Desulfobulbus oralis
Average proteome isoelectric point is 6.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2278 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2L1GMG9|A0A2L1GMG9_9DELT Type I-E CRISPR-associated protein Cas6/Cse3/CasE OS=Desulfobulbus oralis OX=1986146 GN=CAY53_04850 PE=4 SV=1
MM1 pKa = 7.7 KK2 pKa = 10.21 KK3 pKa = 9.85 YY4 pKa = 10.35 QCDD7 pKa = 3.52 VCGYY11 pKa = 10.01 VYY13 pKa = 10.68 DD14 pKa = 4.46 PAVGDD19 pKa = 3.99 PDD21 pKa = 4.71 GGIAPGTAFEE31 pKa = 5.99 DD32 pKa = 4.17 IPADD36 pKa = 3.67 WVCPVCGAAKK46 pKa = 10.39 DD47 pKa = 3.97 DD48 pKa = 4.25 FSPVEE53 pKa = 4.07 DD54 pKa = 3.62
Molecular weight: 5.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.866
IPC2_protein 3.617
IPC_protein 3.554
Toseland 3.338
ProMoST 3.681
Dawson 3.592
Bjellqvist 3.846
Wikipedia 3.605
Rodwell 3.401
Grimsley 3.261
Solomon 3.541
Lehninger 3.503
Nozaki 3.732
DTASelect 3.999
Thurlkill 3.439
EMBOSS 3.605
Sillero 3.694
Patrickios 0.121
IPC_peptide 3.541
IPC2_peptide 3.656
IPC2.peptide.svr19 3.747
Protein with the highest isoelectric point:
>tr|A0A2L1GRL1|A0A2L1GRL1_9DELT Aspartate racemase OS=Desulfobulbus oralis OX=1986146 GN=CAY53_07770 PE=3 SV=1
MM1 pKa = 7.56 SKK3 pKa = 10.81 GKK5 pKa = 8.71 GTFQPGRR12 pKa = 11.84 TSRR15 pKa = 11.84 SRR17 pKa = 11.84 THH19 pKa = 6.2 GFLVRR24 pKa = 11.84 MRR26 pKa = 11.84 TRR28 pKa = 11.84 SGQSVIRR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 AKK40 pKa = 10.04 GRR42 pKa = 11.84 KK43 pKa = 8.32 QLAPP47 pKa = 3.78
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.647
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.51
Sillero 12.998
Patrickios 12.369
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.185
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2278
0
2278
767338
35
2969
336.8
37.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.33 ± 0.081
1.515 ± 0.022
4.963 ± 0.042
6.018 ± 0.045
3.92 ± 0.036
8.195 ± 0.061
2.21 ± 0.022
4.971 ± 0.051
3.784 ± 0.044
11.362 ± 0.076
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.544 ± 0.022
2.843 ± 0.033
4.944 ± 0.038
3.953 ± 0.033
7.131 ± 0.055
5.375 ± 0.047
4.558 ± 0.032
6.572 ± 0.041
1.209 ± 0.021
2.602 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here