Amino acid dipepetide frequency for Methanobacterium sp. MB1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.103AlaAla: 5.103 ± 0.137
0.822AlaCys: 0.822 ± 0.043
3.402AlaAsp: 3.402 ± 0.084
4.399AlaGlu: 4.399 ± 0.105
2.407AlaPhe: 2.407 ± 0.078
4.833AlaGly: 4.833 ± 0.126
1.199AlaHis: 1.199 ± 0.054
5.316AlaIle: 5.316 ± 0.109
3.495AlaLys: 3.495 ± 0.085
7.008AlaLeu: 7.008 ± 0.132
1.914AlaMet: 1.914 ± 0.065
2.291AlaAsn: 2.291 ± 0.063
2.157AlaPro: 2.157 ± 0.07
2.357AlaGln: 2.357 ± 0.074
3.1AlaArg: 3.1 ± 0.081
3.813AlaSer: 3.813 ± 0.084
3.261AlaThr: 3.261 ± 0.081
5.092AlaVal: 5.092 ± 0.103
0.479AlaTrp: 0.479 ± 0.034
1.957AlaTyr: 1.957 ± 0.064
0.0AlaXaa: 0.0 ± 0.0
Cys
0.708CysAla: 0.708 ± 0.038
0.188CysCys: 0.188 ± 0.023
0.618CysAsp: 0.618 ± 0.034
0.686CysGlu: 0.686 ± 0.042
0.413CysPhe: 0.413 ± 0.028
1.406CysGly: 1.406 ± 0.059
0.295CysHis: 0.295 ± 0.027
0.775CysIle: 0.775 ± 0.044
0.6CysLys: 0.6 ± 0.037
0.99CysLeu: 0.99 ± 0.043
0.279CysMet: 0.279 ± 0.024
0.5CysAsn: 0.5 ± 0.028
0.974CysPro: 0.974 ± 0.072
0.504CysGln: 0.504 ± 0.034
0.509CysArg: 0.509 ± 0.03
0.704CysSer: 0.704 ± 0.04
0.575CysThr: 0.575 ± 0.039
0.758CysVal: 0.758 ± 0.044
0.075CysTrp: 0.075 ± 0.011
0.398CysTyr: 0.398 ± 0.027
0.0CysXaa: 0.0 ± 0.0
Asp
3.491AspAla: 3.491 ± 0.074
0.652AspCys: 0.652 ± 0.035
2.93AspAsp: 2.93 ± 0.078
4.703AspGlu: 4.703 ± 0.111
2.43AspPhe: 2.43 ± 0.071
3.35AspGly: 3.35 ± 0.092
1.092AspHis: 1.092 ± 0.043
5.039AspIle: 5.039 ± 0.095
3.772AspLys: 3.772 ± 0.085
6.084AspLeu: 6.084 ± 0.103
1.692AspMet: 1.692 ± 0.062
2.269AspAsn: 2.269 ± 0.067
2.68AspPro: 2.68 ± 0.067
1.562AspGln: 1.562 ± 0.055
1.96AspArg: 1.96 ± 0.053
2.718AspSer: 2.718 ± 0.068
2.389AspThr: 2.389 ± 0.062
4.517AspVal: 4.517 ± 0.086
0.495AspTrp: 0.495 ± 0.028
2.378AspTyr: 2.378 ± 0.075
0.0AspXaa: 0.0 ± 0.0
Glu
4.69GluAla: 4.69 ± 0.098
0.777GluCys: 0.777 ± 0.039
4.749GluAsp: 4.749 ± 0.103
7.29GluGlu: 7.29 ± 0.149
2.782GluPhe: 2.782 ± 0.06
4.928GluGly: 4.928 ± 0.098
1.22GluHis: 1.22 ± 0.048
6.859GluIle: 6.859 ± 0.124
6.926GluLys: 6.926 ± 0.12
7.008GluLeu: 7.008 ± 0.127
2.426GluMet: 2.426 ± 0.068
4.067GluAsn: 4.067 ± 0.087
2.012GluPro: 2.012 ± 0.066
1.619GluGln: 1.619 ± 0.052
3.016GluArg: 3.016 ± 0.075
3.883GluSer: 3.883 ± 0.093
3.817GluThr: 3.817 ± 0.088
5.523GluVal: 5.523 ± 0.096
0.507GluTrp: 0.507 ± 0.031
