Gordonia phage Clark

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y6EIY3|A0A4Y6EIY3_9CAUD DNA methylase OS=Gordonia phage Clark OX=2588133 GN=58 PE=4 SV=1
MM1 pKa = 7.21SHH3 pKa = 7.02FAFHH7 pKa = 7.43AEE9 pKa = 3.88PTPVEE14 pKa = 4.31TRR16 pKa = 11.84HH17 pKa = 5.7GDD19 pKa = 3.71CYY21 pKa = 11.27VGTGEE26 pKa = 4.44SADD29 pKa = 3.67GVEE32 pKa = 5.05SIAIVTPGDD41 pKa = 3.92DD42 pKa = 3.55STDD45 pKa = 3.18LHH47 pKa = 7.34LSADD51 pKa = 3.57QAFVLLAEE59 pKa = 4.83LVTAIAVLVGRR70 pKa = 11.84EE71 pKa = 4.03GAPLL75 pKa = 3.68

Molecular weight: 7.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y6EH14|A0A4Y6EH14_9CAUD Uncharacterized protein OS=Gordonia phage Clark OX=2588133 GN=77 PE=4 SV=1
MM1 pKa = 6.16TTLRR5 pKa = 11.84RR6 pKa = 11.84CTRR9 pKa = 11.84PGCEE13 pKa = 3.43HH14 pKa = 7.11RR15 pKa = 11.84PRR17 pKa = 11.84SRR19 pKa = 11.84GLCHH23 pKa = 5.62THH25 pKa = 6.78YY26 pKa = 9.14MDD28 pKa = 6.13HH29 pKa = 6.62ITRR32 pKa = 11.84QKK34 pKa = 11.18AYY36 pKa = 10.13GRR38 pKa = 11.84WEE40 pKa = 3.89SSLIDD45 pKa = 3.68AEE47 pKa = 4.29PTRR50 pKa = 11.84QHH52 pKa = 4.68VQRR55 pKa = 11.84LRR57 pKa = 11.84AAGVGTRR64 pKa = 11.84KK65 pKa = 9.59ISEE68 pKa = 4.23TAGVTRR74 pKa = 11.84SVLQSLIHH82 pKa = 5.91GKK84 pKa = 7.21TRR86 pKa = 11.84NGHH89 pKa = 6.0RR90 pKa = 11.84EE91 pKa = 4.03PPSKK95 pKa = 9.88TIAKK99 pKa = 9.91RR100 pKa = 11.84IADD103 pKa = 4.63KK104 pKa = 10.77ILAVPVPAQPLHH116 pKa = 6.35NGDD119 pKa = 3.52VPALGTRR126 pKa = 11.84RR127 pKa = 11.84RR128 pKa = 11.84LQALIAHH135 pKa = 7.62GYY137 pKa = 7.86SQAEE141 pKa = 3.93INRR144 pKa = 11.84RR145 pKa = 11.84FGLAADD151 pKa = 4.22NLTEE155 pKa = 5.09LIRR158 pKa = 11.84GEE160 pKa = 4.17RR161 pKa = 11.84NYY163 pKa = 10.53VDD165 pKa = 4.0PNTEE169 pKa = 3.69RR170 pKa = 11.84RR171 pKa = 11.84VTALFRR177 pKa = 11.84EE178 pKa = 4.6LQLTPGPCTRR188 pKa = 11.84ARR190 pKa = 11.84NRR192 pKa = 11.84GKK194 pKa = 10.85KK195 pKa = 9.9NGWPLPLDD203 pKa = 3.41WDD205 pKa = 4.19EE206 pKa = 5.62DD207 pKa = 4.45HH208 pKa = 7.42IDD210 pKa = 3.91DD211 pKa = 5.31PNYY214 pKa = 9.03QAEE217 pKa = 4.18RR218 pKa = 11.84SHH220 pKa = 5.94VRR222 pKa = 11.84SIVRR226 pKa = 11.84DD227 pKa = 3.49PEE229 pKa = 4.53RR230 pKa = 11.84IEE232 pKa = 3.58WRR234 pKa = 11.84KK235 pKa = 9.75QKK237 pKa = 10.48VAEE240 pKa = 4.18LTALGYY246 pKa = 9.82SAEE249 pKa = 4.89DD250 pKa = 2.74IAARR254 pKa = 11.84LRR256 pKa = 11.84CTARR260 pKa = 11.84TVQRR264 pKa = 11.84DD265 pKa = 3.45RR266 pKa = 11.84SALGIVEE273 pKa = 4.47RR274 pKa = 11.84EE275 pKa = 4.06QVASS279 pKa = 3.61

Molecular weight: 31.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

15489

36

2140

198.6

21.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.273 ± 0.54

0.878 ± 0.162

6.869 ± 0.435

5.849 ± 0.25

2.415 ± 0.162

8.348 ± 0.459

2.092 ± 0.219

4.487 ± 0.161

3.215 ± 0.223

7.967 ± 0.212

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.156 ± 0.15

2.892 ± 0.226

5.72 ± 0.268

3.674 ± 0.394

7.586 ± 0.472

5.087 ± 0.2

7.367 ± 0.301

6.986 ± 0.226

1.995 ± 0.15

2.143 ± 0.139
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski