Pseudoxanthomonas spadix (strain BD-a59)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Pseudoxanthomonas; Pseudoxanthomonas spadix

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3147 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G7URL0|G7URL0_PSEUP Aminotransferase AlaT OS=Pseudoxanthomonas spadix (strain BD-a59) OX=1045855 GN=DSC_00160 PE=4 SV=1
MM1 pKa = 7.35SKK3 pKa = 10.59KK4 pKa = 10.17LRR6 pKa = 11.84YY7 pKa = 9.58ALIVALAALPFASSAQEE24 pKa = 3.63AVAEE28 pKa = 4.4EE29 pKa = 4.49EE30 pKa = 5.53AEE32 pKa = 4.78SPWAWSLTATTDD44 pKa = 3.08YY45 pKa = 10.94RR46 pKa = 11.84FRR48 pKa = 11.84GLSQTDD54 pKa = 3.37VGPAFQPGLTYY65 pKa = 7.51TTPIGIYY72 pKa = 10.09AGVWASNVDD81 pKa = 4.19FGEE84 pKa = 4.29GDD86 pKa = 3.48PDD88 pKa = 4.06YY89 pKa = 11.33EE90 pKa = 3.9VDD92 pKa = 3.52TFVGYY97 pKa = 10.85NIDD100 pKa = 3.81ANDD103 pKa = 3.85WLNLDD108 pKa = 4.07FMVNRR113 pKa = 11.84YY114 pKa = 8.82NYY116 pKa = 9.54PDD118 pKa = 4.21AGSGNYY124 pKa = 9.58NEE126 pKa = 5.1YY127 pKa = 10.17IGKK130 pKa = 8.73ATMAEE135 pKa = 4.31HH136 pKa = 5.75FTVLVGYY143 pKa = 8.87TNDD146 pKa = 3.33VFKK149 pKa = 11.16SDD151 pKa = 3.52TDD153 pKa = 2.74SWYY156 pKa = 9.59YY157 pKa = 10.0QGGISWDD164 pKa = 3.69LPQDD168 pKa = 3.69FSVAATGGVTEE179 pKa = 5.04FEE181 pKa = 5.03DD182 pKa = 4.08GTVGRR187 pKa = 11.84DD188 pKa = 3.4YY189 pKa = 11.45KK190 pKa = 10.92DD191 pKa = 2.94WSVSLSKK198 pKa = 11.11AFGPATLTAAYY209 pKa = 9.36IGTDD213 pKa = 2.99GDD215 pKa = 3.68GRR217 pKa = 11.84DD218 pKa = 3.79AYY220 pKa = 10.89GDD222 pKa = 3.51IADD225 pKa = 3.78SRR227 pKa = 11.84LVVSVTVANN236 pKa = 4.01

Molecular weight: 25.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G7UWH8|G7UWH8_PSEUP Alpha/beta hydrolase fold protein OS=Pseudoxanthomonas spadix (strain BD-a59) OX=1045855 GN=DSC_09175 PE=4 SV=1
MM1 pKa = 7.5LRR3 pKa = 11.84TLALTGLSLVLLLPGGRR20 pKa = 11.84GSTEE24 pKa = 3.82LLGWTPLWLAGLPLVAWWSLHH45 pKa = 5.82RR46 pKa = 11.84FRR48 pKa = 11.84LPAWPKK54 pKa = 8.39TRR56 pKa = 11.84PAARR60 pKa = 11.84AALAVSVPSARR71 pKa = 11.84VAVQARR77 pKa = 11.84RR78 pKa = 11.84RR79 pKa = 11.84RR80 pKa = 11.84PRR82 pKa = 11.84PARR85 pKa = 11.84MPRR88 pKa = 11.84AAA90 pKa = 4.66

Molecular weight: 9.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3147

0

3147

1003021

26

2887

318.7

34.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.297 ± 0.061

0.842 ± 0.014

5.614 ± 0.036

5.087 ± 0.041

3.291 ± 0.026

8.513 ± 0.04

2.322 ± 0.02

4.114 ± 0.023

2.612 ± 0.033

11.148 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.177 ± 0.018

2.334 ± 0.027

5.498 ± 0.036

4.394 ± 0.031

7.661 ± 0.042

5.047 ± 0.029

4.892 ± 0.028

7.383 ± 0.033

1.508 ± 0.021

2.265 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski