Campylobacter pinnipediorum subsp. caledonicus
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1649 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1S6U8U0|A0A1S6U8U0_9PROT Outer membrane protein assembly factor BamA OS=Campylobacter pinnipediorum subsp. caledonicus OX=1874362 GN=bamA PE=3 SV=1
MM1 pKa = 7.6 TYY3 pKa = 10.93 DD4 pKa = 3.86 EE5 pKa = 5.47 LEE7 pKa = 4.27 LDD9 pKa = 3.73 AVLLEE14 pKa = 3.9 ILEE17 pKa = 4.52 NNGSFDD23 pKa = 4.02 SLDD26 pKa = 3.86 DD27 pKa = 3.85 EE28 pKa = 4.65 EE29 pKa = 6.12 LYY31 pKa = 11.09 EE32 pKa = 5.65 LIEE35 pKa = 4.3 QVAQYY40 pKa = 10.44 TDD42 pKa = 3.4 GDD44 pKa = 3.62 IEE46 pKa = 4.17 EE47 pKa = 4.58 AFEE50 pKa = 4.27 YY51 pKa = 7.54 MTQFSPIPKK60 pKa = 9.54 KK61 pKa = 10.6 RR62 pKa = 11.84 FASLFMVV69 pKa = 4.28
Molecular weight: 8.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.885
IPC2_protein 3.592
IPC_protein 3.49
Toseland 3.312
ProMoST 3.668
Dawson 3.478
Bjellqvist 3.643
Wikipedia 3.401
Rodwell 3.338
Grimsley 3.236
Solomon 3.427
Lehninger 3.389
Nozaki 3.617
DTASelect 3.732
Thurlkill 3.376
EMBOSS 3.414
Sillero 3.605
Patrickios 1.761
IPC_peptide 3.427
IPC2_peptide 3.579
IPC2.peptide.svr19 3.66
Protein with the highest isoelectric point:
>tr|A0A1S6U7F1|A0A1S6U7F1_9PROT ABC transporter ATP-binding protein OS=Campylobacter pinnipediorum subsp. caledonicus OX=1874362 GN=CPIN18021_0561 PE=4 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.06 QPHH8 pKa = 5.76 KK9 pKa = 7.92 TPKK12 pKa = 9.96 KK13 pKa = 7.47 RR14 pKa = 11.84 THH16 pKa = 5.94 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MKK23 pKa = 9.07 TKK25 pKa = 10.4 NGRR28 pKa = 11.84 KK29 pKa = 9.05 VLNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.84 GRR39 pKa = 11.84 RR40 pKa = 11.84 RR41 pKa = 11.84 LAVV44 pKa = 3.15
Molecular weight: 5.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.415
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.398
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.135
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.013
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1649
0
1649
524899
37
5374
318.3
35.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.359 ± 0.067
1.048 ± 0.029
6.16 ± 0.048
6.427 ± 0.072
5.43 ± 0.073
5.829 ± 0.057
1.439 ± 0.026
9.185 ± 0.075
9.657 ± 0.085
9.352 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.311 ± 0.032
6.58 ± 0.069
2.653 ± 0.032
2.873 ± 0.029
3.093 ± 0.041
6.833 ± 0.049
4.661 ± 0.055
5.823 ± 0.051
0.623 ± 0.019
3.664 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here