Nocardioides dokdonensis FR1436

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides; Nocardioides dokdonensis

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4089 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1A9GP40|A0A1A9GP40_9ACTN Uncharacterized protein OS=Nocardioides dokdonensis FR1436 OX=1300347 GN=I601_2789 PE=4 SV=1
MM1 pKa = 7.8AGTAALGLVLTACGSEE17 pKa = 4.02DD18 pKa = 3.99SEE20 pKa = 5.45PEE22 pKa = 3.78ATDD25 pKa = 3.61NEE27 pKa = 4.58SSSEE31 pKa = 4.29STDD34 pKa = 3.2SAAPADD40 pKa = 4.39DD41 pKa = 4.68EE42 pKa = 4.69FTNDD46 pKa = 3.17EE47 pKa = 4.68CPNGSTSDD55 pKa = 4.0DD56 pKa = 3.61SFKK59 pKa = 11.28AGGILPLTGNLAFLGPPEE77 pKa = 4.02VAGVGMAVSDD87 pKa = 3.58INAAGGVNGADD98 pKa = 3.14ACHH101 pKa = 6.06QIEE104 pKa = 4.89DD105 pKa = 4.3SGDD108 pKa = 3.57TTDD111 pKa = 5.52LSISTASARR120 pKa = 11.84KK121 pKa = 9.63LIQDD125 pKa = 3.61KK126 pKa = 10.98ASVVIGAASSSVSLNFVDD144 pKa = 5.07RR145 pKa = 11.84LTAAKK150 pKa = 8.51ITQVSPANTAVDD162 pKa = 3.86LSGYY166 pKa = 9.7SDD168 pKa = 4.08FYY170 pKa = 11.37FRR172 pKa = 11.84TAPPDD177 pKa = 4.67GIQGNALGSLISSDD191 pKa = 3.61GFQKK195 pKa = 10.6VAFIVFSDD203 pKa = 3.98TYY205 pKa = 10.01GTGLRR210 pKa = 11.84DD211 pKa = 3.58VVQEE215 pKa = 4.16TIEE218 pKa = 4.32AGGGEE223 pKa = 4.42CTYY226 pKa = 11.01GCKK229 pKa = 10.21GDD231 pKa = 4.13GDD233 pKa = 4.34EE234 pKa = 5.39FPAGQTTFSSEE245 pKa = 3.43VSAATNSNPDD255 pKa = 3.92AIVILAFDD263 pKa = 3.78EE264 pKa = 4.52TKK266 pKa = 10.82AIVPEE271 pKa = 4.19LASTGWDD278 pKa = 3.06MSATYY283 pKa = 10.24FSDD286 pKa = 3.68GNTADD291 pKa = 3.62FSEE294 pKa = 4.76DD295 pKa = 3.37FEE297 pKa = 6.93AGTLTGAQGTIPGADD312 pKa = 3.09AAQDD316 pKa = 3.67FKK318 pKa = 11.8DD319 pKa = 5.38RR320 pKa = 11.84LVAWNEE326 pKa = 3.61YY327 pKa = 10.3AFNDD331 pKa = 3.75GLTDD335 pKa = 3.71FAYY338 pKa = 10.21AAEE341 pKa = 4.85SYY343 pKa = 10.73DD344 pKa = 3.64ATILAALAAYY354 pKa = 9.52KK355 pKa = 10.66GGATDD360 pKa = 3.88SVTVQKK366 pKa = 11.1NFAAVSGATGGEE378 pKa = 4.29EE379 pKa = 4.08CTSYY383 pKa = 11.69ADD385 pKa = 3.57CTAMLDD391 pKa = 3.41EE392 pKa = 5.03GNEE395 pKa = 3.67IRR397 pKa = 11.84YY398 pKa = 8.93VGPSGIGPIDD408 pKa = 3.94EE409 pKa = 5.0EE410 pKa = 4.57NDD412 pKa = 3.35PSSAFVGIYY421 pKa = 9.35TYY423 pKa = 11.4NEE425 pKa = 3.7NNKK428 pKa = 9.75NEE430 pKa = 4.11LTTTVEE436 pKa = 4.3GQKK439 pKa = 10.43QSS441 pKa = 3.09

Molecular weight: 45.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1A9GGE0|A0A1A9GGE0_9ACTN Orotate phosphoribosyltransferase OS=Nocardioides dokdonensis FR1436 OX=1300347 GN=pyrE_1 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.51LLKK22 pKa = 8.15KK23 pKa = 9.24TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.07LGKK33 pKa = 9.87

Molecular weight: 4.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4089

0

4089

1337234

29

1969

327.0

34.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.109 ± 0.053

0.729 ± 0.01

6.532 ± 0.028

5.9 ± 0.037

2.645 ± 0.022

9.374 ± 0.035

2.286 ± 0.021

3.047 ± 0.027

1.74 ± 0.031

10.549 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.857 ± 0.015

1.554 ± 0.018

5.724 ± 0.031

2.81 ± 0.02

7.828 ± 0.042

5.292 ± 0.024

6.035 ± 0.03

9.599 ± 0.037

1.523 ± 0.017

1.867 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski