Cardiobacterium hominis (strain ATCC 15826 / DSM 8339 / NCTC 10426 / 6573)
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2580 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C8NCV9|C8NCV9_CARH6 Type III pantothenate kinase OS=Cardiobacterium hominis (strain ATCC 15826 / DSM 8339 / NCTC 10426 / 6573) OX=638300 GN=coaX PE=3 SV=1
MM1 pKa = 7.24 AQITLTLNDD10 pKa = 3.19 SSGRR14 pKa = 11.84 AVAQYY19 pKa = 11.48 NLDD22 pKa = 3.72 TAGSALHH29 pKa = 6.73 IPAEE33 pKa = 4.35 NGVSYY38 pKa = 10.86 QFTDD42 pKa = 3.36 LATGLGPQNVTTAQQGSDD60 pKa = 3.58 LLVSFDD66 pKa = 3.89 SGTDD70 pKa = 3.53 LVIEE74 pKa = 4.39 NYY76 pKa = 9.9 FGQGQGALVGVNADD90 pKa = 4.1 GGLTSYY96 pKa = 10.55 PVTAAPEE103 pKa = 3.77 HH104 pKa = 6.14 ALASEE109 pKa = 4.53 VAAGQTLGSDD119 pKa = 3.97 QPPLAPLAVVGGIGG133 pKa = 3.55
Molecular weight: 13.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.83
IPC2_protein 3.643
IPC_protein 3.567
Toseland 3.376
ProMoST 3.783
Dawson 3.592
Bjellqvist 3.757
Wikipedia 3.579
Rodwell 3.414
Grimsley 3.287
Solomon 3.541
Lehninger 3.503
Nozaki 3.732
DTASelect 3.948
Thurlkill 3.465
EMBOSS 3.579
Sillero 3.706
Patrickios 0.299
IPC_peptide 3.541
IPC2_peptide 3.668
IPC2.peptide.svr19 3.666
Protein with the highest isoelectric point:
>tr|C8N7T4|C8N7T4_CARH6 CarD-like protein (Fragment) OS=Cardiobacterium hominis (strain ATCC 15826 / DSM 8339 / NCTC 10426 / 6573) OX=638300 GN=mfd PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSNLSRR13 pKa = 11.84 KK14 pKa = 7.51 RR15 pKa = 11.84 THH17 pKa = 6.36 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MATKK26 pKa = 10.39 NGRR29 pKa = 11.84 LVLKK33 pKa = 10.32 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 9.98 GRR40 pKa = 11.84 HH41 pKa = 5.34 RR42 pKa = 11.84 LTVV45 pKa = 3.07
Molecular weight: 5.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2580
0
2580
770704
39
2477
298.7
32.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.156 ± 0.081
0.895 ± 0.018
5.729 ± 0.05
5.439 ± 0.056
3.465 ± 0.037
7.443 ± 0.064
2.625 ± 0.032
5.786 ± 0.037
4.134 ± 0.043
10.56 ± 0.088
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.123 ± 0.026
3.653 ± 0.048
4.845 ± 0.042
4.196 ± 0.036
6.117 ± 0.052
5.016 ± 0.04
5.386 ± 0.051
5.839 ± 0.051
1.357 ± 0.027
3.234 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here