Paraeggerthella hongkongensis
Average proteome isoelectric point is 5.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2391 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A369LCR0|A0A369LCR0_9ACTN Uncharacterized protein OS=Paraeggerthella hongkongensis OX=230658 GN=C1879_08450 PE=4 SV=1
MM1 pKa = 7.48 AATVKK6 pKa = 9.88 TLLEE10 pKa = 4.14 HH11 pKa = 6.97 HH12 pKa = 6.94 GEE14 pKa = 4.4 LYY16 pKa = 10.12 WGTRR20 pKa = 11.84 QANPGYY26 pKa = 10.35 HH27 pKa = 6.35 YY28 pKa = 10.98 GLSLDD33 pKa = 3.27 IGEE36 pKa = 4.91 NGVAAQVVYY45 pKa = 11.23 VMSDD49 pKa = 2.83 LDD51 pKa = 4.13 EE52 pKa = 4.76 NDD54 pKa = 3.74 NPVVTPEE61 pKa = 4.33 MLMSCYY67 pKa = 9.42 TVADD71 pKa = 4.43 LEE73 pKa = 4.68 ANGIVPSDD81 pKa = 4.33 LMDD84 pKa = 4.17 EE85 pKa = 4.36 EE86 pKa = 6.48 DD87 pKa = 3.65 NDD89 pKa = 4.58 SVKK92 pKa = 10.37 GWYY95 pKa = 9.95 CIEE98 pKa = 3.85 EE99 pKa = 4.3 SFFPEE104 pKa = 4.01 NQVEE108 pKa = 4.63 ALQEE112 pKa = 4.29 SNAQLDD118 pKa = 4.37 GYY120 pKa = 10.84 LDD122 pKa = 3.43 IVLDD126 pKa = 3.59 IMLISPEE133 pKa = 3.88 EE134 pKa = 4.05 VAAGMPSLDD143 pKa = 4.78 DD144 pKa = 3.5 ISFFDD149 pKa = 5.06 LLSEE153 pKa = 4.31 LEE155 pKa = 4.84 GIAEE159 pKa = 4.78 RR160 pKa = 11.84 IDD162 pKa = 3.43 SGALAKK168 pKa = 9.83 PLPFGFRR175 pKa = 11.84 LVGDD179 pKa = 4.34 ALFGWEE185 pKa = 4.64 FADD188 pKa = 4.53 EE189 pKa = 4.2 EE190 pKa = 4.4 DD191 pKa = 3.51 WRR193 pKa = 5.44
Molecular weight: 21.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.729
IPC2_protein 3.656
IPC_protein 3.643
Toseland 3.439
ProMoST 3.795
Dawson 3.63
Bjellqvist 3.783
Wikipedia 3.541
Rodwell 3.478
Grimsley 3.35
Solomon 3.617
Lehninger 3.567
Nozaki 3.745
DTASelect 3.935
Thurlkill 3.49
EMBOSS 3.554
Sillero 3.757
Patrickios 1.024
IPC_peptide 3.617
IPC2_peptide 3.745
IPC2.peptide.svr19 3.715
Protein with the highest isoelectric point:
>tr|A0A369L4U7|A0A369L4U7_9ACTN FHA domain-containing protein OS=Paraeggerthella hongkongensis OX=230658 GN=C1879_11905 PE=4 SV=1
MM1 pKa = 7.62 RR2 pKa = 11.84 AARR5 pKa = 11.84 CASRR9 pKa = 11.84 RR10 pKa = 11.84 NARR13 pKa = 11.84 RR14 pKa = 11.84 ANALLLALGILLIASALASLMLGRR38 pKa = 11.84 YY39 pKa = 8.03 PITPVEE45 pKa = 4.08 AVGMLANLAFPVDD58 pKa = 4.35 PFWTPQQEE66 pKa = 4.42 TLFFQVRR73 pKa = 11.84 LPRR76 pKa = 11.84 IVLAVMVGCSLATAGAAYY94 pKa = 10.13 QGTFQNPLVSPDD106 pKa = 3.37 ILGASQGAAFGAACAILLGMGAFGVSASAFLFSIAAVMLVLLIGTRR152 pKa = 11.84 ARR154 pKa = 11.84 GNHH157 pKa = 5.8 LLIVVLAGVMVSSLFSAGVSFTKK180 pKa = 10.9 LIADD184 pKa = 4.55 PNNQLAAITYY194 pKa = 8.41 WLMGSLTGAKK204 pKa = 9.05 WSDD207 pKa = 3.22 MALAAIPMAVGLGALFALRR226 pKa = 11.84 WRR228 pKa = 11.84 INVLTMGDD236 pKa = 4.25 DD237 pKa = 3.74 EE238 pKa = 5.72 ASTMGVNARR247 pKa = 11.84 RR248 pKa = 11.84 TRR250 pKa = 11.84 LIVILAATLITASSVAVSGMIGWVGLVIPHH280 pKa = 7.26 LSRR283 pKa = 11.84 MVIGCDD289 pKa = 3.48 YY290 pKa = 11.05 RR291 pKa = 11.84 KK292 pKa = 10.11 LLPASMLMGASFMLIVDD309 pKa = 4.85 DD310 pKa = 4.32 IARR313 pKa = 11.84 LVATAEE319 pKa = 3.94 IPIGILTAFVGAPFFLYY336 pKa = 10.89 LITRR340 pKa = 11.84 KK341 pKa = 9.39 KK342 pKa = 10.44 QKK344 pKa = 10.45 LL345 pKa = 3.45
Molecular weight: 36.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.347
IPC2_protein 9.604
IPC_protein 10.467
Toseland 10.467
ProMoST 10.233
Dawson 10.613
Bjellqvist 10.365
Wikipedia 10.847
Rodwell 10.745
Grimsley 10.687
Solomon 10.716
Lehninger 10.672
Nozaki 10.496
DTASelect 10.35
Thurlkill 10.496
EMBOSS 10.877
Sillero 10.555
Patrickios 10.438
IPC_peptide 10.716
IPC2_peptide 9.589
IPC2.peptide.svr19 8.502
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2391
0
2391
817088
37
2536
341.7
37.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.976 ± 0.07
1.593 ± 0.023
6.083 ± 0.039
6.303 ± 0.047
3.923 ± 0.035
8.18 ± 0.052
1.73 ± 0.018
4.811 ± 0.048
3.806 ± 0.041
9.381 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.671 ± 0.026
2.84 ± 0.028
4.309 ± 0.029
2.955 ± 0.026
6.02 ± 0.056
6.096 ± 0.045
5.061 ± 0.032
8.355 ± 0.047
1.08 ± 0.021
2.827 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here