2.423GluTyr: 2.423 ± 0.07
0.0GluXaa: 0.0 ± 0.0
Phe
2.291PheAla: 2.291 ± 0.075
0.513PheCys: 0.513 ± 0.034
2.098PheAsp: 2.098 ± 0.057
2.2PheGlu: 2.2 ± 0.06
1.647PhePhe: 1.647 ± 0.063
2.791PheGly: 2.791 ± 0.072
0.786PheHis: 0.786 ± 0.038
3.339PheIle: 3.339 ± 0.091
2.655PheLys: 2.655 ± 0.081
4.117PheLeu: 4.117 ± 0.101
1.094PheMet: 1.094 ± 0.047
2.044PheAsn: 2.044 ± 0.061
1.544PhePro: 1.544 ± 0.057
1.546PheGln: 1.546 ± 0.061
1.517PheArg: 1.517 ± 0.051
2.464PheSer: 2.464 ± 0.071
2.139PheThr: 2.139 ± 0.063
2.253PheVal: 2.253 ± 0.068
0.313PheTrp: 0.313 ± 0.022
1.451PheTyr: 1.451 ± 0.05
0.0PheXaa: 0.0 ± 0.0
Gly
4.856GlyAla: 4.856 ± 0.124
0.995GlyCys: 0.995 ± 0.05
3.838GlyAsp: 3.838 ± 0.095
4.785GlyGlu: 4.785 ± 0.1
3.23GlyPhe: 3.23 ± 0.075
4.805GlyGly: 4.805 ± 0.106
1.344GlyHis: 1.344 ± 0.05
6.447GlyIle: 6.447 ± 0.13
5.133GlyLys: 5.133 ± 0.101
6.425GlyLeu: 6.425 ± 0.118
2.248GlyMet: 2.248 ± 0.07
2.905GlyAsn: 2.905 ± 0.088
2.164GlyPro: 2.164 ± 0.073
1.828GlyGln: 1.828 ± 0.059
2.87GlyArg: 2.87 ± 0.071
4.261GlySer: 4.261 ± 0.087
3.97GlyThr: 3.97 ± 0.089
5.66GlyVal: 5.66 ± 0.109
0.634GlyTrp: 0.634 ± 0.035
2.635GlyTyr: 2.635 ± 0.072
0.0GlyXaa: 0.0 ± 0.0
His
1.101HisAla: 1.101 ± 0.043
0.284HisCys: 0.284 ± 0.022
1.011HisAsp: 1.011 ± 0.047
1.24HisGlu: 1.24 ± 0.051
0.731HisPhe: 0.731 ± 0.035
1.428HisGly: 1.428 ± 0.053
0.609HisHis: 0.609 ± 0.033
1.315HisIle: 1.315 ± 0.056
0.997HisLys: 0.997 ± 0.044
1.892HisLeu: 1.892 ± 0.064
0.456HisMet: 0.456 ± 0.03
0.797HisAsn: 0.797 ± 0.039
1.213HisPro: 1.213 ± 0.052
0.768HisGln: 0.768 ± 0.04
0.908HisArg: 0.908 ± 0.043
1.192HisSer: 1.192 ± 0.046
0.927HisThr: 0.927 ± 0.043
1.186HisVal: 1.186 ± 0.047
0.175HisTrp: 0.175 ± 0.017
0.736HisTyr: 0.736 ± 0.031
0.0HisXaa: 0.0 ± 0.0
Ile
5.394IleAla: 5.394 ± 0.096
0.967IleCys: 0.967 ± 0.039
4.202IleAsp: 4.202 ± 0.093
5.4IleGlu: 5.4 ± 0.112
3.306IlePhe: 3.306 ± 0.101
5.627IleGly: 5.627 ± 0.115
1.596IleHis: 1.596 ± 0.052
7.476IleIle: 7.476 ± 0.156
6.011IleLys: 6.011 ± 0.113
8.535IleLeu: 8.535 ± 0.153
2.314IleMet: 2.314 ± 0.066
3.874IleAsn: 3.874 ± 0.088
4.086IlePro: 4.086 ± 0.085
2.46IleGln: 2.46 ± 0.068
3.323IleArg: 3.323 ± 0.087
5.335IleSer: 5.335 ± 0.11
4.935IleThr: 4.935 ± 0.09
5.301IleVal: 5.301 ± 0.099
0.572IleTrp: 0.572 ± 0.033
2.802IleTyr: 2.802 ± 0.076
0.0IleXaa: 0.0 ± 0.0
Lys
4.22LysAla: 4.22 ± 0.086
0.811LysCys: 0.811 ± 0.047
4.181LysAsp: 4.181 ± 0.089
6.261LysGlu: 6.261 ± 0.12
2.283LysPhe: 2.283 ± 0.056
4.565LysGly: 4.565 ± 0.095
1.197LysHis: 1.197 ± 0.048
6.525LysIle: 6.525 ± 0.109
6.143LysLys: 6.143 ± 0.134
5.887LysLeu: 5.887 ± 0.114
2.312LysMet: 2.312 ± 0.059
3.818LysAsn: 3.818 ± 0.101
2.462LysPro: 2.462 ± 0.065
1.717LysGln: 1.717 ± 0.055
2.929LysArg: 2.929 ± 0.077
4.024LysSer: 4.024 ± 0.09
3.917LysThr: 3.917 ± 0.092
4.783LysVal: 4.783 ± 0.099
0.518LysTrp: 0.518 ± 0.036
2.4LysTyr: 2.4 ± 0.065
0.0LysXaa: 0.0 ± 0.0
Leu
6.415LeuAla: 6.415 ± 0.123
0.956LeuCys: 0.956 ± 0.046
5.673LeuAsp: 5.673 ± 0.114
7.903LeuGlu: 7.903 ± 0.139
3.709LeuPhe: 3.709 ± 0.103
6.736LeuGly: 6.736 ± 0.146
1.635LeuHis: 1.635 ± 0.062
7.576LeuIle: 7.576 ± 0.166
8.201LeuLys: 8.201 ± 0.164
8.705LeuLeu: 8.705 ± 0.155
2.482LeuMet: 2.482 ± 0.067
4.467LeuAsn: 4.467 ± 0.123
3.727LeuPro: 3.727 ± 0.078
2.746LeuGln: 2.746 ± 0.078
3.722LeuArg: 3.722 ± 0.088
5.93LeuSer: 5.93 ± 0.113
4.848LeuThr: 4.848 ± 0.111
6.79LeuVal: 6.79 ± 0.117
0.708LeuTrp: 0.708 ± 0.036
2.705LeuTyr: 2.705 ± 0.079
0.0LeuXaa: 0.0 ± 0.0
Met
2.321MetAla: 2.321 ± 0.071
0.272MetCys: 0.272 ± 0.026
2.126MetAsp: 2.126 ± 0.062
2.661MetGlu: 2.661 ± 0.073
0.813MetPhe: 0.813 ± 0.041
2.4MetGly: 2.4 ± 0.079
0.407MetHis: 0.407 ± 0.027
2.153MetIle: 2.153 ± 0.062
2.107MetLys: 2.107 ± 0.065
2.049MetLeu: 2.049 ± 0.064
0.738MetMet: 0.738 ± 0.036
1.311MetAsn: 1.311 ± 0.048
0.915MetPro: 0.915 ± 0.042
0.72MetGln: 0.72 ± 0.036
1.017MetArg: 1.017 ± 0.043
1.488MetSer: 1.488 ± 0.042
1.24MetThr: 1.24 ± 0.043
2.675MetVal: 2.675 ± 0.073
0.204MetTrp: 0.204 ± 0.019
0.654MetTyr: 0.654 ± 0.035
0.0MetXaa: 0.0 ± 0.0
Asn
2.412AsnAla: 2.412 ± 0.079
0.556AsnCys: 0.556 ± 0.032
1.967AsnAsp: 1.967 ± 0.064
2.594AsnGlu: 2.594 ± 0.072
1.794AsnPhe: 1.794 ± 0.054
2.862AsnGly: 2.862 ± 0.074
0.877AsnHis: 0.877 ± 0.046
4.176AsnIle: 4.176 ± 0.096
2.8AsnLys: 2.8 ± 0.084
4.781AsnLeu: 4.781 ± 0.092
1.27AsnMet: 1.27 ± 0.047
2.035AsnAsn: 2.035 ± 0.091
2.73AsnPro: 2.73 ± 0.075
1.935AsnGln: 1.935 ± 0.074
1.88AsnArg: 1.88 ± 0.061
2.625AsnSer: 2.625 ± 0.085
2.392AsnThr: 2.392 ± 0.084
2.853AsnVal: 2.853 ± 0.082
0.47AsnTrp: 0.47 ± 0.028
1.865AsnTyr: 1.865 ± 0.064
0.0AsnXaa: 0.0 ± 0.0
Pro
2.267ProAla: 2.267 ± 0.069
0.482ProCys: 0.482 ± 0.029
2.571ProAsp: 2.571 ± 0.071
4.086ProGlu: 4.086 ± 0.092
1.64ProPhe: 1.64 ± 0.059
3.02ProGly: 3.02 ± 0.079
1.036ProHis: 1.036 ± 0.039
2.58ProIle: 2.58 ± 0.058
2.13ProLys: 2.13 ± 0.062
4.179ProLeu: 4.179 ± 0.094
0.842ProMet: 0.842 ± 0.037
1.472ProAsn: 1.472 ± 0.059
1.542ProPro: 1.542 ± 0.057
1.649ProGln: 1.649 ± 0.055
1.59ProArg: 1.59 ± 0.051
2.593ProSer: 2.593 ± 0.069
2.105ProThr: 2.105 ± 0.056
3.222ProVal: 3.222 ± 0.087
0.316ProTrp: 0.316 ± 0.025
1.479ProTyr: 1.479 ± 0.05
0.0ProXaa: 0.0 ± 0.0
Gln
2.21GlnAla: 2.21 ± 0.065
0.35GlnCys: 0.35 ± 0.026
1.903GlnAsp: 1.903 ± 0.063
3.073GlnGlu: 3.073 ± 0.08
1.061GlnPhe: 1.061 ± 0.044
2.155GlnGly: 2.155 ± 0.066
0.507GlnHis: 0.507 ± 0.03
2.519GlnIle: 2.519 ± 0.067
2.559GlnLys: 2.559 ± 0.078
2.68GlnLeu: 2.68 ± 0.08
0.938GlnMet: 0.938 ± 0.04
1.378GlnAsn: 1.378 ± 0.045
1.036GlnPro: 1.036 ± 0.044
0.945GlnGln: 0.945 ± 0.047
1.437GlnArg: 1.437 ± 0.048
1.588GlnSer: 1.588 ± 0.053
1.513GlnThr: 1.513 ± 0.052
2.303GlnVal: 2.303 ± 0.065
0.322GlnTrp: 0.322 ± 0.023
0.976GlnTyr: 0.976 ± 0.047
0.0GlnXaa: 0.0 ± 0.0
Arg
2.5ArgAla: 2.5 ± 0.077
0.491ArgCys: 0.491 ± 0.03
2.38ArgAsp: 2.38 ± 0.077
3.961ArgGlu: 3.961 ± 0.094
1.472ArgPhe: 1.472 ± 0.057
2.769ArgGly: 2.769 ± 0.067
0.597ArgHis: 0.597 ± 0.037
3.529ArgIle: 3.529 ± 0.103
3.411ArgLys: 3.411 ± 0.077
3.227ArgLeu: 3.227 ± 0.076
1.247ArgMet: 1.247 ± 0.047
1.778ArgAsn: 1.778 ± 0.06
1.295ArgPro: 1.295 ± 0.049
1.099ArgGln: 1.099 ± 0.047
2.085ArgArg: 2.085 ± 0.067
2.398ArgSer: 2.398 ± 0.065
1.953ArgThr: 1.953 ± 0.061
2.987ArgVal: 2.987 ± 0.067
0.345ArgTrp: 0.345 ± 0.026
1.474ArgTyr: 1.474 ± 0.05
0.0ArgXaa: 0.0 ± 0.0
Ser
3.438SerAla: 3.438 ± 0.086
0.69SerCys: 0.69 ± 0.033
2.936SerAsp: 2.936 ± 0.07
3.77SerGlu: 3.77 ± 0.091
2.648SerPhe: 2.648 ± 0.077
4.706SerGly: 4.706 ± 0.088
1.315SerHis: 1.315 ± 0.05
4.64SerIle: 4.64 ± 0.096
3.625SerLys: 3.625 ± 0.092
5.873SerLeu: 5.873 ± 0.121
1.617SerMet: 1.617 ± 0.054
2.496SerAsn: 2.496 ± 0.071
2.787SerPro: 2.787 ± 0.073
2.443SerGln: 2.443 ± 0.063
2.571SerArg: 2.571 ± 0.066
4.169SerSer: 4.169 ± 0.096
3.415SerThr: 3.415 ± 0.078
3.6SerVal: 3.6 ± 0.077
0.529SerTrp: 0.529 ± 0.035
2.148SerTyr: 2.148 ± 0.068
0.0SerXaa: 0.0 ± 0.0
Thr
3.549ThrAla: 3.549 ± 0.09
0.661ThrCys: 0.661 ± 0.037
2.644ThrAsp: 2.644 ± 0.071
3.241ThrGlu: 3.241 ± 0.08
1.944ThrPhe: 1.944 ± 0.06
4.428ThrGly: 4.428 ± 0.103
1.015ThrHis: 1.015 ± 0.048
4.308ThrIle: 4.308 ± 0.098
2.635ThrLys: 2.635 ± 0.077
5.078ThrLeu: 5.078 ± 0.091
1.376ThrMet: 1.376 ± 0.05
2.142ThrAsn: 2.142 ± 0.069
2.864ThrPro: 2.864 ± 0.075
1.547ThrGln: 1.547 ± 0.053
2.301ThrArg: 2.301 ± 0.059
3.511ThrSer: 3.511 ± 0.077
3.093ThrThr: 3.093 ± 0.095
3.861ThrVal: 3.861 ± 0.113
0.418ThrTrp: 0.418 ± 0.029
1.646ThrTyr: 1.646 ± 0.066
0.0ThrXaa: 0.0 ± 0.0
Val
5.021ValAla: 5.021 ± 0.11
0.915ValCys: 0.915 ± 0.049
4.687ValAsp: 4.687 ± 0.106
5.836ValGlu: 5.836 ± 0.117
2.666ValPhe: 2.666 ± 0.078
5.169ValGly: 5.169 ± 0.111
1.211ValHis: 1.211 ± 0.048
5.798ValIle: 5.798 ± 0.12
5.221ValLys: 5.221 ± 0.112
6.729ValLeu: 6.729 ± 0.12
1.949ValMet: 1.949 ± 0.067
3.093ValAsn: 3.093 ± 0.094
2.687ValPro: 2.687 ± 0.074
1.971ValGln: 1.971 ± 0.054
2.548ValArg: 2.548 ± 0.067
4.031ValSer: 4.031 ± 0.094
3.727ValThr: 3.727 ± 0.088
5.825ValVal: 5.825 ± 0.111
0.484ValTrp: 0.484 ± 0.028
2.283ValTyr: 2.283 ± 0.065
0.0ValXaa: 0.0 ± 0.0
Trp
0.507TrpAla: 0.507 ± 0.033
0.1TrpCys: 0.1 ± 0.013
0.568TrpAsp: 0.568 ± 0.033
0.554TrpGlu: 0.554 ± 0.035
0.338TrpPhe: 0.338 ± 0.023
0.57TrpGly: 0.57 ± 0.031
0.132TrpHis: 0.132 ± 0.015
0.674TrpIle: 0.674 ± 0.041
0.579TrpLys: 0.579 ± 0.033
0.7TrpLeu: 0.7 ± 0.036
0.252TrpMet: 0.252 ± 0.019
0.468TrpAsn: 0.468 ± 0.034
0.222TrpPro: 0.222 ± 0.02
0.245TrpGln: 0.245 ± 0.022
0.32TrpArg: 0.32 ± 0.029
0.465TrpSer: 0.465 ± 0.027
0.388TrpThr: 0.388 ± 0.026
0.536TrpVal: 0.536 ± 0.031
0.145TrpTrp: 0.145 ± 0.018
0.297TrpTyr: 0.297 ± 0.023
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.908TyrAla: 1.908 ± 0.056
0.473TyrCys: 0.473 ± 0.033
1.717TyrAsp: 1.717 ± 0.059
1.88TyrGlu: 1.88 ± 0.059
1.485TyrPhe: 1.485 ± 0.049
2.487TyrGly: 2.487 ± 0.076
0.895TyrHis: 0.895 ± 0.044
2.471TyrIle: 2.471 ± 0.07
1.955TyrLys: 1.955 ± 0.066
3.686TyrLeu: 3.686 ± 0.085
0.824TyrMet: 0.824 ± 0.036
1.671TyrAsn: 1.671 ± 0.06
1.705TyrPro: 1.705 ± 0.056
1.794TyrGln: 1.794 ± 0.058
1.397TyrArg: 1.397 ± 0.047
2.225TyrSer: 2.225 ± 0.078
1.66TyrThr: 1.66 ± 0.055
2.124TyrVal: 2.124 ± 0.072
0.35TyrTrp: 0.35 ± 0.027
1.313TyrTyr: 1.313 ± 0.047
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2013 proteins (559669 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski