Haemophilus paracuniculus
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1927 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1T0AW36|A0A1T0AW36_9PAST DNA topoisomerase OS=Haemophilus paracuniculus OX=734 GN=B0187_00760 PE=3 SV=1
MM1 pKa = 7.73 PNWCEE6 pKa = 3.82 NKK8 pKa = 10.28 LDD10 pKa = 4.76 FYY12 pKa = 11.44 CPTDD16 pKa = 3.62 QLDD19 pKa = 3.6 NLKK22 pKa = 10.76 NKK24 pKa = 9.92 LFSKK28 pKa = 9.67 TDD30 pKa = 3.45 EE31 pKa = 4.79 GNLCLDD37 pKa = 3.95 FTKK40 pKa = 10.56 LKK42 pKa = 10.2 PMPVEE47 pKa = 4.42 LDD49 pKa = 3.57 IEE51 pKa = 4.46 CSSMGTKK58 pKa = 9.67 SRR60 pKa = 11.84 QLLEE64 pKa = 3.86 EE65 pKa = 3.91 FQYY68 pKa = 11.48 QLFSLEE74 pKa = 4.4 LMEE77 pKa = 5.61 KK78 pKa = 9.84 YY79 pKa = 10.51 LPRR82 pKa = 11.84 SQVNRR87 pKa = 11.84 IYY89 pKa = 10.62 TLATQQMWSLNQFVEE104 pKa = 4.36 WLQANPSEE112 pKa = 4.14 QKK114 pKa = 10.94 VLGVDD119 pKa = 3.81 LTLGQQYY126 pKa = 10.46 IDD128 pKa = 3.73 NLAKK132 pKa = 10.82 YY133 pKa = 9.83 GVEE136 pKa = 3.72 TWYY139 pKa = 10.27 DD140 pKa = 2.85 WHH142 pKa = 6.22 CQQWGCKK149 pKa = 7.05 WNATTYY155 pKa = 10.68 DD156 pKa = 3.12 ITEE159 pKa = 4.19 SDD161 pKa = 3.59 GHH163 pKa = 5.06 ITCYY167 pKa = 10.5 FDD169 pKa = 4.67 TPWSPPRR176 pKa = 11.84 EE177 pKa = 4.1 WFEE180 pKa = 4.2 TLCEE184 pKa = 4.07 TFPTIDD190 pKa = 3.36 FTLSFFEE197 pKa = 4.55 PGWWFAGDD205 pKa = 4.85 FIADD209 pKa = 3.64 MSGSFYY215 pKa = 10.89 EE216 pKa = 4.22 NHH218 pKa = 6.78 LCDD221 pKa = 5.92 DD222 pKa = 4.81 EE223 pKa = 5.28 IKK225 pKa = 10.06 TFAKK229 pKa = 10.47 DD230 pKa = 3.36 VFGEE234 pKa = 4.16 EE235 pKa = 4.35 FDD237 pKa = 6.02 DD238 pKa = 5.6 EE239 pKa = 4.73 EE240 pKa = 6.56 DD241 pKa = 4.16 DD242 pKa = 5.06 NEE244 pKa = 4.96 GEE246 pKa = 4.11
Molecular weight: 29.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.803
IPC2_protein 4.05
IPC_protein 4.024
Toseland 3.834
ProMoST 4.139
Dawson 3.999
Bjellqvist 4.164
Wikipedia 3.884
Rodwell 3.859
Grimsley 3.745
Solomon 3.986
Lehninger 3.948
Nozaki 4.101
DTASelect 4.291
Thurlkill 3.859
EMBOSS 3.897
Sillero 4.139
Patrickios 1.291
IPC_peptide 3.986
IPC2_peptide 4.126
IPC2.peptide.svr19 4.036
Protein with the highest isoelectric point:
>tr|A0A1T0AVF5|A0A1T0AVF5_9PAST Uncharacterized protein OS=Haemophilus paracuniculus OX=734 GN=B0187_00740 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.49 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.91 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.15 GRR39 pKa = 11.84 KK40 pKa = 8.87 SLSAA44 pKa = 3.86
Molecular weight: 5.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1927
0
1927
593209
30
1816
307.8
34.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.915 ± 0.087
0.995 ± 0.021
4.897 ± 0.04
6.432 ± 0.055
4.467 ± 0.044
6.709 ± 0.056
1.861 ± 0.024
6.59 ± 0.05
6.135 ± 0.055
10.528 ± 0.074
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.304 ± 0.031
4.772 ± 0.059
3.913 ± 0.033
5.13 ± 0.056
4.529 ± 0.047
5.77 ± 0.052
5.133 ± 0.042
6.731 ± 0.049
1.138 ± 0.022
3.052 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here