Amino acid dipepetide frequency for Lachnospiraceae bacterium OF09-33XD

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.263AlaAla: 9.263 ± 0.164	1.375AlaCys: 1.375 ± 0.04	4.753AlaAsp: 4.753 ± 0.081	6.193AlaGlu: 6.193 ± 0.095	3.181AlaPhe: 3.181 ± 0.075	7.686AlaGly: 7.686 ± 0.109	1.164AlaHis: 1.164 ± 0.036	4.659AlaIle: 4.659 ± 0.075	3.923AlaLys: 3.923 ± 0.067	7.394AlaLeu: 7.394 ± 0.099	2.432AlaMet: 2.432 ± 0.06	2.305AlaAsn: 2.305 ± 0.055	2.313AlaPro: 2.313 ± 0.048	2.275AlaGln: 2.275 ± 0.054	3.611AlaArg: 3.611 ± 0.065	4.522AlaSer: 4.522 ± 0.078	2.986AlaThr: 2.986 ± 0.063	7.298AlaVal: 7.298 ± 0.098	0.752AlaTrp: 0.752 ± 0.029	2.773AlaTyr: 2.773 ± 0.051	0.0AlaXaa: 0.0 ± 0.0
Cys	1.213CysAla: 1.213 ± 0.035	0.367CysCys: 0.367 ± 0.021	0.814CysAsp: 0.814 ± 0.025	0.995CysGlu: 0.995 ± 0.031	0.697CysPhe: 0.697 ± 0.025	1.963CysGly: 1.963 ± 0.053	0.327CysHis: 0.327 ± 0.018	1.113CysIle: 1.113 ± 0.04	0.659CysLys: 0.659 ± 0.027	1.404CysLeu: 1.404 ± 0.038	0.508CysMet: 0.508 ± 0.023	0.524CysAsn: 0.524 ± 0.021	0.712CysPro: 0.712 ± 0.03	0.499CysGln: 0.499 ± 0.025	1.042CysArg: 1.042 ± 0.033	1.043CysSer: 1.043 ± 0.031	0.813CysThr: 0.813 ± 0.033	1.029CysVal: 1.029 ± 0.035	0.157CysTrp: 0.157 ± 0.014	0.638CysTyr: 0.638 ± 0.026	0.0CysXaa: 0.0 ± 0.0
Asp	4.201AspAla: 4.201 ± 0.08	0.914AspCys: 0.914 ± 0.032	2.45AspAsp: 2.45 ± 0.063	4.109AspGlu: 4.109 ± 0.073	2.376AspPhe: 2.376 ± 0.046	4.652AspGly: 4.652 ± 0.087	0.94AspHis: 0.94 ± 0.034	3.883AspIle: 3.883 ± 0.064	2.672AspLys: 2.672 ± 0.047	4.621AspLeu: 4.621 ± 0.069	1.775AspMet: 1.775 ± 0.051	1.815AspAsn: 1.815 ± 0.049	1.988AspPro: 1.988 ± 0.053	1.684AspGln: 1.684 ± 0.04	2.792AspArg: 2.792 ± 0.06	3.032AspSer: 3.032 ± 0.063	2.978AspThr: 2.978 ± 0.068	3.565AspVal: 3.565 ± 0.058	0.667AspTrp: 0.667 ± 0.025	2.637AspTyr: 2.637 ± 0.054	0.0AspXaa: 0.0 ± 0.0
Glu	6.339GluAla: 6.339 ± 0.089	0.861GluCys: 0.861 ± 0.032	4.176GluAsp: 4.176 ± 0.071	7.533GluGlu: 7.533 ± 0.101	2.474GluPhe: 2.474 ± 0.057	4.903GluGly: 4.903 ± 0.073	1.357GluHis: 1.357 ± 0.038	5.602GluIle: 5.602 ± 0.093	5.805GluLys: 5.805 ± 0.089	7.162GluLeu: 7.162 ± 0.097	2.317GluMet: 2.317 ± 0.052	3.677GluAsn: 3.677 ± 0.063	2.082GluPro: 2.082 ± 0.047	3.12GluGln: 3.12 ± 0.068	3.744GluArg: 3.744 ± 0.081	3.394GluSer: 3.394 ± 0.058	3.788GluThr: 3.788 ± 0.072	4.501GluVal: 4.501 ± 0.066	0.728GluTrp: 0.728 ± 0.025	3.059GluTyr: 3.059 ± 0.061	0.0GluXaa: 0.0 ± 0.0
Phe	2.771PheAla: 2.771 ± 0.058	0.757PheCys: 0.757 ± 0.027	2.209PheAsp: 2.209 ± 0.044	2.475PheGlu: 2.475 ± 0.059	1.606PhePhe: 1.606 ± 0.049	3.048PheGly: 3.048 ± 0.058	0.873PheHis: 0.873 ± 0.029	2.416PheIle: 2.416 ± 0.058	1.497PheLys: 1.497 ± 0.04	3.895PheLeu: 3.895 ± 0.08	1.036PheMet: 1.036 ± 0.033	1.306PheAsn: 1.306 ± 0.04	1.469PhePro: 1.469 ± 0.036	1.478PheGln: 1.478 ± 0.038	2.06PheArg: 2.06 ± 0.047	2.895PheSer: 2.895 ± 0.052	2.218PheThr: 2.218 ± 0.053	2.367PheVal: 2.367 ± 0.054	0.404PheTrp: 0.404 ± 0.022	1.637PheTyr: 1.637 ± 0.04	0.0PheXaa: 0.0 ± 0.0
Gly	5.923GlyAla: 5.923 ± 0.097	1.569GlyCys: 1.569 ± 0.049	3.654GlyAsp: 3.654 ± 0.064	5.206GlyGlu: 5.206 ± 0.077	3.122GlyPhe: 3.122 ± 0.057	6.012GlyGly: 6.012 ± 0.093	1.322GlyHis: 1.322 ± 0.039	6.41GlyIle: 6.41 ± 0.1	5.146GlyLys: 5.146 ± 0.081	6.382GlyLeu: 6.382 ± 0.081	2.612GlyMet: 2.612 ± 0.048	3.185GlyAsn: 3.185 ± 0.062	1.551GlyPro: 1.551 ± 0.047	2.543GlyGln: 2.543 ± 0.053	4.095GlyArg: 4.095 ± 0.082	4.799GlySer: 4.799 ± 0.085	4.678GlyThr: 4.678 ± 0.089	5.305GlyVal: 5.305 ± 0.077	0.809GlyTrp: 0.809 ± 0.032	3.242GlyTyr: 3.242 ± 0.064	0.0GlyXaa: 0.0 ± 0.0
His	1.151HisAla: 1.151 ± 0.033	0.31HisCys: 0.31 ± 0.019	0.84HisAsp: 0.84 ± 0.035	1.087HisGlu: 1.087 ± 0.034	0.786HisPhe: 0.786 ± 0.03	1.331HisGly: 1.331 ± 0.042	0.408HisHis: 0.408 ± 0.032	1.239HisIle: 1.239 ± 0.043	0.837HisLys: 0.837 ± 0.027	1.541HisLeu: 1.541 ± 0.044	0.582HisMet: 0.582 ± 0.028	0.632HisAsn: 0.632 ± 0.024	0.932HisPro: 0.932 ± 0.037	0.573HisGln: 0.573 ± 0.024	0.952HisArg: 0.952 ± 0.033	1.021HisSer: 1.021 ± 0.036	1.021HisThr: 1.021 ± 0.036	1.13HisVal: 1.13 ± 0.035	0.189HisTrp: 0.189 ± 0.014	0.725HisTyr: 0.725 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	5.322IleAla: 5.322 ± 0.076	1.499IleCys: 1.499 ± 0.038	3.535IleAsp: 3.535 ± 0.065	4.085IleGlu: 4.085 ± 0.069	2.69IlePhe: 2.69 ± 0.054	4.951IleGly: 4.951 ± 0.08	1.361IleHis: 1.361 ± 0.038	4.222IleIle: 4.222 ± 0.086	3.086IleLys: 3.086 ± 0.059	7.058IleLeu: 7.058 ± 0.103	1.776IleMet: 1.776 ± 0.041	2.385IleAsn: 2.385 ± 0.052	3.158IlePro: 3.158 ± 0.057	2.362IleGln: 2.362 ± 0.046	3.983IleArg: 3.983 ± 0.062	4.703IleSer: 4.703 ± 0.072	3.922IleThr: 3.922 ± 0.063	4.247IleVal: 4.247 ± 0.068	0.658IleTrp: 0.658 ± 0.03	2.625IleTyr: 2.625 ± 0.057	0.0IleXaa: 0.0 ± 0.0
Lys	4.666LysAla: 4.666 ± 0.078	0.661LysCys: 0.661 ± 0.027	3.078LysAsp: 3.078 ± 0.053	5.275LysGlu: 5.275 ± 0.087	1.528LysPhe: 1.528 ± 0.038	3.97LysGly: 3.97 ± 0.069	0.899LysHis: 0.899 ± 0.033	4.053LysIle: 4.053 ± 0.069	4.866LysLys: 4.866 ± 0.075	4.743LysLeu: 4.743 ± 0.087	1.873LysMet: 1.873 ± 0.048	2.754LysAsn: 2.754 ± 0.055	1.882LysPro: 1.882 ± 0.049	1.975LysGln: 1.975 ± 0.047	3.069LysArg: 3.069 ± 0.054	2.993LysSer: 2.993 ± 0.053	3.105LysThr: 3.105 ± 0.062	3.566LysVal: 3.566 ± 0.064	0.569LysTrp: 0.569 ± 0.023	2.427LysTyr: 2.427 ± 0.052	0.0LysXaa: 0.0 ± 0.0
Leu	7.53LeuAla: 7.53 ± 0.109	1.581LeuCys: 1.581 ± 0.038	5.07LeuAsp: 5.07 ± 0.073	7.054LeuGlu: 7.054 ± 0.102	3.577LeuPhe: 3.577 ± 0.075	6.095LeuGly: 6.095 ± 0.086	1.557LeuHis: 1.557 ± 0.041	5.816LeuIle: 5.816 ± 0.085	5.774LeuLys: 5.774 ± 0.084	9.189LeuLeu: 9.189 ± 0.139	2.826LeuMet: 2.826 ± 0.053	3.745LeuAsn: 3.745 ± 0.065	3.919LeuPro: 3.919 ± 0.063	3.187LeuGln: 3.187 ± 0.061	4.352LeuArg: 4.352 ± 0.087	6.328LeuSer: 6.328 ± 0.097	5.115LeuThr: 5.115 ± 0.073	5.588LeuVal: 5.588 ± 0.088	0.747LeuTrp: 0.747 ± 0.032	3.314LeuTyr: 3.314 ± 0.073	0.0LeuXaa: 0.0 ± 0.0
Met	2.581MetAla: 2.581 ± 0.057	0.383MetCys: 0.383 ± 0.02	1.913MetAsp: 1.913 ± 0.041	2.587MetGlu: 2.587 ± 0.055	0.965MetPhe: 0.965 ± 0.032	2.195MetGly: 2.195 ± 0.045	0.458MetHis: 0.458 ± 0.023	2.166MetIle: 2.166 ± 0.048	2.438MetLys: 2.438 ± 0.042	2.548MetLeu: 2.548 ± 0.055	0.916MetMet: 0.916 ± 0.036	1.494MetAsn: 1.494 ± 0.037	1.16MetPro: 1.16 ± 0.037	0.976MetGln: 0.976 ± 0.031	1.378MetArg: 1.378 ± 0.039	1.778MetSer: 1.778 ± 0.039	1.726MetThr: 1.726 ± 0.044	1.974MetVal: 1.974 ± 0.047	0.207MetTrp: 0.207 ± 0.014	0.874MetTyr: 0.874 ± 0.029	0.0MetXaa: 0.0 ± 0.0
Asn	2.776AsnAla: 2.776 ± 0.057	0.689AsnCys: 0.689 ± 0.028	1.827AsnAsp: 1.827 ± 0.042	2.361AsnGlu: 2.361 ± 0.047	1.393AsnPhe: 1.393 ± 0.037	3.379AsnGly: 3.379 ± 0.062	0.772AsnHis: 0.772 ± 0.028	2.814AsnIle: 2.814 ± 0.057	1.822AsnLys: 1.822 ± 0.047	3.63AsnLeu: 3.63 ± 0.051	1.231AsnMet: 1.231 ± 0.032	1.427AsnAsn: 1.427 ± 0.046	1.982AsnPro: 1.982 ± 0.043	1.519AsnGln: 1.519 ± 0.042	2.088AsnArg: 2.088 ± 0.051	2.337AsnSer: 2.337 ± 0.049	2.101AsnThr: 2.101 ± 0.045	2.538AsnVal: 2.538 ± 0.057	0.381AsnTrp: 0.381 ± 0.021	1.712AsnTyr: 1.712 ± 0.048	0.0AsnXaa: 0.0 ± 0.0
Pro	3.043ProAla: 3.043 ± 0.056	0.538ProCys: 0.538 ± 0.023	2.637ProAsp: 2.637 ± 0.046	3.93ProGlu: 3.93 ± 0.074	1.48ProPhe: 1.48 ± 0.033	3.02ProGly: 3.02 ± 0.06	0.585ProHis: 0.585 ± 0.025	1.752ProIle: 1.752 ± 0.045	1.589ProLys: 1.589 ± 0.044	2.899ProLeu: 2.899 ± 0.061	1.004ProMet: 1.004 ± 0.033	1.109ProAsn: 1.109 ± 0.038	0.958ProPro: 0.958 ± 0.031	1.066ProGln: 1.066 ± 0.033	1.234ProArg: 1.234 ± 0.037	2.043ProSer: 2.043 ± 0.049	1.592ProThr: 1.592 ± 0.047	3.424ProVal: 3.424 ± 0.056	0.343ProTrp: 0.343 ± 0.017	1.473ProTyr: 1.473 ± 0.042	0.0ProXaa: 0.0 ± 0.0
Gln	2.933GlnAla: 2.933 ± 0.066	0.434GlnCys: 0.434 ± 0.019	1.585GlnAsp: 1.585 ± 0.043	3.056GlnGlu: 3.056 ± 0.059	1.197GlnPhe: 1.197 ± 0.031	2.212GlnGly: 2.212 ± 0.04	0.443GlnHis: 0.443 ± 0.019	2.59GlnIle: 2.59 ± 0.058	2.583GlnLys: 2.583 ± 0.058	2.96GlnLeu: 2.96 ± 0.052	1.305GlnMet: 1.305 ± 0.034	1.626GlnAsn: 1.626 ± 0.044	1.142GlnPro: 1.142 ± 0.038	1.376GlnGln: 1.376 ± 0.045	1.547GlnArg: 1.547 ± 0.041	1.76GlnSer: 1.76 ± 0.046	1.651GlnThr: 1.651 ± 0.044	2.395GlnVal: 2.395 ± 0.044	0.326GlnTrp: 0.326 ± 0.017	1.383GlnTyr: 1.383 ± 0.041	0.0GlnXaa: 0.0 ± 0.0
Arg	3.304ArgAla: 3.304 ± 0.06	0.706ArgCys: 0.706 ± 0.025	2.487ArgAsp: 2.487 ± 0.047	4.679ArgGlu: 4.679 ± 0.083	1.897ArgPhe: 1.897 ± 0.052	3.045ArgGly: 3.045 ± 0.058	0.881ArgHis: 0.881 ± 0.031	3.818ArgIle: 3.818 ± 0.064	3.764ArgLys: 3.764 ± 0.071	4.824ArgLeu: 4.824 ± 0.072	1.746ArgMet: 1.746 ± 0.047	2.158ArgAsn: 2.158 ± 0.05	1.667ArgPro: 1.667 ± 0.048	2.107ArgGln: 2.107 ± 0.053	3.197ArgArg: 3.197 ± 0.076	2.526ArgSer: 2.526 ± 0.051	2.52ArgThr: 2.52 ± 0.051	3.044ArgVal: 3.044 ± 0.056	0.487ArgTrp: 0.487 ± 0.022	1.92ArgTyr: 1.92 ± 0.051	0.0ArgXaa: 0.0 ± 0.0
Ser	4.827SerAla: 4.827 ± 0.084	1.06SerCys: 1.06 ± 0.037	3.242SerAsp: 3.242 ± 0.064	3.938SerGlu: 3.938 ± 0.056	2.576SerPhe: 2.576 ± 0.055	5.926SerGly: 5.926 ± 0.093	1.028SerHis: 1.028 ± 0.033	3.8SerIle: 3.8 ± 0.069	2.601SerLys: 2.601 ± 0.057	5.576SerLeu: 5.576 ± 0.088	1.862SerMet: 1.862 ± 0.045	2.036SerAsn: 2.036 ± 0.046	1.967SerPro: 1.967 ± 0.043	1.956SerGln: 1.956 ± 0.046	3.223SerArg: 3.223 ± 0.067	4.066SerSer: 4.066 ± 0.094	2.59SerThr: 2.59 ± 0.053	4.451SerVal: 4.451 ± 0.076	0.675SerTrp: 0.675 ± 0.028	2.42SerTyr: 2.42 ± 0.06	0.0SerXaa: 0.0 ± 0.0
Thr	5.002ThrAla: 5.002 ± 0.09	0.69ThrCys: 0.69 ± 0.028	3.05ThrAsp: 3.05 ± 0.075	3.938ThrGlu: 3.938 ± 0.072	1.955ThrPhe: 1.955 ± 0.052	5.019ThrGly: 5.019 ± 0.088	0.803ThrHis: 0.803 ± 0.027	3.259ThrIle: 3.259 ± 0.057	2.406ThrLys: 2.406 ± 0.063	4.731ThrLeu: 4.731 ± 0.077	1.374ThrMet: 1.374 ± 0.035	1.764ThrAsn: 1.764 ± 0.053	2.33ThrPro: 2.33 ± 0.059	1.457ThrGln: 1.457 ± 0.046	2.079ThrArg: 2.079 ± 0.053	2.934ThrSer: 2.934 ± 0.064	2.479ThrThr: 2.479 ± 0.067	4.741ThrVal: 4.741 ± 0.089	0.489ThrTrp: 0.489 ± 0.024	1.977ThrTyr: 1.977 ± 0.051	0.0ThrXaa: 0.0 ± 0.0
Val	4.807ValAla: 4.807 ± 0.077	1.285ValCys: 1.285 ± 0.036	3.547ValAsp: 3.547 ± 0.063	4.46ValGlu: 4.46 ± 0.07	2.875ValPhe: 2.875 ± 0.053	4.258ValGly: 4.258 ± 0.064	1.151ValHis: 1.151 ± 0.037	5.012ValIle: 5.012 ± 0.073	3.99ValLys: 3.99 ± 0.065	6.968ValLeu: 6.968 ± 0.097	2.119ValMet: 2.119 ± 0.049	2.789ValAsn: 2.789 ± 0.053	2.772ValPro: 2.772 ± 0.055	2.12ValGln: 2.12 ± 0.05	3.499ValArg: 3.499 ± 0.062	4.756ValSer: 4.756 ± 0.074	4.479ValThr: 4.479 ± 0.094	4.548ValVal: 4.548 ± 0.075	0.657ValTrp: 0.657 ± 0.028	2.654ValTyr: 2.654 ± 0.054	0.0ValXaa: 0.0 ± 0.0
Trp	0.6TrpAla: 0.6 ± 0.026	0.157TrpCys: 0.157 ± 0.012	0.555TrpAsp: 0.555 ± 0.024	0.778TrpGlu: 0.778 ± 0.028	0.389TrpPhe: 0.389 ± 0.018	0.711TrpGly: 0.711 ± 0.028	0.144TrpHis: 0.144 ± 0.013	0.653TrpIle: 0.653 ± 0.027	0.778TrpLys: 0.778 ± 0.026	0.929TrpLeu: 0.929 ± 0.034	0.377TrpMet: 0.377 ± 0.02	0.514TrpAsn: 0.514 ± 0.022	0.266TrpPro: 0.266 ± 0.014	0.407TrpGln: 0.407 ± 0.02	0.471TrpArg: 0.471 ± 0.025	0.53TrpSer: 0.53 ± 0.025	0.464TrpThr: 0.464 ± 0.022	0.524TrpVal: 0.524 ± 0.021	0.108TrpTrp: 0.108 ± 0.01	0.395TrpTyr: 0.395 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.604TyrAla: 2.604 ± 0.053	0.682TyrCys: 0.682 ± 0.027	2.327TyrAsp: 2.327 ± 0.05	2.883TyrGlu: 2.883 ± 0.05	1.653TyrPhe: 1.653 ± 0.046	3.191TyrGly: 3.191 ± 0.057	0.812TyrHis: 0.812 ± 0.03	2.352TyrIle: 2.352 ± 0.051	1.714TyrLys: 1.714 ± 0.05	3.91TyrLeu: 3.91 ± 0.077	1.046TyrMet: 1.046 ± 0.036	1.534TyrAsn: 1.534 ± 0.043	1.468TyrPro: 1.468 ± 0.04	1.825TyrGln: 1.825 ± 0.049	2.362TyrArg: 2.362 ± 0.056	2.362TyrSer: 2.362 ± 0.05	2.251TyrThr: 2.251 ± 0.061	2.51TyrVal: 2.51 ± 0.057	0.399TyrTrp: 0.399 ± 0.023	1.862TyrTyr: 1.862 ± 0.049	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3402 proteins (997160 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---