Thalassolituus oleivorans MIL-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Oceanospirillaceae; Thalassolituus; Thalassolituus oleivorans

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3634 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M5DST1|M5DST1_9GAMM Peptidoglycan-associated protein OS=Thalassolituus oleivorans MIL-1 OX=1298593 GN=pal PE=3 SV=1
MM1 pKa = 7.66KK2 pKa = 8.89KK3 pKa = 7.91TAIAAFIVALAPVASHH19 pKa = 7.15ADD21 pKa = 3.5LLFTVGAKK29 pKa = 10.34ASVWNAEE36 pKa = 3.93PTGQIDD42 pKa = 4.34DD43 pKa = 5.43DD44 pKa = 4.45ISVDD48 pKa = 3.44SSNNGLGLDD57 pKa = 4.3SEE59 pKa = 5.2NGTQLTVFFEE69 pKa = 4.87HH70 pKa = 6.98PVPMLPNIKK79 pKa = 10.08LKK81 pKa = 8.96QTSLDD86 pKa = 3.54LSGDD90 pKa = 3.47GTLALSTSFNGQAFAGPVTSDD111 pKa = 3.82LDD113 pKa = 3.94LSHH116 pKa = 7.35TDD118 pKa = 2.99VTLYY122 pKa = 10.04WGLPLPLPYY131 pKa = 10.49VDD133 pKa = 4.84INFGLTGRR141 pKa = 11.84MFDD144 pKa = 4.12GYY146 pKa = 11.48AEE148 pKa = 4.16VSNTLSGTEE157 pKa = 4.1RR158 pKa = 11.84VDD160 pKa = 4.22LDD162 pKa = 3.35ATVPMVYY169 pKa = 10.22GAVKK173 pKa = 10.06VDD175 pKa = 3.63TPFGVYY181 pKa = 10.23AQVDD185 pKa = 3.85VNYY188 pKa = 9.91IGYY191 pKa = 9.78SGNTLSDD198 pKa = 2.81ISYY201 pKa = 10.9GLGYY205 pKa = 10.33DD206 pKa = 3.85LPVPIADD213 pKa = 3.49IGLEE217 pKa = 3.6AGYY220 pKa = 10.36RR221 pKa = 11.84SLSMKK226 pKa = 9.83TDD228 pKa = 3.31EE229 pKa = 5.34DD230 pKa = 3.81LTDD233 pKa = 3.17IATDD237 pKa = 3.34VDD239 pKa = 4.2VSGLYY244 pKa = 10.29YY245 pKa = 10.42GASVSIGFF253 pKa = 4.13

Molecular weight: 26.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M5DYQ5|M5DYQ5_9GAMM RadC gene product OS=Thalassolituus oleivorans MIL-1 OX=1298593 GN=TOL_0211 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.35NGRR28 pKa = 11.84LVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.27GRR39 pKa = 11.84HH40 pKa = 5.41SLTAA44 pKa = 3.92

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3634

0

3634

1170392

31

3596

322.1

35.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.504 ± 0.051

0.957 ± 0.013

6.023 ± 0.04

6.143 ± 0.038

3.803 ± 0.028

7.011 ± 0.047

2.202 ± 0.027

6.001 ± 0.032

4.373 ± 0.035

10.438 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.599 ± 0.023

4.036 ± 0.035

4.111 ± 0.029

4.321 ± 0.034

5.325 ± 0.041

6.471 ± 0.043

5.515 ± 0.046

6.91 ± 0.038

1.346 ± 0.017

2.911 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski