLDD340 pKa = 3.39 WDD342 pKa = 4.08 TTNVEE347 pKa = 3.8 NMEE350 pKa = 4.1 YY351 pKa = 10.05 MFWNCKK357 pKa = 6.9 EE358 pKa = 4.56 LEE360 pKa = 4.06 YY361 pKa = 11.18 EE362 pKa = 4.55 MNFKK366 pKa = 9.95 MPYY369 pKa = 10.12 NKK371 pKa = 9.03 TKK373 pKa = 11.09 NMFHH377 pKa = 6.71 GCILLSNEE385 pKa = 4.16 EE386 pKa = 4.1 EE387 pKa = 4.29 DD388 pKa = 5.34 NEE390 pKa = 4.56 TKK392 pKa = 10.42 HH393 pKa = 6.42 SKK395 pKa = 9.2 EE396 pKa = 3.61 WFNDD400 pKa = 3.02 QKK402 pKa = 11.17 EE403 pKa = 4.1 IDD405 pKa = 4.88 EE406 pKa = 4.48 IPLLKK411 pKa = 10.47 FKK413 pKa = 10.58 IIEE416 pKa = 4.33 NDD418 pKa = 3.83 DD419 pKa = 3.71 DD420 pKa = 5.12 DD421 pKa = 4.88 EE422 pKa = 4.35 NN423 pKa = 4.41
Molecular weight: 50.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.98
IPC2_protein 4.266
IPC_protein 4.24
Toseland 4.062
ProMoST 4.368
Dawson 4.202
Bjellqvist 4.355
Wikipedia 4.088
Rodwell 4.075
Grimsley 3.961
Solomon 4.202
Lehninger 4.151
Nozaki 4.304
DTASelect 4.507
Thurlkill 4.075
EMBOSS 4.113
Sillero 4.355
Patrickios 1.519
IPC_peptide 4.202
IPC2_peptide 4.342
IPC2.peptide.svr19 4.238
Protein with the highest isoelectric point:
>tr|A0A2Z4LLZ1|A0A2Z4LLZ1_9MOLU 30S ribosomal protein S5 OS=Mycoplasma cloacale OX=92401 GN=rpsE PE=3 SV=1
MM1 pKa = 7.33 ARR3 pKa = 11.84 KK4 pKa = 9.63 ALMVKK9 pKa = 10.38 AEE11 pKa = 4.31 RR12 pKa = 11.84 EE13 pKa = 4.05 PKK15 pKa = 10.35 FGVRR19 pKa = 11.84 KK20 pKa = 7.54 YY21 pKa = 8.6 TRR23 pKa = 11.84 CQICGRR29 pKa = 11.84 VHH31 pKa = 6.76 AVLRR35 pKa = 11.84 KK36 pKa = 9.58 YY37 pKa = 10.23 KK38 pKa = 9.85 ICRR41 pKa = 11.84 ICFRR45 pKa = 11.84 EE46 pKa = 3.88 LAHH49 pKa = 6.54 EE50 pKa = 4.41 GKK52 pKa = 10.21 IPGVKK57 pKa = 9.38 KK58 pKa = 10.89 ASWW61 pKa = 3.03
Molecular weight: 7.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.304
IPC2_protein 9.633
IPC_protein 9.897
Toseland 10.701
ProMoST 10.233
Dawson 10.774
Bjellqvist 10.409
Wikipedia 10.906
Rodwell 11.257
Grimsley 10.804
Solomon 10.847
Lehninger 10.833
Nozaki 10.701
DTASelect 10.394
Thurlkill 10.687
EMBOSS 11.082
Sillero 10.701
Patrickios 11.008
IPC_peptide 10.847
IPC2_peptide 9.472
IPC2.peptide.svr19 8.533
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
539
0
539
201187
37
2772
373.3
42.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.257 ± 0.116
0.596 ± 0.03
5.439 ± 0.082
7.068 ± 0.106
5.055 ± 0.107
4.324 ± 0.105
1.614 ± 0.037
10.169 ± 0.124
9.773 ± 0.092
9.598 ± 0.092
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.882 ± 0.051
8.237 ± 0.166
2.656 ± 0.058
3.232 ± 0.049
2.889 ± 0.059
6.26 ± 0.075
5.301 ± 0.072
5.244 ± 0.071
0.941 ± 0.036
4.463 ± 0.072
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here