Formivibrio citricus
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2769 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I4WG81|A0A1I4WG81_9NEIS GTPase Era OS=Formivibrio citricus OX=83765 GN=era PE=3 SV=1
MM1 pKa = 7.01 STATDD6 pKa = 3.68 MPSPFIFTDD15 pKa = 3.6 AAAEE19 pKa = 4.16 KK20 pKa = 10.33 VRR22 pKa = 11.84 EE23 pKa = 4.34 LIQEE27 pKa = 4.17 EE28 pKa = 4.78 GNPDD32 pKa = 2.91 LKK34 pKa = 11.14 LRR36 pKa = 11.84 VFVTGGGCSGFQYY49 pKa = 10.9 GFTFDD54 pKa = 4.44 EE55 pKa = 4.53 VANDD59 pKa = 3.83 DD60 pKa = 4.03 DD61 pKa = 4.03 ATVVKK66 pKa = 10.82 NGVTLLVDD74 pKa = 3.69 PMSYY78 pKa = 10.34 QYY80 pKa = 11.57 LVGAEE85 pKa = 3.69 IDD87 pKa = 3.85 YY88 pKa = 11.28 VEE90 pKa = 4.54 SLEE93 pKa = 4.67 GSQFTIKK100 pKa = 10.63 NPNAVSTCGCGSSFSVV116 pKa = 3.54
Molecular weight: 12.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.764
IPC2_protein 3.923
IPC_protein 3.846
Toseland 3.656
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.757
Rodwell 3.681
Grimsley 3.567
Solomon 3.821
Lehninger 3.77
Nozaki 3.961
DTASelect 4.151
Thurlkill 3.706
EMBOSS 3.77
Sillero 3.973
Patrickios 1.888
IPC_peptide 3.808
IPC2_peptide 3.948
IPC2.peptide.svr19 3.866
Protein with the highest isoelectric point:
>tr|A0A1I4Z4F1|A0A1I4Z4F1_9NEIS DNA helicase OS=Formivibrio citricus OX=83765 GN=SAMN05660284_01515 PE=4 SV=1
MM1 pKa = 7.38 NATTVAVDD9 pKa = 3.61 LAKK12 pKa = 10.57 SVFRR16 pKa = 11.84 IAVADD21 pKa = 3.82 SAWRR25 pKa = 11.84 VVEE28 pKa = 3.69 QHH30 pKa = 6.82 RR31 pKa = 11.84 LTRR34 pKa = 11.84 CQFEE38 pKa = 3.81 RR39 pKa = 11.84 WFANRR44 pKa = 11.84 NVSLVVMEE52 pKa = 4.68 ACGSAHH58 pKa = 6.66 HH59 pKa = 6.49 WARR62 pKa = 11.84 WLNGQGIEE70 pKa = 4.58 VRR72 pKa = 11.84 LLPAAYY78 pKa = 8.65 IRR80 pKa = 11.84 AYY82 pKa = 9.91 VRR84 pKa = 11.84 RR85 pKa = 11.84 NKK87 pKa = 9.44 TDD89 pKa = 3.27 AADD92 pKa = 3.49 ACALLEE98 pKa = 4.14 AARR101 pKa = 11.84 CADD104 pKa = 3.45 IQPVKK109 pKa = 10.19 IKK111 pKa = 10.68 SVEE114 pKa = 3.96 QQALQGLHH122 pKa = 5.42 RR123 pKa = 11.84 TRR125 pKa = 11.84 SLWMARR131 pKa = 11.84 RR132 pKa = 11.84 TSRR135 pKa = 11.84 INALRR140 pKa = 11.84 GFCRR144 pKa = 11.84 EE145 pKa = 3.78 FGIVLSTGSRR155 pKa = 11.84 VGIEE159 pKa = 3.86 QISRR163 pKa = 11.84 VLADD167 pKa = 3.87 PASAVPGLIRR177 pKa = 11.84 GTMKK181 pKa = 10.92 LLVEE185 pKa = 4.88 EE186 pKa = 4.42 IRR188 pKa = 11.84 LLEE191 pKa = 3.98 ARR193 pKa = 11.84 ISQLEE198 pKa = 4.09 RR199 pKa = 11.84 EE200 pKa = 4.46 LTEE203 pKa = 3.88 IARR206 pKa = 11.84 QSAACTLLMSIPGVGLLTATAMVAATSGHH235 pKa = 5.22 VEE237 pKa = 3.96 HH238 pKa = 6.95 FKK240 pKa = 10.61 DD241 pKa = 3.65 ARR243 pKa = 11.84 HH244 pKa = 5.01 FASWFGLTPKK254 pKa = 9.47 EE255 pKa = 4.07 HH256 pKa = 7.03 SSGSTRR262 pKa = 11.84 KK263 pKa = 9.72 LGRR266 pKa = 11.84 ISKK269 pKa = 10.43 QGDD272 pKa = 3.25 RR273 pKa = 11.84 YY274 pKa = 11.0 LRR276 pKa = 11.84 MLLTHH281 pKa = 6.65 GARR284 pKa = 11.84 AVLRR288 pKa = 11.84 SASMAKK294 pKa = 8.9 TAGRR298 pKa = 11.84 PLNVLRR304 pKa = 11.84 QWALDD309 pKa = 3.42 VQARR313 pKa = 11.84 TNHH316 pKa = 5.41 NKK318 pKa = 9.38 AACALANKK326 pKa = 9.54 LARR329 pKa = 11.84 ICYY332 pKa = 7.48 ATLRR336 pKa = 11.84 DD337 pKa = 3.57 CCGFEE342 pKa = 3.83 EE343 pKa = 4.73 TVRR346 pKa = 11.84 LQRR349 pKa = 11.84 KK350 pKa = 6.33 LQRR353 pKa = 11.84 TAFEE357 pKa = 4.3 MPAA360 pKa = 3.26
Molecular weight: 40.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.354
IPC2_protein 9.472
IPC_protein 10.262
Toseland 10.701
ProMoST 10.438
Dawson 10.774
Bjellqvist 10.511
Wikipedia 10.994
Rodwell 10.891
Grimsley 10.804
Solomon 10.935
Lehninger 10.891
Nozaki 10.716
DTASelect 10.496
Thurlkill 10.701
EMBOSS 11.111
Sillero 10.716
Patrickios 10.599
IPC_peptide 10.935
IPC2_peptide 9.838
IPC2.peptide.svr19 8.653
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2769
0
2769
913534
16
2274
329.9
36.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.199 ± 0.054
1.069 ± 0.017
5.121 ± 0.032
6.296 ± 0.046
3.737 ± 0.028
7.656 ± 0.037
2.229 ± 0.024
5.346 ± 0.037
4.291 ± 0.044
11.03 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.503 ± 0.022
3.154 ± 0.03
4.744 ± 0.035
4.057 ± 0.036
6.426 ± 0.038
5.442 ± 0.035
4.866 ± 0.032
7.117 ± 0.04
1.296 ± 0.021
2.422 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here