Streptomyces vitaminophilus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5132 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0T6LWF3|A0A0T6LWF3_9ACTN S-adenosylmethionine synthase OS=Streptomyces vitaminophilus OX=76728 GN=metK PE=3 SV=1
MM1 pKa = 7.75PVNQEE6 pKa = 4.0ALEE9 pKa = 4.03VWIDD13 pKa = 3.62QEE15 pKa = 4.65LCTGDD20 pKa = 4.86GICAQYY26 pKa = 11.17APDD29 pKa = 4.08VFEE32 pKa = 6.18LDD34 pKa = 3.22IDD36 pKa = 3.59GLAYY40 pKa = 10.69VKK42 pKa = 11.17GEE44 pKa = 4.03DD45 pKa = 4.31DD46 pKa = 4.93EE47 pKa = 5.73LRR49 pKa = 11.84QLPGQTVPVPLPLLTDD65 pKa = 3.61VVEE68 pKa = 4.41SAKK71 pKa = 10.66DD72 pKa = 3.55CPGEE76 pKa = 4.36CIHH79 pKa = 6.92IRR81 pKa = 11.84RR82 pKa = 11.84LSDD85 pKa = 3.0GAEE88 pKa = 4.16VYY90 pKa = 10.88GPAAQAA96 pKa = 3.46

Molecular weight: 10.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0T6LN19|A0A0T6LN19_9ACTN Uncharacterized protein OS=Streptomyces vitaminophilus OX=76728 GN=AQ490_06135 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIIASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 11.02GRR40 pKa = 11.84GRR42 pKa = 11.84LSAA45 pKa = 4.4

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5132

0

5132

1739815

37

4989

339.0

36.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.057 ± 0.049

0.839 ± 0.009

6.117 ± 0.024

5.67 ± 0.032

2.647 ± 0.019

9.474 ± 0.029

2.396 ± 0.017

2.787 ± 0.024

1.774 ± 0.023

10.345 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.656 ± 0.014

1.776 ± 0.017

6.346 ± 0.037

2.847 ± 0.019

8.644 ± 0.041

5.0 ± 0.022

6.138 ± 0.039

8.924 ± 0.028

1.533 ± 0.015

2.029 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski