Amino acid dipepetide frequency for Streptomyces vitaminophilus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.683AlaAla: 19.683 ± 0.149	1.095AlaCys: 1.095 ± 0.027	8.032AlaAsp: 8.032 ± 0.07	8.522AlaGlu: 8.522 ± 0.099	3.326AlaPhe: 3.326 ± 0.04	12.579AlaGly: 12.579 ± 0.101	2.8AlaHis: 2.8 ± 0.036	3.004AlaIle: 3.004 ± 0.049	2.317AlaLys: 2.317 ± 0.045	14.135AlaLeu: 14.135 ± 0.114	2.284AlaMet: 2.284 ± 0.037	1.936AlaAsn: 1.936 ± 0.033	7.061AlaPro: 7.061 ± 0.082	3.608AlaGln: 3.608 ± 0.053	10.713AlaArg: 10.713 ± 0.102	5.802AlaSer: 5.802 ± 0.06	7.01AlaThr: 7.01 ± 0.069	12.403AlaVal: 12.403 ± 0.101	1.782AlaTrp: 1.782 ± 0.037	2.476AlaTyr: 2.476 ± 0.041	0.0AlaXaa: 0.0 ± 0.0
Cys	1.147CysAla: 1.147 ± 0.031	0.102CysCys: 0.102 ± 0.008	0.521CysAsp: 0.521 ± 0.018	0.39CysGlu: 0.39 ± 0.015	0.213CysPhe: 0.213 ± 0.011	1.054CysGly: 1.054 ± 0.028	0.214CysHis: 0.214 ± 0.011	0.145CysIle: 0.145 ± 0.01	0.087CysLys: 0.087 ± 0.007	0.838CysLeu: 0.838 ± 0.02	0.116CysMet: 0.116 ± 0.008	0.13CysAsn: 0.13 ± 0.01	0.523CysPro: 0.523 ± 0.017	0.203CysGln: 0.203 ± 0.011	0.691CysArg: 0.691 ± 0.026	0.473CysSer: 0.473 ± 0.02	0.526CysThr: 0.526 ± 0.02	0.726CysVal: 0.726 ± 0.022	0.132CysTrp: 0.132 ± 0.009	0.16CysTyr: 0.16 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	7.484AspAla: 7.484 ± 0.069	0.436AspCys: 0.436 ± 0.019	3.722AspAsp: 3.722 ± 0.057	3.78AspGlu: 3.78 ± 0.055	1.561AspPhe: 1.561 ± 0.035	6.46AspGly: 6.46 ± 0.07	1.571AspHis: 1.571 ± 0.028	1.701AspIle: 1.701 ± 0.039	0.966AspLys: 0.966 ± 0.029	6.633AspLeu: 6.633 ± 0.066	0.783AspMet: 0.783 ± 0.02	0.974AspAsn: 0.974 ± 0.028	4.902AspPro: 4.902 ± 0.063	1.867AspGln: 1.867 ± 0.035	5.341AspArg: 5.341 ± 0.062	2.472AspSer: 2.472 ± 0.04	3.338AspThr: 3.338 ± 0.046	4.992AspVal: 4.992 ± 0.057	1.05AspTrp: 1.05 ± 0.025	1.141AspTyr: 1.141 ± 0.029	0.0AspXaa: 0.0 ± 0.0
Glu	6.942GluAla: 6.942 ± 0.078	0.374GluCys: 0.374 ± 0.014	2.872GluAsp: 2.872 ± 0.045	3.52GluGlu: 3.52 ± 0.056	1.461GluPhe: 1.461 ± 0.03	4.237GluGly: 4.237 ± 0.054	1.605GluHis: 1.605 ± 0.03	1.969GluIle: 1.969 ± 0.039	1.135GluLys: 1.135 ± 0.028	7.022GluLeu: 7.022 ± 0.082	0.878GluMet: 0.878 ± 0.024	0.952GluAsn: 0.952 ± 0.024	3.647GluPro: 3.647 ± 0.053	2.305GluGln: 2.305 ± 0.039	5.741GluArg: 5.741 ± 0.073	2.369GluSer: 2.369 ± 0.039	2.555GluThr: 2.555 ± 0.037	5.16GluVal: 5.16 ± 0.064	0.777GluTrp: 0.777 ± 0.023	1.175GluTyr: 1.175 ± 0.027	0.0GluXaa: 0.0 ± 0.0
Phe	3.412PheAla: 3.412 ± 0.047	0.274PheCys: 0.274 ± 0.013	2.015PheAsp: 2.015 ± 0.034	1.344PheGlu: 1.344 ± 0.029	0.777PhePhe: 0.777 ± 0.023	2.925PheGly: 2.925 ± 0.04	0.674PheHis: 0.674 ± 0.018	0.558PheIle: 0.558 ± 0.02	0.4PheLys: 0.4 ± 0.015	2.534PheLeu: 2.534 ± 0.041	0.367PheMet: 0.367 ± 0.016	0.54PheAsn: 0.54 ± 0.018	1.404PhePro: 1.404 ± 0.031	0.717PheGln: 0.717 ± 0.019	1.954PheArg: 1.954 ± 0.031	1.46PheSer: 1.46 ± 0.03	1.991PheThr: 1.991 ± 0.037	2.177PheVal: 2.177 ± 0.037	0.407PheTrp: 0.407 ± 0.015	0.541PheTyr: 0.541 ± 0.017	0.0PheXaa: 0.0 ± 0.0
Gly	10.976GlyAla: 10.976 ± 0.089	0.921GlyCys: 0.921 ± 0.025	5.415GlyAsp: 5.415 ± 0.058	5.233GlyGlu: 5.233 ± 0.06	2.7GlyPhe: 2.7 ± 0.038	8.752GlyGly: 8.752 ± 0.084	2.397GlyHis: 2.397 ± 0.045	2.942GlyIle: 2.942 ± 0.044	2.08GlyLys: 2.08 ± 0.043	9.179GlyLeu: 9.179 ± 0.08	1.954GlyMet: 1.954 ± 0.03	1.711GlyAsn: 1.711 ± 0.038	5.654GlyPro: 5.654 ± 0.069	2.86GlyGln: 2.86 ± 0.048	8.232GlyArg: 8.232 ± 0.068	5.171GlySer: 5.171 ± 0.061	6.585GlyThr: 6.585 ± 0.068	7.911GlyVal: 7.911 ± 0.07	1.749GlyTrp: 1.749 ± 0.036	2.324GlyTyr: 2.324 ± 0.041	0.0GlyXaa: 0.0 ± 0.0
His	2.664HisAla: 2.664 ± 0.044	0.24HisCys: 0.24 ± 0.011	1.405HisAsp: 1.405 ± 0.028	1.137HisGlu: 1.137 ± 0.027	0.616HisPhe: 0.616 ± 0.018	2.538HisGly: 2.538 ± 0.044	0.795HisHis: 0.795 ± 0.024	0.56HisIle: 0.56 ± 0.019	0.299HisLys: 0.299 ± 0.014	2.746HisLeu: 2.746 ± 0.044	0.312HisMet: 0.312 ± 0.014	0.384HisAsn: 0.384 ± 0.016	1.968HisPro: 1.968 ± 0.037	0.826HisGln: 0.826 ± 0.021	2.486HisArg: 2.486 ± 0.042	1.008HisSer: 1.008 ± 0.025	1.381HisThr: 1.381 ± 0.031	1.727HisVal: 1.727 ± 0.03	0.413HisTrp: 0.413 ± 0.014	0.458HisTyr: 0.458 ± 0.015	0.0HisXaa: 0.0 ± 0.0
Ile	3.997IleAla: 3.997 ± 0.05	0.258IleCys: 0.258 ± 0.012	2.193IleAsp: 2.193 ± 0.037	1.724IleGlu: 1.724 ± 0.037	0.609IlePhe: 0.609 ± 0.019	2.995IleGly: 2.995 ± 0.047	0.582IleHis: 0.582 ± 0.019	0.741IleIle: 0.741 ± 0.026	0.668IleLys: 0.668 ± 0.023	1.929IleLeu: 1.929 ± 0.038	0.381IleMet: 0.381 ± 0.016	0.697IleAsn: 0.697 ± 0.026	1.55IlePro: 1.55 ± 0.033	0.647IleGln: 0.647 ± 0.021	2.035IleArg: 2.035 ± 0.035	1.613IleSer: 1.613 ± 0.035	2.108IleThr: 2.108 ± 0.046	2.314IleVal: 2.314 ± 0.041	0.344IleTrp: 0.344 ± 0.014	0.485IleTyr: 0.485 ± 0.018	0.0IleXaa: 0.0 ± 0.0
Lys	2.273LysAla: 2.273 ± 0.043	0.098LysCys: 0.098 ± 0.008	1.1LysAsp: 1.1 ± 0.027	1.048LysGlu: 1.048 ± 0.03	0.356LysPhe: 0.356 ± 0.016	1.505LysGly: 1.505 ± 0.035	0.39LysHis: 0.39 ± 0.015	0.735LysIle: 0.735 ± 0.022	0.641LysLys: 0.641 ± 0.025	1.692LysLeu: 1.692 ± 0.041	0.308LysMet: 0.308 ± 0.014	0.446LysAsn: 0.446 ± 0.018	1.099LysPro: 1.099 ± 0.028	0.579LysGln: 0.579 ± 0.019	1.244LysArg: 1.244 ± 0.028	0.924LysSer: 0.924 ± 0.025	1.037LysThr: 1.037 ± 0.029	1.683LysVal: 1.683 ± 0.036	0.193LysTrp: 0.193 ± 0.013	0.393LysTyr: 0.393 ± 0.019	0.0LysXaa: 0.0 ± 0.0
Leu	14.89LeuAla: 14.89 ± 0.129	0.873LeuCys: 0.873 ± 0.025	6.904LeuAsp: 6.904 ± 0.076	4.958LeuGlu: 4.958 ± 0.054	2.543LeuPhe: 2.543 ± 0.046	9.293LeuGly: 9.293 ± 0.085	2.367LeuHis: 2.367 ± 0.045	2.757LeuIle: 2.757 ± 0.048	1.638LeuLys: 1.638 ± 0.039	11.421LeuLeu: 11.421 ± 0.117	1.456LeuMet: 1.456 ± 0.03	1.704LeuAsn: 1.704 ± 0.034	6.566LeuPro: 6.566 ± 0.079	2.037LeuGln: 2.037 ± 0.036	9.395LeuArg: 9.395 ± 0.085	5.279LeuSer: 5.279 ± 0.063	6.889LeuThr: 6.889 ± 0.066	9.48LeuVal: 9.48 ± 0.093	1.246LeuTrp: 1.246 ± 0.031	1.759LeuTyr: 1.759 ± 0.031	0.0LeuXaa: 0.0 ± 0.0
Met	2.194MetAla: 2.194 ± 0.031	0.122MetCys: 0.122 ± 0.008	0.886MetAsp: 0.886 ± 0.025	0.754MetGlu: 0.754 ± 0.021	0.445MetPhe: 0.445 ± 0.016	1.293MetGly: 1.293 ± 0.03	0.344MetHis: 0.344 ± 0.013	0.555MetIle: 0.555 ± 0.021	0.318MetLys: 0.318 ± 0.015	1.625MetLeu: 1.625 ± 0.028	0.253MetMet: 0.253 ± 0.013	0.39MetAsn: 0.39 ± 0.014	1.01MetPro: 1.01 ± 0.024	0.396MetGln: 0.396 ± 0.014	1.45MetArg: 1.45 ± 0.033	1.225MetSer: 1.225 ± 0.027	1.419MetThr: 1.419 ± 0.029	1.37MetVal: 1.37 ± 0.033	0.205MetTrp: 0.205 ± 0.011	0.301MetTyr: 0.301 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.112AsnAla: 2.112 ± 0.035	0.169AsnCys: 0.169 ± 0.01	1.057AsnAsp: 1.057 ± 0.029	0.92AsnGlu: 0.92 ± 0.024	0.449AsnPhe: 0.449 ± 0.017	1.912AsnGly: 1.912 ± 0.04	0.415AsnHis: 0.415 ± 0.014	0.535AsnIle: 0.535 ± 0.017	0.355AsnLys: 0.355 ± 0.016	1.763AsnLeu: 1.763 ± 0.036	0.274AsnMet: 0.274 ± 0.013	0.406AsnAsn: 0.406 ± 0.021	1.351AsnPro: 1.351 ± 0.03	0.556AsnGln: 0.556 ± 0.017	1.325AsnArg: 1.325 ± 0.028	0.901AsnSer: 0.901 ± 0.029	1.101AsnThr: 1.101 ± 0.029	1.45AsnVal: 1.45 ± 0.029	0.28AsnTrp: 0.28 ± 0.013	0.428AsnTyr: 0.428 ± 0.018	0.0AsnXaa: 0.0 ± 0.0
Pro	8.331ProAla: 8.331 ± 0.077	0.382ProCys: 0.382 ± 0.015	4.848ProAsp: 4.848 ± 0.063	4.367ProGlu: 4.367 ± 0.053	1.558ProPhe: 1.558 ± 0.031	7.332ProGly: 7.332 ± 0.079	1.534ProHis: 1.534 ± 0.03	1.22ProIle: 1.22 ± 0.03	0.964ProLys: 0.964 ± 0.026	5.325ProLeu: 5.325 ± 0.066	0.973ProMet: 0.973 ± 0.025	0.982ProAsn: 0.982 ± 0.03	4.103ProPro: 4.103 ± 0.072	1.855ProGln: 1.855 ± 0.041	4.701ProArg: 4.701 ± 0.051	3.318ProSer: 3.318 ± 0.05	3.588ProThr: 3.588 ± 0.05	5.817ProVal: 5.817 ± 0.062	0.987ProTrp: 0.987 ± 0.024	1.27ProTyr: 1.27 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	3.696GlnAla: 3.696 ± 0.057	0.183GlnCys: 0.183 ± 0.01	1.668GlnAsp: 1.668 ± 0.032	1.602GlnGlu: 1.602 ± 0.034	0.698GlnPhe: 0.698 ± 0.022	2.383GlnGly: 2.383 ± 0.037	0.708GlnHis: 0.708 ± 0.021	0.971GlnIle: 0.971 ± 0.024	0.46GlnLys: 0.46 ± 0.02	2.785GlnLeu: 2.785 ± 0.046	0.508GlnMet: 0.508 ± 0.018	0.474GlnAsn: 0.474 ± 0.017	1.916GlnPro: 1.916 ± 0.046	1.279GlnGln: 1.279 ± 0.033	2.785GlnArg: 2.785 ± 0.045	1.152GlnSer: 1.152 ± 0.033	1.292GlnThr: 1.292 ± 0.032	2.862GlnVal: 2.862 ± 0.042	0.476GlnTrp: 0.476 ± 0.019	0.579GlnTyr: 0.579 ± 0.019	0.0GlnXaa: 0.0 ± 0.0
Arg	10.636ArgAla: 10.636 ± 0.098	0.746ArgCys: 0.746 ± 0.023	4.79ArgAsp: 4.79 ± 0.057	4.941ArgGlu: 4.941 ± 0.053	2.365ArgPhe: 2.365 ± 0.04	6.721ArgGly: 6.721 ± 0.073	2.262ArgHis: 2.262 ± 0.037	2.934ArgIle: 2.934 ± 0.044	1.434ArgLys: 1.434 ± 0.033	9.401ArgLeu: 9.401 ± 0.099	1.843ArgMet: 1.843 ± 0.033	1.527ArgAsn: 1.527 ± 0.034	5.367ArgPro: 5.367 ± 0.066	2.695ArgGln: 2.695 ± 0.048	8.661ArgArg: 8.661 ± 0.095	4.298ArgSer: 4.298 ± 0.051	5.355ArgThr: 5.355 ± 0.062	6.955ArgVal: 6.955 ± 0.064	1.538ArgTrp: 1.538 ± 0.032	1.971ArgTyr: 1.971 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	6.224SerAla: 6.224 ± 0.064	0.418SerCys: 0.418 ± 0.016	2.746SerAsp: 2.746 ± 0.047	2.38SerGlu: 2.38 ± 0.039	1.577SerPhe: 1.577 ± 0.036	5.878SerGly: 5.878 ± 0.069	1.075SerHis: 1.075 ± 0.024	1.27SerIle: 1.27 ± 0.026	0.859SerLys: 0.859 ± 0.026	4.683SerLeu: 4.683 ± 0.052	0.977SerMet: 0.977 ± 0.024	0.896SerAsn: 0.896 ± 0.026	3.352SerPro: 3.352 ± 0.055	1.255SerGln: 1.255 ± 0.029	3.981SerArg: 3.981 ± 0.051	2.892SerSer: 2.892 ± 0.056	3.109SerThr: 3.109 ± 0.054	4.372SerVal: 4.372 ± 0.043	0.889SerTrp: 0.889 ± 0.025	1.166SerTyr: 1.166 ± 0.023	0.0SerXaa: 0.0 ± 0.0
Thr	8.58ThrAla: 8.58 ± 0.076	0.475ThrCys: 0.475 ± 0.016	3.551ThrAsp: 3.551 ± 0.047	3.23ThrGlu: 3.23 ± 0.045	1.672ThrPhe: 1.672 ± 0.034	6.778ThrGly: 6.778 ± 0.074	1.282ThrHis: 1.282 ± 0.03	1.617ThrIle: 1.617 ± 0.038	1.037ThrLys: 1.037 ± 0.035	5.638ThrLeu: 5.638 ± 0.06	1.012ThrMet: 1.012 ± 0.028	1.022ThrAsn: 1.022 ± 0.027	4.142ThrPro: 4.142 ± 0.043	1.319ThrGln: 1.319 ± 0.028	4.228ThrArg: 4.228 ± 0.05	3.235ThrSer: 3.235 ± 0.047	3.983ThrThr: 3.983 ± 0.076	6.354ThrVal: 6.354 ± 0.065	0.963ThrTrp: 0.963 ± 0.025	1.266ThrTyr: 1.266 ± 0.032	0.0ThrXaa: 0.0 ± 0.0
Val	11.135ValAla: 11.135 ± 0.092	0.852ValCys: 0.852 ± 0.022	5.184ValAsp: 5.184 ± 0.059	5.09ValGlu: 5.09 ± 0.054	2.426ValPhe: 2.426 ± 0.039	7.046ValGly: 7.046 ± 0.069	2.146ValHis: 2.146 ± 0.036	2.764ValIle: 2.764 ± 0.046	1.489ValLys: 1.489 ± 0.035	10.064ValLeu: 10.064 ± 0.088	1.34ValMet: 1.34 ± 0.028	1.789ValAsn: 1.789 ± 0.04	5.838ValPro: 5.838 ± 0.066	2.1ValGln: 2.1 ± 0.038	7.957ValArg: 7.957 ± 0.078	4.508ValSer: 4.508 ± 0.056	5.937ValThr: 5.937 ± 0.067	8.805ValVal: 8.805 ± 0.086	1.19ValTrp: 1.19 ± 0.028	1.576ValTyr: 1.576 ± 0.032	0.0ValXaa: 0.0 ± 0.0
Trp	1.609TrpAla: 1.609 ± 0.032	0.171TrpCys: 0.171 ± 0.01	0.92TrpAsp: 0.92 ± 0.027	0.744TrpGlu: 0.744 ± 0.022	0.496TrpPhe: 0.496 ± 0.017	1.05TrpGly: 1.05 ± 0.025	0.374TrpHis: 0.374 ± 0.015	0.489TrpIle: 0.489 ± 0.018	0.31TrpLys: 0.31 ± 0.015	1.782TrpLeu: 1.782 ± 0.037	0.3TrpMet: 0.3 ± 0.014	0.439TrpAsn: 0.439 ± 0.016	0.833TrpPro: 0.833 ± 0.024	0.6TrpGln: 0.6 ± 0.021	1.448TrpArg: 1.448 ± 0.029	0.916TrpSer: 0.916 ± 0.025	1.044TrpThr: 1.044 ± 0.029	1.067TrpVal: 1.067 ± 0.031	0.384TrpTrp: 0.384 ± 0.016	0.356TrpTyr: 0.356 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.582TyrAla: 2.582 ± 0.046	0.204TyrCys: 0.204 ± 0.01	1.348TyrAsp: 1.348 ± 0.032	1.01TyrGlu: 1.01 ± 0.024	0.621TyrPhe: 0.621 ± 0.021	2.015TyrGly: 2.015 ± 0.038	0.429TyrHis: 0.429 ± 0.015	0.402TyrIle: 0.402 ± 0.018	0.287TyrLys: 0.287 ± 0.013	2.26TyrLeu: 2.26 ± 0.038	0.237TyrMet: 0.237 ± 0.013	0.362TyrAsn: 0.362 ± 0.017	1.169TyrPro: 1.169 ± 0.026	0.771TyrGln: 0.771 ± 0.024	2.073TyrArg: 2.073 ± 0.035	0.98TyrSer: 0.98 ± 0.026	1.136TyrThr: 1.136 ± 0.033	1.611TyrVal: 1.611 ± 0.032	0.326TyrTrp: 0.326 ± 0.014	0.469TyrTyr: 0.469 ± 0.019	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5132 proteins (1739815 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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