Methanobacterium sp. MB1

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Methanomada group; Methanobacteria; Methanobacteriales; Methanobacteriaceae; Methanobacterium; unclassified Methanobacterium

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2013 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U6EER3|U6EER3_9EURY CARDB domain-containing protein OS=Methanobacterium sp. MB1 OX=1379702 GN=MBMB1_1451 PE=4 SV=1
MM1 pKa = 7.22NKK3 pKa = 9.56DD4 pKa = 3.4HH5 pKa = 7.14KK6 pKa = 11.41LMGGLIPVILILCVGLYY23 pKa = 9.69GLPVLGSTADD33 pKa = 4.2PINAVPYY40 pKa = 10.91NDD42 pKa = 4.15TNDD45 pKa = 3.53TNTTVTNDD53 pKa = 3.26TVASDD58 pKa = 3.58EE59 pKa = 4.81SNVVNDD65 pKa = 4.28TDD67 pKa = 3.9STQPNSQSYY76 pKa = 10.22SRR78 pKa = 11.84NTNYY82 pKa = 7.75YY83 pKa = 8.06TPNRR87 pKa = 11.84GNTPTPAPAPAPAPAPAPEE106 pKa = 4.6PAPAPEE112 pKa = 4.48PEE114 pKa = 4.3PTPSEE119 pKa = 4.09

Molecular weight: 12.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U6EE34|U6EE34_9EURY DUF89 domain-containing protein OS=Methanobacterium sp. MB1 OX=1379702 GN=MBMB1_1533 PE=4 SV=1
MM1 pKa = 6.76YY2 pKa = 10.07RR3 pKa = 11.84HH4 pKa = 7.15RR5 pKa = 11.84ILQILVMYY13 pKa = 9.59SEE15 pKa = 5.54IMLQQTGCWKK25 pKa = 10.04PRR27 pKa = 11.84TKK29 pKa = 10.46QLGSKK34 pKa = 8.54EE35 pKa = 4.18PRR37 pKa = 11.84SSHH40 pKa = 6.54PSPIKK45 pKa = 10.12QVFYY49 pKa = 10.73SYY51 pKa = 10.88PKK53 pKa = 8.75SCLFSGEE60 pKa = 4.14TSGLDD65 pKa = 3.32AFSLYY70 pKa = 10.86NLARR74 pKa = 11.84SCPAMPYY81 pKa = 8.45QTTGRR86 pKa = 11.84PEE88 pKa = 3.92APTARR93 pKa = 11.84SSRR96 pKa = 11.84TGATFPSDD104 pKa = 3.25NKK106 pKa = 10.24TIPLDD111 pKa = 3.61SNQPVSRR118 pKa = 11.84RR119 pKa = 11.84SKK121 pKa = 9.39PSSRR125 pKa = 11.84SPLMGEE131 pKa = 4.34QPHH134 pKa = 6.43PWVMLHH140 pKa = 5.54TQDD143 pKa = 2.78GKK145 pKa = 10.6NRR147 pKa = 11.84HH148 pKa = 6.03RR149 pKa = 11.84SSKK152 pKa = 8.67PQGRR156 pKa = 11.84YY157 pKa = 9.69GLLPATTQLSPRR169 pKa = 3.88

Molecular weight: 19.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2013

0

2013

559669

29

2654

278.0

31.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.44 ± 0.064

1.196 ± 0.032

5.62 ± 0.04

7.652 ± 0.074

3.88 ± 0.046

7.103 ± 0.047

1.897 ± 0.023

8.352 ± 0.063

7.095 ± 0.059

9.564 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.827 ± 0.021

4.488 ± 0.053

4.08 ± 0.032

3.145 ± 0.033

4.1 ± 0.038

5.942 ± 0.04

5.226 ± 0.052

7.185 ± 0.045

0.806 ± 0.019

3.402 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski