Nocardioides currus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4242 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R7YXU4|A0A2R7YXU4_9ACTN Uridylate kinase OS=Nocardioides currus OX=2133958 GN=pyrH PE=3 SV=1
MM1 pKa = 7.13TVDD4 pKa = 4.37AVDD7 pKa = 4.17DD8 pKa = 3.72QAVANDD14 pKa = 3.96DD15 pKa = 3.78EE16 pKa = 4.56LTIEE20 pKa = 4.23EE21 pKa = 4.84DD22 pKa = 3.4ADD24 pKa = 3.66ATEE27 pKa = 4.32VDD29 pKa = 4.05VLANDD34 pKa = 3.81TDD36 pKa = 4.15VEE38 pKa = 4.23GDD40 pKa = 4.08PIEE43 pKa = 4.23VTAVGDD49 pKa = 4.03AEE51 pKa = 4.86HH52 pKa = 6.35GTVTLVDD59 pKa = 3.77GVVTYY64 pKa = 9.23TPNANYY70 pKa = 10.65NGTDD74 pKa = 3.07SFTYY78 pKa = 9.81TVTDD82 pKa = 3.89GATATVAVTVNAVDD96 pKa = 4.49DD97 pKa = 4.5KK98 pKa = 11.36PVAHH102 pKa = 7.44DD103 pKa = 4.4DD104 pKa = 3.86EE105 pKa = 4.95VTVTEE110 pKa = 4.99DD111 pKa = 3.26DD112 pKa = 3.65PAAVVDD118 pKa = 4.23VLANDD123 pKa = 3.7TDD125 pKa = 3.8IDD127 pKa = 4.03GGPKK131 pKa = 9.61HH132 pKa = 5.81VASVGAAAHH141 pKa = 5.38GTVTLVDD148 pKa = 3.82GVVSYY153 pKa = 10.98RR154 pKa = 11.84PNANHH159 pKa = 7.02AGSDD163 pKa = 3.53SFTYY167 pKa = 9.11TVNGGSSATVDD178 pKa = 3.23ITVSAVDD185 pKa = 3.85DD186 pKa = 4.51APVAVADD193 pKa = 4.01SAAVAEE199 pKa = 4.82DD200 pKa = 3.85ASATAINVLANDD212 pKa = 3.84TDD214 pKa = 3.81IDD216 pKa = 4.13GGPKK220 pKa = 10.3AIMSATNPAHH230 pKa = 5.18GTVVITGGGSGLTYY244 pKa = 10.45RR245 pKa = 11.84PAANYY250 pKa = 10.19NGADD254 pKa = 3.33SFTYY258 pKa = 9.39TLNGGSTATVSITVTAVDD276 pKa = 4.16DD277 pKa = 4.42APVAVNDD284 pKa = 4.39AYY286 pKa = 9.8TVSEE290 pKa = 4.61DD291 pKa = 3.93SNATTLPVLANDD303 pKa = 4.05ADD305 pKa = 3.98IDD307 pKa = 4.16GGPKK311 pKa = 9.97TITAVTQPANGTVTITGGGTGLTYY335 pKa = 10.66KK336 pKa = 10.56PATNYY341 pKa = 7.41FTPLNTPSTFTYY353 pKa = 9.95TLDD356 pKa = 3.83GGSTATVSMTVSPVADD372 pKa = 3.39NGASNNPTIEE382 pKa = 4.09VGAEE386 pKa = 3.74TTVYY390 pKa = 10.52KK391 pKa = 10.53IGTAPVVIAPNLALTHH407 pKa = 6.75PDD409 pKa = 2.8WPSFSGATVRR419 pKa = 11.84IPGTAQAGDD428 pKa = 3.42VIGFVNQNGIVGSYY442 pKa = 11.34SNGTLTLTGVASPAAYY458 pKa = 9.37QAALRR463 pKa = 11.84TVTFKK468 pKa = 10.58ATSTPEE474 pKa = 3.57TFPIVFTVTDD484 pKa = 4.05GATPARR490 pKa = 11.84SATDD494 pKa = 3.29NRR496 pKa = 11.84SVQITSS502 pKa = 3.02

Molecular weight: 50.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R7YT29|A0A2R7YT29_9ACTN Protocatechuate 3 4-dioxygenase subunit alpha OS=Nocardioides currus OX=2133958 GN=pcaG PE=3 SV=1
MM1 pKa = 7.64RR2 pKa = 11.84CPRR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84PRR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84PRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84PRR18 pKa = 11.84STRR21 pKa = 11.84PRR23 pKa = 11.84ARR25 pKa = 11.84PRR27 pKa = 11.84SRR29 pKa = 11.84PTRR32 pKa = 11.84PSRR35 pKa = 11.84ASRR38 pKa = 11.84SPRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84SRR45 pKa = 11.84PSPTQPLRR53 pKa = 11.84PPRR56 pKa = 11.84GRR58 pKa = 11.84RR59 pKa = 11.84RR60 pKa = 11.84PTRR63 pKa = 11.84AIPFPEE69 pKa = 3.76TRR71 pKa = 11.84VFLGPRR77 pKa = 3.37

Molecular weight: 9.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4242

0

4242

1384144

33

4116

326.3

34.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.943 ± 0.049

0.709 ± 0.009

6.763 ± 0.036

5.652 ± 0.041

2.839 ± 0.022

9.1 ± 0.033

2.206 ± 0.021

3.55 ± 0.025

1.971 ± 0.03

10.126 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.886 ± 0.016

1.784 ± 0.025

5.509 ± 0.023

2.679 ± 0.019

7.462 ± 0.039

5.521 ± 0.029

6.359 ± 0.047

9.477 ± 0.038

1.574 ± 0.018

1.888 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski