Thermovirga lienii (strain ATCC BAA-1197 / DSM 17291 / Cas60314)

Taxonomy: cellular organisms; Bacteria; Synergistetes; Synergistia; Synergistales; Synergistaceae; Thermovirga; Thermovirga lienii

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1853 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G7V9Q1|G7V9Q1_THELD Exodeoxyribonuclease 7 large subunit OS=Thermovirga lienii (strain ATCC BAA-1197 / DSM 17291 / Cas60314) OX=580340 GN=xseA PE=3 SV=1
MM1 pKa = 7.1TSKK4 pKa = 10.61EE5 pKa = 3.88RR6 pKa = 11.84IAYY9 pKa = 10.22LEE11 pKa = 4.13GLLEE15 pKa = 4.13GLKK18 pKa = 10.47VEE20 pKa = 5.36DD21 pKa = 3.55EE22 pKa = 4.22DD23 pKa = 3.82TRR25 pKa = 11.84RR26 pKa = 11.84VYY28 pKa = 10.32EE29 pKa = 4.75AIVEE33 pKa = 4.15ALRR36 pKa = 11.84ALSSEE41 pKa = 4.04IEE43 pKa = 4.02EE44 pKa = 4.35HH45 pKa = 6.82EE46 pKa = 4.34EE47 pKa = 3.96VLTEE51 pKa = 3.86QQEE54 pKa = 4.47FLGEE58 pKa = 3.83LADD61 pKa = 3.75YY62 pKa = 11.02CEE64 pKa = 4.27EE65 pKa = 4.35LEE67 pKa = 4.15EE68 pKa = 4.61DD69 pKa = 3.46MSRR72 pKa = 11.84LEE74 pKa = 4.16EE75 pKa = 4.23GLGEE79 pKa = 3.96EE80 pKa = 4.49WEE82 pKa = 4.21EE83 pKa = 4.08EE84 pKa = 4.32EE85 pKa = 5.56YY86 pKa = 11.11EE87 pKa = 4.61DD88 pKa = 6.43DD89 pKa = 4.76EE90 pKa = 5.84DD91 pKa = 5.41LDD93 pKa = 4.48YY94 pKa = 11.32PSVTCPEE101 pKa = 4.02CGYY104 pKa = 11.13VFFYY108 pKa = 10.92DD109 pKa = 3.43PEE111 pKa = 4.25EE112 pKa = 4.49YY113 pKa = 10.42EE114 pKa = 4.24EE115 pKa = 4.88EE116 pKa = 4.55EE117 pKa = 4.12EE118 pKa = 4.51LQCPEE123 pKa = 4.83CGFLMDD129 pKa = 5.17LPKK132 pKa = 10.58EE133 pKa = 4.14

Molecular weight: 15.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G7V978|G7V978_THELD Membrane protein insertase YidC/Oxa1 family OS=Thermovirga lienii (strain ATCC BAA-1197 / DSM 17291 / Cas60314) OX=580340 GN=Tlie_0712 PE=3 SV=1
MM1 pKa = 7.38KK2 pKa = 9.6RR3 pKa = 11.84TFQPHH8 pKa = 4.74NKK10 pKa = 8.76RR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 7.53RR14 pKa = 11.84TIGFLSRR21 pKa = 11.84SRR23 pKa = 11.84SHH25 pKa = 6.55SGRR28 pKa = 11.84KK29 pKa = 8.44ILRR32 pKa = 11.84NRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.82GRR39 pKa = 11.84KK40 pKa = 8.77RR41 pKa = 11.84LAAA44 pKa = 4.42

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1853

0

1853

597420

31

1661

322.4

35.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.056 ± 0.059

1.208 ± 0.025

4.909 ± 0.04

7.697 ± 0.065

4.064 ± 0.041

7.93 ± 0.052

1.738 ± 0.022

6.659 ± 0.04

6.824 ± 0.05

10.413 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.683 ± 0.025

3.204 ± 0.03

4.372 ± 0.034

2.537 ± 0.025

5.314 ± 0.047

6.002 ± 0.047

4.444 ± 0.032

7.873 ± 0.055

1.239 ± 0.023

2.835 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski