Amino acid dipepetide frequency for Ralstonia phage RSK1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.019AlaAla: 19.019 ± 1.981
1.426AlaCys: 1.426 ± 0.408
6.657AlaAsp: 6.657 ± 0.87
7.766AlaGlu: 7.766 ± 1.426
3.725AlaPhe: 3.725 ± 0.567
9.192AlaGly: 9.192 ± 1.033
2.06AlaHis: 2.06 ± 0.442
6.023AlaIle: 6.023 ± 0.663
4.042AlaLys: 4.042 ± 0.603
9.985AlaLeu: 9.985 ± 0.973
3.17AlaMet: 3.17 ± 0.505
5.785AlaAsn: 5.785 ± 0.822
6.657AlaPro: 6.657 ± 0.806
8.321AlaGln: 8.321 ± 1.516
7.845AlaArg: 7.845 ± 1.017
7.845AlaSer: 7.845 ± 0.873
8.004AlaThr: 8.004 ± 0.833
7.925AlaVal: 7.925 ± 0.905
2.14AlaTrp: 2.14 ± 0.389
3.091AlaTyr: 3.091 ± 0.547
0.0AlaXaa: 0.0 ± 0.0
Cys
0.872CysAla: 0.872 ± 0.252
0.079CysCys: 0.079 ± 0.072
0.713CysAsp: 0.713 ± 0.243
0.634CysGlu: 0.634 ± 0.243
0.158CysPhe: 0.158 ± 0.104
0.634CysGly: 0.634 ± 0.249
0.079CysHis: 0.079 ± 0.072
0.158CysIle: 0.158 ± 0.124
0.396CysLys: 0.396 ± 0.163
0.713CysLeu: 0.713 ± 0.25
0.158CysMet: 0.158 ± 0.108
0.475CysAsn: 0.475 ± 0.205
0.238CysPro: 0.238 ± 0.143
0.079CysGln: 0.079 ± 0.079
0.951CysArg: 0.951 ± 0.335
0.872CysSer: 0.872 ± 0.338
0.872CysThr: 0.872 ± 0.33
1.03CysVal: 1.03 ± 0.391
0.396CysTrp: 0.396 ± 0.156
0.475CysTyr: 0.475 ± 0.248
0.0CysXaa: 0.0 ± 0.0
Asp
7.766AspAla: 7.766 ± 1.13
0.634AspCys: 0.634 ± 0.231
4.042AspAsp: 4.042 ± 0.856
3.725AspGlu: 3.725 ± 0.787
1.823AspPhe: 1.823 ± 0.614
4.438AspGly: 4.438 ± 0.729
1.03AspHis: 1.03 ± 0.3
2.219AspIle: 2.219 ± 0.527
1.426AspLys: 1.426 ± 0.347
4.517AspLeu: 4.517 ± 0.698
1.506AspMet: 1.506 ± 0.367
2.298AspAsn: 2.298 ± 0.51
2.932AspPro: 2.932 ± 0.379
2.457AspGln: 2.457 ± 0.536
2.377AspArg: 2.377 ± 0.566
3.091AspSer: 3.091 ± 0.487
2.774AspThr: 2.774 ± 0.556
3.328AspVal: 3.328 ± 0.564
0.713AspTrp: 0.713 ± 0.213
1.03AspTyr: 1.03 ± 0.355
0.0AspXaa: 0.0 ± 0.0
Glu
6.419GluAla: 6.419 ± 1.131
0.634GluCys: 0.634 ± 0.242
2.14GluAsp: 2.14 ± 0.48
3.328GluGlu: 3.328 ± 0.727
1.585GluPhe: 1.585 ± 0.415
2.932GluGly: 2.932 ± 0.498
1.664GluHis: 1.664 ± 0.43
3.249GluIle: 3.249 ± 0.548
2.298GluLys: 2.298 ± 0.58
3.645GluLeu: 3.645 ± 0.569
1.664GluMet: 1.664 ± 0.369
1.902GluAsn: 1.902 ± 0.409
2.06GluPro: 2.06 ± 0.472
2.853GluGln: 2.853 ± 0.518
4.596GluArg: 4.596 ± 0.796
3.091GluSer: 3.091 ± 0.651
2.932GluThr: 2.932 ± 0.516
2.615GluVal: 2.615 ± 0.546
0.475GluTrp: 0.475 ± 0.221
1.189GluTyr: 1.189 ± 0.377
0.0GluXaa: 0.0 ± 0.0
Phe
3.249PheAla: 3.249 ± 0.514
0.158PheCys: 0.158 ± 0.114
2.377PheAsp: 2.377 ± 0.356
2.06PheGlu: 2.06 ± 0.416
0.634PhePhe: 0.634 ± 0.217
3.17PheGly: 3.17 ± 0.546
0.317PheHis: 0.317 ± 0.154
1.268PheIle: 1.268 ± 0.422
1.109PheLys: 1.109 ± 0.274
1.585PheLeu: 1.585 ± 0.267
0.792PheMet: 0.792 ± 0.21
1.743PheAsn: 1.743 ± 0.509
1.664PhePro: 1.664 ± 0.402
1.426PheGln: 1.426 ± 0.304
1.664PheArg: 1.664 ± 0.443
1.426PheSer: 1.426 ± 0.387
2.06PheThr: 2.06 ± 0.407
1.585PheVal: 1.585 ± 0.417
0.396PheTrp: 0.396 ± 0.168
0.634PheTyr: 0.634 ± 0.222
0.0PheXaa: 0.0 ± 0.0
Gly
10.46GlyAla: 10.46 ± 1.361
0.555GlyCys: 0.555 ± 0.232
3.566GlyAsp: 3.566 ± 0.601
3.487GlyGlu: 3.487 ± 0.459
1.981GlyPhe: 1.981 ± 0.376
6.894GlyGly: 6.894 ± 1.241
0.951GlyHis: 0.951 ± 0.254
3.011GlyIle: 3.011 ± 0.458
3.725GlyLys: 3.725 ± 0.706
5.864GlyLeu: 5.864 ± 0.725
1.902GlyMet: 1.902 ± 0.469
3.566GlyAsn: 3.566 ± 0.785
3.17GlyPro: 3.17 ± 0.565
4.755GlyGln: 4.755 ± 0.986
4.121GlyArg: 4.121 ± 0.463
5.626GlySer: 5.626 ± 0.859
7.291GlyThr: 7.291 ± 1.602
4.596GlyVal: 4.596 ± 0.736
1.347GlyTrp: 1.347 ± 0.396
2.694GlyTyr: 2.694 ± 0.417
0.0GlyXaa: 0.0 ± 0.0
His
1.506HisAla: 1.506 ± 0.367
0.158HisCys: 0.158 ± 0.122
1.347HisAsp: 1.347 ± 0.342
0.951HisGlu: 0.951 ± 0.367
0.475HisPhe: 0.475 ± 0.174
1.109HisGly: 1.109 ± 0.251
0.396HisHis: 0.396 ± 0.221
1.268HisIle: 1.268 ± 0.297
0.396HisLys: 0.396 ± 0.212
1.189HisLeu: 1.189 ± 0.39
0.634HisMet: 0.634 ± 0.235
0.555HisAsn: 0.555 ± 0.3
0.713HisPro: 0.713 ± 0.224
1.109HisGln: 1.109 ± 0.26
1.426HisArg: 1.426 ± 0.4
0.872HisSer: 0.872 ± 0.321
0.872HisThr: 0.872 ± 0.251
1.268HisVal: 1.268 ± 0.335
0.317HisTrp: 0.317 ± 0.199
0.317HisTyr: 0.317 ± 0.141
0.0HisXaa: 0.0 ± 0.0
Ile
6.657IleAla: 6.657 ± 0.625
0.317IleCys: 0.317 ± 0.141
3.328IleAsp: 3.328 ± 0.49
3.011IleGlu: 3.011 ± 0.517
1.743IlePhe: 1.743 ± 0.442
4.042IleGly: 4.042 ± 0.767
0.872IleHis: 0.872 ± 0.264
1.823IleIle: 1.823 ± 0.49
2.774IleLys: 2.774 ± 0.427
2.615IleLeu: 2.615 ± 0.497
0.634IleMet: 0.634 ± 0.208
1.981IleAsn: 1.981 ± 0.432
3.011IlePro: 3.011 ± 0.605
2.457IleGln: 2.457 ± 0.552
2.694IleArg: 2.694 ± 0.581
3.17IleSer: 3.17 ± 0.536
3.328IleThr: 3.328 ± 0.63
3.17IleVal: 3.17 ± 0.611
0.872IleTrp: 0.872 ± 0.304
1.347IleTyr: 1.347 ± 0.381
0.0IleXaa: 0.0 ± 0.0
Lys
4.438LysAla: 4.438 ± 0.667
0.555LysCys: 0.555 ± 0.208
1.664LysAsp: 1.664 ± 0.372
1.902LysGlu: 1.902 ± 0.566
1.189LysPhe: 1.189 ± 0.257
1.981LysGly: 1.981 ± 0.318
0.792LysHis: 0.792 ± 0.273
1.823LysIle: 1.823 ± 0.476
1.03LysLys: 1.03 ± 0.268
3.408LysLeu: 3.408 ± 0.531
1.189LysMet: 1.189 ± 0.285
1.347LysAsn: 1.347 ± 0.321
2.06LysPro: 2.06 ± 0.54
1.981LysGln: 1.981 ± 0.389
2.615LysArg: 2.615 ± 0.479
1.902LysSer: 1.902 ± 0.568
1.981LysThr: 1.981 ± 0.435
1.823LysVal: 1.823 ± 0.342
0.396LysTrp: 0.396 ± 0.169
0.555LysTyr: 0.555 ± 0.266
0.0LysXaa: 0.0 ± 0.0
Leu
8.479LeuAla: 8.479 ± 0.934
0.396LeuCys: 0.396 ± 0.182
4.755LeuAsp: 4.755 ± 0.806
3.566LeuGlu: 3.566 ± 0.82
1.743LeuPhe: 1.743 ± 0.326
6.736LeuGly: 6.736 ± 0.849
0.792LeuHis: 0.792 ± 0.246
3.328LeuIle: 3.328 ± 0.571
2.774LeuLys: 2.774 ± 0.469
5.389LeuLeu: 5.389 ± 0.649
2.14LeuMet: 2.14 ± 0.313
3.804LeuAsn: 3.804 ± 0.624
2.853LeuPro: 2.853 ± 0.543
4.675LeuGln: 4.675 ± 0.558
5.309LeuArg: 5.309 ± 0.914
6.102LeuSer: 6.102 ± 0.767
5.151LeuThr: 5.151 ± 0.619
4.121LeuVal: 4.121 ± 0.622
0.951LeuTrp: 0.951 ± 0.356
2.14LeuTyr: 2.14 ± 0.354
0.0LeuXaa: 0.0 ± 0.0
Met
3.091MetAla: 3.091 ± 0.545
0.396MetCys: 0.396 ± 0.185
1.268MetAsp: 1.268 ± 0.289
1.109MetGlu: 1.109 ± 0.3
0.396MetPhe: 0.396 ± 0.203
1.664MetGly: 1.664 ± 0.324
0.317MetHis: 0.317 ± 0.142
0.634MetIle: 0.634 ± 0.241
1.109MetLys: 1.109 ± 0.314
2.377MetLeu: 2.377 ± 0.47
0.238MetMet: 0.238 ± 0.148
1.03MetAsn: 1.03 ± 0.271
1.506MetPro: 1.506 ± 0.437
1.902MetGln: 1.902 ± 0.356
2.14MetArg: 2.14 ± 0.424
1.902MetSer: 1.902 ± 0.37
2.377MetThr: 2.377 ± 0.429
1.189MetVal: 1.189 ± 0.311
0.317MetTrp: 0.317 ± 0.203
0.396MetTyr: 0.396 ± 0.179
0.0MetXaa: 0.0 ± 0.0
Asn
7.132AsnAla: 7.132 ± 0.901
0.317AsnCys: 0.317 ± 0.215
1.902AsnAsp: 1.902 ± 0.439
1.347AsnGlu: 1.347 ± 0.283
1.03AsnPhe: 1.03 ± 0.275
4.755AsnGly: 4.755 ± 0.993
0.317AsnHis: 0.317 ± 0.153
1.902AsnIle: 1.902 ± 0.454
1.03AsnLys: 1.03 ± 0.325
3.17AsnLeu: 3.17 ± 0.51
0.872AsnMet: 0.872 ± 0.3
1.823AsnAsn: 1.823 ± 0.438
3.249AsnPro: 3.249 ± 0.575
3.011AsnGln: 3.011 ± 0.519
1.743AsnArg: 1.743 ± 0.355
3.328AsnSer: 3.328 ± 0.68
2.694AsnThr: 2.694 ± 0.573
2.377AsnVal: 2.377 ± 0.571
0.317AsnTrp: 0.317 ± 0.164
0.872AsnTyr: 0.872 ± 0.312
0.0AsnXaa: 0.0 ± 0.0
Pro
6.577ProAla: 6.577 ± 1.02
0.634ProCys: 0.634 ± 0.257
2.774ProAsp: 2.774 ± 0.489
3.249ProGlu: 3.249 ± 0.727
1.506ProPhe: 1.506 ± 0.325
3.566ProGly: 3.566 ± 0.618
1.189ProHis: 1.189 ± 0.434
2.853ProIle: 2.853 ± 0.45
1.585ProLys: 1.585 ± 0.352
3.249ProLeu: 3.249 ± 0.6
1.347ProMet: 1.347 ± 0.29
1.981ProAsn: 1.981 ± 0.424
1.823ProPro: 1.823 ± 0.393
2.457ProGln: 2.457 ± 0.481
2.06ProArg: 2.06 ± 0.495
3.645ProSer: 3.645 ± 0.671
3.645ProThr: 3.645 ± 0.689
3.249ProVal: 3.249 ± 0.436
0.396ProTrp: 0.396 ± 0.175
1.426ProTyr: 1.426 ± 0.255
0.0ProXaa: 0.0 ± 0.0
Gln
7.053GlnAla: 7.053 ± 1.173
0.713GlnCys: 0.713 ± 0.199
1.823GlnAsp: 1.823 ± 0.396
1.664GlnGlu: 1.664 ± 0.527
2.298GlnPhe: 2.298 ± 0.344
3.328GlnGly: 3.328 ± 0.607
1.109GlnHis: 1.109 ± 0.31
4.359GlnIle: 4.359 ± 0.487
1.189GlnLys: 1.189 ± 0.255
4.359GlnLeu: 4.359 ± 0.596
1.426GlnMet: 1.426 ± 0.332
2.298GlnAsn: 2.298 ± 0.499
3.091GlnPro: 3.091 ± 0.447
5.23GlnGln: 5.23 ± 1.183
3.487GlnArg: 3.487 ± 0.638
4.042GlnSer: 4.042 ± 0.671
3.17GlnThr: 3.17 ± 0.504
3.011GlnVal: 3.011 ± 0.529
0.951GlnTrp: 0.951 ± 0.222
1.268GlnTyr: 1.268 ± 0.344
0.0GlnXaa: 0.0 ± 0.0
Arg
7.608ArgAla: 7.608 ± 0.946
0.792ArgCys: 0.792 ± 0.333
2.853ArgAsp: 2.853 ± 0.515
3.566ArgGlu: 3.566 ± 0.754
1.743ArgPhe: 1.743 ± 0.384
3.328ArgGly: 3.328 ± 0.509
1.268ArgHis: 1.268 ± 0.407
3.408ArgIle: 3.408 ± 0.492
2.06ArgLys: 2.06 ± 0.423
5.23ArgLeu: 5.23 ± 0.892
1.981ArgMet: 1.981 ± 0.508
2.219ArgAsn: 2.219 ± 0.353
1.743ArgPro: 1.743 ± 0.378
2.615ArgGln: 2.615 ± 0.553
3.962ArgArg: 3.962 ± 0.886
3.645ArgSer: 3.645 ± 0.786
3.408ArgThr: 3.408 ± 0.456
5.151ArgVal: 5.151 ± 0.595
0.872ArgTrp: 0.872 ± 0.392
1.981ArgTyr: 1.981 ± 0.47
0.0ArgXaa: 0.0 ± 0.0
Ser
10.302SerAla: 10.302 ± 1.274
0.475SerCys: 0.475 ± 0.209
3.408SerAsp: 3.408 ± 0.529
2.932SerGlu: 2.932 ± 0.629
1.506SerPhe: 1.506 ± 0.33
6.974SerGly: 6.974 ± 0.921
1.109SerHis: 1.109 ± 0.305
3.566SerIle: 3.566 ± 0.599
1.981SerLys: 1.981 ± 0.385
4.517SerLeu: 4.517 ± 0.633
1.347SerMet: 1.347 ± 0.288
2.377SerAsn: 2.377 ± 0.639
3.249SerPro: 3.249 ± 0.698
3.408SerGln: 3.408 ± 0.605
3.328SerArg: 3.328 ± 0.571
6.34SerSer: 6.34 ± 0.933
4.042SerThr: 4.042 ± 0.581
5.151SerVal: 5.151 ± 0.777
0.555SerTrp: 0.555 ± 0.175
1.506SerTyr: 1.506 ± 0.482
0.0SerXaa: 0.0 ± 0.0
Thr
7.766ThrAla: 7.766 ± 1.119
0.475ThrCys: 0.475 ± 0.187
3.962ThrAsp: 3.962 ± 0.642
2.694ThrGlu: 2.694 ± 0.496
2.14ThrPhe: 2.14 ± 0.486
6.894ThrGly: 6.894 ± 1.046
0.951ThrHis: 0.951 ± 0.232
4.121ThrIle: 4.121 ± 0.815
2.219ThrLys: 2.219 ± 0.392
5.626ThrLeu: 5.626 ± 0.69
1.585ThrMet: 1.585 ± 0.316
3.249ThrAsn: 3.249 ± 0.519
4.438ThrPro: 4.438 ± 0.51
2.774ThrGln: 2.774 ± 0.572
2.853ThrArg: 2.853 ± 0.442
3.408ThrSer: 3.408 ± 0.608
5.072ThrThr: 5.072 ± 0.922
5.23ThrVal: 5.23 ± 0.818
0.396ThrTrp: 0.396 ± 0.194
1.426ThrTyr: 1.426 ± 0.311
0.0ThrXaa: 0.0 ± 0.0
Val
8.242ValAla: 8.242 ± 0.806
0.475ValCys: 0.475 ± 0.198
2.774ValAsp: 2.774 ± 0.592
2.853ValGlu: 2.853 ± 0.46
2.298ValPhe: 2.298 ± 0.526
5.785ValGly: 5.785 ± 0.66
0.872ValHis: 0.872 ± 0.246
2.932ValIle: 2.932 ± 0.625
2.219ValLys: 2.219 ± 0.404
4.755ValLeu: 4.755 ± 0.503
1.902ValMet: 1.902 ± 0.37
3.091ValAsn: 3.091 ± 0.619
3.17ValPro: 3.17 ± 0.467
2.694ValGln: 2.694 ± 0.475
3.566ValArg: 3.566 ± 0.596
5.151ValSer: 5.151 ± 0.851
4.596ValThr: 4.596 ± 0.721
3.645ValVal: 3.645 ± 0.65
0.317ValTrp: 0.317 ± 0.166
1.347ValTyr: 1.347 ± 0.392
0.0ValXaa: 0.0 ± 0.0
Trp
1.109TrpAla: 1.109 ± 0.328
0.317TrpCys: 0.317 ± 0.175
0.951TrpAsp: 0.951 ± 0.317
0.555TrpGlu: 0.555 ± 0.265
0.713TrpPhe: 0.713 ± 0.244
0.634TrpGly: 0.634 ± 0.23
0.475TrpHis: 0.475 ± 0.212
0.951TrpIle: 0.951 ± 0.251
0.555TrpLys: 0.555 ± 0.203
1.268TrpLeu: 1.268 ± 0.367
0.158TrpMet: 0.158 ± 0.115
0.396TrpAsn: 0.396 ± 0.167
0.317TrpPro: 0.317 ± 0.18
0.713TrpGln: 0.713 ± 0.223
0.792TrpArg: 0.792 ± 0.278
0.555TrpSer: 0.555 ± 0.208
1.109TrpThr: 1.109 ± 0.294
0.713TrpVal: 0.713 ± 0.279
0.238TrpTrp: 0.238 ± 0.124
0.396TrpTyr: 0.396 ± 0.169
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.932TyrAla: 2.932 ± 0.496
0.475TyrCys: 0.475 ± 0.2
1.823TyrAsp: 1.823 ± 0.303
0.872TyrGlu: 0.872 ± 0.253
0.634TyrPhe: 0.634 ± 0.223
1.506TyrGly: 1.506 ± 0.361
0.317TyrHis: 0.317 ± 0.134
0.951TyrIle: 0.951 ± 0.282
0.713TyrLys: 0.713 ± 0.189
1.743TyrLeu: 1.743 ± 0.494
0.634TyrMet: 0.634 ± 0.222
1.268TyrAsn: 1.268 ± 0.372
1.268TyrPro: 1.268 ± 0.392
1.03TyrGln: 1.03 ± 0.287
1.902TyrArg: 1.902 ± 0.317
2.06TyrSer: 2.06 ± 0.454
1.902TyrThr: 1.902 ± 0.569
1.506TyrVal: 1.506 ± 0.364
0.555TyrTrp: 0.555 ± 0.264
0.396TyrTyr: 0.396 ± 0.165
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 54 proteins (12620 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski