Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) (Trichophyton mentagrophytes)
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7976 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D4AWL6|D4AWL6_ARTBC L-type lectin-like domain-containing protein OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_00582 PE=4 SV=1
MM1 pKa = 7.92 DD2 pKa = 6.22 PSPSDD7 pKa = 3.47 EE8 pKa = 4.33 NPDD11 pKa = 3.39 YY12 pKa = 10.67 KK13 pKa = 10.96 QPGDD17 pKa = 3.72 HH18 pKa = 7.43 DD19 pKa = 4.2 LTSGDD24 pKa = 4.04 VEE26 pKa = 4.54 NEE28 pKa = 4.06 TLGDD32 pKa = 4.03 DD33 pKa = 3.9 YY34 pKa = 11.78 CSDD37 pKa = 4.28 FVNSQYY43 pKa = 10.64 WPVLDD48 pKa = 5.89 LPPLTNGSTRR58 pKa = 11.84 GTNEE62 pKa = 3.15 EE63 pKa = 4.72 SFPQGHH69 pKa = 5.58 QADD72 pKa = 4.99 EE73 pKa = 4.36 IYY75 pKa = 10.16 EE76 pKa = 4.0 YY77 pKa = 10.31 PPRR80 pKa = 11.84 HH81 pKa = 6.2 IEE83 pKa = 4.47 AGDD86 pKa = 3.44 TGPPGTGSNLEE97 pKa = 4.55 GVSTWQDD104 pKa = 2.12 IDD106 pKa = 2.5 IWRR109 pKa = 11.84 NLTTIPP115 pKa = 4.33
Molecular weight: 12.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.712
IPC2_protein 3.719
IPC_protein 3.694
Toseland 3.49
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.643
Rodwell 3.528
Grimsley 3.401
Solomon 3.681
Lehninger 3.643
Nozaki 3.821
DTASelect 4.05
Thurlkill 3.554
EMBOSS 3.656
Sillero 3.821
Patrickios 0.896
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.746
Protein with the highest isoelectric point:
>tr|D4AIW3|D4AIW3_ARTBC Cytochrome P450 putative OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_04211 PE=4 SV=1
MM1 pKa = 7.53 AGVAAYY7 pKa = 9.42 EE8 pKa = 3.99 VDD10 pKa = 3.49 AAAAAAVAAAAVVAVAVAVAAAAAAGRR37 pKa = 11.84 KK38 pKa = 8.59 KK39 pKa = 10.24 RR40 pKa = 11.84 RR41 pKa = 11.84 QSQRR45 pKa = 11.84 RR46 pKa = 11.84 GDD48 pKa = 3.49 PATASRR54 pKa = 11.84 RR55 pKa = 11.84 DD56 pKa = 3.59 SEE58 pKa = 4.41 PAGTASARR66 pKa = 11.84 STAEE70 pKa = 3.74 LPSWLLEE77 pKa = 3.92 LLEE80 pKa = 4.73 GQFSPSGGGSGAGNEE95 pKa = 4.69 GAGSLCFRR103 pKa = 11.84 GHH105 pKa = 6.9 APWACWLAGLLAGWLAAGSRR125 pKa = 11.84 DD126 pKa = 3.29 LDD128 pKa = 3.52 GRR130 pKa = 11.84 RR131 pKa = 11.84 KK132 pKa = 9.84 GRR134 pKa = 11.84 PGQGRR139 pKa = 11.84 TEE141 pKa = 3.91 GSEE144 pKa = 4.19 RR145 pKa = 11.84 ARR147 pKa = 11.84 QDD149 pKa = 3.11 GNGRR153 pKa = 11.84 RR154 pKa = 11.84 SRR156 pKa = 11.84 GQDD159 pKa = 2.82 GGRR162 pKa = 11.84 EE163 pKa = 3.89 GDD165 pKa = 3.54 AVEE168 pKa = 3.91 QRR170 pKa = 11.84 QRR172 pKa = 11.84 TSSRR176 pKa = 11.84 AFCFFFFFFFVSSCRR191 pKa = 11.84 FAVFFVSFAAKK202 pKa = 9.9 KK203 pKa = 8.82 PSPGGDD209 pKa = 2.83 HH210 pKa = 6.2 VTGFWSS216 pKa = 3.18
Molecular weight: 22.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 9.297
IPC_protein 10.072
Toseland 10.657
ProMoST 10.423
Dawson 10.701
Bjellqvist 10.467
Wikipedia 10.935
Rodwell 10.73
Grimsley 10.73
Solomon 10.906
Lehninger 10.877
Nozaki 10.672
DTASelect 10.438
Thurlkill 10.643
EMBOSS 11.067
Sillero 10.657
Patrickios 10.496
IPC_peptide 10.921
IPC2_peptide 9.823
IPC2.peptide.svr19 8.664
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7976
0
7976
3936409
25
7870
493.5
54.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.173 ± 0.022
1.193 ± 0.01
5.619 ± 0.02
6.395 ± 0.029
3.675 ± 0.018
6.576 ± 0.023
2.388 ± 0.013
5.118 ± 0.021
5.242 ± 0.026
8.871 ± 0.032
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.106 ± 0.01
3.816 ± 0.013
6.198 ± 0.034
4.125 ± 0.024
6.078 ± 0.021
8.781 ± 0.032
5.891 ± 0.018
5.771 ± 0.019
1.279 ± 0.008
2.705 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here