Middle East respiratory syndrome-related coronavirus (MERS-CoV)
Average proteome isoelectric point is 7.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R9UQM6|R9UQM6_MERS NS3D protein OS=Middle East respiratory syndrome-related coronavirus OX=1335626 GN=orf5 PE=4 SV=1
MM1 pKa = 6.86 IHH3 pKa = 6.87 SVFLLMFLLTPTEE16 pKa = 4.37 SYY18 pKa = 11.55 VDD20 pKa = 3.55 VGPDD24 pKa = 3.43 SVKK27 pKa = 10.47 SACIEE32 pKa = 3.72 VDD34 pKa = 2.94 IQQTFFDD41 pKa = 4.38 KK42 pKa = 10.29 TWPRR46 pKa = 11.84 PIDD49 pKa = 3.46 VSKK52 pKa = 11.24 ADD54 pKa = 4.31 GIIYY58 pKa = 8.52 PQGRR62 pKa = 11.84 TYY64 pKa = 11.55 SNITITYY71 pKa = 8.99 QGLFPYY77 pKa = 10.39 QGDD80 pKa = 3.55 HH81 pKa = 6.45 GDD83 pKa = 3.5 MYY85 pKa = 11.3 VYY87 pKa = 10.57 SAGHH91 pKa = 5.77 ATGTTPQKK99 pKa = 10.83 LFVANYY105 pKa = 9.11 SQDD108 pKa = 3.09 VKK110 pKa = 11.08 QFANGFVVRR119 pKa = 11.84 IGAAANSTGTVIISPSTSATIRR141 pKa = 11.84 KK142 pKa = 9.31 IYY144 pKa = 8.72 PAFMLGSSVGNFSDD158 pKa = 3.45 GKK160 pKa = 8.72 MGRR163 pKa = 11.84 FFNHH167 pKa = 6.01 TLVLLPDD174 pKa = 3.85 GCGTLLRR181 pKa = 11.84 AFYY184 pKa = 10.55 CILEE188 pKa = 4.27 PRR190 pKa = 11.84 SGNHH194 pKa = 5.77 CPAGNSYY201 pKa = 9.97 TSFATYY207 pKa = 7.63 HH208 pKa = 6.01 TPATDD213 pKa = 4.12 CSDD216 pKa = 3.09 GNYY219 pKa = 9.81 NRR221 pKa = 11.84 NASLNSFKK229 pKa = 10.94 EE230 pKa = 3.91 YY231 pKa = 10.79 FNLRR235 pKa = 11.84 NCTFMYY241 pKa = 9.51 TYY243 pKa = 10.63 NITEE247 pKa = 4.15 DD248 pKa = 4.93 EE249 pKa = 3.99 ILEE252 pKa = 4.35 WFGITQTAQGVHH264 pKa = 6.39 LFSSRR269 pKa = 11.84 YY270 pKa = 7.82 VDD272 pKa = 4.34 LYY274 pKa = 9.95 GGNMFQFATLPVYY287 pKa = 9.53 DD288 pKa = 4.09 TIKK291 pKa = 9.6 YY292 pKa = 9.93 YY293 pKa = 10.88 SIIPHH298 pKa = 6.57 SIRR301 pKa = 11.84 SIQSDD306 pKa = 3.44 RR307 pKa = 11.84 KK308 pKa = 8.9 AWAAFYY314 pKa = 10.51 VYY316 pKa = 10.62 KK317 pKa = 10.57 LQPLTFLLDD326 pKa = 3.94 FSVDD330 pKa = 3.3 GYY332 pKa = 10.29 IRR334 pKa = 11.84 RR335 pKa = 11.84 AIDD338 pKa = 3.32 CGFNDD343 pKa = 5.2 LSQLHH348 pKa = 6.59 CSYY351 pKa = 11.2 EE352 pKa = 4.41 SFDD355 pKa = 3.95 VEE357 pKa = 4.4 SGVYY361 pKa = 9.77 SVSSFEE367 pKa = 4.52 AKK369 pKa = 10.07 PSGSVVEE376 pKa = 3.97 QAEE379 pKa = 4.32 GVEE382 pKa = 4.81 CDD384 pKa = 4.79 FSPLLSGTPPQVYY397 pKa = 9.55 NFKK400 pKa = 10.72 RR401 pKa = 11.84 LVFTNCNYY409 pKa = 10.82 NLTKK413 pKa = 10.6 LLSLFSVNDD422 pKa = 3.83 FTCSQISPAAIASNCYY438 pKa = 10.04 SSLILDD444 pKa = 3.82 YY445 pKa = 10.84 FSYY448 pKa = 9.64 PLSMKK453 pKa = 10.31 SDD455 pKa = 3.95 LSVSSAGPISQFNYY469 pKa = 9.2 KK470 pKa = 10.04 QSFSNPTCLILATVPHH486 pKa = 6.9 NLTTITKK493 pKa = 8.1 PLKK496 pKa = 9.3 YY497 pKa = 10.39 SYY499 pKa = 10.58 INKK502 pKa = 9.66 CSRR505 pKa = 11.84 LLSDD509 pKa = 5.19 DD510 pKa = 3.58 RR511 pKa = 11.84 TEE513 pKa = 4.39 VPQLVNANQYY523 pKa = 9.07 SPCVSIVPSTVWEE536 pKa = 4.6 DD537 pKa = 2.68 GDD539 pKa = 4.03 YY540 pKa = 10.91 YY541 pKa = 10.98 RR542 pKa = 11.84 KK543 pKa = 9.48 QLSPLEE549 pKa = 4.3 GGGWLVASGSTVAMTEE565 pKa = 3.8 QLQMGFGITVQYY577 pKa = 9.09 GTDD580 pKa = 3.57 TNSVCPKK587 pKa = 10.64 LEE589 pKa = 3.84 FANDD593 pKa = 3.52 TKK595 pKa = 10.75 IASQLGNCVEE605 pKa = 3.96 YY606 pKa = 10.83 SLYY609 pKa = 10.47 GVSGRR614 pKa = 11.84 GVFQNCTAVGVRR626 pKa = 11.84 QQRR629 pKa = 11.84 FVYY632 pKa = 10.14 DD633 pKa = 4.07 AYY635 pKa = 11.32 QNLVGYY641 pKa = 10.55 YY642 pKa = 9.85 SDD644 pKa = 4.75 DD645 pKa = 3.3 GNYY648 pKa = 9.21 YY649 pKa = 10.15 CLRR652 pKa = 11.84 ACVSVPVSVIYY663 pKa = 10.62 DD664 pKa = 3.71 KK665 pKa = 10.22 EE666 pKa = 4.71 TKK668 pKa = 8.48 THH670 pKa = 5.81 ATLFGSVACEE680 pKa = 4.33 HH681 pKa = 6.93 ISSTMSQYY689 pKa = 11.08 SRR691 pKa = 11.84 STRR694 pKa = 11.84 SMLKK698 pKa = 10.21 RR699 pKa = 11.84 RR700 pKa = 11.84 DD701 pKa = 3.4 STYY704 pKa = 11.16 GPLQTPVGCVLGLVNSSLFVEE725 pKa = 4.86 DD726 pKa = 4.29 CKK728 pKa = 11.38 LPLGQSLCALPDD740 pKa = 3.78 TPSTLTPRR748 pKa = 11.84 SVRR751 pKa = 11.84 SVPGEE756 pKa = 3.69 MRR758 pKa = 11.84 LASIAFNHH766 pKa = 7.19 PIQVDD771 pKa = 3.76 QLNSSYY777 pKa = 10.78 FKK779 pKa = 11.13 LSIPTNFSFGVTQEE793 pKa = 4.35 YY794 pKa = 10.14 IQTTIQKK801 pKa = 8.19 VTVDD805 pKa = 3.83 CKK807 pKa = 10.97 QYY809 pKa = 10.71 VCNGFQKK816 pKa = 10.67 CEE818 pKa = 3.81 QLLRR822 pKa = 11.84 EE823 pKa = 4.09 YY824 pKa = 11.27 GQFCSKK830 pKa = 10.24 INQALHH836 pKa = 5.92 GANLRR841 pKa = 11.84 QDD843 pKa = 3.46 DD844 pKa = 4.08 SVRR847 pKa = 11.84 NLFASVKK854 pKa = 9.66 SSQSSPIIPGFGGDD868 pKa = 3.83 FNLTLLEE875 pKa = 4.3 PVSISTGSRR884 pKa = 11.84 SARR887 pKa = 11.84 SAIEE891 pKa = 4.69 DD892 pKa = 3.78 LLFDD896 pKa = 4.46 KK897 pKa = 10.28 VTIADD902 pKa = 3.68 PGYY905 pKa = 8.03 MQGYY909 pKa = 9.01 DD910 pKa = 3.52 DD911 pKa = 6.26 CMQQGPASARR921 pKa = 11.84 DD922 pKa = 4.26 LICAQYY928 pKa = 9.2 VAGYY932 pKa = 9.81 KK933 pKa = 9.75 VLPPLMDD940 pKa = 3.55 VNMEE944 pKa = 4.0 AAYY947 pKa = 9.29 TSSLLGSIAGVGWTAGLSSFAAIPFAQSIFYY978 pKa = 10.26 RR979 pKa = 11.84 LNGVGITQQVLSEE992 pKa = 4.06 NQKK995 pKa = 10.94 LIANKK1000 pKa = 9.66 FNQALGAMQTGFTTTNEE1017 pKa = 4.13 AFRR1020 pKa = 11.84 KK1021 pKa = 9.46 VQDD1024 pKa = 3.57 AVNNNAQALSKK1035 pKa = 10.55 LASEE1039 pKa = 5.11 LSNTFGAISASIGDD1053 pKa = 4.24 IIQRR1057 pKa = 11.84 LDD1059 pKa = 3.4 VLEE1062 pKa = 4.26 QDD1064 pKa = 3.65 AQIDD1068 pKa = 3.67 RR1069 pKa = 11.84 LINGRR1074 pKa = 11.84 LTTLNAFVAQQLVRR1088 pKa = 11.84 SEE1090 pKa = 4.28 SAALSAQLAKK1100 pKa = 10.93 DD1101 pKa = 3.6 KK1102 pKa = 11.5 VNEE1105 pKa = 4.19 CVKK1108 pKa = 10.57 AQSKK1112 pKa = 10.61 RR1113 pKa = 11.84 SGFCGQGTHH1122 pKa = 5.83 IVSFVVNAPNGLYY1135 pKa = 10.31 FMHH1138 pKa = 6.54 VGYY1141 pKa = 10.68 YY1142 pKa = 8.92 PSNHH1146 pKa = 6.15 IEE1148 pKa = 4.11 VVSAYY1153 pKa = 9.92 GLCDD1157 pKa = 3.03 AANPTNCIAPVNGYY1171 pKa = 9.08 FIKK1174 pKa = 10.17 TNNTRR1179 pKa = 11.84 IVDD1182 pKa = 3.46 EE1183 pKa = 4.15 WSYY1186 pKa = 10.86 TGSSFYY1192 pKa = 11.03 APEE1195 pKa = 5.67 PITSLNTKK1203 pKa = 9.23 YY1204 pKa = 10.36 VAPHH1208 pKa = 4.7 VTYY1211 pKa = 11.0 QNISTNLPPPLLGNSTGIDD1230 pKa = 3.71 FQDD1233 pKa = 3.67 EE1234 pKa = 3.86 LDD1236 pKa = 3.71 EE1237 pKa = 4.43 FFKK1240 pKa = 11.2 NVSTSIPNFGSLTQINTTLLDD1261 pKa = 3.48 LTYY1264 pKa = 11.2 EE1265 pKa = 4.16 MLSLQQVVKK1274 pKa = 10.52 ALNEE1278 pKa = 4.09 SYY1280 pKa = 10.82 IDD1282 pKa = 3.9 LKK1284 pKa = 11.28 EE1285 pKa = 4.19 LGNYY1289 pKa = 7.68 TYY1291 pKa = 11.04 YY1292 pKa = 11.18 NKK1294 pKa = 9.72 WPWYY1298 pKa = 8.04 IWLGFIAGLVALALCVFFILCCTGCGTNCMGKK1330 pKa = 9.91 LKK1332 pKa = 10.64 CNRR1335 pKa = 11.84 CCDD1338 pKa = 3.3 RR1339 pKa = 11.84 YY1340 pKa = 10.78 EE1341 pKa = 4.28 EE1342 pKa = 4.54 YY1343 pKa = 10.79 DD1344 pKa = 3.78 LEE1346 pKa = 4.13 PHH1348 pKa = 6.39 KK1349 pKa = 11.21 VHH1351 pKa = 6.25 VHH1353 pKa = 5.33
Molecular weight: 149.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.394
IPC2_protein 5.499
IPC_protein 5.537
Toseland 5.728
ProMoST 5.792
Dawson 5.741
Bjellqvist 5.779
Wikipedia 5.728
Rodwell 5.703
Grimsley 5.792
Solomon 5.741
Lehninger 5.728
Nozaki 5.982
DTASelect 6.173
Thurlkill 6.109
EMBOSS 6.071
Sillero 6.071
Patrickios 0.744
IPC_peptide 5.753
IPC2_peptide 6.071
IPC2.peptide.svr19 5.939
Protein with the highest isoelectric point:
>tr|T2BBK0|T2BBK0_MERS Nucleoprotein OS=Middle East respiratory syndrome-related coronavirus OX=1335626 GN=N PE=3 SV=1
MM1 pKa = 7.86 ASPAAPRR8 pKa = 11.84 AVSFADD14 pKa = 3.78 NNDD17 pKa = 3.02 ITNTNLSRR25 pKa = 11.84 GRR27 pKa = 11.84 GRR29 pKa = 11.84 NPKK32 pKa = 9.26 PRR34 pKa = 11.84 AAPNNTVSWYY44 pKa = 9.65 TGLTQHH50 pKa = 6.38 GKK52 pKa = 10.01 VPLTFPPGQGVPLNANSTPAQNAGYY77 pKa = 9.33 WRR79 pKa = 11.84 RR80 pKa = 11.84 QDD82 pKa = 3.96 RR83 pKa = 11.84 KK84 pKa = 9.7 INTGNGIKK92 pKa = 9.96 QLAPRR97 pKa = 11.84 WYY99 pKa = 9.84 FYY101 pKa = 10.45 YY102 pKa = 10.42 TGTGPEE108 pKa = 3.59 AALPFRR114 pKa = 11.84 AVKK117 pKa = 10.58 DD118 pKa = 4.02 GIVWVHH124 pKa = 5.14 EE125 pKa = 4.51 HH126 pKa = 6.58 GATDD130 pKa = 3.5 APSTFGTRR138 pKa = 11.84 NPNNDD143 pKa = 3.31 SAIVTQFAPGTKK155 pKa = 9.59 LPKK158 pKa = 9.89 NFHH161 pKa = 6.42 IEE163 pKa = 4.18 GTGGNSQSSSRR174 pKa = 11.84 ASSVSRR180 pKa = 11.84 NSSRR184 pKa = 11.84 SSSQGSRR191 pKa = 11.84 SGNSTRR197 pKa = 11.84 GTSPGPSGIGAVGGDD212 pKa = 4.31 LLYY215 pKa = 11.06 LDD217 pKa = 5.07 LLNRR221 pKa = 11.84 LQALEE226 pKa = 4.11 SGKK229 pKa = 10.41 VKK231 pKa = 10.49 QSQPKK236 pKa = 10.37 VITKK240 pKa = 10.17 KK241 pKa = 10.58 DD242 pKa = 2.86 AAAAKK247 pKa = 10.32 NKK249 pKa = 8.51 MRR251 pKa = 11.84 HH252 pKa = 5.56 KK253 pKa = 9.8 RR254 pKa = 11.84 TSTKK258 pKa = 10.27 SFNMVQAFGLRR269 pKa = 11.84 GPGDD273 pKa = 3.51 LQGNFGDD280 pKa = 4.05 LQLNKK285 pKa = 10.39 LGTEE289 pKa = 4.19 DD290 pKa = 4.25 PRR292 pKa = 11.84 WPQIAEE298 pKa = 4.14 LAPTASAFMGMSQFKK313 pKa = 8.94 LTHH316 pKa = 5.96 QNNDD320 pKa = 2.91 DD321 pKa = 4.12 HH322 pKa = 6.97 GNPVYY327 pKa = 10.43 FLRR330 pKa = 11.84 YY331 pKa = 9.33 SGAIKK336 pKa = 10.61 LDD338 pKa = 3.71 PKK340 pKa = 10.57 NPNYY344 pKa = 10.63 NKK346 pKa = 9.76 WLEE349 pKa = 3.97 LLEE352 pKa = 4.17 QNIDD356 pKa = 3.26 AYY358 pKa = 9.71 KK359 pKa = 10.18 TFPKK363 pKa = 10.2 KK364 pKa = 9.89 EE365 pKa = 4.15 KK366 pKa = 9.67 KK367 pKa = 9.72 QKK369 pKa = 10.33 APKK372 pKa = 10.05 EE373 pKa = 3.99 EE374 pKa = 4.54 STDD377 pKa = 3.68 QMSEE381 pKa = 3.88 PPKK384 pKa = 8.43 EE385 pKa = 3.81 QRR387 pKa = 11.84 VQGSITQRR395 pKa = 11.84 TRR397 pKa = 11.84 TRR399 pKa = 11.84 PSVQPGPMIDD409 pKa = 3.5 VNTDD413 pKa = 2.58
Molecular weight: 45.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.154
IPC2_protein 9.458
IPC_protein 9.531
Toseland 10.365
ProMoST 9.926
Dawson 10.482
Bjellqvist 10.101
Wikipedia 10.613
Rodwell 10.994
Grimsley 10.526
Solomon 10.511
Lehninger 10.482
Nozaki 10.321
DTASelect 10.101
Thurlkill 10.35
EMBOSS 10.73
Sillero 10.394
Patrickios 10.687
IPC_peptide 10.511
IPC2_peptide 8.536
IPC2.peptide.svr19 8.631
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11
0
11
14330
82
7078
1302.7
144.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.264 ± 0.276
3.001 ± 0.308
5.304 ± 0.332
3.88 ± 0.195
5.045 ± 0.17
5.827 ± 0.241
2.066 ± 0.172
4.801 ± 0.183
5.255 ± 0.352
9.463 ± 0.382
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.247 ± 0.148
4.997 ± 0.161
4.215 ± 0.459
3.489 ± 0.301
3.643 ± 0.292
7.704 ± 0.511
6.936 ± 0.1
9.016 ± 0.587
1.137 ± 0.089
4.71 ± 0.242
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here