Erythrobacter litoralis
Average proteome isoelectric point is 5.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2981 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A074MEZ3|A0A074MEZ3_9SPHN Cystathionine beta-lyase OS=Erythrobacter litoralis OX=39960 GN=EH32_12045 PE=3 SV=1
MM1 pKa = 7.52 ALAAAPAFAAPAGEE15 pKa = 4.32 EE16 pKa = 4.36 LPHH19 pKa = 6.68 EE20 pKa = 5.06 GPDD23 pKa = 3.51 AQDD26 pKa = 3.13 PVIPIKK32 pKa = 10.62 ARR34 pKa = 11.84 SGIGEE39 pKa = 3.86 GRR41 pKa = 11.84 EE42 pKa = 4.21 ANNTASAISGQGFTITANATLASEE66 pKa = 4.18 YY67 pKa = 10.36 RR68 pKa = 11.84 FRR70 pKa = 11.84 GVDD73 pKa = 3.55 LSGGDD78 pKa = 3.52 PTVQGGIDD86 pKa = 3.87 LFHH89 pKa = 7.31 DD90 pKa = 3.34 TGFYY94 pKa = 10.67 AGTFASTLDD103 pKa = 4.5 DD104 pKa = 3.58 EE105 pKa = 4.89 TTGYY109 pKa = 10.93 GGLEE113 pKa = 3.93 LDD115 pKa = 6.25 FYY117 pKa = 11.28 AGWSGDD123 pKa = 3.58 VAEE126 pKa = 5.1 GLSANLGVIAYY137 pKa = 6.92 TFPDD141 pKa = 3.69 APEE144 pKa = 4.87 GDD146 pKa = 3.81 FDD148 pKa = 4.26 YY149 pKa = 11.62 LEE151 pKa = 5.25 LYY153 pKa = 10.62 GSVGFTLGPAQATVGAAWDD172 pKa = 3.62 VGEE175 pKa = 5.16 DD176 pKa = 3.54 GLRR179 pKa = 11.84 FGGLRR184 pKa = 11.84 RR185 pKa = 11.84 DD186 pKa = 3.47 NLYY189 pKa = 11.11 VYY191 pKa = 9.72 TDD193 pKa = 3.86 LSAGVPGTPVTVNAHH208 pKa = 6.45 LGYY211 pKa = 9.31 TDD213 pKa = 3.74 GAIDD217 pKa = 3.64 FAGEE221 pKa = 4.27 SVSFDD226 pKa = 3.05 WSLGADD232 pKa = 3.2 WAIRR236 pKa = 11.84 DD237 pKa = 3.92 TPLVLGVAYY246 pKa = 10.54 VDD248 pKa = 3.96 AADD251 pKa = 4.92 DD252 pKa = 3.86 VAPPGAFNPTSGTILGTLTAYY273 pKa = 9.83 FF274 pKa = 4.01
Molecular weight: 28.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.713
IPC2_protein 3.681
IPC_protein 3.694
Toseland 3.465
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.643
Rodwell 3.516
Grimsley 3.376
Solomon 3.681
Lehninger 3.643
Nozaki 3.808
DTASelect 4.062
Thurlkill 3.528
EMBOSS 3.656
Sillero 3.821
Patrickios 0.807
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.732
Protein with the highest isoelectric point:
>tr|A0A074MC34|A0A074MC34_9SPHN Rieske domain-containing protein OS=Erythrobacter litoralis OX=39960 GN=EH32_01055 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.24 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.93 GFFARR21 pKa = 11.84 KK22 pKa = 7.42 ATPGGRR28 pKa = 11.84 KK29 pKa = 8.05 VLRR32 pKa = 11.84 LRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.04 KK41 pKa = 10.54 LSAA44 pKa = 4.03
Molecular weight: 5.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.525
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.056
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.735
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.554
Sillero 13.042
Patrickios 12.457
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.203
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2981
0
2981
948914
41
2739
318.3
34.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.05 ± 0.066
0.819 ± 0.013
6.074 ± 0.039
6.917 ± 0.042
3.7 ± 0.032
9.061 ± 0.038
1.902 ± 0.024
4.927 ± 0.033
2.884 ± 0.039
9.72 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.464 ± 0.025
2.441 ± 0.03
5.269 ± 0.031
2.841 ± 0.023
7.408 ± 0.05
5.104 ± 0.03
4.952 ± 0.033
6.964 ± 0.036
1.384 ± 0.02
2.119 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here