Natronomonas moolapensis (strain DSM 18674 / CECT 7526 / JCM 14361 / 8.8.11)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; Haloarculaceae; Natronomonas; Natronomonas moolapensis

Average proteome isoelectric point is 4.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2723 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1XQF6|M1XQF6_NATM8 Uncharacterized protein OS=Natronomonas moolapensis (strain DSM 18674 / CECT 7526 / JCM 14361 / 8.8.11) OX=268739 GN=Nmlp_2222 PE=4 SV=1
MM1 pKa = 7.12SAPWATWDD9 pKa = 4.46HH10 pKa = 5.97IVKK13 pKa = 9.85IDD15 pKa = 3.66PDD17 pKa = 3.47KK18 pKa = 11.11TLVEE22 pKa = 4.48GEE24 pKa = 4.35TFEE27 pKa = 5.45DD28 pKa = 4.11VCATGTDD35 pKa = 3.39ALEE38 pKa = 4.37IGGTTGMTEE47 pKa = 3.57EE48 pKa = 3.89KK49 pKa = 9.12MARR52 pKa = 11.84VVEE55 pKa = 4.12ATTAYY60 pKa = 9.95DD61 pKa = 3.43VPVYY65 pKa = 10.36IEE67 pKa = 4.16PSNVGAVVHH76 pKa = 6.85RR77 pKa = 11.84EE78 pKa = 3.75GLDD81 pKa = 3.57GYY83 pKa = 9.43FVPIVLNAGDD93 pKa = 4.14VFWVTGAHH101 pKa = 6.41KK102 pKa = 9.94EE103 pKa = 3.86WARR106 pKa = 11.84LDD108 pKa = 4.82SEE110 pKa = 4.56IDD112 pKa = 3.07WMATFPEE119 pKa = 5.14AYY121 pKa = 9.9LVLNPDD127 pKa = 3.45SSVANYY133 pKa = 9.47TEE135 pKa = 4.41ADD137 pKa = 3.57CDD139 pKa = 3.43IDD141 pKa = 4.25ADD143 pKa = 4.06EE144 pKa = 4.3VAAYY148 pKa = 10.53AEE150 pKa = 4.25VAEE153 pKa = 4.43RR154 pKa = 11.84MFGQRR159 pKa = 11.84IVYY162 pKa = 9.53VEE164 pKa = 3.76YY165 pKa = 10.78SGTFGDD171 pKa = 4.16PEE173 pKa = 4.23MVQAATDD180 pKa = 3.74AVEE183 pKa = 4.3EE184 pKa = 4.2TSVFYY189 pKa = 11.03GGGIHH194 pKa = 7.59DD195 pKa = 4.88YY196 pKa = 11.03EE197 pKa = 5.02SAHH200 pKa = 5.52QMGRR204 pKa = 11.84HH205 pKa = 6.38ADD207 pKa = 3.92TIVVGDD213 pKa = 4.11LVHH216 pKa = 7.59DD217 pKa = 4.36EE218 pKa = 4.53GVDD221 pKa = 3.65AVAEE225 pKa = 4.3TVEE228 pKa = 4.57GVADD232 pKa = 4.12ANSEE236 pKa = 4.33PIEE239 pKa = 4.27GAA241 pKa = 3.23

Molecular weight: 26.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1XP12|M1XP12_NATM8 Orotate phosphoribosyltransferase OS=Natronomonas moolapensis (strain DSM 18674 / CECT 7526 / JCM 14361 / 8.8.11) OX=268739 GN=pyrE1 PE=3 SV=1
MM1 pKa = 7.48KK2 pKa = 10.32KK3 pKa = 10.3NVGGTDD9 pKa = 2.72RR10 pKa = 11.84VVRR13 pKa = 11.84LVLGSALIVAGIAGYY28 pKa = 10.76AGLLPLAAGSLPQALTAVVVFVLGAVLLATGLIRR62 pKa = 11.84RR63 pKa = 11.84CPLNRR68 pKa = 11.84LVGLDD73 pKa = 3.61TYY75 pKa = 11.81

Molecular weight: 7.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2723

0

2723

809592

31

4220

297.3

32.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.202 ± 0.076

0.767 ± 0.017

8.228 ± 0.052

8.839 ± 0.071

3.197 ± 0.028

8.898 ± 0.055

1.941 ± 0.023

4.101 ± 0.03

1.863 ± 0.028

8.699 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.654 ± 0.022

2.307 ± 0.039

4.627 ± 0.034

2.213 ± 0.028

6.909 ± 0.052

5.674 ± 0.057

6.388 ± 0.056

8.861 ± 0.047

0.993 ± 0.018

2.641 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski