Nocardia pseudobrasiliensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae;

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7943 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A370HXT5|A0A370HXT5_9NOCA Bacterial proteasome activator OS=Nocardia pseudobrasiliensis OX=45979 GN=DFR76_113246 PE=3 SV=1
MM1 pKa = 7.47SPNAILEE8 pKa = 4.84FILSLLRR15 pKa = 11.84DD16 pKa = 3.39HH17 pKa = 6.86EE18 pKa = 4.31AAVNYY23 pKa = 8.26CANPAAALTAAGLEE37 pKa = 4.21AVTPEE42 pKa = 5.07DD43 pKa = 3.4IAAVAPMVAEE53 pKa = 4.18SALVSGGSQLAAIVAAGAGAAASAGLNAVASAATDD88 pKa = 3.57ANLGAGLAAGVNGGAQAGTGLDD110 pKa = 3.16IGLSTGADD118 pKa = 3.19LGAGLSAGLQTAAGLGAGLSAGLGAAAAAGTGAVAGLEE156 pKa = 4.28AGLSAGLGALAGTAASLSQTVSAGANLNTDD186 pKa = 3.01IGADD190 pKa = 3.57LTAGLSTTAAALTDD204 pKa = 3.55ATADD208 pKa = 3.37LTGAINGTLGGITDD222 pKa = 3.96ATTHH226 pKa = 6.81LGTDD230 pKa = 3.6LSAAVNSATHH240 pKa = 5.82VGTDD244 pKa = 3.5LGAALGGSVNTALTGATNIGASLSSGLEE272 pKa = 3.96TGLSGATGVAGDD284 pKa = 4.83LGATLGGAASTGAQLGGNLSGALDD308 pKa = 4.12GAAHH312 pKa = 7.03AGSDD316 pKa = 3.69LGAALGGSLTGGIDD330 pKa = 3.36TGLHH334 pKa = 6.37AGTDD338 pKa = 3.62LGTGLSTGINGTLDD352 pKa = 3.15GATQIGTGLEE362 pKa = 4.17TGLSSGVNGALGGAAQVGTGLSTGVNGAIDD392 pKa = 4.31GVAHH396 pKa = 7.33AGTSLEE402 pKa = 4.51SGLSTGVDD410 pKa = 3.57GALGGAAHH418 pKa = 7.56AGTGLEE424 pKa = 4.48SGLSTGVDD432 pKa = 3.57GALGGAAHH440 pKa = 6.82VGTGLEE446 pKa = 4.29SWLSTGVNGALGGAAHH462 pKa = 7.56AGTGLEE468 pKa = 4.48SGLSTGVDD476 pKa = 3.57GALGGAAHH484 pKa = 6.82VGTGLEE490 pKa = 4.43SGLSTGVNGALGGAAHH506 pKa = 7.56AGTGLEE512 pKa = 4.49SGLSTGIGGALDD524 pKa = 3.68TGLHH528 pKa = 6.25AGGDD532 pKa = 3.69LGAGVTGGVDD542 pKa = 3.57TAAHH546 pKa = 6.12GASNLGGDD554 pKa = 4.49LSTSVGGGANPWAGVDD570 pKa = 3.58VSHH573 pKa = 7.3AFGSGVGASFGAGGLDD589 pKa = 3.4GSLGGSANGAIDD601 pKa = 3.5STLDD605 pKa = 3.42AGGHH609 pKa = 5.73GSLGGGLDD617 pKa = 3.74TTGHH621 pKa = 6.65GSTSLFGDD629 pKa = 4.0TGATATADD637 pKa = 3.3THH639 pKa = 7.03AGGDD643 pKa = 3.36ITGGLLHH650 pKa = 7.16

Molecular weight: 57.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A370I6W6|A0A370I6W6_9NOCA Phospholipid/cholesterol/gamma-HCH transport system ATP-binding protein OS=Nocardia pseudobrasiliensis OX=45979 GN=DFR76_104236 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.72HH17 pKa = 5.63RR18 pKa = 11.84KK19 pKa = 7.56LLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.11LGKK33 pKa = 9.87

Molecular weight: 4.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7943

0

7943

2519924

29

14540

317.3

34.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.224 ± 0.038

0.781 ± 0.01

6.096 ± 0.024

5.423 ± 0.031

2.976 ± 0.017

8.873 ± 0.027

2.246 ± 0.016

4.267 ± 0.019

1.848 ± 0.021

10.289 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.877 ± 0.011

2.051 ± 0.015

5.925 ± 0.026

2.819 ± 0.016

8.017 ± 0.033

5.178 ± 0.02

6.066 ± 0.022

8.392 ± 0.031

1.507 ± 0.012

2.146 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski