Rabbit fibroma virus (strain Kasza) (RFV) (Shope fibroma virus (strain Kasza))

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes; Chitovirales; Poxviridae; Chordopoxvirinae; Leporipoxvirus; Rabbit fibroma virus

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 153 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q9Q909|VH21_RFVKA Probable host range protein 2-1 OS=Rabbit fibroma virus (strain Kasza) OX=10272 GN=s062R PE=3 SV=1
MM1 pKa = 7.69GITHH5 pKa = 7.08EE6 pKa = 4.01FHH8 pKa = 7.08IFLVDD13 pKa = 3.56EE14 pKa = 4.35NVSLKK19 pKa = 10.63SVSLLKK25 pKa = 10.63GDD27 pKa = 4.18SYY29 pKa = 11.86GCNIHH34 pKa = 6.95LKK36 pKa = 10.47NSEE39 pKa = 4.07CKK41 pKa = 10.2YY42 pKa = 9.01ITFILVLEE50 pKa = 4.69PDD52 pKa = 3.01WSEE55 pKa = 3.63IAEE58 pKa = 4.28AKK60 pKa = 9.8PIRR63 pKa = 11.84IRR65 pKa = 11.84LNGKK69 pKa = 8.97KK70 pKa = 8.93MRR72 pKa = 11.84TQLLTKK78 pKa = 9.19TLMSIIYY85 pKa = 9.36KK86 pKa = 9.91AVIYY90 pKa = 10.68VEE92 pKa = 4.7EE93 pKa = 4.06NALVQFYY100 pKa = 11.04SDD102 pKa = 3.44TDD104 pKa = 3.35RR105 pKa = 11.84LYY107 pKa = 10.44TDD109 pKa = 4.16MYY111 pKa = 8.93PTFLIDD117 pKa = 3.24MDD119 pKa = 3.75KK120 pKa = 10.39QHH122 pKa = 6.46YY123 pKa = 9.71HH124 pKa = 6.78ILDD127 pKa = 3.3NGYY130 pKa = 7.66TYY132 pKa = 10.4TYY134 pKa = 8.91IDD136 pKa = 4.05SFISEE141 pKa = 4.47CDD143 pKa = 2.99KK144 pKa = 10.97QRR146 pKa = 11.84YY147 pKa = 5.76LTSDD151 pKa = 2.65IGEE154 pKa = 4.32NGYY157 pKa = 10.6EE158 pKa = 4.09DD159 pKa = 3.63STEE162 pKa = 4.13EE163 pKa = 4.1EE164 pKa = 4.58DD165 pKa = 5.12NEE167 pKa = 4.07EE168 pKa = 4.28DD169 pKa = 3.52TDD171 pKa = 5.18GVCLYY176 pKa = 10.38CLEE179 pKa = 4.41EE180 pKa = 4.27EE181 pKa = 4.36EE182 pKa = 5.74EE183 pKa = 4.09EE184 pKa = 6.06DD185 pKa = 3.72EE186 pKa = 6.68DD187 pKa = 4.46EE188 pKa = 6.72DD189 pKa = 4.33EE190 pKa = 6.65DD191 pKa = 4.16EE192 pKa = 6.47DD193 pKa = 4.76EE194 pKa = 5.68DD195 pKa = 4.77EE196 pKa = 4.78EE197 pKa = 4.47EE198 pKa = 4.09

Molecular weight: 23.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9Q932|Q9Q932_RFVKA Gp039L OS=Rabbit fibroma virus (strain Kasza) OX=10272 GN=s039L PE=3 SV=1
MM1 pKa = 7.7ALTAKK6 pKa = 10.14EE7 pKa = 4.27VISFIWMTLLFVVMLLAGSALLFKK31 pKa = 10.17TYY33 pKa = 10.7APHH36 pKa = 6.05KK37 pKa = 10.03VVMTRR42 pKa = 11.84SAAFMRR48 pKa = 11.84VVNYY52 pKa = 10.0FEE54 pKa = 4.91LVAILVFIPGTVSLYY69 pKa = 8.71WSYY72 pKa = 11.71VKK74 pKa = 10.29SLRR77 pKa = 11.84FF78 pKa = 3.29

Molecular weight: 8.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

153

0

153

46875

32

1939

306.4

35.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.78 ± 0.116

2.17 ± 0.1

6.069 ± 0.131

5.547 ± 0.12

4.813 ± 0.16

4.049 ± 0.129

2.283 ± 0.079

8.299 ± 0.169

7.627 ± 0.171

9.154 ± 0.164

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.445 ± 0.078

6.114 ± 0.141

3.413 ± 0.111

2.236 ± 0.078

4.365 ± 0.13

7.194 ± 0.144

6.769 ± 0.143

7.479 ± 0.165

0.747 ± 0.063

5.449 ± 0.124
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski