Human mastadenovirus C
Average proteome isoelectric point is 6.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 27 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3Q9HL43|A0A3Q9HL43_9ADEN 33K OS=Human mastadenovirus C OX=129951 PE=3 SV=1
MM1 pKa = 7.5 RR2 pKa = 11.84 HH3 pKa = 6.51 IICHH7 pKa = 5.93 GGVITEE13 pKa = 4.22 EE14 pKa = 4.15 MAASLLDD21 pKa = 3.6 QLIEE25 pKa = 4.19 EE26 pKa = 4.48 VLADD30 pKa = 3.85 NLPPPSHH37 pKa = 7.16 FEE39 pKa = 3.8 PPTLHH44 pKa = 6.78 EE45 pKa = 5.5 LYY47 pKa = 10.58 DD48 pKa = 4.45 LDD50 pKa = 3.62 VTAPEE55 pKa = 4.89 DD56 pKa = 3.83 PNEE59 pKa = 4.13 EE60 pKa = 3.85 AVSQIFPEE68 pKa = 4.34 SVMLAVQEE76 pKa = 4.97 GIDD79 pKa = 4.38 LFTFPPAPGSPEE91 pKa = 3.95 PPHH94 pKa = 7.1 LSRR97 pKa = 11.84 QPEE100 pKa = 4.1 QPEE103 pKa = 3.86 QRR105 pKa = 11.84 ALGPVSMPNLVPEE118 pKa = 4.78 VIDD121 pKa = 3.89 LTCHH125 pKa = 5.71 EE126 pKa = 5.16 AGFPPSDD133 pKa = 4.22 DD134 pKa = 3.37 EE135 pKa = 5.17 DD136 pKa = 4.38 EE137 pKa = 4.52 EE138 pKa = 5.43 GEE140 pKa = 4.43 EE141 pKa = 4.08 FVLDD145 pKa = 4.04 YY146 pKa = 11.29 VEE148 pKa = 5.48 HH149 pKa = 7.23 PGHH152 pKa = 6.6 GCRR155 pKa = 11.84 SCHH158 pKa = 4.33 YY159 pKa = 9.82 HH160 pKa = 6.49 RR161 pKa = 11.84 RR162 pKa = 11.84 NTGDD166 pKa = 3.82 PDD168 pKa = 3.74 IMCSLCYY175 pKa = 9.84 MRR177 pKa = 11.84 TCGMFVYY184 pKa = 10.41 SPVSEE189 pKa = 4.7 PEE191 pKa = 4.08 PEE193 pKa = 4.37 PEE195 pKa = 4.57 PEE197 pKa = 4.28 PEE199 pKa = 3.77 PARR202 pKa = 11.84 PARR205 pKa = 11.84 RR206 pKa = 11.84 PKK208 pKa = 9.45 MVPAILRR215 pKa = 11.84 RR216 pKa = 11.84 PTSPVSRR223 pKa = 11.84 EE224 pKa = 4.32 CNSSTDD230 pKa = 3.44 SCDD233 pKa = 3.3 SGPSNTPPEE242 pKa = 4.07 IHH244 pKa = 6.71 PVVPLCPIKK253 pKa = 10.4 PVAVRR258 pKa = 11.84 VGGRR262 pKa = 11.84 RR263 pKa = 11.84 RR264 pKa = 11.84 AVEE267 pKa = 4.43 CIEE270 pKa = 5.02 DD271 pKa = 4.03 LLNEE275 pKa = 4.64 PGQPLDD281 pKa = 4.25 LSCKK285 pKa = 10.03 RR286 pKa = 11.84 PRR288 pKa = 11.84 PP289 pKa = 3.69
Molecular weight: 31.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.479
IPC2_protein 4.571
IPC_protein 4.507
Toseland 4.38
ProMoST 4.609
Dawson 4.457
Bjellqvist 4.596
Wikipedia 4.304
Rodwell 4.368
Grimsley 4.291
Solomon 4.457
Lehninger 4.406
Nozaki 4.558
DTASelect 4.685
Thurlkill 4.38
EMBOSS 4.329
Sillero 4.635
Patrickios 3.325
IPC_peptide 4.469
IPC2_peptide 4.635
IPC2.peptide.svr19 4.542
Protein with the highest isoelectric point:
>tr|E1ARR7|E1ARR7_9ADEN Isoform of A0A3Q9HL75 Pre-hexon-linking protein VIII OS=Human mastadenovirus C OX=129951 GN=L4 PE=2 SV=1
MM1 pKa = 7.77 ALTCRR6 pKa = 11.84 LRR8 pKa = 11.84 FPVPGFRR15 pKa = 11.84 GRR17 pKa = 11.84 MHH19 pKa = 7.55 RR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 GMAGHH27 pKa = 7.2 GLTGGMRR34 pKa = 11.84 RR35 pKa = 11.84 AHH37 pKa = 5.79 HH38 pKa = 5.82 RR39 pKa = 11.84 RR40 pKa = 11.84 RR41 pKa = 11.84 RR42 pKa = 11.84 ASHH45 pKa = 5.58 RR46 pKa = 11.84 RR47 pKa = 11.84 MRR49 pKa = 11.84 GGILPLLIPLIAAAIGAVPGIASVALQAQRR79 pKa = 11.84 HH80 pKa = 4.41
Molecular weight: 8.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.096
IPC_protein 12.72
Toseland 12.881
ProMoST 13.378
Dawson 12.881
Bjellqvist 12.881
Wikipedia 13.349
Rodwell 12.369
Grimsley 12.91
Solomon 13.378
Lehninger 13.276
Nozaki 12.881
DTASelect 12.881
Thurlkill 12.881
EMBOSS 13.378
Sillero 12.881
Patrickios 12.106
IPC_peptide 13.378
IPC2_peptide 12.369
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
16
11
27
7412
61
807
274.5
30.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.338 ± 0.448
2.024 ± 0.403
4.79 ± 0.291
6.827 ± 0.473
3.319 ± 0.222
6.152 ± 0.307
2.59 ± 0.234
3.791 ± 0.281
3.386 ± 0.368
9.458 ± 0.47
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.604 ± 0.231
3.859 ± 0.378
7.366 ± 0.486
4.439 ± 0.327
8.5 ± 0.531
6.935 ± 0.372
5.491 ± 0.428
6.462 ± 0.32
1.079 ± 0.133
2.59 ± 0.226
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here