Methanomethylovorans hollandica (strain DSM 15978 / NBRC 107637 / DMS1)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia; Methanosarcinales; Methanosarcinaceae; Methanomethylovorans; Methanomethylovorans hollandica

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2551 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L0L0P4|L0L0P4_METHD Nitric oxide reductase activation protein OS=Methanomethylovorans hollandica (strain DSM 15978 / NBRC 107637 / DMS1) OX=867904 GN=Metho_1767 PE=4 SV=1
MM1 pKa = 7.17KK2 pKa = 10.54LEE4 pKa = 4.38YY5 pKa = 9.85IVGAIVILFVAQFLYY20 pKa = 11.14ALAANPGSEE29 pKa = 4.32FGGADD34 pKa = 3.35GAAEE38 pKa = 4.4DD39 pKa = 5.28LISDD43 pKa = 4.07IDD45 pKa = 3.91PDD47 pKa = 3.96YY48 pKa = 10.99EE49 pKa = 3.97AWDD52 pKa = 3.66PGFPKK57 pKa = 10.44FEE59 pKa = 4.46PPGGEE64 pKa = 4.09TEE66 pKa = 4.3SLLFALQAAIGSLVIGYY83 pKa = 7.84FFGYY87 pKa = 10.34YY88 pKa = 9.42RR89 pKa = 11.84GKK91 pKa = 9.56NQSGQQ96 pKa = 3.22

Molecular weight: 10.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L0KY20|L0KY20_METHD TIGR02687 family protein OS=Methanomethylovorans hollandica (strain DSM 15978 / NBRC 107637 / DMS1) OX=867904 GN=Metho_1384 PE=4 SV=1
MM1 pKa = 7.29NNDD4 pKa = 2.86RR5 pKa = 11.84RR6 pKa = 11.84GGSGGFRR13 pKa = 11.84SGGSGGSRR21 pKa = 11.84GGSGGSGGFRR31 pKa = 11.84SGGSGGSGGFRR42 pKa = 11.84SGGSGGFRR50 pKa = 11.84PSGPRR55 pKa = 11.84EE56 pKa = 3.73MHH58 pKa = 6.71KK59 pKa = 9.95ATCADD64 pKa = 3.91CGQEE68 pKa = 4.1TEE70 pKa = 4.59VPFVPSGDD78 pKa = 3.25RR79 pKa = 11.84PVYY82 pKa = 10.43CRR84 pKa = 11.84EE85 pKa = 4.4CYY87 pKa = 9.54QKK89 pKa = 10.34HH90 pKa = 5.31RR91 pKa = 11.84PPRR94 pKa = 11.84RR95 pKa = 11.84YY96 pKa = 9.92

Molecular weight: 9.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2551

0

2551

771326

32

2727

302.4

33.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.138 ± 0.053

1.248 ± 0.028

5.727 ± 0.038

7.027 ± 0.059

3.949 ± 0.035

6.988 ± 0.046

1.986 ± 0.021

8.214 ± 0.046

6.361 ± 0.058

9.072 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.985 ± 0.031

4.365 ± 0.044

3.815 ± 0.029

2.905 ± 0.025

4.428 ± 0.031

6.657 ± 0.046

5.554 ± 0.06

7.154 ± 0.042

0.922 ± 0.018

3.504 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski