Ralstonia phage RSK1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Firingavirus; Ralstonia virus RSK1

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U6C6H0|U6C6H0_9CAUD Putative phage-related membrane protein OS=Ralstonia phage RSK1 OX=1417599 PE=4 SV=1
MM1 pKa = 7.5SLGITASVVGGIGAIAGGVISANGAQSAADD31 pKa = 3.73TQANATQSGIAQQNYY46 pKa = 7.22QFNTLRR52 pKa = 11.84GLLQPYY58 pKa = 9.72VDD60 pKa = 4.66AGTGALSGYY69 pKa = 9.96NSAVGQYY76 pKa = 10.8GSTLGQLNNLTGANGQAAQQSAISGLTSSPLYY108 pKa = 9.04ATAMDD113 pKa = 5.28LGQQSILANASATGALRR130 pKa = 11.84NGNTIASLGYY140 pKa = 10.33LPQQVLSNVMQTQIGNLGTSLSGTQSLVNQYY171 pKa = 11.28GNLLNLGEE179 pKa = 4.27NAASGTGQAAMNTGSNITNLLGQQGAAQAGGTIGTSNAINSAINGVSSAFGQYY232 pKa = 11.44ANGSNGNYY240 pKa = 9.8SFSAPAYY247 pKa = 9.14LNAGGISTNGSTTGLFNTPLFNN269 pKa = 4.8

Molecular weight: 26.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U6C720|U6C720_9CAUD Uncharacterized protein OS=Ralstonia phage RSK1 OX=1417599 PE=4 SV=1
MM1 pKa = 6.94TLEE4 pKa = 4.77HH5 pKa = 7.07IEE7 pKa = 4.4AKK9 pKa = 10.21PLADD13 pKa = 3.47RR14 pKa = 11.84FPFRR18 pKa = 11.84SHH20 pKa = 7.52RR21 pKa = 11.84ITSSGFARR29 pKa = 11.84AVVCPEE35 pKa = 2.75IWTEE39 pKa = 3.74FRR41 pKa = 11.84EE42 pKa = 4.29RR43 pKa = 11.84EE44 pKa = 4.04ARR46 pKa = 11.84LSSTQLARR54 pKa = 11.84EE55 pKa = 4.54FWARR59 pKa = 11.84LATFGDD65 pKa = 3.81AVSEE69 pKa = 4.23VFRR72 pKa = 11.84GGFAGGSQAMPFVRR86 pKa = 11.84RR87 pKa = 11.84SGLEE91 pKa = 3.79EE92 pKa = 3.42FRR94 pKa = 11.84EE95 pKa = 4.01RR96 pKa = 11.84HH97 pKa = 5.82DD98 pKa = 4.67PISRR102 pKa = 11.84LMYY105 pKa = 9.59SLVAVRR111 pKa = 11.84YY112 pKa = 8.63AKK114 pKa = 11.02AMLGARR120 pKa = 11.84KK121 pKa = 10.31SMVV124 pKa = 2.77

Molecular weight: 14.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

12620

36

1280

233.7

25.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.067 ± 0.637

0.998 ± 0.188

5.412 ± 0.299

5.127 ± 0.416

2.932 ± 0.213

7.956 ± 0.415

1.688 ± 0.157

4.984 ± 0.281

3.463 ± 0.244

7.52 ± 0.299

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.536 ± 0.128

4.319 ± 0.325

4.921 ± 0.301

5.594 ± 0.548

5.943 ± 0.409

6.807 ± 0.406

6.727 ± 0.352

6.268 ± 0.319

1.292 ± 0.217

2.448 ± 0.185
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski