Klebsiella phage kpssk3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Przondovirus; Klebsiella virus kpssk3

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 42 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G8F3N5|A0A3G8F3N5_9CAUD Uncharacterized protein OS=Klebsiella phage kpssk3 OX=2488949 GN=kpssk3_011 PE=4 SV=1
MM1 pKa = 7.37EE2 pKa = 5.49RR3 pKa = 11.84NANAYY8 pKa = 9.17YY9 pKa = 10.68DD10 pKa = 4.28LVAATVKK17 pKa = 10.52LFNEE21 pKa = 5.2RR22 pKa = 11.84IQYY25 pKa = 10.61DD26 pKa = 4.24EE27 pKa = 4.38LTEE30 pKa = 4.22NDD32 pKa = 3.68DD33 pKa = 3.43WSDD36 pKa = 3.46ALHH39 pKa = 6.13EE40 pKa = 4.49VVDD43 pKa = 4.59GQVPHH48 pKa = 6.66YY49 pKa = 9.14YY50 pKa = 10.31SEE52 pKa = 4.24IFTVMAADD60 pKa = 5.69GICHH64 pKa = 6.35EE65 pKa = 5.16FDD67 pKa = 4.68DD68 pKa = 5.46SGLIPDD74 pKa = 4.27TKK76 pKa = 10.52DD77 pKa = 3.0VSRR80 pKa = 11.84ICQARR85 pKa = 11.84IYY87 pKa = 9.16EE88 pKa = 4.07ALYY91 pKa = 11.03NDD93 pKa = 3.92VLNDD97 pKa = 3.77SGIVWYY103 pKa = 10.26EE104 pKa = 3.7EE105 pKa = 4.8DD106 pKa = 5.92EE107 pKa = 4.34EE108 pKa = 5.81DD109 pKa = 5.67CDD111 pKa = 4.98DD112 pKa = 3.94

Molecular weight: 12.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G8F356|A0A3G8F356_9CAUD Uncharacterized protein OS=Klebsiella phage kpssk3 OX=2488949 GN=kpssk3_003 PE=4 SV=1
MM1 pKa = 7.54SLTITIRR8 pKa = 11.84SNTMYY13 pKa = 10.74QNTINFEE20 pKa = 4.43RR21 pKa = 11.84NRR23 pKa = 11.84EE24 pKa = 3.96RR25 pKa = 11.84QQTEE29 pKa = 4.05GYY31 pKa = 8.84IPKK34 pKa = 10.01GRR36 pKa = 11.84KK37 pKa = 8.66LNKK40 pKa = 6.36TQRR43 pKa = 11.84GGGVKK48 pKa = 10.49GSFRR52 pKa = 11.84SAKK55 pKa = 9.81GDD57 pKa = 3.61SIVNQEE63 pKa = 4.28KK64 pKa = 10.69YY65 pKa = 10.33FVGAA69 pKa = 4.14

Molecular weight: 7.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

42

0

42

11777

39

1321

280.4

31.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.06 ± 0.482

0.951 ± 0.169

6.436 ± 0.175

6.929 ± 0.455

3.626 ± 0.168

7.965 ± 0.363

1.749 ± 0.227

4.806 ± 0.144

6.649 ± 0.366

8.211 ± 0.306

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.632 ± 0.213

4.254 ± 0.263

3.753 ± 0.156

4.237 ± 0.314

5.485 ± 0.244

5.799 ± 0.357

5.536 ± 0.298

6.954 ± 0.407

1.418 ± 0.197

3.549 ± 0.234
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski